Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_463

Experiment: nitrogen source nitrite 0.5mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_461 and Pf1N1B4_462 are separated by 231 nucleotidesPf1N1B4_462 and Pf1N1B4_463 overlap by 4 nucleotidesPf1N1B4_463 and Pf1N1B4_464 overlap by 1 nucleotides Pf1N1B4_461 - Ribonuclease E (EC 3.1.26.12), at 550,465 to 553,638 _461 Pf1N1B4_462 - CTP:molybdopterin cytidylyltransferase, at 553,870 to 554,463 _462 Pf1N1B4_463 - Xanthine and CO dehydrogenases maturation factor, XdhC/CoxF family, at 554,460 to 555,443 _463 Pf1N1B4_464 - Isoquinoline 1-oxidoreductase beta subunit (EC 1.3.99.16), at 555,443 to 557,764 _464 Position (kb) 554 555 556Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 553.690 kb on - strandat 553.690 kb on - strandat 554.054 kb on - strand, within Pf1N1B4_462at 554.118 kb on + strand, within Pf1N1B4_462at 554.235 kb on - strand, within Pf1N1B4_462at 554.463 kb on + strandat 554.464 kb on - strandat 554.558 kb on + strandat 554.558 kb on + strandat 554.558 kb on + strandat 554.558 kb on + strandat 554.559 kb on - strand, within Pf1N1B4_463at 554.622 kb on + strand, within Pf1N1B4_463at 554.623 kb on - strand, within Pf1N1B4_463at 554.623 kb on - strandat 554.623 kb on - strand, within Pf1N1B4_463at 554.623 kb on - strand, within Pf1N1B4_463at 554.684 kb on - strand, within Pf1N1B4_463at 554.684 kb on - strand, within Pf1N1B4_463at 554.684 kb on - strand, within Pf1N1B4_463at 554.842 kb on + strand, within Pf1N1B4_463at 554.842 kb on + strand, within Pf1N1B4_463at 554.842 kb on + strand, within Pf1N1B4_463at 554.843 kb on - strand, within Pf1N1B4_463at 554.911 kb on + strand, within Pf1N1B4_463at 554.962 kb on + strand, within Pf1N1B4_463at 554.962 kb on + strand, within Pf1N1B4_463at 554.962 kb on + strand, within Pf1N1B4_463at 554.962 kb on + strand, within Pf1N1B4_463at 554.963 kb on - strand, within Pf1N1B4_463at 554.963 kb on - strand, within Pf1N1B4_463at 554.963 kb on - strand, within Pf1N1B4_463at 554.963 kb on - strand, within Pf1N1B4_463at 554.963 kb on - strand, within Pf1N1B4_463at 555.194 kb on - strand, within Pf1N1B4_463at 555.197 kb on + strand, within Pf1N1B4_463at 555.197 kb on + strand, within Pf1N1B4_463at 555.198 kb on - strand, within Pf1N1B4_463at 555.201 kb on + strand, within Pf1N1B4_463at 555.201 kb on + strand, within Pf1N1B4_463at 555.202 kb on - strand, within Pf1N1B4_463at 555.202 kb on - strandat 555.202 kb on - strand, within Pf1N1B4_463at 555.370 kb on + strandat 555.370 kb on + strandat 555.371 kb on - strandat 555.371 kb on - strandat 555.478 kb on - strandat 555.478 kb on - strandat 555.654 kb on + strandat 555.654 kb on + strandat 555.654 kb on + strandat 555.654 kb on + strandat 555.654 kb on + strandat 555.655 kb on - strandat 555.655 kb on - strandat 555.655 kb on - strandat 555.655 kb on - strandat 555.655 kb on - strandat 555.655 kb on - strandat 555.655 kb on - strandat 555.864 kb on + strand, within Pf1N1B4_464at 555.864 kb on + strand, within Pf1N1B4_464at 555.864 kb on + strand, within Pf1N1B4_464at 555.864 kb on + strand, within Pf1N1B4_464at 555.864 kb on + strand, within Pf1N1B4_464at 555.864 kb on + strand, within Pf1N1B4_464at 555.864 kb on + strand, within Pf1N1B4_464at 555.865 kb on - strand, within Pf1N1B4_464at 555.865 kb on - strand, within Pf1N1B4_464at 555.865 kb on - strand, within Pf1N1B4_464at 555.865 kb on - strand, within Pf1N1B4_464at 555.865 kb on - strand, within Pf1N1B4_464at 555.865 kb on - strand, within Pf1N1B4_464at 555.865 kb on - strand, within Pf1N1B4_464at 555.865 kb on - strand, within Pf1N1B4_464at 555.865 kb on - strand, within Pf1N1B4_464at 555.865 kb on - strand, within Pf1N1B4_464at 555.865 kb on - strand, within Pf1N1B4_464at 555.877 kb on + strand, within Pf1N1B4_464at 555.877 kb on + strand, within Pf1N1B4_464at 555.878 kb on - strand, within Pf1N1B4_464at 555.878 kb on - strand, within Pf1N1B4_464at 555.878 kb on - strand, within Pf1N1B4_464at 555.878 kb on - strand, within Pf1N1B4_464at 555.878 kb on - strandat 555.878 kb on - strand, within Pf1N1B4_464at 555.878 kb on - strand, within Pf1N1B4_464at 555.878 kb on - strand, within Pf1N1B4_464at 555.878 kb on - strand, within Pf1N1B4_464at 555.878 kb on - strand, within Pf1N1B4_464at 555.880 kb on - strand, within Pf1N1B4_464at 555.957 kb on - strand, within Pf1N1B4_464at 556.008 kb on + strandat 556.008 kb on + strand, within Pf1N1B4_464at 556.075 kb on - strand, within Pf1N1B4_464at 556.075 kb on - strand, within Pf1N1B4_464at 556.228 kb on - strand, within Pf1N1B4_464at 556.241 kb on - strandat 556.303 kb on + strand, within Pf1N1B4_464at 556.372 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction nitrogen source nitrite 0.5mM
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553,690 - +0.1
553,690 - +2.8
554,054 - Pf1N1B4_462 0.31 -1.0
554,118 + Pf1N1B4_462 0.42 +1.0
554,235 - Pf1N1B4_462 0.61 +0.6
554,463 + +0.1
554,464 - -2.1
554,558 + +1.1
554,558 + +0.8
554,558 + +0.6
554,558 + +0.0
554,559 - Pf1N1B4_463 0.10 +1.1
554,622 + Pf1N1B4_463 0.16 +1.1
554,623 - Pf1N1B4_463 0.17 -1.8
554,623 - -2.3
554,623 - Pf1N1B4_463 0.17 -0.7
554,623 - Pf1N1B4_463 0.17 +0.1
554,684 - Pf1N1B4_463 0.23 -1.3
554,684 - Pf1N1B4_463 0.23 -0.2
554,684 - Pf1N1B4_463 0.23 +0.8
554,842 + Pf1N1B4_463 0.39 -2.2
554,842 + Pf1N1B4_463 0.39 +1.4
554,842 + Pf1N1B4_463 0.39 -2.0
554,843 - Pf1N1B4_463 0.39 -0.8
554,911 + Pf1N1B4_463 0.46 +0.4
554,962 + Pf1N1B4_463 0.51 +0.4
554,962 + Pf1N1B4_463 0.51 +0.5
554,962 + Pf1N1B4_463 0.51 +0.1
554,962 + Pf1N1B4_463 0.51 +0.3
554,963 - Pf1N1B4_463 0.51 +0.3
554,963 - Pf1N1B4_463 0.51 -0.4
554,963 - Pf1N1B4_463 0.51 +0.3
554,963 - Pf1N1B4_463 0.51 -2.7
554,963 - Pf1N1B4_463 0.51 -0.0
555,194 - Pf1N1B4_463 0.75 -0.7
555,197 + Pf1N1B4_463 0.75 -0.2
555,197 + Pf1N1B4_463 0.75 -0.2
555,198 - Pf1N1B4_463 0.75 +0.7
555,201 + Pf1N1B4_463 0.75 +1.2
555,201 + Pf1N1B4_463 0.75 +0.3
555,202 - Pf1N1B4_463 0.75 -2.1
555,202 - +0.2
555,202 - Pf1N1B4_463 0.75 +0.4
555,370 + -0.7
555,370 + -0.6
555,371 - +0.0
555,371 - -0.2
555,478 - +1.3
555,478 - +1.8
555,654 + +2.8
555,654 + +2.3
555,654 + -0.7
555,654 + -1.0
555,654 + -0.3
555,655 - +0.3
555,655 - +0.2
555,655 - -2.1
555,655 - +1.0
555,655 - -1.4
555,655 - -1.2
555,655 - -0.2
555,864 + Pf1N1B4_464 0.18 -0.9
555,864 + Pf1N1B4_464 0.18 +1.6
555,864 + Pf1N1B4_464 0.18 -0.4
555,864 + Pf1N1B4_464 0.18 +0.5
555,864 + Pf1N1B4_464 0.18 +1.2
555,864 + Pf1N1B4_464 0.18 +0.7
555,864 + Pf1N1B4_464 0.18 -0.3
555,865 - Pf1N1B4_464 0.18 -0.7
555,865 - Pf1N1B4_464 0.18 +0.1
555,865 - Pf1N1B4_464 0.18 +1.1
555,865 - Pf1N1B4_464 0.18 -0.5
555,865 - Pf1N1B4_464 0.18 -0.8
555,865 - Pf1N1B4_464 0.18 +0.6
555,865 - Pf1N1B4_464 0.18 -0.2
555,865 - Pf1N1B4_464 0.18 +0.1
555,865 - Pf1N1B4_464 0.18 -0.9
555,865 - Pf1N1B4_464 0.18 -0.5
555,865 - Pf1N1B4_464 0.18 -0.4
555,877 + Pf1N1B4_464 0.19 -2.6
555,877 + Pf1N1B4_464 0.19 -1.1
555,878 - Pf1N1B4_464 0.19 -0.3
555,878 - Pf1N1B4_464 0.19 -0.0
555,878 - Pf1N1B4_464 0.19 -1.2
555,878 - Pf1N1B4_464 0.19 +1.5
555,878 - +0.3
555,878 - Pf1N1B4_464 0.19 +0.7
555,878 - Pf1N1B4_464 0.19 -2.4
555,878 - Pf1N1B4_464 0.19 +1.8
555,878 - Pf1N1B4_464 0.19 +0.1
555,878 - Pf1N1B4_464 0.19 +0.4
555,880 - Pf1N1B4_464 0.19 -2.5
555,957 - Pf1N1B4_464 0.22 +0.0
556,008 + +0.3
556,008 + Pf1N1B4_464 0.24 -2.9
556,075 - Pf1N1B4_464 0.27 -0.5
556,075 - Pf1N1B4_464 0.27 +0.2
556,228 - Pf1N1B4_464 0.34 +0.2
556,241 - +1.0
556,303 + Pf1N1B4_464 0.37 -0.7
556,372 - +1.5

Or see this region's nucleotide sequence