Experiment: nitrogen source nitrite 0.1mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_245 and Pf1N1B4_246 are separated by 1 nucleotides Pf1N1B4_246 and Pf1N1B4_247 overlap by 4 nucleotides Pf1N1B4_247 and Pf1N1B4_248 are separated by 123 nucleotides Pf1N1B4_248 and Pf1N1B4_249 are separated by 67 nucleotides
Pf1N1B4_245 - Probable transmembrane protein, at 303,166 to 303,480
_245
Pf1N1B4_246 - Probable transmembrane protein, at 303,482 to 303,871
_246
Pf1N1B4_247 - FIG00956101: hypothetical protein, at 303,868 to 304,206
_247
Pf1N1B4_248 - EAL-domain protein, at 304,330 to 305,493
_248
Pf1N1B4_249 - Deoxyguanosinetriphosphate triphosphohydrolase (EC 3.1.5.1), at 305,561 to 306,892
_249
Position (kb) 304
305
306 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 303.397 kb on + strand, within Pf1N1B4_245 at 303.397 kb on + strand, within Pf1N1B4_245 at 303.482 kb on - strand at 303.482 kb on - strand at 303.716 kb on + strand, within Pf1N1B4_246 at 303.752 kb on + strand, within Pf1N1B4_246 at 303.752 kb on + strand, within Pf1N1B4_246 at 303.752 kb on + strand, within Pf1N1B4_246 at 303.752 kb on + strand, within Pf1N1B4_246 at 303.752 kb on + strand, within Pf1N1B4_246 at 303.752 kb on + strand, within Pf1N1B4_246 at 303.752 kb on + strand, within Pf1N1B4_246 at 303.752 kb on + strand, within Pf1N1B4_246 at 303.752 kb on + strand, within Pf1N1B4_246 at 303.752 kb on + strand, within Pf1N1B4_246 at 303.753 kb on - strand, within Pf1N1B4_246 at 304.204 kb on + strand at 304.247 kb on + strand at 304.315 kb on + strand at 304.315 kb on + strand at 304.316 kb on - strand at 304.318 kb on + strand at 304.318 kb on + strand at 304.319 kb on - strand at 304.319 kb on - strand at 304.319 kb on - strand at 304.574 kb on + strand, within Pf1N1B4_248 at 304.575 kb on - strand, within Pf1N1B4_248 at 304.981 kb on + strand, within Pf1N1B4_248 at 304.986 kb on - strand, within Pf1N1B4_248 at 304.986 kb on - strand, within Pf1N1B4_248 at 304.986 kb on - strand, within Pf1N1B4_248 at 305.072 kb on - strand at 305.314 kb on - strand, within Pf1N1B4_248 at 305.338 kb on + strand at 305.339 kb on - strand, within Pf1N1B4_248 at 305.393 kb on - strand at 305.491 kb on + strand at 305.492 kb on - strand at 305.492 kb on - strand at 306.033 kb on + strand, within Pf1N1B4_249 at 306.033 kb on + strand, within Pf1N1B4_249 at 306.038 kb on - strand, within Pf1N1B4_249 at 306.038 kb on - strand at 306.038 kb on - strand, within Pf1N1B4_249 at 306.050 kb on + strand, within Pf1N1B4_249 at 306.084 kb on - strand, within Pf1N1B4_249 at 306.084 kb on - strand, within Pf1N1B4_249 at 306.084 kb on - strand, within Pf1N1B4_249 at 306.101 kb on + strand, within Pf1N1B4_249 at 306.101 kb on + strand, within Pf1N1B4_249 at 306.101 kb on + strand, within Pf1N1B4_249 at 306.111 kb on - strand, within Pf1N1B4_249 at 306.263 kb on + strand, within Pf1N1B4_249 at 306.264 kb on - strand, within Pf1N1B4_249
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source nitrite 0.1mM remove 303,397 + Pf1N1B4_245 0.73 -2.2 303,397 + Pf1N1B4_245 0.73 +1.1 303,482 - -0.2 303,482 - +0.7 303,716 + Pf1N1B4_246 0.60 -1.3 303,752 + Pf1N1B4_246 0.69 -0.4 303,752 + Pf1N1B4_246 0.69 -0.9 303,752 + Pf1N1B4_246 0.69 -0.0 303,752 + Pf1N1B4_246 0.69 -0.2 303,752 + Pf1N1B4_246 0.69 +0.7 303,752 + Pf1N1B4_246 0.69 -0.8 303,752 + Pf1N1B4_246 0.69 +0.9 303,752 + Pf1N1B4_246 0.69 +1.9 303,752 + Pf1N1B4_246 0.69 +1.3 303,752 + Pf1N1B4_246 0.69 -0.2 303,753 - Pf1N1B4_246 0.69 +0.2 304,204 + +0.2 304,247 + -1.0 304,315 + -0.9 304,315 + +0.2 304,316 - -0.1 304,318 + +0.5 304,318 + -0.0 304,319 - -2.1 304,319 - +0.7 304,319 - +1.9 304,574 + Pf1N1B4_248 0.21 +0.8 304,575 - Pf1N1B4_248 0.21 +1.3 304,981 + Pf1N1B4_248 0.56 +0.6 304,986 - Pf1N1B4_248 0.56 -2.2 304,986 - Pf1N1B4_248 0.56 +0.1 304,986 - Pf1N1B4_248 0.56 +0.6 305,072 - +1.9 305,314 - Pf1N1B4_248 0.85 +1.5 305,338 + -1.3 305,339 - Pf1N1B4_248 0.87 +1.3 305,393 - +1.0 305,491 + +0.0 305,492 - +0.1 305,492 - +0.3 306,033 + Pf1N1B4_249 0.35 +0.2 306,033 + Pf1N1B4_249 0.35 +1.9 306,038 - Pf1N1B4_249 0.36 -0.9 306,038 - +0.9 306,038 - Pf1N1B4_249 0.36 +1.5 306,050 + Pf1N1B4_249 0.37 +0.1 306,084 - Pf1N1B4_249 0.39 +0.0 306,084 - Pf1N1B4_249 0.39 -3.2 306,084 - Pf1N1B4_249 0.39 +1.2 306,101 + Pf1N1B4_249 0.41 -0.1 306,101 + Pf1N1B4_249 0.41 +0.9 306,101 + Pf1N1B4_249 0.41 -2.3 306,111 - Pf1N1B4_249 0.41 +1.0 306,263 + Pf1N1B4_249 0.53 +1.3 306,264 - Pf1N1B4_249 0.53 +0.1
Or see this region's nucleotide sequence