Experiment: nitrogen source nitrite 0.5mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3174 and Pf1N1B4_3175 are separated by 92 nucleotides Pf1N1B4_3175 and Pf1N1B4_3176 are separated by 13 nucleotides Pf1N1B4_3176 and Pf1N1B4_3177 are separated by 12 nucleotides Pf1N1B4_3177 and Pf1N1B4_3178 overlap by 4 nucleotides
Pf1N1B4_3174 - Mobile element protein, at 3,556,095 to 3,557,123
_3174
Pf1N1B4_3175 - Histidine ABC transporter, ATP-binding protein HisP (TC 3.A.1.3.1), at 3,557,216 to 3,557,980
_3175
Pf1N1B4_3176 - Succinylglutamate desuccinylase/aspartoacylase, at 3,557,994 to 3,559,112
_3176
Pf1N1B4_3177 - Histidine ABC transporter, permease protein HisM (TC 3.A.1.3.1), at 3,559,125 to 3,559,835
_3177
Pf1N1B4_3178 - Histidine ABC transporter, permease protein HisQ (TC 3.A.1.3.1), at 3,559,832 to 3,560,560
_3178
Position (kb)
3557
3558
3559
3560 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3557.218 kb on - strand at 3557.218 kb on - strand at 3557.218 kb on - strand at 3557.273 kb on + strand at 3557.273 kb on + strand at 3557.273 kb on + strand at 3557.273 kb on + strand at 3557.273 kb on + strand at 3557.273 kb on + strand at 3557.274 kb on - strand at 3557.274 kb on - strand at 3557.274 kb on - strand at 3557.274 kb on - strand at 3557.274 kb on - strand at 3557.274 kb on - strand at 3557.586 kb on + strand, within Pf1N1B4_3175 at 3557.587 kb on - strand, within Pf1N1B4_3175 at 3557.943 kb on + strand at 3557.943 kb on + strand at 3557.944 kb on - strand at 3557.944 kb on - strand at 3557.950 kb on + strand at 3557.950 kb on + strand at 3557.951 kb on - strand at 3557.951 kb on - strand at 3557.976 kb on + strand at 3557.977 kb on - strand at 3557.977 kb on - strand at 3558.006 kb on + strand at 3558.006 kb on + strand at 3558.006 kb on + strand at 3558.006 kb on + strand at 3558.007 kb on - strand at 3558.007 kb on - strand at 3558.007 kb on - strand at 3558.007 kb on - strand at 3558.007 kb on - strand at 3558.007 kb on - strand at 3558.091 kb on + strand at 3558.091 kb on + strand at 3558.091 kb on + strand at 3558.092 kb on - strand at 3558.092 kb on - strand at 3558.092 kb on - strand at 3558.092 kb on - strand at 3558.092 kb on - strand at 3558.092 kb on - strand at 3558.181 kb on + strand, within Pf1N1B4_3176 at 3558.322 kb on + strand, within Pf1N1B4_3176 at 3558.323 kb on - strand, within Pf1N1B4_3176 at 3558.533 kb on - strand, within Pf1N1B4_3176 at 3558.577 kb on + strand, within Pf1N1B4_3176 at 3558.577 kb on + strand, within Pf1N1B4_3176 at 3558.577 kb on + strand, within Pf1N1B4_3176 at 3558.577 kb on + strand, within Pf1N1B4_3176 at 3558.578 kb on - strand, within Pf1N1B4_3176 at 3558.578 kb on - strand, within Pf1N1B4_3176 at 3558.735 kb on - strand, within Pf1N1B4_3176 at 3559.012 kb on + strand at 3559.012 kb on + strand at 3559.012 kb on + strand at 3559.012 kb on + strand at 3559.013 kb on - strand at 3559.013 kb on - strand at 3559.013 kb on - strand at 3559.114 kb on + strand at 3559.114 kb on + strand at 3559.114 kb on + strand at 3559.114 kb on + strand at 3559.114 kb on + strand at 3559.115 kb on - strand at 3559.115 kb on - strand at 3559.127 kb on - strand at 3559.207 kb on + strand, within Pf1N1B4_3177 at 3559.207 kb on + strand, within Pf1N1B4_3177 at 3559.208 kb on - strand, within Pf1N1B4_3177 at 3559.281 kb on + strand at 3559.281 kb on + strand, within Pf1N1B4_3177 at 3559.348 kb on + strand, within Pf1N1B4_3177 at 3559.348 kb on + strand, within Pf1N1B4_3177 at 3559.348 kb on + strand, within Pf1N1B4_3177 at 3559.348 kb on + strand, within Pf1N1B4_3177 at 3559.348 kb on + strand, within Pf1N1B4_3177 at 3559.349 kb on - strand, within Pf1N1B4_3177 at 3559.349 kb on - strand, within Pf1N1B4_3177 at 3559.351 kb on + strand, within Pf1N1B4_3177 at 3559.351 kb on + strand, within Pf1N1B4_3177 at 3559.351 kb on + strand, within Pf1N1B4_3177 at 3559.351 kb on + strand, within Pf1N1B4_3177 at 3559.352 kb on - strand, within Pf1N1B4_3177 at 3559.390 kb on + strand, within Pf1N1B4_3177 at 3559.390 kb on + strand, within Pf1N1B4_3177 at 3559.391 kb on - strand, within Pf1N1B4_3177 at 3559.391 kb on - strand, within Pf1N1B4_3177 at 3559.397 kb on - strand, within Pf1N1B4_3177 at 3559.397 kb on - strand, within Pf1N1B4_3177 at 3559.420 kb on + strand, within Pf1N1B4_3177 at 3559.489 kb on + strand, within Pf1N1B4_3177 at 3559.489 kb on + strand, within Pf1N1B4_3177 at 3559.588 kb on + strand, within Pf1N1B4_3177 at 3559.588 kb on + strand, within Pf1N1B4_3177 at 3559.588 kb on + strand, within Pf1N1B4_3177 at 3559.589 kb on - strand, within Pf1N1B4_3177 at 3559.589 kb on - strand, within Pf1N1B4_3177 at 3559.600 kb on + strand, within Pf1N1B4_3177 at 3559.601 kb on - strand, within Pf1N1B4_3177 at 3559.620 kb on - strand, within Pf1N1B4_3177 at 3559.621 kb on + strand, within Pf1N1B4_3177 at 3559.621 kb on + strand, within Pf1N1B4_3177 at 3559.621 kb on + strand, within Pf1N1B4_3177 at 3559.621 kb on + strand, within Pf1N1B4_3177 at 3559.621 kb on + strand, within Pf1N1B4_3177 at 3559.621 kb on + strand, within Pf1N1B4_3177 at 3559.621 kb on + strand, within Pf1N1B4_3177 at 3559.621 kb on + strand, within Pf1N1B4_3177 at 3559.621 kb on + strand, within Pf1N1B4_3177 at 3559.622 kb on - strand, within Pf1N1B4_3177 at 3559.622 kb on - strand, within Pf1N1B4_3177 at 3559.622 kb on - strand at 3559.622 kb on - strand, within Pf1N1B4_3177 at 3559.622 kb on - strand, within Pf1N1B4_3177 at 3559.645 kb on + strand, within Pf1N1B4_3177 at 3559.645 kb on + strand, within Pf1N1B4_3177 at 3559.646 kb on - strand, within Pf1N1B4_3177 at 3559.646 kb on - strand, within Pf1N1B4_3177 at 3559.646 kb on - strand, within Pf1N1B4_3177 at 3559.646 kb on - strand, within Pf1N1B4_3177 at 3559.646 kb on - strand, within Pf1N1B4_3177 at 3559.651 kb on + strand, within Pf1N1B4_3177 at 3559.651 kb on + strand, within Pf1N1B4_3177 at 3559.651 kb on + strand, within Pf1N1B4_3177 at 3559.651 kb on + strand, within Pf1N1B4_3177 at 3559.651 kb on + strand, within Pf1N1B4_3177 at 3559.651 kb on + strand, within Pf1N1B4_3177 at 3559.652 kb on - strand, within Pf1N1B4_3177 at 3559.652 kb on - strand, within Pf1N1B4_3177 at 3559.652 kb on - strand, within Pf1N1B4_3177 at 3559.675 kb on + strand, within Pf1N1B4_3177 at 3559.675 kb on + strand, within Pf1N1B4_3177 at 3559.675 kb on + strand, within Pf1N1B4_3177 at 3559.675 kb on + strand, within Pf1N1B4_3177 at 3559.675 kb on + strand, within Pf1N1B4_3177 at 3559.676 kb on - strand, within Pf1N1B4_3177 at 3559.676 kb on - strand, within Pf1N1B4_3177 at 3559.676 kb on - strand at 3559.676 kb on - strand, within Pf1N1B4_3177 at 3559.783 kb on + strand at 3559.793 kb on + strand at 3559.794 kb on - strand at 3559.796 kb on - strand at 3559.796 kb on - strand at 3559.796 kb on - strand at 3559.813 kb on + strand at 3559.813 kb on + strand at 3559.945 kb on - strand, within Pf1N1B4_3178 at 3559.945 kb on - strand, within Pf1N1B4_3178 at 3559.956 kb on + strand, within Pf1N1B4_3178 at 3559.956 kb on + strand, within Pf1N1B4_3178 at 3559.956 kb on + strand, within Pf1N1B4_3178 at 3559.957 kb on - strand, within Pf1N1B4_3178 at 3559.957 kb on - strand, within Pf1N1B4_3178 at 3559.957 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source nitrite 0.5mM remove 3,557,218 - -1.8 3,557,218 - +1.7 3,557,218 - +0.6 3,557,273 + +0.6 3,557,273 + +0.5 3,557,273 + -2.2 3,557,273 + -0.5 3,557,273 + -0.2 3,557,273 + +1.2 3,557,274 - +1.3 3,557,274 - -1.9 3,557,274 - -1.1 3,557,274 - -1.8 3,557,274 - +0.1 3,557,274 - +0.2 3,557,586 + Pf1N1B4_3175 0.48 -2.6 3,557,587 - Pf1N1B4_3175 0.48 +0.9 3,557,943 + -0.7 3,557,943 + -0.1 3,557,944 - -0.1 3,557,944 - -0.1 3,557,950 + -0.6 3,557,950 + +0.1 3,557,951 - -0.6 3,557,951 - +0.6 3,557,976 + -2.7 3,557,977 - -1.6 3,557,977 - -0.6 3,558,006 + -0.3 3,558,006 + +1.6 3,558,006 + -0.7 3,558,006 + -1.0 3,558,007 - -1.1 3,558,007 - +0.1 3,558,007 - +0.9 3,558,007 - +0.1 3,558,007 - +0.8 3,558,007 - -2.3 3,558,091 + +0.6 3,558,091 + -2.5 3,558,091 + -0.5 3,558,092 - +0.4 3,558,092 - +0.7 3,558,092 - +0.2 3,558,092 - +0.4 3,558,092 - -2.4 3,558,092 - +0.8 3,558,181 + Pf1N1B4_3176 0.17 -0.7 3,558,322 + Pf1N1B4_3176 0.29 -1.2 3,558,323 - Pf1N1B4_3176 0.29 +0.1 3,558,533 - Pf1N1B4_3176 0.48 +0.2 3,558,577 + Pf1N1B4_3176 0.52 -0.8 3,558,577 + Pf1N1B4_3176 0.52 -0.8 3,558,577 + Pf1N1B4_3176 0.52 -2.3 3,558,577 + Pf1N1B4_3176 0.52 +0.9 3,558,578 - Pf1N1B4_3176 0.52 -0.6 3,558,578 - Pf1N1B4_3176 0.52 -0.8 3,558,735 - Pf1N1B4_3176 0.66 -0.4 3,559,012 + -2.7 3,559,012 + -2.9 3,559,012 + -0.5 3,559,012 + -0.6 3,559,013 - +0.3 3,559,013 - +0.6 3,559,013 - +1.5 3,559,114 + -0.8 3,559,114 + -0.1 3,559,114 + -1.6 3,559,114 + -0.3 3,559,114 + -0.4 3,559,115 - -1.7 3,559,115 - -0.4 3,559,127 - -0.6 3,559,207 + Pf1N1B4_3177 0.12 +0.1 3,559,207 + Pf1N1B4_3177 0.12 +1.8 3,559,208 - Pf1N1B4_3177 0.12 +0.2 3,559,281 + -1.6 3,559,281 + Pf1N1B4_3177 0.22 +1.6 3,559,348 + Pf1N1B4_3177 0.31 +1.4 3,559,348 + Pf1N1B4_3177 0.31 -0.3 3,559,348 + Pf1N1B4_3177 0.31 +0.6 3,559,348 + Pf1N1B4_3177 0.31 +1.0 3,559,348 + Pf1N1B4_3177 0.31 +0.1 3,559,349 - Pf1N1B4_3177 0.32 -0.1 3,559,349 - Pf1N1B4_3177 0.32 -0.6 3,559,351 + Pf1N1B4_3177 0.32 -0.5 3,559,351 + Pf1N1B4_3177 0.32 +1.0 3,559,351 + Pf1N1B4_3177 0.32 -0.7 3,559,351 + Pf1N1B4_3177 0.32 +0.9 3,559,352 - Pf1N1B4_3177 0.32 +0.3 3,559,390 + Pf1N1B4_3177 0.37 +0.3 3,559,390 + Pf1N1B4_3177 0.37 +0.3 3,559,391 - Pf1N1B4_3177 0.37 +0.8 3,559,391 - Pf1N1B4_3177 0.37 +0.0 3,559,397 - Pf1N1B4_3177 0.38 -0.2 3,559,397 - Pf1N1B4_3177 0.38 -0.3 3,559,420 + Pf1N1B4_3177 0.41 -1.0 3,559,489 + Pf1N1B4_3177 0.51 -2.0 3,559,489 + Pf1N1B4_3177 0.51 -0.7 3,559,588 + Pf1N1B4_3177 0.65 -0.1 3,559,588 + Pf1N1B4_3177 0.65 -0.8 3,559,588 + Pf1N1B4_3177 0.65 -1.3 3,559,589 - Pf1N1B4_3177 0.65 +1.2 3,559,589 - Pf1N1B4_3177 0.65 +2.6 3,559,600 + Pf1N1B4_3177 0.67 -1.3 3,559,601 - Pf1N1B4_3177 0.67 -1.8 3,559,620 - Pf1N1B4_3177 0.70 +0.0 3,559,621 + Pf1N1B4_3177 0.70 -2.7 3,559,621 + Pf1N1B4_3177 0.70 +0.7 3,559,621 + Pf1N1B4_3177 0.70 +0.2 3,559,621 + Pf1N1B4_3177 0.70 -1.3 3,559,621 + Pf1N1B4_3177 0.70 +0.4 3,559,621 + Pf1N1B4_3177 0.70 +0.6 3,559,621 + Pf1N1B4_3177 0.70 -1.9 3,559,621 + Pf1N1B4_3177 0.70 +0.3 3,559,621 + Pf1N1B4_3177 0.70 -1.9 3,559,622 - Pf1N1B4_3177 0.70 -1.1 3,559,622 - Pf1N1B4_3177 0.70 -2.0 3,559,622 - -2.3 3,559,622 - Pf1N1B4_3177 0.70 -2.6 3,559,622 - Pf1N1B4_3177 0.70 +2.2 3,559,645 + Pf1N1B4_3177 0.73 -0.3 3,559,645 + Pf1N1B4_3177 0.73 -2.1 3,559,646 - Pf1N1B4_3177 0.73 +0.9 3,559,646 - Pf1N1B4_3177 0.73 -1.3 3,559,646 - Pf1N1B4_3177 0.73 -0.7 3,559,646 - Pf1N1B4_3177 0.73 +0.5 3,559,646 - Pf1N1B4_3177 0.73 +1.2 3,559,651 + Pf1N1B4_3177 0.74 -0.6 3,559,651 + Pf1N1B4_3177 0.74 -1.7 3,559,651 + Pf1N1B4_3177 0.74 +0.2 3,559,651 + Pf1N1B4_3177 0.74 +1.3 3,559,651 + Pf1N1B4_3177 0.74 +0.6 3,559,651 + Pf1N1B4_3177 0.74 -1.6 3,559,652 - Pf1N1B4_3177 0.74 -1.2 3,559,652 - Pf1N1B4_3177 0.74 +0.5 3,559,652 - Pf1N1B4_3177 0.74 +2.0 3,559,675 + Pf1N1B4_3177 0.77 -1.1 3,559,675 + Pf1N1B4_3177 0.77 +0.6 3,559,675 + Pf1N1B4_3177 0.77 +2.7 3,559,675 + Pf1N1B4_3177 0.77 -0.4 3,559,675 + Pf1N1B4_3177 0.77 +0.1 3,559,676 - Pf1N1B4_3177 0.77 -0.0 3,559,676 - Pf1N1B4_3177 0.77 -1.8 3,559,676 - +0.7 3,559,676 - Pf1N1B4_3177 0.77 -0.2 3,559,783 + -0.4 3,559,793 + +0.2 3,559,794 - +1.2 3,559,796 - -2.6 3,559,796 - -0.6 3,559,796 - -0.1 3,559,813 + +1.5 3,559,813 + -0.9 3,559,945 - Pf1N1B4_3178 0.16 -1.5 3,559,945 - Pf1N1B4_3178 0.16 -0.6 3,559,956 + Pf1N1B4_3178 0.17 +0.9 3,559,956 + Pf1N1B4_3178 0.17 +0.4 3,559,956 + Pf1N1B4_3178 0.17 +0.3 3,559,957 - Pf1N1B4_3178 0.17 -0.9 3,559,957 - Pf1N1B4_3178 0.17 -0.0 3,559,957 - +0.5
Or see this region's nucleotide sequence