Experiment: nitrogen source nitrite 0.5mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4285 and Pf1N1B4_4286 are separated by 54 nucleotides Pf1N1B4_4286 and Pf1N1B4_4287 are separated by 57 nucleotides
Pf1N1B4_4285 - Inositol transport system sugar-binding protein, at 4,750,312 to 4,751,238
_4285
Pf1N1B4_4286 - Inositol transport system ATP-binding protein, at 4,751,293 to 4,752,873
_4286
Pf1N1B4_4287 - Inositol transport system permease protein, at 4,752,931 to 4,753,953
_4287
Position (kb)
4751
4752
4753 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 4750.383 kb on + strand at 4750.432 kb on + strand, within Pf1N1B4_4285 at 4750.535 kb on + strand, within Pf1N1B4_4285 at 4750.645 kb on + strand at 4750.645 kb on + strand, within Pf1N1B4_4285 at 4750.645 kb on + strand, within Pf1N1B4_4285 at 4750.739 kb on - strand, within Pf1N1B4_4285 at 4750.849 kb on + strand, within Pf1N1B4_4285 at 4751.236 kb on + strand at 4751.237 kb on - strand at 4751.261 kb on + strand at 4751.261 kb on + strand at 4751.262 kb on - strand at 4751.262 kb on - strand at 4751.262 kb on - strand at 4751.299 kb on + strand at 4751.383 kb on + strand at 4751.536 kb on + strand, within Pf1N1B4_4286 at 4751.849 kb on - strand, within Pf1N1B4_4286 at 4751.962 kb on + strand, within Pf1N1B4_4286 at 4751.962 kb on + strand, within Pf1N1B4_4286 at 4751.962 kb on + strand, within Pf1N1B4_4286 at 4751.962 kb on + strand, within Pf1N1B4_4286 at 4751.962 kb on + strand, within Pf1N1B4_4286 at 4751.963 kb on - strand, within Pf1N1B4_4286 at 4751.963 kb on - strand, within Pf1N1B4_4286 at 4751.963 kb on - strand, within Pf1N1B4_4286 at 4752.031 kb on + strand, within Pf1N1B4_4286 at 4752.031 kb on + strand, within Pf1N1B4_4286 at 4752.031 kb on + strand, within Pf1N1B4_4286 at 4752.032 kb on - strand, within Pf1N1B4_4286 at 4752.032 kb on - strand at 4752.454 kb on + strand at 4752.497 kb on + strand, within Pf1N1B4_4286 at 4752.498 kb on - strand, within Pf1N1B4_4286 at 4752.680 kb on - strand, within Pf1N1B4_4286 at 4752.687 kb on + strand, within Pf1N1B4_4286 at 4752.687 kb on + strand, within Pf1N1B4_4286 at 4752.687 kb on + strand, within Pf1N1B4_4286 at 4752.687 kb on + strand, within Pf1N1B4_4286 at 4752.687 kb on + strand, within Pf1N1B4_4286 at 4752.694 kb on + strand, within Pf1N1B4_4286 at 4752.694 kb on + strand, within Pf1N1B4_4286 at 4752.750 kb on - strand at 4752.914 kb on + strand at 4752.914 kb on + strand at 4752.914 kb on + strand at 4752.915 kb on - strand at 4753.005 kb on + strand at 4753.005 kb on + strand at 4753.005 kb on + strand at 4753.426 kb on + strand, within Pf1N1B4_4287 at 4753.427 kb on - strand, within Pf1N1B4_4287 at 4753.552 kb on + strand, within Pf1N1B4_4287 at 4753.553 kb on - strand, within Pf1N1B4_4287 at 4753.561 kb on + strand, within Pf1N1B4_4287 at 4753.561 kb on + strand, within Pf1N1B4_4287 at 4753.561 kb on + strand, within Pf1N1B4_4287 at 4753.562 kb on - strand, within Pf1N1B4_4287 at 4753.562 kb on - strand, within Pf1N1B4_4287 at 4753.562 kb on - strand, within Pf1N1B4_4287 at 4753.562 kb on - strand, within Pf1N1B4_4287 at 4753.562 kb on - strand, within Pf1N1B4_4287 at 4753.568 kb on - strand, within Pf1N1B4_4287 at 4753.639 kb on + strand, within Pf1N1B4_4287 at 4753.726 kb on + strand, within Pf1N1B4_4287 at 4753.727 kb on - strand, within Pf1N1B4_4287 at 4753.727 kb on - strand, within Pf1N1B4_4287 at 4753.727 kb on - strand, within Pf1N1B4_4287 at 4753.846 kb on - strand, within Pf1N1B4_4287 at 4753.867 kb on + strand at 4753.867 kb on + strand at 4753.868 kb on - strand at 4753.868 kb on - strand at 4753.868 kb on - strand at 4753.868 kb on - strand at 4753.869 kb on + strand at 4753.869 kb on + strand at 4753.869 kb on + strand at 4753.870 kb on - strand at 4753.870 kb on - strand at 4753.870 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source nitrite 0.5mM remove 4,750,383 + +2.4 4,750,432 + Pf1N1B4_4285 0.13 -1.0 4,750,535 + Pf1N1B4_4285 0.24 -1.5 4,750,645 + -0.7 4,750,645 + Pf1N1B4_4285 0.36 -3.5 4,750,645 + Pf1N1B4_4285 0.36 +0.6 4,750,739 - Pf1N1B4_4285 0.46 -2.5 4,750,849 + Pf1N1B4_4285 0.58 -0.3 4,751,236 + -0.1 4,751,237 - -0.2 4,751,261 + +0.5 4,751,261 + +0.9 4,751,262 - +0.2 4,751,262 - -1.9 4,751,262 - +1.1 4,751,299 + -0.9 4,751,383 + -0.4 4,751,536 + Pf1N1B4_4286 0.15 -0.4 4,751,849 - Pf1N1B4_4286 0.35 -2.8 4,751,962 + Pf1N1B4_4286 0.42 -0.9 4,751,962 + Pf1N1B4_4286 0.42 +0.3 4,751,962 + Pf1N1B4_4286 0.42 -0.6 4,751,962 + Pf1N1B4_4286 0.42 +0.4 4,751,962 + Pf1N1B4_4286 0.42 -1.2 4,751,963 - Pf1N1B4_4286 0.42 +0.3 4,751,963 - Pf1N1B4_4286 0.42 -0.4 4,751,963 - Pf1N1B4_4286 0.42 -0.1 4,752,031 + Pf1N1B4_4286 0.47 +1.8 4,752,031 + Pf1N1B4_4286 0.47 -0.2 4,752,031 + Pf1N1B4_4286 0.47 +0.3 4,752,032 - Pf1N1B4_4286 0.47 -1.4 4,752,032 - -1.4 4,752,454 + +0.2 4,752,497 + Pf1N1B4_4286 0.76 -0.8 4,752,498 - Pf1N1B4_4286 0.76 -2.6 4,752,680 - Pf1N1B4_4286 0.88 -0.3 4,752,687 + Pf1N1B4_4286 0.88 -1.0 4,752,687 + Pf1N1B4_4286 0.88 -2.1 4,752,687 + Pf1N1B4_4286 0.88 +0.9 4,752,687 + Pf1N1B4_4286 0.88 -0.8 4,752,687 + Pf1N1B4_4286 0.88 -1.9 4,752,694 + Pf1N1B4_4286 0.89 -0.7 4,752,694 + Pf1N1B4_4286 0.89 +0.7 4,752,750 - +0.2 4,752,914 + -0.2 4,752,914 + +0.2 4,752,914 + +0.2 4,752,915 - -0.1 4,753,005 + -2.1 4,753,005 + +0.9 4,753,005 + +3.0 4,753,426 + Pf1N1B4_4287 0.48 +0.4 4,753,427 - Pf1N1B4_4287 0.48 +0.7 4,753,552 + Pf1N1B4_4287 0.61 +0.5 4,753,553 - Pf1N1B4_4287 0.61 +0.6 4,753,561 + Pf1N1B4_4287 0.62 +0.0 4,753,561 + Pf1N1B4_4287 0.62 -0.2 4,753,561 + Pf1N1B4_4287 0.62 -0.2 4,753,562 - Pf1N1B4_4287 0.62 +0.2 4,753,562 - Pf1N1B4_4287 0.62 +0.4 4,753,562 - Pf1N1B4_4287 0.62 +1.1 4,753,562 - Pf1N1B4_4287 0.62 -1.0 4,753,562 - Pf1N1B4_4287 0.62 +0.6 4,753,568 - Pf1N1B4_4287 0.62 -0.1 4,753,639 + Pf1N1B4_4287 0.69 -1.6 4,753,726 + Pf1N1B4_4287 0.78 -0.0 4,753,727 - Pf1N1B4_4287 0.78 -0.8 4,753,727 - Pf1N1B4_4287 0.78 -0.9 4,753,727 - Pf1N1B4_4287 0.78 -3.1 4,753,846 - Pf1N1B4_4287 0.89 -1.2 4,753,867 + -0.8 4,753,867 + +0.1 4,753,868 - -1.2 4,753,868 - -0.4 4,753,868 - -0.7 4,753,868 - -2.2 4,753,869 + -0.1 4,753,869 + +0.9 4,753,869 + -0.3 4,753,870 - +0.1 4,753,870 - +0.1 4,753,870 - -0.9
Or see this region's nucleotide sequence