Experiment: nitrogen source nitrite 0.5mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5663 and Pf1N1B4_5664 are separated by 191 nucleotides Pf1N1B4_5664 and Pf1N1B4_5665 are separated by 291 nucleotides Pf1N1B4_5665 and Pf1N1B4_5666 overlap by 17 nucleotides Pf1N1B4_5666 and Pf1N1B4_5667 are separated by 44 nucleotides
Pf1N1B4_5663 - Periplasmic sugar-binding domain protein, at 97,139 to 98,149
_5663
Pf1N1B4_5664 - hypothetical protein, at 98,341 to 98,454
_5664
Pf1N1B4_5665 - Sulfite reductase [NADPH] hemoprotein beta-component (EC 1.8.1.2), at 98,746 to 100,404
_5665
Pf1N1B4_5666 - Oxidoreductase probably involved in sulfite reduction, at 100,388 to 100,882
_5666
Pf1N1B4_5667 - hypothetical protein, at 100,927 to 104,862
_5667
Position (kb) 98
99
100
101 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 97.789 kb on - strand, within Pf1N1B4_5663 at 97.789 kb on - strand, within Pf1N1B4_5663 at 97.789 kb on - strand, within Pf1N1B4_5663 at 97.837 kb on - strand, within Pf1N1B4_5663 at 97.950 kb on + strand, within Pf1N1B4_5663 at 97.950 kb on + strand, within Pf1N1B4_5663 at 98.103 kb on + strand at 98.192 kb on + strand at 98.208 kb on + strand at 98.209 kb on - strand at 98.209 kb on - strand at 98.260 kb on - strand at 98.260 kb on - strand at 98.342 kb on + strand at 98.343 kb on - strand at 98.609 kb on - strand at 98.622 kb on + strand at 98.623 kb on - strand at 98.623 kb on - strand at 98.709 kb on + strand at 98.710 kb on - strand at 98.710 kb on - strand at 98.743 kb on + strand at 98.743 kb on + strand at 98.743 kb on + strand at 98.743 kb on + strand at 98.743 kb on + strand at 98.743 kb on + strand at 98.743 kb on + strand at 98.743 kb on + strand at 98.743 kb on + strand at 98.744 kb on - strand at 98.744 kb on - strand at 98.744 kb on - strand at 98.744 kb on - strand at 98.744 kb on - strand at 98.744 kb on - strand at 98.744 kb on - strand at 98.744 kb on - strand at 98.744 kb on - strand at 98.745 kb on + strand at 98.745 kb on + strand at 98.745 kb on + strand at 98.745 kb on + strand at 98.745 kb on + strand at 98.745 kb on + strand at 98.745 kb on + strand at 98.745 kb on + strand at 98.746 kb on - strand at 98.746 kb on - strand at 98.746 kb on - strand at 98.746 kb on - strand at 98.746 kb on - strand at 98.746 kb on - strand at 98.746 kb on - strand at 98.746 kb on - strand at 98.746 kb on - strand at 98.746 kb on - strand at 98.746 kb on - strand at 98.746 kb on - strand at 98.746 kb on - strand at 98.746 kb on - strand at 98.746 kb on - strand at 98.749 kb on + strand at 98.749 kb on + strand at 98.749 kb on + strand at 98.750 kb on - strand at 98.750 kb on - strand at 98.750 kb on - strand at 98.750 kb on - strand at 98.750 kb on - strand at 98.750 kb on - strand at 98.750 kb on - strand at 98.750 kb on - strand at 98.753 kb on + strand at 98.753 kb on + strand at 98.753 kb on + strand at 98.754 kb on - strand at 98.754 kb on - strand at 98.754 kb on - strand at 98.754 kb on - strand at 98.754 kb on - strand at 98.755 kb on + strand at 98.756 kb on - strand at 98.756 kb on - strand at 98.764 kb on + strand at 98.881 kb on + strand at 98.881 kb on + strand at 98.882 kb on - strand at 98.882 kb on - strand at 98.882 kb on - strand at 98.882 kb on - strand at 99.238 kb on - strand, within Pf1N1B4_5665 at 99.430 kb on + strand, within Pf1N1B4_5665 at 99.430 kb on + strand, within Pf1N1B4_5665 at 99.430 kb on + strand, within Pf1N1B4_5665 at 99.431 kb on - strand, within Pf1N1B4_5665 at 99.455 kb on - strand, within Pf1N1B4_5665 at 99.484 kb on + strand, within Pf1N1B4_5665 at 99.484 kb on + strand at 99.485 kb on - strand, within Pf1N1B4_5665 at 99.485 kb on - strand, within Pf1N1B4_5665 at 99.745 kb on + strand, within Pf1N1B4_5665 at 100.075 kb on + strand, within Pf1N1B4_5665 at 100.076 kb on - strand, within Pf1N1B4_5665 at 100.192 kb on + strand, within Pf1N1B4_5665 at 100.192 kb on + strand, within Pf1N1B4_5665 at 100.192 kb on + strand, within Pf1N1B4_5665 at 100.306 kb on + strand at 100.306 kb on + strand at 100.306 kb on + strand at 100.306 kb on + strand at 100.306 kb on + strand at 100.306 kb on + strand at 100.348 kb on + strand at 100.348 kb on + strand at 100.348 kb on + strand at 100.348 kb on + strand at 100.348 kb on + strand at 100.349 kb on - strand at 100.388 kb on - strand at 100.399 kb on - strand at 100.399 kb on - strand at 100.402 kb on + strand at 100.402 kb on + strand at 100.402 kb on + strand at 100.403 kb on - strand at 100.522 kb on + strand, within Pf1N1B4_5666 at 100.655 kb on + strand, within Pf1N1B4_5666 at 100.655 kb on + strand, within Pf1N1B4_5666 at 100.655 kb on + strand, within Pf1N1B4_5666 at 100.742 kb on + strand, within Pf1N1B4_5666 at 100.742 kb on + strand, within Pf1N1B4_5666 at 100.742 kb on + strand, within Pf1N1B4_5666 at 100.796 kb on + strand, within Pf1N1B4_5666 at 100.835 kb on + strand at 100.835 kb on + strand at 100.928 kb on + strand at 100.929 kb on - strand at 101.136 kb on + strand at 101.136 kb on + strand at 101.136 kb on + strand at 101.137 kb on - strand at 101.137 kb on - strand at 101.139 kb on - strand at 101.139 kb on - strand at 101.139 kb on - strand at 101.270 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source nitrite 0.5mM remove 97,789 - Pf1N1B4_5663 0.64 -0.1 97,789 - Pf1N1B4_5663 0.64 +0.5 97,789 - Pf1N1B4_5663 0.64 -0.9 97,837 - Pf1N1B4_5663 0.69 -1.4 97,950 + Pf1N1B4_5663 0.80 +0.7 97,950 + Pf1N1B4_5663 0.80 +0.1 98,103 + +1.6 98,192 + -1.5 98,208 + +0.9 98,209 - -0.4 98,209 - +0.3 98,260 - -0.4 98,260 - -2.0 98,342 + -0.0 98,343 - -1.5 98,609 - -1.7 98,622 + +0.1 98,623 - +0.3 98,623 - -0.0 98,709 + -0.9 98,710 - -3.7 98,710 - +0.6 98,743 + +0.6 98,743 + +0.4 98,743 + -1.1 98,743 + -1.5 98,743 + -0.7 98,743 + -1.4 98,743 + -2.2 98,743 + -1.8 98,743 + -2.9 98,744 - -2.6 98,744 - -2.0 98,744 - -1.5 98,744 - -3.5 98,744 - -1.4 98,744 - -2.9 98,744 - -1.9 98,744 - -2.2 98,744 - -1.7 98,745 + -1.4 98,745 + -0.8 98,745 + -0.0 98,745 + -1.5 98,745 + -2.2 98,745 + +0.4 98,745 + -2.7 98,745 + -0.4 98,746 - -0.8 98,746 - -1.8 98,746 - +0.2 98,746 - -1.4 98,746 - -1.3 98,746 - -1.7 98,746 - -2.2 98,746 - -1.9 98,746 - -1.7 98,746 - -1.3 98,746 - -1.4 98,746 - -3.3 98,746 - -3.2 98,746 - -0.9 98,746 - -2.3 98,749 + +0.6 98,749 + -2.0 98,749 + -2.2 98,750 - +0.4 98,750 - -0.2 98,750 - -2.7 98,750 - -2.2 98,750 - -2.3 98,750 - -0.9 98,750 - +0.8 98,750 - +0.3 98,753 + -2.8 98,753 + -2.5 98,753 + -1.4 98,754 - -1.7 98,754 - -0.6 98,754 - -3.0 98,754 - +0.6 98,754 - -1.0 98,755 + -4.9 98,756 - -1.9 98,756 - +0.5 98,764 + -1.7 98,881 + -3.1 98,881 + -1.6 98,882 - -0.4 98,882 - +0.7 98,882 - -0.8 98,882 - -3.5 99,238 - Pf1N1B4_5665 0.30 -3.7 99,430 + Pf1N1B4_5665 0.41 -1.3 99,430 + Pf1N1B4_5665 0.41 -1.5 99,430 + Pf1N1B4_5665 0.41 -3.1 99,431 - Pf1N1B4_5665 0.41 -2.6 99,455 - Pf1N1B4_5665 0.43 -1.6 99,484 + Pf1N1B4_5665 0.44 -1.1 99,484 + -1.9 99,485 - Pf1N1B4_5665 0.45 -2.8 99,485 - Pf1N1B4_5665 0.45 -1.7 99,745 + Pf1N1B4_5665 0.60 -0.9 100,075 + Pf1N1B4_5665 0.80 +1.0 100,076 - Pf1N1B4_5665 0.80 -1.0 100,192 + Pf1N1B4_5665 0.87 -1.9 100,192 + Pf1N1B4_5665 0.87 -2.1 100,192 + Pf1N1B4_5665 0.87 -3.5 100,306 + -2.1 100,306 + -1.3 100,306 + -3.2 100,306 + -1.8 100,306 + -1.6 100,306 + -1.4 100,348 + -2.5 100,348 + -3.4 100,348 + +0.8 100,348 + -1.0 100,348 + -3.0 100,349 - -3.0 100,388 - +0.6 100,399 - +0.9 100,399 - +0.6 100,402 + -2.0 100,402 + -1.8 100,402 + -0.3 100,403 - -2.0 100,522 + Pf1N1B4_5666 0.27 -1.4 100,655 + Pf1N1B4_5666 0.54 -1.7 100,655 + Pf1N1B4_5666 0.54 -3.8 100,655 + Pf1N1B4_5666 0.54 -2.0 100,742 + Pf1N1B4_5666 0.72 -4.1 100,742 + Pf1N1B4_5666 0.72 -1.9 100,742 + Pf1N1B4_5666 0.72 +1.3 100,796 + Pf1N1B4_5666 0.82 -1.7 100,835 + -1.0 100,835 + -0.2 100,928 + -0.9 100,929 - +1.7 101,136 + -0.3 101,136 + +0.6 101,136 + +1.5 101,137 - -3.1 101,137 - +0.7 101,139 - +1.6 101,139 - -2.0 101,139 - -1.1 101,270 - +0.2
Or see this region's nucleotide sequence