Experiment: nitrogen source nitrite 0.1mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3535 and Pf1N1B4_3536 are separated by 162 nucleotides Pf1N1B4_3536 and Pf1N1B4_3537 overlap by 7 nucleotides Pf1N1B4_3537 and Pf1N1B4_3538 are separated by 292 nucleotides
Pf1N1B4_3535 - Tfp pilus assembly protein FimV, at 3,941,730 to 3,943,547
_3535
Pf1N1B4_3536 - SelT/selW/selH selenoprotein domain, at 3,943,710 to 3,943,997
_3536
Pf1N1B4_3537 - Permease of the drug/metabolite transporter (DMT) superfamily, at 3,943,991 to 3,944,884
_3537
Pf1N1B4_3538 - Transcriptional regulator, AraC family, at 3,945,177 to 3,945,935
_3538
Position (kb)
3943
3944
3945 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3943.216 kb on + strand, within Pf1N1B4_3535 at 3943.216 kb on + strand, within Pf1N1B4_3535 at 3943.216 kb on + strand, within Pf1N1B4_3535 at 3943.216 kb on + strand, within Pf1N1B4_3535 at 3943.216 kb on + strand at 3943.216 kb on + strand at 3943.216 kb on + strand, within Pf1N1B4_3535 at 3943.216 kb on + strand, within Pf1N1B4_3535 at 3943.216 kb on + strand, within Pf1N1B4_3535 at 3943.216 kb on + strand, within Pf1N1B4_3535 at 3943.216 kb on + strand, within Pf1N1B4_3535 at 3943.216 kb on + strand, within Pf1N1B4_3535 at 3943.217 kb on - strand, within Pf1N1B4_3535 at 3943.217 kb on - strand, within Pf1N1B4_3535 at 3943.217 kb on - strand, within Pf1N1B4_3535 at 3943.217 kb on - strand, within Pf1N1B4_3535 at 3943.217 kb on - strand, within Pf1N1B4_3535 at 3943.217 kb on - strand, within Pf1N1B4_3535 at 3943.217 kb on - strand, within Pf1N1B4_3535 at 3943.563 kb on - strand at 3943.563 kb on - strand at 3943.563 kb on - strand at 3943.563 kb on - strand at 3943.563 kb on - strand at 3943.647 kb on + strand at 3943.647 kb on + strand at 3943.721 kb on + strand at 3943.721 kb on + strand at 3943.743 kb on + strand, within Pf1N1B4_3536 at 3943.743 kb on + strand, within Pf1N1B4_3536 at 3943.743 kb on + strand, within Pf1N1B4_3536 at 3943.744 kb on - strand, within Pf1N1B4_3536 at 3943.744 kb on - strand, within Pf1N1B4_3536 at 3943.744 kb on - strand, within Pf1N1B4_3536 at 3943.744 kb on - strand, within Pf1N1B4_3536 at 3943.744 kb on - strand, within Pf1N1B4_3536 at 3943.802 kb on + strand, within Pf1N1B4_3536 at 3943.802 kb on + strand, within Pf1N1B4_3536 at 3943.802 kb on + strand, within Pf1N1B4_3536 at 3944.279 kb on + strand, within Pf1N1B4_3537 at 3944.279 kb on + strand, within Pf1N1B4_3537 at 3944.280 kb on - strand, within Pf1N1B4_3537 at 3944.288 kb on + strand, within Pf1N1B4_3537 at 3944.289 kb on - strand, within Pf1N1B4_3537 at 3944.289 kb on - strand, within Pf1N1B4_3537 at 3944.289 kb on - strand, within Pf1N1B4_3537 at 3944.289 kb on - strand, within Pf1N1B4_3537 at 3944.495 kb on - strand, within Pf1N1B4_3537 at 3944.495 kb on - strand, within Pf1N1B4_3537 at 3944.495 kb on - strand, within Pf1N1B4_3537 at 3944.498 kb on - strand, within Pf1N1B4_3537 at 3944.898 kb on - strand at 3944.935 kb on - strand at 3944.935 kb on - strand at 3945.005 kb on - strand at 3945.005 kb on - strand at 3945.130 kb on + strand at 3945.130 kb on + strand at 3945.136 kb on + strand at 3945.137 kb on - strand at 3945.155 kb on + strand at 3945.156 kb on - strand at 3945.156 kb on - strand at 3945.156 kb on - strand at 3945.352 kb on + strand, within Pf1N1B4_3538 at 3945.353 kb on - strand, within Pf1N1B4_3538 at 3945.353 kb on - strand at 3945.353 kb on - strand, within Pf1N1B4_3538 at 3945.460 kb on + strand, within Pf1N1B4_3538 at 3945.490 kb on + strand, within Pf1N1B4_3538 at 3945.490 kb on + strand, within Pf1N1B4_3538
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source nitrite 0.1mM remove 3,943,216 + Pf1N1B4_3535 0.82 +0.6 3,943,216 + Pf1N1B4_3535 0.82 +0.4 3,943,216 + Pf1N1B4_3535 0.82 -0.4 3,943,216 + Pf1N1B4_3535 0.82 -0.1 3,943,216 + -2.2 3,943,216 + +0.2 3,943,216 + Pf1N1B4_3535 0.82 -0.2 3,943,216 + Pf1N1B4_3535 0.82 -1.8 3,943,216 + Pf1N1B4_3535 0.82 +0.0 3,943,216 + Pf1N1B4_3535 0.82 +0.1 3,943,216 + Pf1N1B4_3535 0.82 -1.0 3,943,216 + Pf1N1B4_3535 0.82 -2.6 3,943,217 - Pf1N1B4_3535 0.82 -0.4 3,943,217 - Pf1N1B4_3535 0.82 +0.8 3,943,217 - Pf1N1B4_3535 0.82 -0.7 3,943,217 - Pf1N1B4_3535 0.82 -0.9 3,943,217 - Pf1N1B4_3535 0.82 -0.6 3,943,217 - Pf1N1B4_3535 0.82 -0.8 3,943,217 - Pf1N1B4_3535 0.82 +0.4 3,943,563 - -1.6 3,943,563 - -1.4 3,943,563 - +1.7 3,943,563 - +0.5 3,943,563 - -2.1 3,943,647 + +0.1 3,943,647 + -0.1 3,943,721 + -1.8 3,943,721 + -0.2 3,943,743 + Pf1N1B4_3536 0.11 +0.0 3,943,743 + Pf1N1B4_3536 0.11 -1.6 3,943,743 + Pf1N1B4_3536 0.11 +0.5 3,943,744 - Pf1N1B4_3536 0.12 +1.3 3,943,744 - Pf1N1B4_3536 0.12 -0.1 3,943,744 - Pf1N1B4_3536 0.12 -1.8 3,943,744 - Pf1N1B4_3536 0.12 +0.5 3,943,744 - Pf1N1B4_3536 0.12 -0.6 3,943,802 + Pf1N1B4_3536 0.32 +0.1 3,943,802 + Pf1N1B4_3536 0.32 +0.5 3,943,802 + Pf1N1B4_3536 0.32 -0.1 3,944,279 + Pf1N1B4_3537 0.32 -1.3 3,944,279 + Pf1N1B4_3537 0.32 +1.3 3,944,280 - Pf1N1B4_3537 0.32 -0.6 3,944,288 + Pf1N1B4_3537 0.33 -3.0 3,944,289 - Pf1N1B4_3537 0.33 +0.4 3,944,289 - Pf1N1B4_3537 0.33 -2.3 3,944,289 - Pf1N1B4_3537 0.33 -1.7 3,944,289 - Pf1N1B4_3537 0.33 -2.7 3,944,495 - Pf1N1B4_3537 0.56 -1.5 3,944,495 - Pf1N1B4_3537 0.56 -2.0 3,944,495 - Pf1N1B4_3537 0.56 +0.2 3,944,498 - Pf1N1B4_3537 0.57 +0.6 3,944,898 - -2.0 3,944,935 - +1.0 3,944,935 - +1.0 3,945,005 - +1.3 3,945,005 - -3.0 3,945,130 + +0.1 3,945,130 + -2.4 3,945,136 + -2.8 3,945,137 - -1.0 3,945,155 + +1.4 3,945,156 - +0.1 3,945,156 - -1.5 3,945,156 - +0.4 3,945,352 + Pf1N1B4_3538 0.23 +0.2 3,945,353 - Pf1N1B4_3538 0.23 -1.0 3,945,353 - +2.5 3,945,353 - Pf1N1B4_3538 0.23 -0.7 3,945,460 + Pf1N1B4_3538 0.37 -0.3 3,945,490 + Pf1N1B4_3538 0.41 +1.4 3,945,490 + Pf1N1B4_3538 0.41 +0.1
Or see this region's nucleotide sequence