Experiment: nitrogen source nitrite 0.1mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1495 and Pf1N1B4_1496 are separated by 169 nucleotides Pf1N1B4_1496 and Pf1N1B4_1497 are separated by 34 nucleotides
Pf1N1B4_1495 - Methyl-accepting chemotaxis protein I (serine chemoreceptor protein), at 1,724,741 to 1,726,351
_1495
Pf1N1B4_1496 - Na(+)/H(+) antiporter, at 1,726,521 to 1,728,263
_1496
Pf1N1B4_1497 - Potassium efflux system KefA protein / Small-conductance mechanosensitive channel, at 1,728,298 to 1,731,648
_1497
Position (kb)
1726
1727
1728
1729 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 1725.786 kb on + strand, within Pf1N1B4_1495 at 1725.787 kb on - strand, within Pf1N1B4_1495 at 1725.787 kb on - strand, within Pf1N1B4_1495 at 1725.787 kb on - strand, within Pf1N1B4_1495 at 1725.787 kb on - strand, within Pf1N1B4_1495 at 1726.096 kb on + strand, within Pf1N1B4_1495 at 1726.129 kb on + strand, within Pf1N1B4_1495 at 1726.129 kb on + strand, within Pf1N1B4_1495 at 1726.129 kb on + strand, within Pf1N1B4_1495 at 1726.129 kb on + strand, within Pf1N1B4_1495 at 1726.130 kb on - strand, within Pf1N1B4_1495 at 1726.130 kb on - strand, within Pf1N1B4_1495 at 1726.130 kb on - strand, within Pf1N1B4_1495 at 1726.130 kb on - strand at 1726.130 kb on - strand, within Pf1N1B4_1495 at 1726.130 kb on - strand, within Pf1N1B4_1495 at 1726.130 kb on - strand, within Pf1N1B4_1495 at 1726.130 kb on - strand, within Pf1N1B4_1495 at 1726.132 kb on + strand at 1726.132 kb on + strand, within Pf1N1B4_1495 at 1726.132 kb on + strand, within Pf1N1B4_1495 at 1726.132 kb on + strand, within Pf1N1B4_1495 at 1726.133 kb on - strand, within Pf1N1B4_1495 at 1726.133 kb on - strand, within Pf1N1B4_1495 at 1726.133 kb on - strand, within Pf1N1B4_1495 at 1726.342 kb on + strand at 1726.358 kb on - strand at 1726.383 kb on + strand at 1726.384 kb on - strand at 1726.454 kb on + strand at 1726.455 kb on - strand at 1726.455 kb on - strand at 1726.501 kb on - strand at 1726.501 kb on - strand at 1726.514 kb on - strand at 1726.570 kb on + strand at 1726.571 kb on - strand at 1726.571 kb on - strand at 1726.677 kb on - strand at 1726.689 kb on + strand at 1726.690 kb on - strand at 1726.690 kb on - strand at 1726.702 kb on - strand, within Pf1N1B4_1496 at 1726.871 kb on + strand, within Pf1N1B4_1496 at 1726.871 kb on + strand, within Pf1N1B4_1496 at 1726.871 kb on + strand, within Pf1N1B4_1496 at 1726.871 kb on + strand, within Pf1N1B4_1496 at 1727.040 kb on - strand, within Pf1N1B4_1496 at 1727.186 kb on + strand, within Pf1N1B4_1496 at 1727.265 kb on + strand, within Pf1N1B4_1496 at 1727.265 kb on + strand, within Pf1N1B4_1496 at 1727.607 kb on + strand, within Pf1N1B4_1496 at 1727.607 kb on + strand, within Pf1N1B4_1496 at 1727.608 kb on - strand, within Pf1N1B4_1496 at 1727.608 kb on - strand, within Pf1N1B4_1496 at 1727.608 kb on - strand, within Pf1N1B4_1496 at 1727.924 kb on - strand, within Pf1N1B4_1496 at 1728.161 kb on + strand at 1728.161 kb on + strand at 1728.211 kb on + strand at 1728.211 kb on + strand at 1728.211 kb on + strand at 1728.212 kb on - strand at 1728.261 kb on + strand at 1728.297 kb on + strand at 1728.298 kb on - strand at 1728.298 kb on - strand at 1728.298 kb on - strand at 1728.506 kb on - strand at 1728.625 kb on + strand at 1728.767 kb on + strand, within Pf1N1B4_1497 at 1729.145 kb on - strand, within Pf1N1B4_1497
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source nitrite 0.1mM remove 1,725,786 + Pf1N1B4_1495 0.65 -1.2 1,725,787 - Pf1N1B4_1495 0.65 -1.5 1,725,787 - Pf1N1B4_1495 0.65 -0.3 1,725,787 - Pf1N1B4_1495 0.65 +0.7 1,725,787 - Pf1N1B4_1495 0.65 -1.9 1,726,096 + Pf1N1B4_1495 0.84 +0.1 1,726,129 + Pf1N1B4_1495 0.86 -0.1 1,726,129 + Pf1N1B4_1495 0.86 -0.5 1,726,129 + Pf1N1B4_1495 0.86 -2.0 1,726,129 + Pf1N1B4_1495 0.86 -0.7 1,726,130 - Pf1N1B4_1495 0.86 -2.1 1,726,130 - Pf1N1B4_1495 0.86 -2.6 1,726,130 - Pf1N1B4_1495 0.86 +1.4 1,726,130 - +2.7 1,726,130 - Pf1N1B4_1495 0.86 +1.0 1,726,130 - Pf1N1B4_1495 0.86 +0.2 1,726,130 - Pf1N1B4_1495 0.86 +0.6 1,726,130 - Pf1N1B4_1495 0.86 -0.5 1,726,132 + +4.4 1,726,132 + Pf1N1B4_1495 0.86 -0.1 1,726,132 + Pf1N1B4_1495 0.86 -3.0 1,726,132 + Pf1N1B4_1495 0.86 -0.5 1,726,133 - Pf1N1B4_1495 0.86 -0.5 1,726,133 - Pf1N1B4_1495 0.86 +0.8 1,726,133 - Pf1N1B4_1495 0.86 +0.3 1,726,342 + -1.3 1,726,358 - +0.7 1,726,383 + +0.5 1,726,384 - +1.2 1,726,454 + +1.3 1,726,455 - -1.3 1,726,455 - -2.0 1,726,501 - -2.2 1,726,501 - +0.4 1,726,514 - +1.5 1,726,570 + +0.9 1,726,571 - -1.9 1,726,571 - -2.2 1,726,677 - -1.6 1,726,689 + +0.9 1,726,690 - +1.3 1,726,690 - +1.2 1,726,702 - Pf1N1B4_1496 0.10 +0.4 1,726,871 + Pf1N1B4_1496 0.20 +1.4 1,726,871 + Pf1N1B4_1496 0.20 +0.2 1,726,871 + Pf1N1B4_1496 0.20 +0.6 1,726,871 + Pf1N1B4_1496 0.20 +0.6 1,727,040 - Pf1N1B4_1496 0.30 +0.9 1,727,186 + Pf1N1B4_1496 0.38 +0.8 1,727,265 + Pf1N1B4_1496 0.43 +0.8 1,727,265 + Pf1N1B4_1496 0.43 +2.0 1,727,607 + Pf1N1B4_1496 0.62 -0.6 1,727,607 + Pf1N1B4_1496 0.62 -1.8 1,727,608 - Pf1N1B4_1496 0.62 -1.9 1,727,608 - Pf1N1B4_1496 0.62 +1.7 1,727,608 - Pf1N1B4_1496 0.62 +1.4 1,727,924 - Pf1N1B4_1496 0.80 +1.0 1,728,161 + +1.9 1,728,161 + +0.4 1,728,211 + -1.2 1,728,211 + -1.9 1,728,211 + +1.9 1,728,212 - -3.3 1,728,261 + -2.3 1,728,297 + +0.3 1,728,298 - -0.6 1,728,298 - -2.3 1,728,298 - -1.3 1,728,506 - +1.1 1,728,625 + +0.8 1,728,767 + Pf1N1B4_1497 0.14 -0.0 1,729,145 - Pf1N1B4_1497 0.25 +0.8
Or see this region's nucleotide sequence