Experiment: nitrogen source nitrite 0.5mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5726 and Pf1N1B4_5727 are separated by 15 nucleotides Pf1N1B4_5727 and Pf1N1B4_5728 are separated by 5 nucleotides Pf1N1B4_5728 and Pf1N1B4_5729 are separated by 32 nucleotides
Pf1N1B4_5726 - Ferredoxin, at 165,339 to 166,667
_5726
Pf1N1B4_5727 - Ribonucleotide reductase of class III (anaerobic), activating protein (EC 1.97.1.4), at 166,683 to 167,369
_5727
Pf1N1B4_5728 - hypothetical protein, at 167,375 to 167,542
_5728
Pf1N1B4_5729 - Ribonucleotide reductase of class III (anaerobic), large subunit (EC 1.17.4.2), at 167,575 to 169,590
_5729
Position (kb) 166
167
168 Strain fitness (log2 ratio) -2
-1
0
1 at 165.699 kb on + strand, within Pf1N1B4_5726 at 166.054 kb on + strand, within Pf1N1B4_5726 at 166.374 kb on + strand at 166.511 kb on - strand, within Pf1N1B4_5726 at 166.511 kb on - strand, within Pf1N1B4_5726 at 166.906 kb on + strand, within Pf1N1B4_5727 at 167.781 kb on - strand, within Pf1N1B4_5729 at 167.781 kb on - strand, within Pf1N1B4_5729 at 167.781 kb on - strand at 167.781 kb on - strand, within Pf1N1B4_5729 at 167.891 kb on + strand, within Pf1N1B4_5729 at 167.891 kb on + strand, within Pf1N1B4_5729 at 167.891 kb on + strand, within Pf1N1B4_5729 at 167.894 kb on + strand, within Pf1N1B4_5729 at 167.999 kb on + strand at 168.094 kb on - strand, within Pf1N1B4_5729 at 168.194 kb on + strand, within Pf1N1B4_5729 at 168.194 kb on + strand, within Pf1N1B4_5729 at 168.320 kb on + strand, within Pf1N1B4_5729 at 168.320 kb on + strand, within Pf1N1B4_5729
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source nitrite 0.5mM remove 165,699 + Pf1N1B4_5726 0.27 +0.3 166,054 + Pf1N1B4_5726 0.54 -1.4 166,374 + -0.9 166,511 - Pf1N1B4_5726 0.88 -0.1 166,511 - Pf1N1B4_5726 0.88 -1.0 166,906 + Pf1N1B4_5727 0.32 +0.1 167,781 - Pf1N1B4_5729 0.10 +0.5 167,781 - Pf1N1B4_5729 0.10 -0.2 167,781 - -1.4 167,781 - Pf1N1B4_5729 0.10 -1.7 167,891 + Pf1N1B4_5729 0.16 +1.0 167,891 + Pf1N1B4_5729 0.16 -1.7 167,891 + Pf1N1B4_5729 0.16 +0.3 167,894 + Pf1N1B4_5729 0.16 -0.8 167,999 + +0.8 168,094 - Pf1N1B4_5729 0.26 -0.5 168,194 + Pf1N1B4_5729 0.31 -0.3 168,194 + Pf1N1B4_5729 0.31 +0.2 168,320 + Pf1N1B4_5729 0.37 -2.3 168,320 + Pf1N1B4_5729 0.37 +1.6
Or see this region's nucleotide sequence