Experiment: nitrogen source nitrite 0.5mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4684 and Pf1N1B4_4685 overlap by 4 nucleotides Pf1N1B4_4685 and Pf1N1B4_4686 overlap by 4 nucleotides
Pf1N1B4_4684 - Malto-oligosyltrehalose synthase (EC 5.4.99.15), at 5,171,667 to 5,174,465
_4684
Pf1N1B4_4685 - 4-alpha-glucanotransferase (amylomaltase) (EC 2.4.1.25), at 5,174,462 to 5,176,540
_4685
Pf1N1B4_4686 - Malto-oligosyltrehalose trehalohydrolase (EC 3.2.1.141), at 5,176,537 to 5,178,291
_4686
Position (kb) 5174
5175
5176
5177 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 5173.720 kb on + strand, within Pf1N1B4_4684 at 5173.720 kb on + strand at 5173.720 kb on + strand, within Pf1N1B4_4684 at 5173.720 kb on + strand, within Pf1N1B4_4684 at 5173.721 kb on - strand, within Pf1N1B4_4684 at 5173.721 kb on - strand, within Pf1N1B4_4684 at 5173.835 kb on + strand, within Pf1N1B4_4684 at 5174.342 kb on - strand at 5174.371 kb on + strand at 5174.947 kb on + strand, within Pf1N1B4_4685 at 5175.111 kb on + strand, within Pf1N1B4_4685 at 5175.117 kb on + strand, within Pf1N1B4_4685 at 5175.499 kb on - strand at 5175.723 kb on + strand, within Pf1N1B4_4685 at 5175.723 kb on + strand, within Pf1N1B4_4685 at 5175.724 kb on - strand, within Pf1N1B4_4685 at 5176.062 kb on + strand, within Pf1N1B4_4685 at 5176.062 kb on + strand, within Pf1N1B4_4685 at 5176.063 kb on - strand, within Pf1N1B4_4685 at 5176.126 kb on - strand, within Pf1N1B4_4685 at 5176.186 kb on - strand, within Pf1N1B4_4685 at 5176.290 kb on + strand, within Pf1N1B4_4685 at 5176.291 kb on - strand, within Pf1N1B4_4685 at 5176.517 kb on + strand at 5176.517 kb on + strand at 5176.823 kb on + strand, within Pf1N1B4_4686 at 5176.823 kb on + strand, within Pf1N1B4_4686 at 5176.823 kb on + strand, within Pf1N1B4_4686 at 5176.823 kb on + strand, within Pf1N1B4_4686 at 5176.824 kb on - strand, within Pf1N1B4_4686 at 5176.824 kb on - strand, within Pf1N1B4_4686 at 5176.824 kb on - strand, within Pf1N1B4_4686 at 5176.824 kb on - strand, within Pf1N1B4_4686 at 5176.824 kb on - strand, within Pf1N1B4_4686 at 5176.824 kb on - strand, within Pf1N1B4_4686 at 5176.824 kb on - strand at 5177.351 kb on + strand, within Pf1N1B4_4686 at 5177.351 kb on + strand, within Pf1N1B4_4686
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source nitrite 0.5mM remove 5,173,720 + Pf1N1B4_4684 0.73 +0.9 5,173,720 + +0.4 5,173,720 + Pf1N1B4_4684 0.73 -0.3 5,173,720 + Pf1N1B4_4684 0.73 -0.6 5,173,721 - Pf1N1B4_4684 0.73 +0.4 5,173,721 - Pf1N1B4_4684 0.73 +1.0 5,173,835 + Pf1N1B4_4684 0.77 -0.3 5,174,342 - -0.1 5,174,371 + +0.5 5,174,947 + Pf1N1B4_4685 0.23 -0.2 5,175,111 + Pf1N1B4_4685 0.31 -3.3 5,175,117 + Pf1N1B4_4685 0.32 -1.9 5,175,499 - -2.0 5,175,723 + Pf1N1B4_4685 0.61 +0.0 5,175,723 + Pf1N1B4_4685 0.61 -0.6 5,175,724 - Pf1N1B4_4685 0.61 -1.1 5,176,062 + Pf1N1B4_4685 0.77 -0.3 5,176,062 + Pf1N1B4_4685 0.77 -0.9 5,176,063 - Pf1N1B4_4685 0.77 -0.3 5,176,126 - Pf1N1B4_4685 0.80 -0.3 5,176,186 - Pf1N1B4_4685 0.83 -1.1 5,176,290 + Pf1N1B4_4685 0.88 -0.6 5,176,291 - Pf1N1B4_4685 0.88 -0.9 5,176,517 + +0.3 5,176,517 + -2.6 5,176,823 + Pf1N1B4_4686 0.16 -1.9 5,176,823 + Pf1N1B4_4686 0.16 -1.3 5,176,823 + Pf1N1B4_4686 0.16 -0.0 5,176,823 + Pf1N1B4_4686 0.16 -0.6 5,176,824 - Pf1N1B4_4686 0.16 -1.0 5,176,824 - Pf1N1B4_4686 0.16 +2.1 5,176,824 - Pf1N1B4_4686 0.16 -0.7 5,176,824 - Pf1N1B4_4686 0.16 -0.3 5,176,824 - Pf1N1B4_4686 0.16 +0.6 5,176,824 - Pf1N1B4_4686 0.16 -0.1 5,176,824 - +1.2 5,177,351 + Pf1N1B4_4686 0.46 +0.7 5,177,351 + Pf1N1B4_4686 0.46 +1.2
Or see this region's nucleotide sequence