Experiment: nitrogen source nitrite 0.1mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_248 and Pf1N1B4_249 are separated by 67 nucleotides Pf1N1B4_249 and Pf1N1B4_250 are separated by 181 nucleotides Pf1N1B4_250 and Pf1N1B4_251 are separated by 256 nucleotides
Pf1N1B4_248 - EAL-domain protein, at 304,330 to 305,493
_248
Pf1N1B4_249 - Deoxyguanosinetriphosphate triphosphohydrolase (EC 3.1.5.1), at 305,561 to 306,892
_249
Pf1N1B4_250 - response regulator, at 307,074 to 307,466
_250
Pf1N1B4_251 - DNA-binding response regulator, LuxR family, at 307,723 to 308,349
_251
Position (kb) 305
306
307 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 304.574 kb on + strand, within Pf1N1B4_248 at 304.575 kb on - strand, within Pf1N1B4_248 at 304.981 kb on + strand, within Pf1N1B4_248 at 304.986 kb on - strand, within Pf1N1B4_248 at 304.986 kb on - strand, within Pf1N1B4_248 at 304.986 kb on - strand, within Pf1N1B4_248 at 305.072 kb on - strand at 305.314 kb on - strand, within Pf1N1B4_248 at 305.338 kb on + strand at 305.339 kb on - strand, within Pf1N1B4_248 at 305.393 kb on - strand at 305.491 kb on + strand at 305.492 kb on - strand at 305.492 kb on - strand at 306.033 kb on + strand, within Pf1N1B4_249 at 306.033 kb on + strand, within Pf1N1B4_249 at 306.038 kb on - strand, within Pf1N1B4_249 at 306.038 kb on - strand at 306.038 kb on - strand, within Pf1N1B4_249 at 306.050 kb on + strand, within Pf1N1B4_249 at 306.084 kb on - strand, within Pf1N1B4_249 at 306.084 kb on - strand, within Pf1N1B4_249 at 306.084 kb on - strand, within Pf1N1B4_249 at 306.101 kb on + strand, within Pf1N1B4_249 at 306.101 kb on + strand, within Pf1N1B4_249 at 306.101 kb on + strand, within Pf1N1B4_249 at 306.111 kb on - strand, within Pf1N1B4_249 at 306.263 kb on + strand, within Pf1N1B4_249 at 306.264 kb on - strand, within Pf1N1B4_249 at 306.654 kb on - strand, within Pf1N1B4_249 at 306.745 kb on - strand, within Pf1N1B4_249 at 306.789 kb on + strand at 306.790 kb on - strand at 306.790 kb on - strand at 306.790 kb on - strand at 306.842 kb on + strand at 306.842 kb on + strand at 306.842 kb on + strand at 306.842 kb on + strand at 306.842 kb on + strand at 306.842 kb on + strand at 306.843 kb on - strand at 306.843 kb on - strand at 306.843 kb on - strand at 306.843 kb on - strand at 306.843 kb on - strand at 306.956 kb on + strand at 306.957 kb on - strand at 306.982 kb on - strand at 307.030 kb on + strand at 307.031 kb on - strand at 307.073 kb on + strand at 307.073 kb on + strand at 307.074 kb on - strand at 307.074 kb on - strand at 307.074 kb on - strand at 307.291 kb on - strand, within Pf1N1B4_250 at 307.325 kb on - strand, within Pf1N1B4_250 at 307.555 kb on - strand at 307.593 kb on + strand at 307.597 kb on + strand at 307.644 kb on + strand at 307.738 kb on - strand at 307.799 kb on - strand, within Pf1N1B4_251
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source nitrite 0.1mM remove 304,574 + Pf1N1B4_248 0.21 +0.8 304,575 - Pf1N1B4_248 0.21 +1.3 304,981 + Pf1N1B4_248 0.56 +0.6 304,986 - Pf1N1B4_248 0.56 -2.2 304,986 - Pf1N1B4_248 0.56 +0.1 304,986 - Pf1N1B4_248 0.56 +0.6 305,072 - +1.9 305,314 - Pf1N1B4_248 0.85 +1.5 305,338 + -1.3 305,339 - Pf1N1B4_248 0.87 +1.3 305,393 - +1.0 305,491 + +0.0 305,492 - +0.1 305,492 - +0.3 306,033 + Pf1N1B4_249 0.35 +0.2 306,033 + Pf1N1B4_249 0.35 +1.9 306,038 - Pf1N1B4_249 0.36 -0.9 306,038 - +0.9 306,038 - Pf1N1B4_249 0.36 +1.5 306,050 + Pf1N1B4_249 0.37 +0.1 306,084 - Pf1N1B4_249 0.39 +0.0 306,084 - Pf1N1B4_249 0.39 -3.2 306,084 - Pf1N1B4_249 0.39 +1.2 306,101 + Pf1N1B4_249 0.41 -0.1 306,101 + Pf1N1B4_249 0.41 +0.9 306,101 + Pf1N1B4_249 0.41 -2.3 306,111 - Pf1N1B4_249 0.41 +1.0 306,263 + Pf1N1B4_249 0.53 +1.3 306,264 - Pf1N1B4_249 0.53 +0.1 306,654 - Pf1N1B4_249 0.82 +1.6 306,745 - Pf1N1B4_249 0.89 +0.9 306,789 + +1.9 306,790 - +1.9 306,790 - +2.3 306,790 - +1.2 306,842 + +0.6 306,842 + -0.3 306,842 + -1.1 306,842 + -0.8 306,842 + +0.1 306,842 + -1.0 306,843 - -3.4 306,843 - +0.4 306,843 - -0.0 306,843 - +0.7 306,843 - +0.0 306,956 + -0.9 306,957 - +0.3 306,982 - +0.7 307,030 + -2.1 307,031 - -0.7 307,073 + +0.2 307,073 + +0.4 307,074 - -1.1 307,074 - -0.9 307,074 - -2.2 307,291 - Pf1N1B4_250 0.55 +0.3 307,325 - Pf1N1B4_250 0.64 -2.9 307,555 - -0.4 307,593 + +0.0 307,597 + +1.6 307,644 + +1.5 307,738 - -1.1 307,799 - Pf1N1B4_251 0.12 +0.2
Or see this region's nucleotide sequence