Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_1496

Experiment: nitrogen source nitrite 0.1mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_1495 and Pf1N1B4_1496 are separated by 169 nucleotidesPf1N1B4_1496 and Pf1N1B4_1497 are separated by 34 nucleotides Pf1N1B4_1495 - Methyl-accepting chemotaxis protein I (serine chemoreceptor protein), at 1,724,741 to 1,726,351 _1495 Pf1N1B4_1496 - Na(+)/H(+) antiporter, at 1,726,521 to 1,728,263 _1496 Pf1N1B4_1497 - Potassium efflux system KefA protein / Small-conductance mechanosensitive channel, at 1,728,298 to 1,731,648 _1497 Position (kb) 1726 1727 1728 1729Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3 4at 1725.786 kb on + strand, within Pf1N1B4_1495at 1725.787 kb on - strand, within Pf1N1B4_1495at 1725.787 kb on - strand, within Pf1N1B4_1495at 1725.787 kb on - strand, within Pf1N1B4_1495at 1725.787 kb on - strand, within Pf1N1B4_1495at 1726.096 kb on + strand, within Pf1N1B4_1495at 1726.129 kb on + strand, within Pf1N1B4_1495at 1726.129 kb on + strand, within Pf1N1B4_1495at 1726.129 kb on + strand, within Pf1N1B4_1495at 1726.129 kb on + strand, within Pf1N1B4_1495at 1726.130 kb on - strand, within Pf1N1B4_1495at 1726.130 kb on - strand, within Pf1N1B4_1495at 1726.130 kb on - strand, within Pf1N1B4_1495at 1726.130 kb on - strandat 1726.130 kb on - strand, within Pf1N1B4_1495at 1726.130 kb on - strand, within Pf1N1B4_1495at 1726.130 kb on - strand, within Pf1N1B4_1495at 1726.130 kb on - strand, within Pf1N1B4_1495at 1726.132 kb on + strandat 1726.132 kb on + strand, within Pf1N1B4_1495at 1726.132 kb on + strand, within Pf1N1B4_1495at 1726.132 kb on + strand, within Pf1N1B4_1495at 1726.133 kb on - strand, within Pf1N1B4_1495at 1726.133 kb on - strand, within Pf1N1B4_1495at 1726.133 kb on - strand, within Pf1N1B4_1495at 1726.342 kb on + strandat 1726.358 kb on - strandat 1726.383 kb on + strandat 1726.384 kb on - strandat 1726.454 kb on + strandat 1726.455 kb on - strandat 1726.455 kb on - strandat 1726.501 kb on - strandat 1726.501 kb on - strandat 1726.514 kb on - strandat 1726.570 kb on + strandat 1726.571 kb on - strandat 1726.571 kb on - strandat 1726.677 kb on - strandat 1726.689 kb on + strandat 1726.690 kb on - strandat 1726.690 kb on - strandat 1726.702 kb on - strand, within Pf1N1B4_1496at 1726.871 kb on + strand, within Pf1N1B4_1496at 1726.871 kb on + strand, within Pf1N1B4_1496at 1726.871 kb on + strand, within Pf1N1B4_1496at 1726.871 kb on + strand, within Pf1N1B4_1496at 1727.040 kb on - strand, within Pf1N1B4_1496at 1727.186 kb on + strand, within Pf1N1B4_1496at 1727.265 kb on + strand, within Pf1N1B4_1496at 1727.265 kb on + strand, within Pf1N1B4_1496at 1727.607 kb on + strand, within Pf1N1B4_1496at 1727.607 kb on + strand, within Pf1N1B4_1496at 1727.608 kb on - strand, within Pf1N1B4_1496at 1727.608 kb on - strand, within Pf1N1B4_1496at 1727.608 kb on - strand, within Pf1N1B4_1496at 1727.924 kb on - strand, within Pf1N1B4_1496at 1728.161 kb on + strandat 1728.161 kb on + strandat 1728.211 kb on + strandat 1728.211 kb on + strandat 1728.211 kb on + strandat 1728.212 kb on - strandat 1728.261 kb on + strandat 1728.297 kb on + strandat 1728.298 kb on - strandat 1728.298 kb on - strandat 1728.298 kb on - strandat 1728.506 kb on - strandat 1728.625 kb on + strandat 1728.767 kb on + strand, within Pf1N1B4_1497at 1729.145 kb on - strand, within Pf1N1B4_1497

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Per-strain Table

Position Strand Gene LocusTag Fraction nitrogen source nitrite 0.1mM
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1,725,786 + Pf1N1B4_1495 0.65 -1.2
1,725,787 - Pf1N1B4_1495 0.65 -1.5
1,725,787 - Pf1N1B4_1495 0.65 -0.3
1,725,787 - Pf1N1B4_1495 0.65 +0.7
1,725,787 - Pf1N1B4_1495 0.65 -1.9
1,726,096 + Pf1N1B4_1495 0.84 +0.1
1,726,129 + Pf1N1B4_1495 0.86 -0.1
1,726,129 + Pf1N1B4_1495 0.86 -0.5
1,726,129 + Pf1N1B4_1495 0.86 -2.0
1,726,129 + Pf1N1B4_1495 0.86 -0.7
1,726,130 - Pf1N1B4_1495 0.86 -2.1
1,726,130 - Pf1N1B4_1495 0.86 -2.6
1,726,130 - Pf1N1B4_1495 0.86 +1.4
1,726,130 - +2.7
1,726,130 - Pf1N1B4_1495 0.86 +1.0
1,726,130 - Pf1N1B4_1495 0.86 +0.2
1,726,130 - Pf1N1B4_1495 0.86 +0.6
1,726,130 - Pf1N1B4_1495 0.86 -0.5
1,726,132 + +4.4
1,726,132 + Pf1N1B4_1495 0.86 -0.1
1,726,132 + Pf1N1B4_1495 0.86 -3.0
1,726,132 + Pf1N1B4_1495 0.86 -0.5
1,726,133 - Pf1N1B4_1495 0.86 -0.5
1,726,133 - Pf1N1B4_1495 0.86 +0.8
1,726,133 - Pf1N1B4_1495 0.86 +0.3
1,726,342 + -1.3
1,726,358 - +0.7
1,726,383 + +0.5
1,726,384 - +1.2
1,726,454 + +1.3
1,726,455 - -1.3
1,726,455 - -2.0
1,726,501 - -2.2
1,726,501 - +0.4
1,726,514 - +1.5
1,726,570 + +0.9
1,726,571 - -1.9
1,726,571 - -2.2
1,726,677 - -1.6
1,726,689 + +0.9
1,726,690 - +1.3
1,726,690 - +1.2
1,726,702 - Pf1N1B4_1496 0.10 +0.4
1,726,871 + Pf1N1B4_1496 0.20 +1.4
1,726,871 + Pf1N1B4_1496 0.20 +0.2
1,726,871 + Pf1N1B4_1496 0.20 +0.6
1,726,871 + Pf1N1B4_1496 0.20 +0.6
1,727,040 - Pf1N1B4_1496 0.30 +0.9
1,727,186 + Pf1N1B4_1496 0.38 +0.8
1,727,265 + Pf1N1B4_1496 0.43 +0.8
1,727,265 + Pf1N1B4_1496 0.43 +2.0
1,727,607 + Pf1N1B4_1496 0.62 -0.6
1,727,607 + Pf1N1B4_1496 0.62 -1.8
1,727,608 - Pf1N1B4_1496 0.62 -1.9
1,727,608 - Pf1N1B4_1496 0.62 +1.7
1,727,608 - Pf1N1B4_1496 0.62 +1.4
1,727,924 - Pf1N1B4_1496 0.80 +1.0
1,728,161 + +1.9
1,728,161 + +0.4
1,728,211 + -1.2
1,728,211 + -1.9
1,728,211 + +1.9
1,728,212 - -3.3
1,728,261 + -2.3
1,728,297 + +0.3
1,728,298 - -0.6
1,728,298 - -2.3
1,728,298 - -1.3
1,728,506 - +1.1
1,728,625 + +0.8
1,728,767 + Pf1N1B4_1497 0.14 -0.0
1,729,145 - Pf1N1B4_1497 0.25 +0.8

Or see this region's nucleotide sequence