Experiment: nitrogen source nitrite 0.5mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_598 and Pf1N1B4_599 overlap by 11 nucleotides Pf1N1B4_599 and Pf1N1B4_600 are separated by 177 nucleotides Pf1N1B4_600 and Pf1N1B4_601 overlap by 4 nucleotides
Pf1N1B4_598 - Integral membrane sensor signal transduction histidine kinase (EC 2.7.13.3), glucose catabolism cluster, at 690,581 to 692,050
_598
Pf1N1B4_599 - DNA-binding response regulator GltR, controls specific porins for the entry of glucose, at 692,040 to 692,771
_599
Pf1N1B4_600 - Glucokinase (EC 2.7.1.2), at 692,949 to 693,908
_600
Pf1N1B4_601 - Phosphogluconate dehydratase (EC 4.2.1.12), at 693,905 to 695,731
_601
Position (kb) 692
693
694 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 692.081 kb on - strand at 692.458 kb on + strand, within Pf1N1B4_599 at 692.701 kb on + strand at 692.701 kb on + strand at 692.701 kb on + strand at 692.701 kb on + strand at 692.701 kb on + strand at 692.702 kb on - strand at 692.702 kb on - strand at 692.702 kb on - strand at 692.702 kb on - strand at 692.702 kb on - strand at 692.722 kb on - strand at 692.722 kb on - strand at 692.807 kb on + strand at 692.807 kb on + strand at 692.807 kb on + strand at 692.807 kb on + strand at 692.807 kb on + strand at 692.808 kb on - strand at 692.808 kb on - strand at 692.814 kb on + strand at 692.814 kb on + strand at 692.828 kb on - strand at 692.828 kb on - strand at 692.844 kb on + strand at 692.844 kb on + strand at 692.845 kb on - strand at 692.845 kb on - strand at 693.040 kb on + strand at 693.133 kb on + strand, within Pf1N1B4_600 at 693.133 kb on + strand at 693.133 kb on + strand at 693.133 kb on + strand, within Pf1N1B4_600 at 693.133 kb on + strand at 693.133 kb on + strand, within Pf1N1B4_600 at 693.133 kb on + strand, within Pf1N1B4_600 at 693.134 kb on - strand, within Pf1N1B4_600 at 693.311 kb on - strand, within Pf1N1B4_600 at 693.375 kb on + strand, within Pf1N1B4_600 at 693.375 kb on + strand, within Pf1N1B4_600 at 693.375 kb on + strand, within Pf1N1B4_600 at 693.376 kb on - strand, within Pf1N1B4_600 at 693.376 kb on - strand, within Pf1N1B4_600 at 693.376 kb on - strand, within Pf1N1B4_600 at 693.376 kb on - strand, within Pf1N1B4_600 at 693.376 kb on - strand, within Pf1N1B4_600 at 693.376 kb on - strand, within Pf1N1B4_600 at 693.376 kb on - strand, within Pf1N1B4_600 at 693.376 kb on - strand, within Pf1N1B4_600 at 693.376 kb on - strand, within Pf1N1B4_600 at 693.376 kb on - strand, within Pf1N1B4_600 at 693.376 kb on - strand, within Pf1N1B4_600 at 693.376 kb on - strand, within Pf1N1B4_600 at 693.376 kb on - strand, within Pf1N1B4_600 at 693.376 kb on - strand, within Pf1N1B4_600 at 693.775 kb on + strand, within Pf1N1B4_600 at 693.911 kb on - strand at 693.911 kb on - strand at 693.911 kb on - strand at 693.911 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source nitrite 0.5mM remove 692,081 - -0.5 692,458 + Pf1N1B4_599 0.57 -0.8 692,701 + -0.7 692,701 + +0.8 692,701 + -0.7 692,701 + -0.1 692,701 + -0.3 692,702 - -0.1 692,702 - -0.5 692,702 - -0.2 692,702 - -0.6 692,702 - +1.0 692,722 - -0.7 692,722 - -0.0 692,807 + +0.3 692,807 + +0.5 692,807 + -0.8 692,807 + -0.3 692,807 + +0.2 692,808 - -0.5 692,808 - -0.6 692,814 + -0.6 692,814 + -0.1 692,828 - +0.6 692,828 - -0.0 692,844 + -0.2 692,844 + -1.2 692,845 - -1.1 692,845 - +0.4 693,040 + -1.5 693,133 + Pf1N1B4_600 0.19 -0.6 693,133 + -1.9 693,133 + -1.2 693,133 + Pf1N1B4_600 0.19 -0.4 693,133 + +0.3 693,133 + Pf1N1B4_600 0.19 -1.0 693,133 + Pf1N1B4_600 0.19 +0.1 693,134 - Pf1N1B4_600 0.19 -1.3 693,311 - Pf1N1B4_600 0.38 -1.5 693,375 + Pf1N1B4_600 0.44 -1.0 693,375 + Pf1N1B4_600 0.44 -0.6 693,375 + Pf1N1B4_600 0.44 -0.8 693,376 - Pf1N1B4_600 0.44 -1.2 693,376 - Pf1N1B4_600 0.44 -1.5 693,376 - Pf1N1B4_600 0.44 -1.7 693,376 - Pf1N1B4_600 0.44 -0.2 693,376 - Pf1N1B4_600 0.44 -1.3 693,376 - Pf1N1B4_600 0.44 +0.3 693,376 - Pf1N1B4_600 0.44 -1.2 693,376 - Pf1N1B4_600 0.44 -1.4 693,376 - Pf1N1B4_600 0.44 +1.3 693,376 - Pf1N1B4_600 0.44 -2.2 693,376 - Pf1N1B4_600 0.44 -2.1 693,376 - Pf1N1B4_600 0.44 -0.9 693,376 - Pf1N1B4_600 0.44 -3.9 693,376 - Pf1N1B4_600 0.44 -2.3 693,775 + Pf1N1B4_600 0.86 -1.3 693,911 - -2.2 693,911 - -1.9 693,911 - -3.9 693,911 - +0.4
Or see this region's nucleotide sequence