Experiment: nitrogen source nitrite 0.5mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3295 and Pf1N1B4_5219 are separated by 333 nucleotides Pf1N1B4_5219 and Pf1N1B4_3296 are separated by 181 nucleotides Pf1N1B4_3296 and Pf1N1B4_3297 are separated by 314 nucleotides
Pf1N1B4_3295 - Filamentation induced by cAMP protein Fic, at 3,681,228 to 3,682,793
_3295
Pf1N1B4_5219 - tRNA-Gly-CCC, at 3,683,127 to 3,683,197
_5219
Pf1N1B4_3296 - (R)-3-hydroxydecanoyl-ACP:CoA transacylase PhaG (3-hydroxyacyl-CoA-acyl carrier protein transferase) (EC 2.4.1.-), at 3,683,379 to 3,684,263
_3296
Pf1N1B4_3297 - Ribosomal small subunit pseudouridine synthase A (EC 4.2.1.70), at 3,684,578 to 3,685,270
_3297
Position (kb)
3683
3684
3685 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3682.405 kb on + strand, within Pf1N1B4_3295 at 3682.405 kb on + strand, within Pf1N1B4_3295 at 3682.406 kb on - strand, within Pf1N1B4_3295 at 3682.406 kb on - strand, within Pf1N1B4_3295 at 3682.422 kb on + strand, within Pf1N1B4_3295 at 3682.432 kb on + strand, within Pf1N1B4_3295 at 3682.433 kb on - strand, within Pf1N1B4_3295 at 3682.510 kb on + strand, within Pf1N1B4_3295 at 3682.561 kb on - strand, within Pf1N1B4_3295 at 3682.561 kb on - strand, within Pf1N1B4_3295 at 3682.606 kb on + strand, within Pf1N1B4_3295 at 3682.607 kb on - strand, within Pf1N1B4_3295 at 3682.707 kb on + strand at 3682.707 kb on + strand at 3682.707 kb on + strand at 3682.707 kb on + strand at 3682.867 kb on - strand at 3682.879 kb on - strand at 3682.972 kb on - strand at 3683.078 kb on - strand at 3683.176 kb on - strand, within Pf1N1B4_5219 at 3683.185 kb on - strand, within Pf1N1B4_5219 at 3683.186 kb on + strand, within Pf1N1B4_5219 at 3683.186 kb on + strand, within Pf1N1B4_5219 at 3683.187 kb on - strand, within Pf1N1B4_5219 at 3683.187 kb on - strand, within Pf1N1B4_5219 at 3683.187 kb on - strand, within Pf1N1B4_5219 at 3683.187 kb on - strand, within Pf1N1B4_5219 at 3683.187 kb on - strand at 3683.187 kb on - strand at 3683.265 kb on + strand at 3683.265 kb on + strand at 3683.326 kb on + strand at 3683.411 kb on - strand at 3683.411 kb on - strand at 3683.411 kb on - strand at 3683.590 kb on + strand at 3683.590 kb on + strand, within Pf1N1B4_3296 at 3683.590 kb on + strand, within Pf1N1B4_3296 at 3683.591 kb on - strand, within Pf1N1B4_3296 at 3683.591 kb on - strand, within Pf1N1B4_3296 at 3683.653 kb on + strand, within Pf1N1B4_3296 at 3683.740 kb on + strand, within Pf1N1B4_3296 at 3683.740 kb on + strand, within Pf1N1B4_3296 at 3683.831 kb on - strand at 3683.851 kb on + strand, within Pf1N1B4_3296 at 3684.088 kb on + strand, within Pf1N1B4_3296 at 3684.173 kb on + strand, within Pf1N1B4_3296 at 3684.173 kb on + strand, within Pf1N1B4_3296 at 3684.173 kb on + strand, within Pf1N1B4_3296 at 3684.214 kb on + strand at 3684.234 kb on + strand at 3684.234 kb on + strand at 3684.234 kb on + strand at 3684.235 kb on - strand at 3684.235 kb on - strand at 3684.235 kb on - strand at 3684.235 kb on - strand at 3684.235 kb on - strand at 3684.235 kb on - strand at 3684.236 kb on + strand at 3684.236 kb on + strand at 3684.268 kb on + strand at 3684.269 kb on - strand at 3684.326 kb on + strand at 3684.326 kb on + strand at 3684.327 kb on - strand at 3684.332 kb on + strand at 3684.333 kb on - strand at 3684.333 kb on - strand at 3684.333 kb on - strand at 3684.391 kb on + strand at 3684.391 kb on + strand at 3684.391 kb on + strand at 3684.392 kb on - strand at 3684.392 kb on - strand at 3684.392 kb on - strand at 3684.393 kb on + strand at 3684.393 kb on + strand at 3684.393 kb on + strand at 3684.394 kb on - strand at 3684.394 kb on - strand at 3684.455 kb on + strand at 3684.455 kb on + strand at 3684.455 kb on + strand at 3684.455 kb on + strand at 3684.461 kb on - strand at 3684.533 kb on - strand at 3684.579 kb on + strand at 3684.580 kb on - strand at 3684.677 kb on + strand, within Pf1N1B4_3297 at 3684.713 kb on - strand, within Pf1N1B4_3297 at 3684.873 kb on + strand, within Pf1N1B4_3297 at 3684.873 kb on + strand, within Pf1N1B4_3297 at 3684.873 kb on + strand, within Pf1N1B4_3297 at 3684.873 kb on + strand, within Pf1N1B4_3297 at 3684.873 kb on + strand, within Pf1N1B4_3297 at 3684.874 kb on - strand, within Pf1N1B4_3297 at 3684.874 kb on - strand, within Pf1N1B4_3297 at 3684.874 kb on - strand, within Pf1N1B4_3297 at 3684.876 kb on + strand, within Pf1N1B4_3297 at 3684.876 kb on + strand, within Pf1N1B4_3297 at 3684.877 kb on - strand at 3684.877 kb on - strand, within Pf1N1B4_3297 at 3684.877 kb on - strand, within Pf1N1B4_3297 at 3684.877 kb on - strand, within Pf1N1B4_3297 at 3685.217 kb on + strand at 3685.217 kb on + strand at 3685.217 kb on + strand at 3685.217 kb on + strand at 3685.226 kb on - strand at 3685.226 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source nitrite 0.5mM remove 3,682,405 + Pf1N1B4_3295 0.75 -0.1 3,682,405 + Pf1N1B4_3295 0.75 +2.9 3,682,406 - Pf1N1B4_3295 0.75 -1.0 3,682,406 - Pf1N1B4_3295 0.75 -1.0 3,682,422 + Pf1N1B4_3295 0.76 -0.1 3,682,432 + Pf1N1B4_3295 0.77 +0.5 3,682,433 - Pf1N1B4_3295 0.77 -0.2 3,682,510 + Pf1N1B4_3295 0.82 -1.7 3,682,561 - Pf1N1B4_3295 0.85 +0.2 3,682,561 - Pf1N1B4_3295 0.85 +1.6 3,682,606 + Pf1N1B4_3295 0.88 -0.5 3,682,607 - Pf1N1B4_3295 0.88 -0.7 3,682,707 + +2.0 3,682,707 + -1.7 3,682,707 + +0.2 3,682,707 + +0.5 3,682,867 - -0.1 3,682,879 - -1.3 3,682,972 - -0.5 3,683,078 - +0.6 3,683,176 - Pf1N1B4_5219 0.69 -1.7 3,683,185 - Pf1N1B4_5219 0.82 -1.1 3,683,186 + Pf1N1B4_5219 0.83 -1.0 3,683,186 + Pf1N1B4_5219 0.83 -2.5 3,683,187 - Pf1N1B4_5219 0.85 +0.3 3,683,187 - Pf1N1B4_5219 0.85 +0.1 3,683,187 - Pf1N1B4_5219 0.85 -1.9 3,683,187 - Pf1N1B4_5219 0.85 +0.5 3,683,187 - -0.5 3,683,187 - +1.0 3,683,265 + -3.1 3,683,265 + +0.1 3,683,326 + -0.3 3,683,411 - +0.6 3,683,411 - -0.2 3,683,411 - -1.1 3,683,590 + -2.0 3,683,590 + Pf1N1B4_3296 0.24 -0.5 3,683,590 + Pf1N1B4_3296 0.24 -1.5 3,683,591 - Pf1N1B4_3296 0.24 -2.5 3,683,591 - Pf1N1B4_3296 0.24 -2.5 3,683,653 + Pf1N1B4_3296 0.31 -0.6 3,683,740 + Pf1N1B4_3296 0.41 -1.6 3,683,740 + Pf1N1B4_3296 0.41 -1.6 3,683,831 - +0.4 3,683,851 + Pf1N1B4_3296 0.53 -1.1 3,684,088 + Pf1N1B4_3296 0.80 -2.0 3,684,173 + Pf1N1B4_3296 0.90 -0.4 3,684,173 + Pf1N1B4_3296 0.90 -2.0 3,684,173 + Pf1N1B4_3296 0.90 -0.6 3,684,214 + -1.1 3,684,234 + +1.3 3,684,234 + -0.4 3,684,234 + -2.8 3,684,235 - +0.6 3,684,235 - -1.6 3,684,235 - -0.6 3,684,235 - -0.7 3,684,235 - +1.6 3,684,235 - -2.4 3,684,236 + -0.3 3,684,236 + -1.7 3,684,268 + +0.2 3,684,269 - -1.3 3,684,326 + -1.2 3,684,326 + -1.8 3,684,327 - -1.3 3,684,332 + -2.1 3,684,333 - -1.8 3,684,333 - -1.0 3,684,333 - -2.7 3,684,391 + -3.1 3,684,391 + -1.1 3,684,391 + -0.1 3,684,392 - -1.0 3,684,392 - -2.9 3,684,392 - -1.0 3,684,393 + +0.2 3,684,393 + -2.1 3,684,393 + -2.1 3,684,394 - +1.5 3,684,394 - +1.3 3,684,455 + +0.1 3,684,455 + -2.5 3,684,455 + -3.2 3,684,455 + -0.3 3,684,461 - -1.3 3,684,533 - -1.6 3,684,579 + -1.1 3,684,580 - +0.1 3,684,677 + Pf1N1B4_3297 0.14 +1.2 3,684,713 - Pf1N1B4_3297 0.19 -2.5 3,684,873 + Pf1N1B4_3297 0.43 -0.4 3,684,873 + Pf1N1B4_3297 0.43 -1.1 3,684,873 + Pf1N1B4_3297 0.43 -1.5 3,684,873 + Pf1N1B4_3297 0.43 +1.2 3,684,873 + Pf1N1B4_3297 0.43 -1.4 3,684,874 - Pf1N1B4_3297 0.43 +0.5 3,684,874 - Pf1N1B4_3297 0.43 -0.5 3,684,874 - Pf1N1B4_3297 0.43 -0.6 3,684,876 + Pf1N1B4_3297 0.43 -0.3 3,684,876 + Pf1N1B4_3297 0.43 -2.1 3,684,877 - +2.5 3,684,877 - Pf1N1B4_3297 0.43 -0.1 3,684,877 - Pf1N1B4_3297 0.43 +2.0 3,684,877 - Pf1N1B4_3297 0.43 -1.3 3,685,217 + +0.2 3,685,217 + -1.4 3,685,217 + +0.1 3,685,217 + -0.3 3,685,226 - -1.4 3,685,226 - -0.0
Or see this region's nucleotide sequence