Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_5219

Experiment: nitrogen source nitrite 0.5mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_3295 and Pf1N1B4_5219 are separated by 333 nucleotidesPf1N1B4_5219 and Pf1N1B4_3296 are separated by 181 nucleotides Pf1N1B4_3295 - Filamentation induced by cAMP protein Fic, at 3,681,228 to 3,682,793 _3295 Pf1N1B4_5219 - tRNA-Gly-CCC, at 3,683,127 to 3,683,197 _5219 Pf1N1B4_3296 - (R)-3-hydroxydecanoyl-ACP:CoA transacylase PhaG (3-hydroxyacyl-CoA-acyl carrier protein transferase) (EC 2.4.1.-), at 3,683,379 to 3,684,263 _3296 Position (kb) 3683 3684Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 3682.257 kb on + strand, within Pf1N1B4_3295at 3682.257 kb on + strand, within Pf1N1B4_3295at 3682.257 kb on + strand, within Pf1N1B4_3295at 3682.257 kb on + strand, within Pf1N1B4_3295at 3682.257 kb on + strand, within Pf1N1B4_3295at 3682.278 kb on + strand, within Pf1N1B4_3295at 3682.405 kb on + strand, within Pf1N1B4_3295at 3682.405 kb on + strand, within Pf1N1B4_3295at 3682.406 kb on - strand, within Pf1N1B4_3295at 3682.406 kb on - strand, within Pf1N1B4_3295at 3682.422 kb on + strand, within Pf1N1B4_3295at 3682.432 kb on + strand, within Pf1N1B4_3295at 3682.433 kb on - strand, within Pf1N1B4_3295at 3682.510 kb on + strand, within Pf1N1B4_3295at 3682.561 kb on - strand, within Pf1N1B4_3295at 3682.561 kb on - strand, within Pf1N1B4_3295at 3682.606 kb on + strand, within Pf1N1B4_3295at 3682.607 kb on - strand, within Pf1N1B4_3295at 3682.707 kb on + strandat 3682.707 kb on + strandat 3682.707 kb on + strandat 3682.707 kb on + strandat 3682.867 kb on - strandat 3682.879 kb on - strandat 3682.972 kb on - strandat 3683.078 kb on - strandat 3683.176 kb on - strand, within Pf1N1B4_5219at 3683.185 kb on - strand, within Pf1N1B4_5219at 3683.186 kb on + strand, within Pf1N1B4_5219at 3683.186 kb on + strand, within Pf1N1B4_5219at 3683.187 kb on - strand, within Pf1N1B4_5219at 3683.187 kb on - strand, within Pf1N1B4_5219at 3683.187 kb on - strand, within Pf1N1B4_5219at 3683.187 kb on - strand, within Pf1N1B4_5219at 3683.187 kb on - strandat 3683.187 kb on - strandat 3683.265 kb on + strandat 3683.265 kb on + strandat 3683.326 kb on + strandat 3683.411 kb on - strandat 3683.411 kb on - strandat 3683.411 kb on - strandat 3683.590 kb on + strandat 3683.590 kb on + strand, within Pf1N1B4_3296at 3683.590 kb on + strand, within Pf1N1B4_3296at 3683.591 kb on - strand, within Pf1N1B4_3296at 3683.591 kb on - strand, within Pf1N1B4_3296at 3683.653 kb on + strand, within Pf1N1B4_3296at 3683.740 kb on + strand, within Pf1N1B4_3296at 3683.740 kb on + strand, within Pf1N1B4_3296at 3683.831 kb on - strandat 3683.851 kb on + strand, within Pf1N1B4_3296at 3684.088 kb on + strand, within Pf1N1B4_3296at 3684.173 kb on + strand, within Pf1N1B4_3296at 3684.173 kb on + strand, within Pf1N1B4_3296at 3684.173 kb on + strand, within Pf1N1B4_3296

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Per-strain Table

Position Strand Gene LocusTag Fraction nitrogen source nitrite 0.5mM
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3,682,257 + Pf1N1B4_3295 0.66 +0.3
3,682,257 + Pf1N1B4_3295 0.66 +0.6
3,682,257 + Pf1N1B4_3295 0.66 -0.1
3,682,257 + Pf1N1B4_3295 0.66 -0.2
3,682,257 + Pf1N1B4_3295 0.66 +0.1
3,682,278 + Pf1N1B4_3295 0.67 -0.4
3,682,405 + Pf1N1B4_3295 0.75 -0.1
3,682,405 + Pf1N1B4_3295 0.75 +2.9
3,682,406 - Pf1N1B4_3295 0.75 -1.0
3,682,406 - Pf1N1B4_3295 0.75 -1.0
3,682,422 + Pf1N1B4_3295 0.76 -0.1
3,682,432 + Pf1N1B4_3295 0.77 +0.5
3,682,433 - Pf1N1B4_3295 0.77 -0.2
3,682,510 + Pf1N1B4_3295 0.82 -1.7
3,682,561 - Pf1N1B4_3295 0.85 +0.2
3,682,561 - Pf1N1B4_3295 0.85 +1.6
3,682,606 + Pf1N1B4_3295 0.88 -0.5
3,682,607 - Pf1N1B4_3295 0.88 -0.7
3,682,707 + +2.0
3,682,707 + -1.7
3,682,707 + +0.2
3,682,707 + +0.5
3,682,867 - -0.1
3,682,879 - -1.3
3,682,972 - -0.5
3,683,078 - +0.6
3,683,176 - Pf1N1B4_5219 0.69 -1.7
3,683,185 - Pf1N1B4_5219 0.82 -1.1
3,683,186 + Pf1N1B4_5219 0.83 -1.0
3,683,186 + Pf1N1B4_5219 0.83 -2.5
3,683,187 - Pf1N1B4_5219 0.85 +0.3
3,683,187 - Pf1N1B4_5219 0.85 +0.1
3,683,187 - Pf1N1B4_5219 0.85 -1.9
3,683,187 - Pf1N1B4_5219 0.85 +0.5
3,683,187 - -0.5
3,683,187 - +1.0
3,683,265 + -3.1
3,683,265 + +0.1
3,683,326 + -0.3
3,683,411 - +0.6
3,683,411 - -0.2
3,683,411 - -1.1
3,683,590 + -2.0
3,683,590 + Pf1N1B4_3296 0.24 -0.5
3,683,590 + Pf1N1B4_3296 0.24 -1.5
3,683,591 - Pf1N1B4_3296 0.24 -2.5
3,683,591 - Pf1N1B4_3296 0.24 -2.5
3,683,653 + Pf1N1B4_3296 0.31 -0.6
3,683,740 + Pf1N1B4_3296 0.41 -1.6
3,683,740 + Pf1N1B4_3296 0.41 -1.6
3,683,831 - +0.4
3,683,851 + Pf1N1B4_3296 0.53 -1.1
3,684,088 + Pf1N1B4_3296 0.80 -2.0
3,684,173 + Pf1N1B4_3296 0.90 -0.4
3,684,173 + Pf1N1B4_3296 0.90 -2.0
3,684,173 + Pf1N1B4_3296 0.90 -0.6

Or see this region's nucleotide sequence