Experiment: nitrogen source nitrite 0.5mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3741 and Pf1N1B4_3742 are separated by 105 nucleotides Pf1N1B4_3742 and Pf1N1B4_3743 are separated by 168 nucleotides Pf1N1B4_3743 and Pf1N1B4_3744 are separated by 112 nucleotides
Pf1N1B4_3741 - Periplasmic protease, at 4,151,576 to 4,153,690
_3741
Pf1N1B4_3742 - Oxidoreductase, zinc-binding, at 4,153,796 to 4,154,758
_3742
Pf1N1B4_3743 - Phosphoserine phosphatase (EC 3.1.3.3), at 4,154,927 to 4,155,580
_3743
Pf1N1B4_3744 - ABC transporter, ATP-binding protein, at 4,155,693 to 4,156,682
_3744
Position (kb)
4153
4154
4155 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4153.852 kb on + strand at 4153.852 kb on + strand at 4153.852 kb on + strand at 4153.853 kb on - strand at 4153.853 kb on - strand at 4153.864 kb on - strand at 4153.962 kb on + strand, within Pf1N1B4_3742 at 4154.073 kb on + strand, within Pf1N1B4_3742 at 4154.073 kb on + strand, within Pf1N1B4_3742 at 4154.074 kb on - strand, within Pf1N1B4_3742 at 4154.521 kb on - strand, within Pf1N1B4_3742 at 4154.710 kb on + strand at 4154.710 kb on + strand at 4154.710 kb on + strand at 4154.929 kb on - strand at 4155.052 kb on - strand, within Pf1N1B4_3743 at 4155.126 kb on + strand, within Pf1N1B4_3743 at 4155.146 kb on - strand, within Pf1N1B4_3743 at 4155.411 kb on + strand at 4155.411 kb on + strand, within Pf1N1B4_3743 at 4155.411 kb on + strand, within Pf1N1B4_3743 at 4155.412 kb on - strand, within Pf1N1B4_3743 at 4155.412 kb on - strand, within Pf1N1B4_3743 at 4155.412 kb on - strand, within Pf1N1B4_3743 at 4155.412 kb on - strand, within Pf1N1B4_3743 at 4155.412 kb on - strand, within Pf1N1B4_3743 at 4155.412 kb on - strand at 4155.412 kb on - strand, within Pf1N1B4_3743 at 4155.412 kb on - strand, within Pf1N1B4_3743 at 4155.412 kb on - strand, within Pf1N1B4_3743 at 4155.441 kb on + strand at 4155.441 kb on + strand, within Pf1N1B4_3743 at 4155.442 kb on - strand, within Pf1N1B4_3743 at 4155.442 kb on - strand, within Pf1N1B4_3743 at 4155.655 kb on - strand at 4155.655 kb on - strand at 4155.677 kb on + strand at 4155.678 kb on - strand at 4155.678 kb on - strand at 4155.678 kb on - strand at 4155.678 kb on - strand at 4155.678 kb on - strand at 4155.678 kb on - strand at 4155.678 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source nitrite 0.5mM remove 4,153,852 + +0.1 4,153,852 + -0.1 4,153,852 + +2.1 4,153,853 - -1.7 4,153,853 - +0.7 4,153,864 - +0.1 4,153,962 + Pf1N1B4_3742 0.17 -0.1 4,154,073 + Pf1N1B4_3742 0.29 +0.6 4,154,073 + Pf1N1B4_3742 0.29 -3.1 4,154,074 - Pf1N1B4_3742 0.29 -0.9 4,154,521 - Pf1N1B4_3742 0.75 -1.9 4,154,710 + -0.5 4,154,710 + +0.8 4,154,710 + +1.2 4,154,929 - -0.3 4,155,052 - Pf1N1B4_3743 0.19 -0.6 4,155,126 + Pf1N1B4_3743 0.30 -0.2 4,155,146 - Pf1N1B4_3743 0.33 -2.3 4,155,411 + +1.7 4,155,411 + Pf1N1B4_3743 0.74 +0.5 4,155,411 + Pf1N1B4_3743 0.74 +0.7 4,155,412 - Pf1N1B4_3743 0.74 +0.5 4,155,412 - Pf1N1B4_3743 0.74 +1.0 4,155,412 - Pf1N1B4_3743 0.74 -1.0 4,155,412 - Pf1N1B4_3743 0.74 -2.3 4,155,412 - Pf1N1B4_3743 0.74 +0.0 4,155,412 - +0.5 4,155,412 - Pf1N1B4_3743 0.74 -0.5 4,155,412 - Pf1N1B4_3743 0.74 -0.3 4,155,412 - Pf1N1B4_3743 0.74 -0.4 4,155,441 + +0.1 4,155,441 + Pf1N1B4_3743 0.79 +0.1 4,155,442 - Pf1N1B4_3743 0.79 -1.2 4,155,442 - Pf1N1B4_3743 0.79 -1.3 4,155,655 - +0.0 4,155,655 - +0.2 4,155,677 + +0.0 4,155,678 - +1.2 4,155,678 - -3.0 4,155,678 - -2.0 4,155,678 - +0.1 4,155,678 - +0.1 4,155,678 - -0.3 4,155,678 - +0.1
Or see this region's nucleotide sequence