Experiment: nitrogen source nitrite 0.5mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5289 and Pf1N1B4_5290 are separated by 351 nucleotides Pf1N1B4_5290 and Pf1N1B4_5291 are separated by 290 nucleotides
Pf1N1B4_5289 - hypothetical protein, at 43,736 to 44,062
_5289
Pf1N1B4_5290 - hypothetical protein, at 44,414 to 44,644
_5290
Pf1N1B4_5291 - Putative reductase, at 44,935 to 46,248
_5291
Position (kb)
43
44
45 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 42.747 kb on + strand at 42.748 kb on - strand at 42.784 kb on + strand at 42.814 kb on + strand at 42.849 kb on + strand at 42.940 kb on + strand at 42.940 kb on + strand at 43.041 kb on - strand at 43.042 kb on + strand at 43.042 kb on + strand at 43.042 kb on + strand at 43.042 kb on + strand at 43.042 kb on + strand at 43.042 kb on + strand at 43.043 kb on - strand at 43.043 kb on - strand at 43.043 kb on - strand at 43.043 kb on - strand at 43.099 kb on - strand at 43.099 kb on - strand at 43.104 kb on - strand at 43.155 kb on - strand at 43.332 kb on - strand at 43.413 kb on + strand at 43.413 kb on + strand at 43.510 kb on + strand at 43.606 kb on + strand at 43.765 kb on + strand at 43.765 kb on + strand at 43.765 kb on + strand at 43.765 kb on + strand at 43.765 kb on + strand at 43.766 kb on - strand at 43.766 kb on - strand at 43.766 kb on - strand at 43.766 kb on - strand at 43.766 kb on - strand at 43.766 kb on - strand at 43.920 kb on - strand, within Pf1N1B4_5289 at 43.920 kb on - strand, within Pf1N1B4_5289 at 43.944 kb on + strand, within Pf1N1B4_5289 at 43.945 kb on - strand, within Pf1N1B4_5289 at 43.945 kb on - strand, within Pf1N1B4_5289 at 43.967 kb on - strand, within Pf1N1B4_5289 at 43.967 kb on - strand, within Pf1N1B4_5289 at 44.127 kb on - strand at 44.255 kb on + strand at 44.256 kb on - strand at 44.256 kb on - strand at 44.256 kb on - strand at 44.275 kb on + strand at 44.415 kb on + strand at 44.650 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source nitrite 0.5mM remove 42,747 + +0.2 42,748 - +1.0 42,784 + -2.4 42,814 + +0.4 42,849 + -0.3 42,940 + -0.7 42,940 + -0.6 43,041 - +1.9 43,042 + +1.1 43,042 + -2.1 43,042 + -1.0 43,042 + -2.1 43,042 + -0.5 43,042 + +1.4 43,043 - +0.3 43,043 - +0.4 43,043 - -0.7 43,043 - -0.6 43,099 - +2.3 43,099 - +0.2 43,104 - +0.7 43,155 - +2.2 43,332 - +1.3 43,413 + -0.7 43,413 + +0.3 43,510 + +0.1 43,606 + -0.4 43,765 + -0.8 43,765 + +0.6 43,765 + -0.6 43,765 + +0.0 43,765 + -1.2 43,766 - +0.4 43,766 - -0.4 43,766 - -1.8 43,766 - +0.0 43,766 - +1.1 43,766 - -1.1 43,920 - Pf1N1B4_5289 0.56 -2.0 43,920 - Pf1N1B4_5289 0.56 -2.2 43,944 + Pf1N1B4_5289 0.64 -1.7 43,945 - Pf1N1B4_5289 0.64 -1.3 43,945 - Pf1N1B4_5289 0.64 -0.2 43,967 - Pf1N1B4_5289 0.71 -1.0 43,967 - Pf1N1B4_5289 0.71 -0.7 44,127 - +1.6 44,255 + -1.6 44,256 - +0.5 44,256 - -0.7 44,256 - -0.4 44,275 + +1.0 44,415 + +0.9 44,650 - +2.0
Or see this region's nucleotide sequence