Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_311

Experiment: nitrogen source nitrite 0.5mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_310 and Pf1N1B4_311 are separated by 141 nucleotidesPf1N1B4_311 and Pf1N1B4_312 are separated by 48 nucleotides Pf1N1B4_310 - UPF0028 protein YchK, at 363,216 to 364,256 _310 Pf1N1B4_311 - sensor histidine kinase, at 364,398 to 365,096 _311 Pf1N1B4_312 - Osmosensitive K+ channel histidine kinase KdpD (EC 2.7.3.-), at 365,145 to 367,796 _312 Position (kb) 364 365 366Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 363.411 kb on + strand, within Pf1N1B4_310at 363.509 kb on + strand, within Pf1N1B4_310at 363.509 kb on + strand, within Pf1N1B4_310at 363.510 kb on - strand, within Pf1N1B4_310at 363.510 kb on - strand, within Pf1N1B4_310at 363.512 kb on + strand, within Pf1N1B4_310at 363.512 kb on + strand, within Pf1N1B4_310at 363.512 kb on + strand, within Pf1N1B4_310at 363.771 kb on + strand, within Pf1N1B4_310at 363.771 kb on + strandat 364.101 kb on + strand, within Pf1N1B4_310at 364.102 kb on - strand, within Pf1N1B4_310at 364.173 kb on + strandat 364.173 kb on + strandat 364.174 kb on - strandat 364.174 kb on - strandat 364.174 kb on - strandat 364.174 kb on - strandat 364.174 kb on - strandat 364.234 kb on - strandat 364.399 kb on + strandat 364.399 kb on + strandat 364.399 kb on + strandat 364.451 kb on + strandat 364.506 kb on + strand, within Pf1N1B4_311at 364.516 kb on + strand, within Pf1N1B4_311at 364.516 kb on + strand, within Pf1N1B4_311at 364.516 kb on + strand, within Pf1N1B4_311at 364.516 kb on + strand, within Pf1N1B4_311at 364.618 kb on + strand, within Pf1N1B4_311at 364.618 kb on + strand, within Pf1N1B4_311at 364.618 kb on + strand, within Pf1N1B4_311at 364.618 kb on + strand, within Pf1N1B4_311at 364.619 kb on - strand, within Pf1N1B4_311at 364.795 kb on + strand, within Pf1N1B4_311at 364.795 kb on + strand, within Pf1N1B4_311at 364.795 kb on + strand, within Pf1N1B4_311at 364.795 kb on + strand, within Pf1N1B4_311at 364.796 kb on - strand, within Pf1N1B4_311at 364.796 kb on - strand, within Pf1N1B4_311at 364.796 kb on - strand, within Pf1N1B4_311at 364.824 kb on + strand, within Pf1N1B4_311at 364.824 kb on + strand, within Pf1N1B4_311at 365.105 kb on - strandat 365.131 kb on + strandat 365.326 kb on + strandat 365.326 kb on + strandat 365.326 kb on + strandat 365.327 kb on - strandat 365.512 kb on + strand, within Pf1N1B4_312at 365.512 kb on + strand, within Pf1N1B4_312at 365.512 kb on + strand, within Pf1N1B4_312at 365.512 kb on + strand, within Pf1N1B4_312at 365.512 kb on + strandat 365.512 kb on + strand, within Pf1N1B4_312at 365.512 kb on + strand, within Pf1N1B4_312at 365.512 kb on + strand, within Pf1N1B4_312at 365.512 kb on + strand, within Pf1N1B4_312at 365.512 kb on + strand, within Pf1N1B4_312at 365.512 kb on + strand, within Pf1N1B4_312at 365.512 kb on + strand, within Pf1N1B4_312at 365.512 kb on + strand, within Pf1N1B4_312at 365.512 kb on + strand, within Pf1N1B4_312at 365.512 kb on + strand, within Pf1N1B4_312at 365.512 kb on + strand, within Pf1N1B4_312at 365.513 kb on - strand, within Pf1N1B4_312at 365.513 kb on - strand, within Pf1N1B4_312at 365.513 kb on - strand, within Pf1N1B4_312at 365.513 kb on - strand, within Pf1N1B4_312at 365.513 kb on - strand, within Pf1N1B4_312at 365.513 kb on - strand, within Pf1N1B4_312at 365.513 kb on - strand, within Pf1N1B4_312at 365.664 kb on + strand, within Pf1N1B4_312at 365.665 kb on - strand, within Pf1N1B4_312at 365.665 kb on - strand, within Pf1N1B4_312at 365.665 kb on - strand, within Pf1N1B4_312at 365.679 kb on - strand, within Pf1N1B4_312at 365.679 kb on - strand, within Pf1N1B4_312at 365.680 kb on + strand, within Pf1N1B4_312at 365.680 kb on + strand, within Pf1N1B4_312at 365.680 kb on + strand, within Pf1N1B4_312at 365.680 kb on + strand, within Pf1N1B4_312at 365.681 kb on - strand, within Pf1N1B4_312at 365.808 kb on + strand, within Pf1N1B4_312at 365.809 kb on - strand, within Pf1N1B4_312

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Per-strain Table

Position Strand Gene LocusTag Fraction nitrogen source nitrite 0.5mM
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363,411 + Pf1N1B4_310 0.19 -0.6
363,509 + Pf1N1B4_310 0.28 -0.8
363,509 + Pf1N1B4_310 0.28 +0.6
363,510 - Pf1N1B4_310 0.28 +0.5
363,510 - Pf1N1B4_310 0.28 -0.3
363,512 + Pf1N1B4_310 0.28 -0.4
363,512 + Pf1N1B4_310 0.28 +0.0
363,512 + Pf1N1B4_310 0.28 +0.2
363,771 + Pf1N1B4_310 0.53 -2.6
363,771 + -1.2
364,101 + Pf1N1B4_310 0.85 -0.2
364,102 - Pf1N1B4_310 0.85 -0.3
364,173 + -0.1
364,173 + -0.5
364,174 - +0.1
364,174 - -0.5
364,174 - -1.0
364,174 - -1.2
364,174 - -0.4
364,234 - +0.0
364,399 + -0.1
364,399 + +0.2
364,399 + -1.0
364,451 + +0.0
364,506 + Pf1N1B4_311 0.15 -0.4
364,516 + Pf1N1B4_311 0.17 -1.1
364,516 + Pf1N1B4_311 0.17 -1.0
364,516 + Pf1N1B4_311 0.17 +0.3
364,516 + Pf1N1B4_311 0.17 -1.6
364,618 + Pf1N1B4_311 0.31 -1.2
364,618 + Pf1N1B4_311 0.31 -2.3
364,618 + Pf1N1B4_311 0.31 +0.3
364,618 + Pf1N1B4_311 0.31 +1.9
364,619 - Pf1N1B4_311 0.32 +1.0
364,795 + Pf1N1B4_311 0.57 -0.8
364,795 + Pf1N1B4_311 0.57 -2.4
364,795 + Pf1N1B4_311 0.57 -2.8
364,795 + Pf1N1B4_311 0.57 +0.9
364,796 - Pf1N1B4_311 0.57 -2.3
364,796 - Pf1N1B4_311 0.57 -1.7
364,796 - Pf1N1B4_311 0.57 +0.3
364,824 + Pf1N1B4_311 0.61 -1.0
364,824 + Pf1N1B4_311 0.61 -1.2
365,105 - -0.5
365,131 + -0.2
365,326 + -1.7
365,326 + +0.3
365,326 + +0.5
365,327 - +0.7
365,512 + Pf1N1B4_312 0.14 +0.3
365,512 + Pf1N1B4_312 0.14 -1.3
365,512 + Pf1N1B4_312 0.14 -1.7
365,512 + Pf1N1B4_312 0.14 +0.6
365,512 + -1.0
365,512 + Pf1N1B4_312 0.14 +0.8
365,512 + Pf1N1B4_312 0.14 +1.1
365,512 + Pf1N1B4_312 0.14 +1.2
365,512 + Pf1N1B4_312 0.14 -2.4
365,512 + Pf1N1B4_312 0.14 -0.7
365,512 + Pf1N1B4_312 0.14 +0.5
365,512 + Pf1N1B4_312 0.14 -2.9
365,512 + Pf1N1B4_312 0.14 +0.6
365,512 + Pf1N1B4_312 0.14 +2.0
365,512 + Pf1N1B4_312 0.14 -0.9
365,512 + Pf1N1B4_312 0.14 -0.8
365,513 - Pf1N1B4_312 0.14 -1.1
365,513 - Pf1N1B4_312 0.14 +0.4
365,513 - Pf1N1B4_312 0.14 +0.8
365,513 - Pf1N1B4_312 0.14 -1.1
365,513 - Pf1N1B4_312 0.14 +1.3
365,513 - Pf1N1B4_312 0.14 +1.2
365,513 - Pf1N1B4_312 0.14 -0.2
365,664 + Pf1N1B4_312 0.20 -1.8
365,665 - Pf1N1B4_312 0.20 +0.8
365,665 - Pf1N1B4_312 0.20 +1.4
365,665 - Pf1N1B4_312 0.20 -0.2
365,679 - Pf1N1B4_312 0.20 +0.9
365,679 - Pf1N1B4_312 0.20 +1.0
365,680 + Pf1N1B4_312 0.20 +0.6
365,680 + Pf1N1B4_312 0.20 -1.6
365,680 + Pf1N1B4_312 0.20 +0.2
365,680 + Pf1N1B4_312 0.20 +0.6
365,681 - Pf1N1B4_312 0.20 -0.9
365,808 + Pf1N1B4_312 0.25 -0.0
365,809 - Pf1N1B4_312 0.25 +0.1

Or see this region's nucleotide sequence