Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_3360

Experiment: nitrogen source nitrite 0.1mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_3358 and Pf1N1B4_3359 are separated by 32 nucleotidesPf1N1B4_3359 and Pf1N1B4_3360 are separated by 13 nucleotidesPf1N1B4_3360 and Pf1N1B4_3361 are separated by 125 nucleotides Pf1N1B4_3358 - Permeases of the major facilitator superfamily, at 3,744,588 to 3,745,085 _3358 Pf1N1B4_3359 - Glutamine amidotransferase, class-II, at 3,745,118 to 3,745,894 _3359 Pf1N1B4_3360 - FIG00953701: hypothetical protein, at 3,745,908 to 3,746,450 _3360 Pf1N1B4_3361 - Outer membrane receptor proteins, likely involved in siderophore uptake @ TonB-dependent siderophore receptor, at 3,746,576 to 3,748,687 _3361 Position (kb) 3745 3746 3747Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3 4at 3745.098 kb on - strandat 3745.129 kb on - strandat 3745.238 kb on + strandat 3745.239 kb on - strand, within Pf1N1B4_3359at 3745.239 kb on - strand, within Pf1N1B4_3359at 3745.920 kb on + strandat 3745.920 kb on + strandat 3745.921 kb on - strandat 3745.921 kb on - strandat 3745.921 kb on - strandat 3745.990 kb on - strand, within Pf1N1B4_3360at 3746.078 kb on + strand, within Pf1N1B4_3360at 3746.078 kb on + strand, within Pf1N1B4_3360at 3746.078 kb on + strand, within Pf1N1B4_3360at 3746.078 kb on + strand, within Pf1N1B4_3360at 3746.079 kb on - strand, within Pf1N1B4_3360at 3746.211 kb on + strand, within Pf1N1B4_3360at 3746.211 kb on + strand, within Pf1N1B4_3360at 3746.211 kb on + strand, within Pf1N1B4_3360at 3746.211 kb on + strand, within Pf1N1B4_3360at 3746.211 kb on + strand, within Pf1N1B4_3360at 3746.212 kb on - strand, within Pf1N1B4_3360at 3746.277 kb on + strand, within Pf1N1B4_3360at 3746.277 kb on + strand, within Pf1N1B4_3360at 3746.448 kb on + strandat 3746.501 kb on + strandat 3746.534 kb on + strandat 3746.534 kb on + strandat 3746.534 kb on + strandat 3746.535 kb on - strandat 3746.535 kb on - strandat 3746.578 kb on - strandat 3746.668 kb on - strandat 3746.689 kb on - strandat 3746.689 kb on - strandat 3746.689 kb on - strandat 3746.689 kb on - strandat 3746.749 kb on + strandat 3746.749 kb on + strandat 3746.750 kb on - strandat 3746.750 kb on - strandat 3746.750 kb on - strandat 3746.750 kb on - strandat 3746.750 kb on - strandat 3746.752 kb on - strandat 3746.796 kb on + strand, within Pf1N1B4_3361at 3746.832 kb on + strand, within Pf1N1B4_3361at 3746.833 kb on - strand, within Pf1N1B4_3361at 3746.847 kb on + strand, within Pf1N1B4_3361at 3746.943 kb on + strand, within Pf1N1B4_3361at 3746.943 kb on + strand, within Pf1N1B4_3361at 3746.943 kb on + strand, within Pf1N1B4_3361at 3746.944 kb on - strand, within Pf1N1B4_3361at 3746.944 kb on - strandat 3746.944 kb on - strand, within Pf1N1B4_3361at 3746.944 kb on - strand, within Pf1N1B4_3361at 3746.944 kb on - strandat 3746.944 kb on - strand, within Pf1N1B4_3361at 3747.055 kb on - strand, within Pf1N1B4_3361at 3747.055 kb on - strand, within Pf1N1B4_3361at 3747.055 kb on - strand, within Pf1N1B4_3361at 3747.084 kb on + strand, within Pf1N1B4_3361at 3747.084 kb on + strand, within Pf1N1B4_3361at 3747.244 kb on - strand, within Pf1N1B4_3361at 3747.244 kb on - strand, within Pf1N1B4_3361at 3747.297 kb on + strand, within Pf1N1B4_3361at 3747.297 kb on + strand, within Pf1N1B4_3361at 3747.298 kb on - strand, within Pf1N1B4_3361at 3747.312 kb on + strand, within Pf1N1B4_3361at 3747.313 kb on - strand, within Pf1N1B4_3361at 3747.370 kb on - strand, within Pf1N1B4_3361

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Per-strain Table

Position Strand Gene LocusTag Fraction nitrogen source nitrite 0.1mM
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3,745,098 - +1.3
3,745,129 - -0.1
3,745,238 + +1.5
3,745,239 - Pf1N1B4_3359 0.16 +0.9
3,745,239 - Pf1N1B4_3359 0.16 -1.3
3,745,920 + +1.2
3,745,920 + +1.0
3,745,921 - +2.4
3,745,921 - -0.7
3,745,921 - +2.0
3,745,990 - Pf1N1B4_3360 0.15 +0.5
3,746,078 + Pf1N1B4_3360 0.31 +0.1
3,746,078 + Pf1N1B4_3360 0.31 +0.4
3,746,078 + Pf1N1B4_3360 0.31 +0.2
3,746,078 + Pf1N1B4_3360 0.31 +2.3
3,746,079 - Pf1N1B4_3360 0.31 +0.9
3,746,211 + Pf1N1B4_3360 0.56 +1.5
3,746,211 + Pf1N1B4_3360 0.56 +1.5
3,746,211 + Pf1N1B4_3360 0.56 +0.2
3,746,211 + Pf1N1B4_3360 0.56 +1.2
3,746,211 + Pf1N1B4_3360 0.56 -1.5
3,746,212 - Pf1N1B4_3360 0.56 -0.0
3,746,277 + Pf1N1B4_3360 0.68 +2.1
3,746,277 + Pf1N1B4_3360 0.68 -2.9
3,746,448 + -0.1
3,746,501 + -1.1
3,746,534 + +0.3
3,746,534 + -1.2
3,746,534 + +0.4
3,746,535 - -1.6
3,746,535 - -0.2
3,746,578 - -0.3
3,746,668 - -0.3
3,746,689 - -0.9
3,746,689 - +0.7
3,746,689 - -1.1
3,746,689 - +1.4
3,746,749 + -0.2
3,746,749 + +4.0
3,746,750 - +2.3
3,746,750 - +1.6
3,746,750 - +1.1
3,746,750 - +0.7
3,746,750 - +1.1
3,746,752 - +0.3
3,746,796 + Pf1N1B4_3361 0.10 -0.1
3,746,832 + Pf1N1B4_3361 0.12 +0.3
3,746,833 - Pf1N1B4_3361 0.12 +2.3
3,746,847 + Pf1N1B4_3361 0.13 -1.6
3,746,943 + Pf1N1B4_3361 0.17 -2.3
3,746,943 + Pf1N1B4_3361 0.17 +0.3
3,746,943 + Pf1N1B4_3361 0.17 -1.8
3,746,944 - Pf1N1B4_3361 0.17 -1.1
3,746,944 - +1.6
3,746,944 - Pf1N1B4_3361 0.17 -1.7
3,746,944 - Pf1N1B4_3361 0.17 +0.5
3,746,944 - -0.3
3,746,944 - Pf1N1B4_3361 0.17 -2.3
3,747,055 - Pf1N1B4_3361 0.23 +0.8
3,747,055 - Pf1N1B4_3361 0.23 +1.2
3,747,055 - Pf1N1B4_3361 0.23 +0.6
3,747,084 + Pf1N1B4_3361 0.24 +1.5
3,747,084 + Pf1N1B4_3361 0.24 +0.8
3,747,244 - Pf1N1B4_3361 0.32 +1.4
3,747,244 - Pf1N1B4_3361 0.32 -0.7
3,747,297 + Pf1N1B4_3361 0.34 -0.7
3,747,297 + Pf1N1B4_3361 0.34 -0.8
3,747,298 - Pf1N1B4_3361 0.34 +0.5
3,747,312 + Pf1N1B4_3361 0.35 +0.5
3,747,313 - Pf1N1B4_3361 0.35 +1.6
3,747,370 - Pf1N1B4_3361 0.38 -0.0

Or see this region's nucleotide sequence