Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_717

Experiment: nitrogen source nitrite 0.5mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_716 and Pf1N1B4_717 are separated by 27 nucleotidesPf1N1B4_717 and Pf1N1B4_718 are separated by 14 nucleotides Pf1N1B4_716 - Probable thiol oxidoreductase with 2 cytochrome c heme-binding sites, at 816,248 to 817,675 _716 Pf1N1B4_717 - Iron-regulated protein A precursor, at 817,703 to 818,767 _717 Pf1N1B4_718 - outer membrane component of uptake system, probably for ferrous iron (from data), at 818,782 to 819,882 _718 Position (kb) 817 818 819Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 816.740 kb on - strand, within Pf1N1B4_716at 817.093 kb on + strand, within Pf1N1B4_716at 817.229 kb on + strand, within Pf1N1B4_716at 817.229 kb on + strand, within Pf1N1B4_716at 817.229 kb on + strand, within Pf1N1B4_716at 817.231 kb on + strand, within Pf1N1B4_716at 817.398 kb on - strand, within Pf1N1B4_716at 817.673 kb on + strandat 817.676 kb on + strandat 817.677 kb on - strandat 817.823 kb on + strand, within Pf1N1B4_717at 817.824 kb on - strand, within Pf1N1B4_717at 818.101 kb on - strand, within Pf1N1B4_717at 818.129 kb on + strand, within Pf1N1B4_717at 818.129 kb on + strand, within Pf1N1B4_717at 818.129 kb on + strandat 818.129 kb on + strand, within Pf1N1B4_717at 818.129 kb on + strand, within Pf1N1B4_717at 818.129 kb on + strandat 818.129 kb on + strand, within Pf1N1B4_717at 818.129 kb on + strand, within Pf1N1B4_717at 818.129 kb on + strand, within Pf1N1B4_717at 818.129 kb on + strand, within Pf1N1B4_717at 818.130 kb on - strand, within Pf1N1B4_717at 818.130 kb on - strand, within Pf1N1B4_717at 818.130 kb on - strand, within Pf1N1B4_717at 818.130 kb on - strand, within Pf1N1B4_717at 818.130 kb on - strand, within Pf1N1B4_717at 818.130 kb on - strand, within Pf1N1B4_717at 818.130 kb on - strand, within Pf1N1B4_717at 818.130 kb on - strand, within Pf1N1B4_717at 818.183 kb on + strand, within Pf1N1B4_717at 818.183 kb on + strand, within Pf1N1B4_717at 818.183 kb on + strand, within Pf1N1B4_717at 818.183 kb on + strand, within Pf1N1B4_717at 818.184 kb on - strand, within Pf1N1B4_717at 818.184 kb on - strand, within Pf1N1B4_717at 818.184 kb on - strand, within Pf1N1B4_717at 818.580 kb on - strand, within Pf1N1B4_717at 818.672 kb on + strandat 818.672 kb on + strandat 818.672 kb on + strandat 818.672 kb on + strandat 818.918 kb on - strand, within Pf1N1B4_718at 819.011 kb on + strand, within Pf1N1B4_718at 819.050 kb on - strand, within Pf1N1B4_718at 819.115 kb on + strand, within Pf1N1B4_718at 819.115 kb on + strand, within Pf1N1B4_718at 819.154 kb on + strand, within Pf1N1B4_718at 819.155 kb on - strand, within Pf1N1B4_718at 819.205 kb on + strand, within Pf1N1B4_718at 819.205 kb on + strand, within Pf1N1B4_718at 819.206 kb on - strand, within Pf1N1B4_718at 819.206 kb on - strand, within Pf1N1B4_718at 819.207 kb on + strand, within Pf1N1B4_718at 819.207 kb on + strand, within Pf1N1B4_718at 819.207 kb on + strand, within Pf1N1B4_718at 819.207 kb on + strand, within Pf1N1B4_718at 819.207 kb on + strand, within Pf1N1B4_718at 819.207 kb on + strand, within Pf1N1B4_718at 819.207 kb on + strand, within Pf1N1B4_718at 819.208 kb on - strand, within Pf1N1B4_718at 819.208 kb on - strand, within Pf1N1B4_718at 819.208 kb on - strand, within Pf1N1B4_718at 819.208 kb on - strand, within Pf1N1B4_718at 819.386 kb on + strand, within Pf1N1B4_718at 819.604 kb on + strand, within Pf1N1B4_718at 819.604 kb on + strand, within Pf1N1B4_718at 819.605 kb on - strand, within Pf1N1B4_718at 819.678 kb on + strand, within Pf1N1B4_718at 819.679 kb on - strand, within Pf1N1B4_718at 819.679 kb on - strand, within Pf1N1B4_718at 819.704 kb on + strand, within Pf1N1B4_718at 819.705 kb on - strand, within Pf1N1B4_718

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Per-strain Table

Position Strand Gene LocusTag Fraction nitrogen source nitrite 0.5mM
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816,740 - Pf1N1B4_716 0.34 +0.2
817,093 + Pf1N1B4_716 0.59 -3.3
817,229 + Pf1N1B4_716 0.69 -1.0
817,229 + Pf1N1B4_716 0.69 -1.1
817,229 + Pf1N1B4_716 0.69 -1.5
817,231 + Pf1N1B4_716 0.69 -1.4
817,398 - Pf1N1B4_716 0.81 +0.1
817,673 + +0.9
817,676 + +0.9
817,677 - -0.2
817,823 + Pf1N1B4_717 0.11 -1.5
817,824 - Pf1N1B4_717 0.11 -0.4
818,101 - Pf1N1B4_717 0.37 -0.7
818,129 + Pf1N1B4_717 0.40 -1.3
818,129 + Pf1N1B4_717 0.40 -0.8
818,129 + -1.2
818,129 + Pf1N1B4_717 0.40 -0.9
818,129 + Pf1N1B4_717 0.40 +0.1
818,129 + +0.3
818,129 + Pf1N1B4_717 0.40 +0.1
818,129 + Pf1N1B4_717 0.40 -2.1
818,129 + Pf1N1B4_717 0.40 +1.2
818,129 + Pf1N1B4_717 0.40 -2.4
818,130 - Pf1N1B4_717 0.40 -1.9
818,130 - Pf1N1B4_717 0.40 -3.1
818,130 - Pf1N1B4_717 0.40 -0.8
818,130 - Pf1N1B4_717 0.40 -0.1
818,130 - Pf1N1B4_717 0.40 -2.8
818,130 - Pf1N1B4_717 0.40 -1.4
818,130 - Pf1N1B4_717 0.40 -1.7
818,130 - Pf1N1B4_717 0.40 -2.3
818,183 + Pf1N1B4_717 0.45 -1.0
818,183 + Pf1N1B4_717 0.45 -0.6
818,183 + Pf1N1B4_717 0.45 -0.8
818,183 + Pf1N1B4_717 0.45 -3.2
818,184 - Pf1N1B4_717 0.45 -0.1
818,184 - Pf1N1B4_717 0.45 -1.5
818,184 - Pf1N1B4_717 0.45 +0.6
818,580 - Pf1N1B4_717 0.82 -1.4
818,672 + +0.0
818,672 + -2.2
818,672 + +1.2
818,672 + -2.2
818,918 - Pf1N1B4_718 0.12 +0.5
819,011 + Pf1N1B4_718 0.21 -1.4
819,050 - Pf1N1B4_718 0.24 -2.6
819,115 + Pf1N1B4_718 0.30 -1.0
819,115 + Pf1N1B4_718 0.30 +0.2
819,154 + Pf1N1B4_718 0.34 +1.2
819,155 - Pf1N1B4_718 0.34 -0.0
819,205 + Pf1N1B4_718 0.38 -2.4
819,205 + Pf1N1B4_718 0.38 -1.3
819,206 - Pf1N1B4_718 0.39 -0.4
819,206 - Pf1N1B4_718 0.39 +0.1
819,207 + Pf1N1B4_718 0.39 -1.2
819,207 + Pf1N1B4_718 0.39 +0.0
819,207 + Pf1N1B4_718 0.39 -2.4
819,207 + Pf1N1B4_718 0.39 -1.7
819,207 + Pf1N1B4_718 0.39 -0.9
819,207 + Pf1N1B4_718 0.39 +0.2
819,207 + Pf1N1B4_718 0.39 -1.0
819,208 - Pf1N1B4_718 0.39 -1.7
819,208 - Pf1N1B4_718 0.39 -1.1
819,208 - Pf1N1B4_718 0.39 -0.7
819,208 - Pf1N1B4_718 0.39 +0.6
819,386 + Pf1N1B4_718 0.55 +1.9
819,604 + Pf1N1B4_718 0.75 -0.7
819,604 + Pf1N1B4_718 0.75 -1.6
819,605 - Pf1N1B4_718 0.75 -3.0
819,678 + Pf1N1B4_718 0.81 -1.8
819,679 - Pf1N1B4_718 0.81 -0.2
819,679 - Pf1N1B4_718 0.81 -2.2
819,704 + Pf1N1B4_718 0.84 -0.8
819,705 - Pf1N1B4_718 0.84 -1.6

Or see this region's nucleotide sequence