Experiment: nitrogen source nitrite 0.5mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3850 and Pf1N1B4_3851 are separated by 41 nucleotides Pf1N1B4_3851 and Pf1N1B4_3852 are separated by 127 nucleotides
Pf1N1B4_3850 - Guanine deaminase (EC 3.5.4.3), at 4,271,329 to 4,272,633
_3850
Pf1N1B4_3851 - XdhC protein (assists in molybdopterin insertion into xanthine dehydrogenase), at 4,272,675 to 4,273,529
_3851
Pf1N1B4_3852 - Xanthine dehydrogenase, molybdenum binding subunit (EC 1.17.1.4), at 4,273,657 to 4,276,053
_3852
Position (kb)
4272
4273
4274 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4271.853 kb on - strand, within Pf1N1B4_3850 at 4271.861 kb on + strand, within Pf1N1B4_3850 at 4271.862 kb on - strand, within Pf1N1B4_3850 at 4271.862 kb on - strand at 4271.948 kb on + strand, within Pf1N1B4_3850 at 4271.949 kb on - strand, within Pf1N1B4_3850 at 4271.949 kb on - strand, within Pf1N1B4_3850 at 4271.949 kb on - strand, within Pf1N1B4_3850 at 4271.949 kb on - strand, within Pf1N1B4_3850 at 4271.949 kb on - strand, within Pf1N1B4_3850 at 4272.089 kb on + strand, within Pf1N1B4_3850 at 4272.090 kb on - strand, within Pf1N1B4_3850 at 4272.090 kb on - strand, within Pf1N1B4_3850 at 4272.090 kb on - strand, within Pf1N1B4_3850 at 4272.117 kb on - strand at 4272.117 kb on - strand at 4272.332 kb on + strand, within Pf1N1B4_3850 at 4272.332 kb on + strand, within Pf1N1B4_3850 at 4272.332 kb on + strand, within Pf1N1B4_3850 at 4272.332 kb on + strand, within Pf1N1B4_3850 at 4272.332 kb on + strand, within Pf1N1B4_3850 at 4272.333 kb on - strand, within Pf1N1B4_3850 at 4272.333 kb on - strand, within Pf1N1B4_3850 at 4272.333 kb on - strand, within Pf1N1B4_3850 at 4272.333 kb on - strand, within Pf1N1B4_3850 at 4272.333 kb on - strand, within Pf1N1B4_3850 at 4272.333 kb on - strand at 4272.333 kb on - strand, within Pf1N1B4_3850 at 4272.352 kb on + strand, within Pf1N1B4_3850 at 4272.352 kb on + strand, within Pf1N1B4_3850 at 4272.352 kb on + strand, within Pf1N1B4_3850 at 4272.352 kb on + strand, within Pf1N1B4_3850 at 4272.366 kb on - strand, within Pf1N1B4_3850 at 4272.551 kb on + strand at 4272.647 kb on + strand at 4272.647 kb on + strand at 4272.648 kb on - strand at 4272.766 kb on + strand, within Pf1N1B4_3851 at 4272.766 kb on + strand, within Pf1N1B4_3851 at 4272.766 kb on + strand at 4272.766 kb on + strand, within Pf1N1B4_3851 at 4272.766 kb on + strand, within Pf1N1B4_3851 at 4272.766 kb on + strand, within Pf1N1B4_3851 at 4272.766 kb on + strand, within Pf1N1B4_3851 at 4272.766 kb on + strand, within Pf1N1B4_3851 at 4272.767 kb on - strand, within Pf1N1B4_3851 at 4272.767 kb on - strand, within Pf1N1B4_3851 at 4273.021 kb on + strand, within Pf1N1B4_3851 at 4273.021 kb on + strand, within Pf1N1B4_3851 at 4273.022 kb on - strand, within Pf1N1B4_3851 at 4273.354 kb on + strand, within Pf1N1B4_3851 at 4273.354 kb on + strand, within Pf1N1B4_3851 at 4273.354 kb on + strand, within Pf1N1B4_3851 at 4273.355 kb on - strand, within Pf1N1B4_3851 at 4273.355 kb on - strand, within Pf1N1B4_3851 at 4273.516 kb on + strand at 4273.516 kb on + strand at 4273.516 kb on + strand at 4273.516 kb on + strand at 4273.516 kb on + strand at 4273.516 kb on + strand at 4273.517 kb on - strand at 4273.517 kb on - strand at 4273.517 kb on - strand at 4273.517 kb on - strand at 4273.517 kb on - strand at 4273.517 kb on - strand at 4273.517 kb on - strand at 4273.517 kb on - strand at 4273.517 kb on - strand at 4273.952 kb on + strand, within Pf1N1B4_3852 at 4273.953 kb on - strand, within Pf1N1B4_3852 at 4273.953 kb on - strand, within Pf1N1B4_3852 at 4274.120 kb on + strand, within Pf1N1B4_3852 at 4274.175 kb on - strand, within Pf1N1B4_3852 at 4274.177 kb on + strand, within Pf1N1B4_3852 at 4274.178 kb on - strand at 4274.178 kb on - strand at 4274.178 kb on - strand at 4274.178 kb on - strand, within Pf1N1B4_3852 at 4274.178 kb on - strand, within Pf1N1B4_3852 at 4274.180 kb on + strand, within Pf1N1B4_3852 at 4274.181 kb on - strand, within Pf1N1B4_3852 at 4274.181 kb on - strand, within Pf1N1B4_3852 at 4274.214 kb on - strand, within Pf1N1B4_3852 at 4274.214 kb on - strand, within Pf1N1B4_3852 at 4274.270 kb on + strand, within Pf1N1B4_3852 at 4274.363 kb on + strand, within Pf1N1B4_3852
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source nitrite 0.5mM remove 4,271,853 - Pf1N1B4_3850 0.40 -0.3 4,271,861 + Pf1N1B4_3850 0.41 -2.0 4,271,862 - Pf1N1B4_3850 0.41 +0.1 4,271,862 - -0.6 4,271,948 + Pf1N1B4_3850 0.47 -0.3 4,271,949 - Pf1N1B4_3850 0.48 +0.4 4,271,949 - Pf1N1B4_3850 0.48 -1.8 4,271,949 - Pf1N1B4_3850 0.48 +0.2 4,271,949 - Pf1N1B4_3850 0.48 -2.7 4,271,949 - Pf1N1B4_3850 0.48 -0.4 4,272,089 + Pf1N1B4_3850 0.58 -2.9 4,272,090 - Pf1N1B4_3850 0.58 -1.9 4,272,090 - Pf1N1B4_3850 0.58 +0.5 4,272,090 - Pf1N1B4_3850 0.58 -0.2 4,272,117 - -0.9 4,272,117 - -0.3 4,272,332 + Pf1N1B4_3850 0.77 +0.4 4,272,332 + Pf1N1B4_3850 0.77 +1.1 4,272,332 + Pf1N1B4_3850 0.77 -2.0 4,272,332 + Pf1N1B4_3850 0.77 -0.4 4,272,332 + Pf1N1B4_3850 0.77 -1.6 4,272,333 - Pf1N1B4_3850 0.77 +0.3 4,272,333 - Pf1N1B4_3850 0.77 +1.1 4,272,333 - Pf1N1B4_3850 0.77 -0.3 4,272,333 - Pf1N1B4_3850 0.77 -1.0 4,272,333 - Pf1N1B4_3850 0.77 -1.1 4,272,333 - +1.7 4,272,333 - Pf1N1B4_3850 0.77 -3.1 4,272,352 + Pf1N1B4_3850 0.78 -1.2 4,272,352 + Pf1N1B4_3850 0.78 -2.4 4,272,352 + Pf1N1B4_3850 0.78 +0.0 4,272,352 + Pf1N1B4_3850 0.78 -2.0 4,272,366 - Pf1N1B4_3850 0.79 -0.0 4,272,551 + -1.4 4,272,647 + -0.7 4,272,647 + -0.3 4,272,648 - -1.3 4,272,766 + Pf1N1B4_3851 0.11 -1.4 4,272,766 + Pf1N1B4_3851 0.11 -1.4 4,272,766 + +0.1 4,272,766 + Pf1N1B4_3851 0.11 -0.3 4,272,766 + Pf1N1B4_3851 0.11 -0.9 4,272,766 + Pf1N1B4_3851 0.11 -2.1 4,272,766 + Pf1N1B4_3851 0.11 +1.0 4,272,766 + Pf1N1B4_3851 0.11 -0.3 4,272,767 - Pf1N1B4_3851 0.11 -0.5 4,272,767 - Pf1N1B4_3851 0.11 -2.8 4,273,021 + Pf1N1B4_3851 0.40 +0.4 4,273,021 + Pf1N1B4_3851 0.40 +2.3 4,273,022 - Pf1N1B4_3851 0.41 +0.7 4,273,354 + Pf1N1B4_3851 0.79 -1.5 4,273,354 + Pf1N1B4_3851 0.79 -0.1 4,273,354 + Pf1N1B4_3851 0.79 -1.3 4,273,355 - Pf1N1B4_3851 0.80 -0.3 4,273,355 - Pf1N1B4_3851 0.80 +0.9 4,273,516 + -1.0 4,273,516 + +0.0 4,273,516 + -0.5 4,273,516 + +1.0 4,273,516 + -0.6 4,273,516 + +1.7 4,273,517 - -0.2 4,273,517 - -0.3 4,273,517 - -0.8 4,273,517 - +0.4 4,273,517 - -0.1 4,273,517 - +0.3 4,273,517 - +0.5 4,273,517 - -0.5 4,273,517 - +1.6 4,273,952 + Pf1N1B4_3852 0.12 -1.2 4,273,953 - Pf1N1B4_3852 0.12 +0.3 4,273,953 - Pf1N1B4_3852 0.12 +0.7 4,274,120 + Pf1N1B4_3852 0.19 +0.1 4,274,175 - Pf1N1B4_3852 0.22 +0.6 4,274,177 + Pf1N1B4_3852 0.22 +1.5 4,274,178 - -2.1 4,274,178 - +0.8 4,274,178 - -1.4 4,274,178 - Pf1N1B4_3852 0.22 +0.9 4,274,178 - Pf1N1B4_3852 0.22 -1.3 4,274,180 + Pf1N1B4_3852 0.22 -0.5 4,274,181 - Pf1N1B4_3852 0.22 -0.3 4,274,181 - Pf1N1B4_3852 0.22 -0.5 4,274,214 - Pf1N1B4_3852 0.23 +0.2 4,274,214 - Pf1N1B4_3852 0.23 -0.8 4,274,270 + Pf1N1B4_3852 0.26 +2.6 4,274,363 + Pf1N1B4_3852 0.29 -0.4
Or see this region's nucleotide sequence