Experiment: nitrogen source nitrite 0.1mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3358 and Pf1N1B4_3359 are separated by 32 nucleotides Pf1N1B4_3359 and Pf1N1B4_3360 are separated by 13 nucleotides Pf1N1B4_3360 and Pf1N1B4_3361 are separated by 125 nucleotides
Pf1N1B4_3358 - Permeases of the major facilitator superfamily, at 3,744,588 to 3,745,085
_3358
Pf1N1B4_3359 - Glutamine amidotransferase, class-II, at 3,745,118 to 3,745,894
_3359
Pf1N1B4_3360 - FIG00953701: hypothetical protein, at 3,745,908 to 3,746,450
_3360
Pf1N1B4_3361 - Outer membrane receptor proteins, likely involved in siderophore uptake @ TonB-dependent siderophore receptor, at 3,746,576 to 3,748,687
_3361
Position (kb)
3745
3746
3747 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 3745.098 kb on - strand at 3745.129 kb on - strand at 3745.238 kb on + strand at 3745.239 kb on - strand, within Pf1N1B4_3359 at 3745.239 kb on - strand, within Pf1N1B4_3359 at 3745.920 kb on + strand at 3745.920 kb on + strand at 3745.921 kb on - strand at 3745.921 kb on - strand at 3745.921 kb on - strand at 3745.990 kb on - strand, within Pf1N1B4_3360 at 3746.078 kb on + strand, within Pf1N1B4_3360 at 3746.078 kb on + strand, within Pf1N1B4_3360 at 3746.078 kb on + strand, within Pf1N1B4_3360 at 3746.078 kb on + strand, within Pf1N1B4_3360 at 3746.079 kb on - strand, within Pf1N1B4_3360 at 3746.211 kb on + strand, within Pf1N1B4_3360 at 3746.211 kb on + strand, within Pf1N1B4_3360 at 3746.211 kb on + strand, within Pf1N1B4_3360 at 3746.211 kb on + strand, within Pf1N1B4_3360 at 3746.211 kb on + strand, within Pf1N1B4_3360 at 3746.212 kb on - strand, within Pf1N1B4_3360 at 3746.277 kb on + strand, within Pf1N1B4_3360 at 3746.277 kb on + strand, within Pf1N1B4_3360 at 3746.448 kb on + strand at 3746.501 kb on + strand at 3746.534 kb on + strand at 3746.534 kb on + strand at 3746.534 kb on + strand at 3746.535 kb on - strand at 3746.535 kb on - strand at 3746.578 kb on - strand at 3746.668 kb on - strand at 3746.689 kb on - strand at 3746.689 kb on - strand at 3746.689 kb on - strand at 3746.689 kb on - strand at 3746.749 kb on + strand at 3746.749 kb on + strand at 3746.750 kb on - strand at 3746.750 kb on - strand at 3746.750 kb on - strand at 3746.750 kb on - strand at 3746.750 kb on - strand at 3746.752 kb on - strand at 3746.796 kb on + strand, within Pf1N1B4_3361 at 3746.832 kb on + strand, within Pf1N1B4_3361 at 3746.833 kb on - strand, within Pf1N1B4_3361 at 3746.847 kb on + strand, within Pf1N1B4_3361 at 3746.943 kb on + strand, within Pf1N1B4_3361 at 3746.943 kb on + strand, within Pf1N1B4_3361 at 3746.943 kb on + strand, within Pf1N1B4_3361 at 3746.944 kb on - strand, within Pf1N1B4_3361 at 3746.944 kb on - strand at 3746.944 kb on - strand, within Pf1N1B4_3361 at 3746.944 kb on - strand, within Pf1N1B4_3361 at 3746.944 kb on - strand at 3746.944 kb on - strand, within Pf1N1B4_3361 at 3747.055 kb on - strand, within Pf1N1B4_3361 at 3747.055 kb on - strand, within Pf1N1B4_3361 at 3747.055 kb on - strand, within Pf1N1B4_3361 at 3747.084 kb on + strand, within Pf1N1B4_3361 at 3747.084 kb on + strand, within Pf1N1B4_3361 at 3747.244 kb on - strand, within Pf1N1B4_3361 at 3747.244 kb on - strand, within Pf1N1B4_3361 at 3747.297 kb on + strand, within Pf1N1B4_3361 at 3747.297 kb on + strand, within Pf1N1B4_3361 at 3747.298 kb on - strand, within Pf1N1B4_3361 at 3747.312 kb on + strand, within Pf1N1B4_3361 at 3747.313 kb on - strand, within Pf1N1B4_3361 at 3747.370 kb on - strand, within Pf1N1B4_3361
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source nitrite 0.1mM remove 3,745,098 - +1.3 3,745,129 - -0.1 3,745,238 + +1.5 3,745,239 - Pf1N1B4_3359 0.16 +0.9 3,745,239 - Pf1N1B4_3359 0.16 -1.3 3,745,920 + +1.2 3,745,920 + +1.0 3,745,921 - +2.4 3,745,921 - -0.7 3,745,921 - +2.0 3,745,990 - Pf1N1B4_3360 0.15 +0.5 3,746,078 + Pf1N1B4_3360 0.31 +0.1 3,746,078 + Pf1N1B4_3360 0.31 +0.4 3,746,078 + Pf1N1B4_3360 0.31 +0.2 3,746,078 + Pf1N1B4_3360 0.31 +2.3 3,746,079 - Pf1N1B4_3360 0.31 +0.9 3,746,211 + Pf1N1B4_3360 0.56 +1.5 3,746,211 + Pf1N1B4_3360 0.56 +1.5 3,746,211 + Pf1N1B4_3360 0.56 +0.2 3,746,211 + Pf1N1B4_3360 0.56 +1.2 3,746,211 + Pf1N1B4_3360 0.56 -1.5 3,746,212 - Pf1N1B4_3360 0.56 -0.0 3,746,277 + Pf1N1B4_3360 0.68 +2.1 3,746,277 + Pf1N1B4_3360 0.68 -2.9 3,746,448 + -0.1 3,746,501 + -1.1 3,746,534 + +0.3 3,746,534 + -1.2 3,746,534 + +0.4 3,746,535 - -1.6 3,746,535 - -0.2 3,746,578 - -0.3 3,746,668 - -0.3 3,746,689 - -0.9 3,746,689 - +0.7 3,746,689 - -1.1 3,746,689 - +1.4 3,746,749 + -0.2 3,746,749 + +4.0 3,746,750 - +2.3 3,746,750 - +1.6 3,746,750 - +1.1 3,746,750 - +0.7 3,746,750 - +1.1 3,746,752 - +0.3 3,746,796 + Pf1N1B4_3361 0.10 -0.1 3,746,832 + Pf1N1B4_3361 0.12 +0.3 3,746,833 - Pf1N1B4_3361 0.12 +2.3 3,746,847 + Pf1N1B4_3361 0.13 -1.6 3,746,943 + Pf1N1B4_3361 0.17 -2.3 3,746,943 + Pf1N1B4_3361 0.17 +0.3 3,746,943 + Pf1N1B4_3361 0.17 -1.8 3,746,944 - Pf1N1B4_3361 0.17 -1.1 3,746,944 - +1.6 3,746,944 - Pf1N1B4_3361 0.17 -1.7 3,746,944 - Pf1N1B4_3361 0.17 +0.5 3,746,944 - -0.3 3,746,944 - Pf1N1B4_3361 0.17 -2.3 3,747,055 - Pf1N1B4_3361 0.23 +0.8 3,747,055 - Pf1N1B4_3361 0.23 +1.2 3,747,055 - Pf1N1B4_3361 0.23 +0.6 3,747,084 + Pf1N1B4_3361 0.24 +1.5 3,747,084 + Pf1N1B4_3361 0.24 +0.8 3,747,244 - Pf1N1B4_3361 0.32 +1.4 3,747,244 - Pf1N1B4_3361 0.32 -0.7 3,747,297 + Pf1N1B4_3361 0.34 -0.7 3,747,297 + Pf1N1B4_3361 0.34 -0.8 3,747,298 - Pf1N1B4_3361 0.34 +0.5 3,747,312 + Pf1N1B4_3361 0.35 +0.5 3,747,313 - Pf1N1B4_3361 0.35 +1.6 3,747,370 - Pf1N1B4_3361 0.38 -0.0
Or see this region's nucleotide sequence