Experiment: nitrogen source nitrite 0.5mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5559 and Pf1N1B4_5560 are separated by 324 nucleotides Pf1N1B4_5560 and Pf1N1B4_5561 are separated by 281 nucleotides Pf1N1B4_5561 and Pf1N1B4_5562 are separated by 16 nucleotides
Pf1N1B4_5559 - hypothetical protein, at 329,864 to 329,986
_5559
Pf1N1B4_5560 - hypothetical protein, at 330,311 to 330,520
_5560
Pf1N1B4_5561 - hypothetical protein, at 330,802 to 331,050
_5561
Pf1N1B4_5562 - DNA/RNA non-specific endonuclease, at 331,067 to 331,942
_5562
Position (kb) 330
331 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 329.342 kb on - strand at 329.455 kb on + strand at 329.455 kb on + strand at 329.455 kb on + strand at 329.468 kb on - strand at 329.498 kb on + strand at 329.498 kb on + strand at 329.498 kb on + strand at 329.498 kb on + strand at 329.499 kb on - strand at 329.565 kb on - strand at 329.617 kb on + strand at 329.618 kb on - strand at 329.630 kb on + strand at 329.630 kb on + strand at 329.631 kb on - strand at 329.631 kb on - strand at 329.689 kb on + strand at 329.689 kb on + strand at 329.689 kb on + strand at 329.689 kb on + strand at 329.690 kb on - strand at 329.690 kb on - strand at 329.690 kb on - strand at 329.690 kb on - strand at 329.690 kb on - strand at 329.704 kb on + strand at 329.705 kb on - strand at 329.705 kb on - strand at 329.705 kb on - strand at 329.865 kb on + strand at 329.865 kb on + strand at 329.866 kb on - strand at 329.866 kb on - strand at 329.866 kb on - strand at 329.866 kb on - strand at 329.866 kb on - strand at 329.866 kb on - strand at 329.866 kb on - strand at 329.866 kb on - strand at 330.312 kb on + strand at 330.357 kb on - strand, within Pf1N1B4_5560 at 330.357 kb on - strand at 330.474 kb on + strand, within Pf1N1B4_5560 at 330.474 kb on + strand, within Pf1N1B4_5560 at 330.535 kb on - strand at 330.563 kb on + strand at 330.563 kb on + strand at 330.803 kb on + strand at 330.803 kb on + strand at 330.804 kb on - strand at 330.804 kb on - strand at 330.804 kb on - strand at 330.804 kb on - strand at 330.804 kb on - strand at 330.804 kb on - strand at 330.804 kb on - strand at 330.804 kb on - strand at 330.804 kb on - strand at 330.804 kb on - strand at 330.804 kb on - strand at 330.804 kb on - strand at 330.804 kb on - strand at 330.804 kb on - strand at 330.834 kb on + strand, within Pf1N1B4_5561 at 330.835 kb on - strand, within Pf1N1B4_5561 at 330.835 kb on - strand, within Pf1N1B4_5561 at 330.846 kb on - strand, within Pf1N1B4_5561 at 330.846 kb on - strand, within Pf1N1B4_5561 at 330.846 kb on - strand, within Pf1N1B4_5561 at 330.846 kb on - strand, within Pf1N1B4_5561 at 330.900 kb on - strand, within Pf1N1B4_5561 at 330.996 kb on - strand, within Pf1N1B4_5561 at 330.996 kb on - strand at 330.996 kb on - strand, within Pf1N1B4_5561 at 331.040 kb on + strand at 331.041 kb on - strand at 331.041 kb on - strand at 331.230 kb on + strand, within Pf1N1B4_5562 at 331.230 kb on + strand, within Pf1N1B4_5562 at 331.231 kb on - strand, within Pf1N1B4_5562 at 331.231 kb on - strand, within Pf1N1B4_5562 at 331.231 kb on - strand, within Pf1N1B4_5562 at 331.302 kb on + strand, within Pf1N1B4_5562 at 331.302 kb on + strand, within Pf1N1B4_5562 at 331.435 kb on + strand, within Pf1N1B4_5562 at 331.436 kb on - strand, within Pf1N1B4_5562 at 331.436 kb on - strand, within Pf1N1B4_5562
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source nitrite 0.5mM remove 329,342 - +0.2 329,455 + +1.1 329,455 + -1.7 329,455 + -1.1 329,468 - +0.6 329,498 + -1.0 329,498 + -0.9 329,498 + -0.8 329,498 + -1.6 329,499 - +0.2 329,565 - +0.2 329,617 + +0.7 329,618 - -1.1 329,630 + +0.8 329,630 + -0.7 329,631 - -1.0 329,631 - +1.0 329,689 + -0.9 329,689 + +0.2 329,689 + +0.2 329,689 + -0.1 329,690 - -0.8 329,690 - +2.0 329,690 - +2.2 329,690 - +0.6 329,690 - -0.2 329,704 + -2.1 329,705 - -0.0 329,705 - -0.0 329,705 - +0.8 329,865 + -1.2 329,865 + +0.5 329,866 - -1.3 329,866 - +1.2 329,866 - +0.0 329,866 - -2.2 329,866 - +0.3 329,866 - -0.5 329,866 - -0.7 329,866 - -0.2 330,312 + +1.7 330,357 - Pf1N1B4_5560 0.22 -1.2 330,357 - -1.6 330,474 + Pf1N1B4_5560 0.78 +0.0 330,474 + Pf1N1B4_5560 0.78 -1.1 330,535 - -1.1 330,563 + +1.3 330,563 + -1.9 330,803 + -0.1 330,803 + +1.4 330,804 - +3.3 330,804 - -0.3 330,804 - -1.0 330,804 - -0.4 330,804 - -1.9 330,804 - -0.8 330,804 - -0.8 330,804 - -0.2 330,804 - -0.1 330,804 - -1.9 330,804 - -1.5 330,804 - -1.2 330,804 - +0.4 330,804 - +1.0 330,834 + Pf1N1B4_5561 0.13 -0.9 330,835 - Pf1N1B4_5561 0.13 -2.1 330,835 - Pf1N1B4_5561 0.13 +0.0 330,846 - Pf1N1B4_5561 0.18 +1.0 330,846 - Pf1N1B4_5561 0.18 -0.6 330,846 - Pf1N1B4_5561 0.18 +0.1 330,846 - Pf1N1B4_5561 0.18 +0.7 330,900 - Pf1N1B4_5561 0.39 -1.5 330,996 - Pf1N1B4_5561 0.78 +0.5 330,996 - +1.1 330,996 - Pf1N1B4_5561 0.78 -0.0 331,040 + +2.0 331,041 - +0.2 331,041 - +0.6 331,230 + Pf1N1B4_5562 0.19 -0.7 331,230 + Pf1N1B4_5562 0.19 -0.5 331,231 - Pf1N1B4_5562 0.19 -0.1 331,231 - Pf1N1B4_5562 0.19 -3.0 331,231 - Pf1N1B4_5562 0.19 +0.1 331,302 + Pf1N1B4_5562 0.27 -0.6 331,302 + Pf1N1B4_5562 0.27 +0.3 331,435 + Pf1N1B4_5562 0.42 +1.4 331,436 - Pf1N1B4_5562 0.42 -2.6 331,436 - Pf1N1B4_5562 0.42 -1.3
Or see this region's nucleotide sequence