Experiment: KB with Methyl-2-Hydroxy-Phenylproprionate 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_4561 and Psyr_RNA66 are separated by 46 nucleotides Psyr_RNA66 and Psyr_RNA67 are separated by 165 nucleotides Psyr_RNA67 and Psyr_RNA68 are separated by 26 nucleotides Psyr_RNA68 and Psyr_RNA69 are separated by 24 nucleotides Psyr_RNA69 and Psyr_4562 are separated by 142 nucleotides Psyr_4562 and Psyr_4563 are separated by 7 nucleotides Psyr_4563 and Psyr_4564 overlap by 11 nucleotides
Psyr_4561: Psyr_4561 - protein translocase subunit secE/sec61 gamma, at 5,419,660 to 5,420,028
_4561
Psyr_RNA66: Psyr_RNA66 - tRNA-Trp, at 5,420,075 to 5,420,150
_RNA66
Psyr_RNA67: Psyr_RNA67 - tRNA-Thr, at 5,420,316 to 5,420,391
_RNA67
Psyr_RNA68: Psyr_RNA68 - tRNA-Gly, at 5,420,418 to 5,420,491
_RNA68
Psyr_RNA69: Psyr_RNA69 - tRNA-Tyr, at 5,420,516 to 5,420,600
_RNA69
Psyr_4562: Psyr_4562 - conserved domain protein, at 5,420,743 to 5,421,171
_4562
Psyr_4563: Psyr_4563 - Bvg accessory factor, at 5,421,179 to 5,421,928
_4563
Psyr_4564: Psyr_4564 - Biotin--acetyl-CoA-carboxylase ligase, at 5,421,918 to 5,422,925
_4564
Position (kb)
5420
5421
5422 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 5420.061 kb on - strand at 5420.061 kb on - strand at 5420.061 kb on - strand at 5420.061 kb on - strand at 5420.061 kb on - strand at 5420.061 kb on - strand at 5420.061 kb on - strand at 5420.061 kb on - strand at 5420.061 kb on - strand at 5420.067 kb on - strand at 5420.067 kb on - strand at 5420.207 kb on - strand at 5420.278 kb on - strand at 5420.293 kb on - strand at 5420.293 kb on - strand at 5420.293 kb on - strand at 5420.301 kb on - strand at 5420.670 kb on - strand at 5420.670 kb on - strand at 5420.686 kb on - strand at 5420.736 kb on - strand at 5420.736 kb on - strand at 5420.736 kb on - strand at 5420.736 kb on - strand at 5420.736 kb on - strand at 5420.740 kb on - strand at 5420.740 kb on - strand at 5420.777 kb on - strand at 5420.777 kb on - strand at 5420.871 kb on - strand, within Psyr_4562 at 5420.981 kb on + strand, within Psyr_4562 at 5420.981 kb on + strand, within Psyr_4562 at 5420.982 kb on - strand, within Psyr_4562 at 5421.057 kb on + strand, within Psyr_4562 at 5421.058 kb on - strand, within Psyr_4562 at 5421.060 kb on + strand, within Psyr_4562 at 5421.060 kb on + strand, within Psyr_4562 at 5421.061 kb on - strand, within Psyr_4562 at 5421.125 kb on + strand, within Psyr_4562 at 5421.125 kb on + strand, within Psyr_4562 at 5421.125 kb on + strand, within Psyr_4562 at 5421.125 kb on + strand, within Psyr_4562 at 5421.125 kb on + strand, within Psyr_4562 at 5421.126 kb on - strand, within Psyr_4562 at 5421.128 kb on + strand, within Psyr_4562 at 5421.128 kb on + strand, within Psyr_4562 at 5421.128 kb on + strand, within Psyr_4562 at 5421.129 kb on - strand at 5421.138 kb on + strand at 5421.138 kb on + strand at 5421.160 kb on + strand at 5421.160 kb on + strand at 5421.161 kb on - strand at 5421.172 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Methyl-2-Hydroxy-Phenylproprionate 10 mM remove 5,420,061 - -0.1 5,420,061 - +0.8 5,420,061 - +1.1 5,420,061 - +1.9 5,420,061 - -0.0 5,420,061 - -0.1 5,420,061 - -1.3 5,420,061 - +0.8 5,420,061 - -0.4 5,420,067 - -0.4 5,420,067 - -0.7 5,420,207 - -1.1 5,420,278 - -0.9 5,420,293 - +0.2 5,420,293 - +0.4 5,420,293 - -0.3 5,420,301 - +0.0 5,420,670 - +0.3 5,420,670 - -0.4 5,420,686 - +0.1 5,420,736 - -0.1 5,420,736 - -0.3 5,420,736 - -0.1 5,420,736 - -0.7 5,420,736 - -1.8 5,420,740 - -1.1 5,420,740 - +0.9 5,420,777 - +1.1 5,420,777 - +1.0 5,420,871 - Psyr_4562 0.30 -0.3 5,420,981 + Psyr_4562 0.55 +0.5 5,420,981 + Psyr_4562 0.55 -0.5 5,420,982 - Psyr_4562 0.56 -1.0 5,421,057 + Psyr_4562 0.73 -2.6 5,421,058 - Psyr_4562 0.73 -0.6 5,421,060 + Psyr_4562 0.74 -1.5 5,421,060 + Psyr_4562 0.74 -0.2 5,421,061 - Psyr_4562 0.74 -0.2 5,421,125 + Psyr_4562 0.89 -1.4 5,421,125 + Psyr_4562 0.89 -0.2 5,421,125 + Psyr_4562 0.89 -0.7 5,421,125 + Psyr_4562 0.89 -0.9 5,421,125 + Psyr_4562 0.89 -0.2 5,421,126 - Psyr_4562 0.89 -0.7 5,421,128 + Psyr_4562 0.90 +2.1 5,421,128 + Psyr_4562 0.90 -0.8 5,421,128 + Psyr_4562 0.90 -1.9 5,421,129 - +0.2 5,421,138 + -2.2 5,421,138 + -1.0 5,421,160 + -0.7 5,421,160 + +1.1 5,421,161 - -0.4 5,421,172 - +1.3
Or see this region's nucleotide sequence