Experiment: nitrogen source nitrite 0.5mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_695 and Pf1N1B4_696 overlap by 4 nucleotides Pf1N1B4_696 and Pf1N1B4_697 are separated by 159 nucleotides
Pf1N1B4_695 - Aldehyde dehydrogenase (EC 1.2.1.3), at 793,533 to 794,981
_695
Pf1N1B4_696 - Acetolactate synthase, large subunit (EC 2.2.1.6), at 794,978 to 796,615
_696
Pf1N1B4_697 - Glycine cleavage system transcriptional activator, at 796,775 to 797,656
_697
Position (kb)
794
795
796
797 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4
5 at 794.196 kb on + strand, within Pf1N1B4_695 at 794.450 kb on + strand, within Pf1N1B4_695 at 794.457 kb on + strand, within Pf1N1B4_695 at 794.641 kb on + strand at 794.642 kb on - strand, within Pf1N1B4_695 at 794.645 kb on - strand, within Pf1N1B4_695 at 794.645 kb on - strand, within Pf1N1B4_695 at 794.950 kb on + strand at 794.950 kb on + strand at 794.950 kb on + strand at 794.951 kb on - strand at 794.951 kb on - strand at 794.951 kb on - strand at 794.966 kb on + strand at 795.141 kb on + strand at 795.141 kb on + strand at 795.141 kb on + strand at 795.141 kb on + strand at 795.141 kb on + strand at 795.141 kb on + strand at 795.141 kb on + strand at 795.142 kb on - strand, within Pf1N1B4_696 at 795.142 kb on - strand, within Pf1N1B4_696 at 795.183 kb on + strand, within Pf1N1B4_696 at 795.183 kb on + strand, within Pf1N1B4_696 at 795.183 kb on + strand, within Pf1N1B4_696 at 795.183 kb on + strand, within Pf1N1B4_696 at 795.183 kb on + strand, within Pf1N1B4_696 at 795.183 kb on + strand, within Pf1N1B4_696 at 795.184 kb on - strand, within Pf1N1B4_696 at 795.184 kb on - strand, within Pf1N1B4_696 at 795.184 kb on - strand, within Pf1N1B4_696 at 795.184 kb on - strand, within Pf1N1B4_696 at 795.184 kb on - strand, within Pf1N1B4_696 at 795.184 kb on - strand, within Pf1N1B4_696 at 795.202 kb on - strand, within Pf1N1B4_696 at 795.453 kb on + strand, within Pf1N1B4_696 at 795.701 kb on - strand, within Pf1N1B4_696 at 796.356 kb on + strand, within Pf1N1B4_696 at 796.356 kb on + strand, within Pf1N1B4_696 at 796.583 kb on + strand at 796.595 kb on + strand at 796.595 kb on + strand at 796.595 kb on + strand at 796.595 kb on + strand at 796.595 kb on + strand at 796.595 kb on + strand at 796.596 kb on - strand at 796.596 kb on - strand at 796.596 kb on - strand at 796.663 kb on + strand at 796.663 kb on + strand at 796.664 kb on - strand at 796.664 kb on - strand at 796.664 kb on - strand at 796.729 kb on + strand at 796.739 kb on + strand at 796.740 kb on - strand at 796.740 kb on - strand at 796.742 kb on + strand at 796.743 kb on - strand at 796.743 kb on - strand at 796.761 kb on + strand at 796.762 kb on - strand at 796.762 kb on - strand at 796.762 kb on - strand at 796.762 kb on - strand at 796.919 kb on + strand, within Pf1N1B4_697 at 797.204 kb on + strand, within Pf1N1B4_697 at 797.204 kb on + strand, within Pf1N1B4_697 at 797.204 kb on + strand, within Pf1N1B4_697 at 797.204 kb on + strand, within Pf1N1B4_697 at 797.204 kb on + strand, within Pf1N1B4_697 at 797.204 kb on + strand, within Pf1N1B4_697 at 797.204 kb on + strand, within Pf1N1B4_697 at 797.204 kb on + strand, within Pf1N1B4_697 at 797.204 kb on + strand, within Pf1N1B4_697 at 797.204 kb on + strand, within Pf1N1B4_697 at 797.205 kb on - strand, within Pf1N1B4_697 at 797.567 kb on + strand, within Pf1N1B4_697 at 797.570 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source nitrite 0.5mM remove 794,196 + Pf1N1B4_695 0.46 +0.7 794,450 + Pf1N1B4_695 0.63 +1.3 794,457 + Pf1N1B4_695 0.64 -1.3 794,641 + +1.9 794,642 - Pf1N1B4_695 0.77 +1.6 794,645 - Pf1N1B4_695 0.77 +2.5 794,645 - Pf1N1B4_695 0.77 +3.2 794,950 + -0.8 794,950 + +0.8 794,950 + +0.9 794,951 - +2.8 794,951 - +1.8 794,951 - +2.9 794,966 + +0.6 795,141 + +1.0 795,141 + +0.1 795,141 + -1.2 795,141 + -1.1 795,141 + +1.2 795,141 + +0.3 795,141 + +0.2 795,142 - Pf1N1B4_696 0.10 +2.5 795,142 - Pf1N1B4_696 0.10 +1.5 795,183 + Pf1N1B4_696 0.13 +0.3 795,183 + Pf1N1B4_696 0.13 +1.0 795,183 + Pf1N1B4_696 0.13 +0.7 795,183 + Pf1N1B4_696 0.13 +1.8 795,183 + Pf1N1B4_696 0.13 +0.7 795,183 + Pf1N1B4_696 0.13 +0.1 795,184 - Pf1N1B4_696 0.13 +2.3 795,184 - Pf1N1B4_696 0.13 +2.2 795,184 - Pf1N1B4_696 0.13 +2.6 795,184 - Pf1N1B4_696 0.13 +1.8 795,184 - Pf1N1B4_696 0.13 +1.9 795,184 - Pf1N1B4_696 0.13 +2.5 795,202 - Pf1N1B4_696 0.14 +2.3 795,453 + Pf1N1B4_696 0.29 -1.9 795,701 - Pf1N1B4_696 0.44 +2.9 796,356 + Pf1N1B4_696 0.84 -1.3 796,356 + Pf1N1B4_696 0.84 +0.0 796,583 + +0.3 796,595 + +0.7 796,595 + -1.2 796,595 + -0.0 796,595 + -0.3 796,595 + -1.2 796,595 + +1.4 796,596 - +1.2 796,596 - +1.6 796,596 - +0.3 796,663 + -2.4 796,663 + +1.1 796,664 - +2.3 796,664 - +3.7 796,664 - +5.0 796,729 + +0.6 796,739 + +2.3 796,740 - -0.4 796,740 - -0.1 796,742 + -0.6 796,743 - +0.4 796,743 - +0.9 796,761 + -0.8 796,762 - +0.8 796,762 - +1.4 796,762 - +0.4 796,762 - -0.6 796,919 + Pf1N1B4_697 0.16 -0.3 797,204 + Pf1N1B4_697 0.49 +0.4 797,204 + Pf1N1B4_697 0.49 -0.1 797,204 + Pf1N1B4_697 0.49 +0.6 797,204 + Pf1N1B4_697 0.49 -0.4 797,204 + Pf1N1B4_697 0.49 +0.5 797,204 + Pf1N1B4_697 0.49 +0.1 797,204 + Pf1N1B4_697 0.49 +0.2 797,204 + Pf1N1B4_697 0.49 -0.7 797,204 + Pf1N1B4_697 0.49 -2.0 797,204 + Pf1N1B4_697 0.49 +2.1 797,205 - Pf1N1B4_697 0.49 +0.7 797,567 + Pf1N1B4_697 0.90 +0.3 797,570 + +0.7
Or see this region's nucleotide sequence