Experiment: nitrogen source nitrite 0.1mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_117 and Pf1N1B4_118 are separated by 40 nucleotides Pf1N1B4_118 and Pf1N1B4_119 are separated by 277 nucleotides Pf1N1B4_119 and Pf1N1B4_120 are separated by 92 nucleotides
Pf1N1B4_117 - Iron siderophore sensor protein, at 123,814 to 124,788
_117
Pf1N1B4_118 - Sigma-70 factor FpvI (ECF subfamily), controling pyoverdin biosynthesis @ FIG006045: Sigma factor, ECF subfamily, at 124,829 to 125,329
_118
Pf1N1B4_119 - Putative haemolysin/cytolysin secreted via TPS pathway, at 125,607 to 130,118
_119
Pf1N1B4_120 - Channel-forming transporter/cytolysins activator of TpsB family, at 130,211 to 131,938
_120
Position (kb)
125
126
127
128
129
130
131 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 124.839 kb on - strand at 124.839 kb on - strand at 124.839 kb on - strand at 124.883 kb on - strand at 124.883 kb on - strand, within Pf1N1B4_118 at 124.883 kb on - strand, within Pf1N1B4_118 at 124.884 kb on + strand, within Pf1N1B4_118 at 124.884 kb on + strand, within Pf1N1B4_118 at 124.884 kb on + strand, within Pf1N1B4_118 at 124.885 kb on - strand, within Pf1N1B4_118 at 124.885 kb on - strand, within Pf1N1B4_118 at 124.885 kb on - strand at 125.406 kb on + strand at 125.406 kb on + strand at 125.407 kb on - strand at 125.407 kb on - strand at 125.437 kb on + strand at 125.441 kb on + strand at 125.441 kb on + strand at 125.441 kb on + strand at 125.442 kb on - strand at 125.456 kb on + strand at 125.456 kb on + strand at 125.456 kb on + strand at 125.457 kb on - strand at 125.478 kb on + strand at 125.484 kb on + strand at 125.608 kb on + strand at 125.608 kb on + strand at 125.608 kb on + strand at 125.608 kb on + strand at 125.608 kb on + strand at 125.609 kb on - strand at 125.609 kb on - strand at 125.609 kb on - strand at 125.609 kb on - strand at 125.609 kb on - strand at 125.720 kb on + strand at 125.974 kb on + strand at 125.974 kb on + strand at 126.238 kb on + strand, within Pf1N1B4_119 at 126.239 kb on - strand, within Pf1N1B4_119 at 126.397 kb on + strand, within Pf1N1B4_119 at 126.789 kb on - strand at 126.789 kb on - strand, within Pf1N1B4_119 at 127.107 kb on - strand, within Pf1N1B4_119 at 127.565 kb on + strand, within Pf1N1B4_119 at 127.565 kb on + strand at 127.565 kb on + strand, within Pf1N1B4_119 at 127.566 kb on - strand, within Pf1N1B4_119 at 127.566 kb on - strand, within Pf1N1B4_119 at 127.566 kb on - strand, within Pf1N1B4_119 at 127.566 kb on - strand at 127.566 kb on - strand, within Pf1N1B4_119 at 127.680 kb on + strand, within Pf1N1B4_119 at 127.680 kb on + strand, within Pf1N1B4_119 at 127.680 kb on + strand, within Pf1N1B4_119 at 127.680 kb on + strand, within Pf1N1B4_119 at 127.680 kb on + strand, within Pf1N1B4_119 at 127.681 kb on - strand, within Pf1N1B4_119 at 127.801 kb on + strand at 127.801 kb on + strand, within Pf1N1B4_119 at 127.801 kb on + strand, within Pf1N1B4_119 at 127.802 kb on - strand, within Pf1N1B4_119 at 127.804 kb on + strand, within Pf1N1B4_119 at 128.083 kb on + strand, within Pf1N1B4_119 at 129.286 kb on - strand, within Pf1N1B4_119 at 129.703 kb on + strand at 129.703 kb on + strand at 129.704 kb on - strand at 129.704 kb on - strand at 129.704 kb on - strand at 129.704 kb on - strand at 129.704 kb on - strand at 129.892 kb on + strand at 129.893 kb on - strand at 129.893 kb on - strand at 130.134 kb on - strand at 130.291 kb on - strand at 130.456 kb on + strand at 130.569 kb on + strand, within Pf1N1B4_120 at 130.650 kb on + strand, within Pf1N1B4_120 at 130.734 kb on + strand, within Pf1N1B4_120 at 130.843 kb on - strand, within Pf1N1B4_120 at 131.020 kb on - strand, within Pf1N1B4_120 at 131.047 kb on - strand, within Pf1N1B4_120 at 131.050 kb on - strand, within Pf1N1B4_120
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source nitrite 0.1mM remove 124,839 - -1.4 124,839 - -0.0 124,839 - +0.3 124,883 - -0.9 124,883 - Pf1N1B4_118 0.11 +1.0 124,883 - Pf1N1B4_118 0.11 +1.0 124,884 + Pf1N1B4_118 0.11 -0.1 124,884 + Pf1N1B4_118 0.11 -0.7 124,884 + Pf1N1B4_118 0.11 +2.2 124,885 - Pf1N1B4_118 0.11 +0.4 124,885 - Pf1N1B4_118 0.11 -2.0 124,885 - +0.3 125,406 + -0.7 125,406 + +1.6 125,407 - +0.4 125,407 - +0.1 125,437 + -0.9 125,441 + +0.5 125,441 + +1.0 125,441 + +0.6 125,442 - -2.0 125,456 + -1.9 125,456 + -3.3 125,456 + +1.3 125,457 - -0.3 125,478 + -2.2 125,484 + -1.8 125,608 + +0.3 125,608 + -2.0 125,608 + +1.7 125,608 + +0.3 125,608 + -1.8 125,609 - -0.0 125,609 - +0.5 125,609 - -1.0 125,609 - +0.6 125,609 - -1.6 125,720 + +0.5 125,974 + +1.0 125,974 + +0.0 126,238 + Pf1N1B4_119 0.14 +1.1 126,239 - Pf1N1B4_119 0.14 +0.7 126,397 + Pf1N1B4_119 0.18 +1.0 126,789 - +2.1 126,789 - Pf1N1B4_119 0.26 +2.7 127,107 - Pf1N1B4_119 0.33 -1.4 127,565 + Pf1N1B4_119 0.43 +0.7 127,565 + +0.3 127,565 + Pf1N1B4_119 0.43 +1.0 127,566 - Pf1N1B4_119 0.43 +0.1 127,566 - Pf1N1B4_119 0.43 -1.9 127,566 - Pf1N1B4_119 0.43 +1.1 127,566 - +2.7 127,566 - Pf1N1B4_119 0.43 -0.3 127,680 + Pf1N1B4_119 0.46 +2.7 127,680 + Pf1N1B4_119 0.46 +0.4 127,680 + Pf1N1B4_119 0.46 +2.0 127,680 + Pf1N1B4_119 0.46 -2.0 127,680 + Pf1N1B4_119 0.46 +1.2 127,681 - Pf1N1B4_119 0.46 +0.7 127,801 + -0.7 127,801 + Pf1N1B4_119 0.49 +1.4 127,801 + Pf1N1B4_119 0.49 +0.6 127,802 - Pf1N1B4_119 0.49 -2.2 127,804 + Pf1N1B4_119 0.49 -0.2 128,083 + Pf1N1B4_119 0.55 +1.1 129,286 - Pf1N1B4_119 0.82 +2.2 129,703 + -0.7 129,703 + -1.7 129,704 - +1.4 129,704 - -1.9 129,704 - -1.6 129,704 - +0.3 129,704 - -1.5 129,892 + -0.3 129,893 - -1.9 129,893 - -0.7 130,134 - +1.1 130,291 - +1.4 130,456 + -1.6 130,569 + Pf1N1B4_120 0.21 +0.7 130,650 + Pf1N1B4_120 0.25 +0.1 130,734 + Pf1N1B4_120 0.30 +0.6 130,843 - Pf1N1B4_120 0.37 +1.3 131,020 - Pf1N1B4_120 0.47 -0.7 131,047 - Pf1N1B4_120 0.48 +2.0 131,050 - Pf1N1B4_120 0.49 +0.1
Or see this region's nucleotide sequence