Experiment: nitrogen source nitrite 0.5mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5638 and Pf1N1B4_5639 are separated by 141 nucleotides Pf1N1B4_5639 and Pf1N1B4_5640 are separated by 39 nucleotides Pf1N1B4_5640 and Pf1N1B4_5641 are separated by 130 nucleotides
Pf1N1B4_5638 - 2-dehydro-3-deoxygluconate kinase (EC 2.7.1.45), at 68,461 to 69,393
_5638
Pf1N1B4_5639 - D-serine deaminase (EC 4.3.1.18), at 69,535 to 70,752
_5639
Pf1N1B4_5640 - Transcriptional regulator, IclR family, at 70,792 to 71,571
_5640
Pf1N1B4_5641 - Bona fide RidA/YjgF/TdcF/RutC subgroup, at 71,702 to 72,091
_5641
Position (kb) 69
70
71 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 68.799 kb on - strand, within Pf1N1B4_5638 at 68.972 kb on + strand at 68.984 kb on + strand, within Pf1N1B4_5638 at 69.765 kb on - strand, within Pf1N1B4_5639 at 69.798 kb on - strand, within Pf1N1B4_5639 at 69.942 kb on - strand, within Pf1N1B4_5639 at 69.961 kb on + strand, within Pf1N1B4_5639 at 69.962 kb on - strand, within Pf1N1B4_5639 at 69.962 kb on - strand, within Pf1N1B4_5639 at 69.965 kb on + strand, within Pf1N1B4_5639 at 69.965 kb on + strand, within Pf1N1B4_5639 at 69.966 kb on - strand, within Pf1N1B4_5639 at 69.966 kb on - strand at 69.966 kb on - strand at 69.966 kb on - strand, within Pf1N1B4_5639 at 69.966 kb on - strand, within Pf1N1B4_5639 at 70.040 kb on + strand, within Pf1N1B4_5639 at 70.040 kb on + strand at 70.334 kb on + strand, within Pf1N1B4_5639 at 70.516 kb on - strand at 70.748 kb on + strand at 70.803 kb on + strand at 70.847 kb on + strand at 70.856 kb on + strand at 70.856 kb on + strand at 70.857 kb on - strand at 71.208 kb on + strand, within Pf1N1B4_5640 at 71.208 kb on + strand, within Pf1N1B4_5640 at 71.208 kb on + strand, within Pf1N1B4_5640 at 71.209 kb on - strand, within Pf1N1B4_5640 at 71.210 kb on + strand, within Pf1N1B4_5640 at 71.333 kb on + strand, within Pf1N1B4_5640 at 71.333 kb on + strand at 71.334 kb on - strand, within Pf1N1B4_5640 at 71.334 kb on - strand, within Pf1N1B4_5640 at 71.334 kb on - strand, within Pf1N1B4_5640 at 71.352 kb on - strand, within Pf1N1B4_5640 at 71.590 kb on + strand at 71.590 kb on + strand at 71.591 kb on - strand at 71.600 kb on - strand at 71.637 kb on - strand at 71.719 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source nitrite 0.5mM remove 68,799 - Pf1N1B4_5638 0.36 -0.5 68,972 + -0.9 68,984 + Pf1N1B4_5638 0.56 -2.8 69,765 - Pf1N1B4_5639 0.19 -0.3 69,798 - Pf1N1B4_5639 0.22 -1.1 69,942 - Pf1N1B4_5639 0.33 -1.5 69,961 + Pf1N1B4_5639 0.35 +1.0 69,962 - Pf1N1B4_5639 0.35 -1.9 69,962 - Pf1N1B4_5639 0.35 +0.6 69,965 + Pf1N1B4_5639 0.35 +0.2 69,965 + Pf1N1B4_5639 0.35 -0.9 69,966 - Pf1N1B4_5639 0.35 -0.1 69,966 - +0.1 69,966 - +2.6 69,966 - Pf1N1B4_5639 0.35 -0.9 69,966 - Pf1N1B4_5639 0.35 -1.2 70,040 + Pf1N1B4_5639 0.41 -1.8 70,040 + -1.7 70,334 + Pf1N1B4_5639 0.66 -2.4 70,516 - -0.7 70,748 + -0.4 70,803 + -2.7 70,847 + +0.9 70,856 + -1.0 70,856 + -2.3 70,857 - -0.6 71,208 + Pf1N1B4_5640 0.53 -0.2 71,208 + Pf1N1B4_5640 0.53 -1.9 71,208 + Pf1N1B4_5640 0.53 -2.5 71,209 - Pf1N1B4_5640 0.53 -0.0 71,210 + Pf1N1B4_5640 0.54 -0.0 71,333 + Pf1N1B4_5640 0.69 +0.9 71,333 + -0.7 71,334 - Pf1N1B4_5640 0.69 -0.3 71,334 - Pf1N1B4_5640 0.69 +0.5 71,334 - Pf1N1B4_5640 0.69 +0.6 71,352 - Pf1N1B4_5640 0.72 -0.7 71,590 + +2.6 71,590 + +0.0 71,591 - -1.0 71,600 - -2.2 71,637 - +0.6 71,719 - +0.9
Or see this region's nucleotide sequence