Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_228

Experiment: nitrogen source nitrite 0.1mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_226 and Pf1N1B4_227 are separated by 74 nucleotidesPf1N1B4_227 and Pf1N1B4_228 are separated by 74 nucleotidesPf1N1B4_228 and Pf1N1B4_229 are separated by 90 nucleotides Pf1N1B4_226 - Inhibitor of the KinA pathway to sporulation, predicted exonuclease, at 287,856 to 288,404 _226 Pf1N1B4_227 - FIG074102: hypothetical protein, at 288,479 to 288,763 _227 Pf1N1B4_228 - Flavodoxin reductases (ferredoxin-NADPH reductases) family 1, at 288,838 to 289,641 _228 Pf1N1B4_229 - Chemotaxis protein CheV (EC 2.7.3.-), at 289,732 to 290,667 _229 Position (kb) 288 289 290Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 288.163 kb on - strand, within Pf1N1B4_226at 288.362 kb on + strandat 288.363 kb on - strandat 288.440 kb on - strandat 288.484 kb on + strandat 288.484 kb on + strandat 288.498 kb on - strandat 288.740 kb on + strandat 288.740 kb on + strandat 288.740 kb on + strandat 288.740 kb on + strandat 288.741 kb on - strandat 288.839 kb on + strandat 288.840 kb on - strandat 289.072 kb on - strand, within Pf1N1B4_228at 289.072 kb on - strand, within Pf1N1B4_228at 289.083 kb on - strand, within Pf1N1B4_228at 289.661 kb on - strandat 289.675 kb on - strandat 289.675 kb on - strandat 289.677 kb on - strandat 289.722 kb on - strandat 289.741 kb on + strandat 289.741 kb on + strandat 289.742 kb on - strandat 289.742 kb on - strandat 289.742 kb on - strandat 289.742 kb on - strandat 289.771 kb on + strandat 289.772 kb on - strandat 289.880 kb on + strand, within Pf1N1B4_229at 289.880 kb on + strand, within Pf1N1B4_229at 289.880 kb on + strand, within Pf1N1B4_229at 289.881 kb on - strand, within Pf1N1B4_229at 289.881 kb on - strand, within Pf1N1B4_229at 289.881 kb on - strand, within Pf1N1B4_229at 289.881 kb on - strand, within Pf1N1B4_229at 290.107 kb on - strand, within Pf1N1B4_229at 290.107 kb on - strand, within Pf1N1B4_229at 290.193 kb on - strand, within Pf1N1B4_229at 290.193 kb on - strand, within Pf1N1B4_229at 290.273 kb on + strand, within Pf1N1B4_229at 290.274 kb on - strand, within Pf1N1B4_229at 290.274 kb on - strand, within Pf1N1B4_229at 290.378 kb on + strandat 290.392 kb on - strand, within Pf1N1B4_229at 290.626 kb on + strandat 290.626 kb on + strandat 290.626 kb on + strandat 290.627 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction nitrogen source nitrite 0.1mM
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288,163 - Pf1N1B4_226 0.56 +1.6
288,362 + -2.4
288,363 - +1.1
288,440 - -3.1
288,484 + +1.6
288,484 + +2.6
288,498 - +1.4
288,740 + -1.8
288,740 + -0.7
288,740 + +0.2
288,740 + -3.0
288,741 - +1.7
288,839 + +0.1
288,840 - +0.4
289,072 - Pf1N1B4_228 0.29 -0.1
289,072 - Pf1N1B4_228 0.29 -0.9
289,083 - Pf1N1B4_228 0.30 -2.5
289,661 - +0.6
289,675 - -1.1
289,675 - +0.4
289,677 - +0.2
289,722 - -1.2
289,741 + -1.7
289,741 + -0.1
289,742 - +1.9
289,742 - -1.7
289,742 - +1.9
289,742 - +0.2
289,771 + -0.9
289,772 - +3.2
289,880 + Pf1N1B4_229 0.16 -0.7
289,880 + Pf1N1B4_229 0.16 -0.3
289,880 + Pf1N1B4_229 0.16 -0.6
289,881 - Pf1N1B4_229 0.16 +0.1
289,881 - Pf1N1B4_229 0.16 -0.4
289,881 - Pf1N1B4_229 0.16 +0.8
289,881 - Pf1N1B4_229 0.16 +0.2
290,107 - Pf1N1B4_229 0.40 -1.7
290,107 - Pf1N1B4_229 0.40 +0.3
290,193 - Pf1N1B4_229 0.49 -3.4
290,193 - Pf1N1B4_229 0.49 -0.3
290,273 + Pf1N1B4_229 0.58 -2.0
290,274 - Pf1N1B4_229 0.58 -1.0
290,274 - Pf1N1B4_229 0.58 -2.6
290,378 + +0.2
290,392 - Pf1N1B4_229 0.71 +0.9
290,626 + +1.2
290,626 + +0.7
290,626 + +1.2
290,627 - +0.4

Or see this region's nucleotide sequence