Experiment: nitrogen source nitrite 0.5mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2165 and Pf1N1B4_2166 are separated by 418 nucleotides Pf1N1B4_2166 and Pf1N1B4_2167 are separated by 35 nucleotides Pf1N1B4_2167 and Pf1N1B4_2168 are separated by 214 nucleotides Pf1N1B4_2168 and Pf1N1B4_2169 are separated by 104 nucleotides
Pf1N1B4_2165 - FIG00953456: hypothetical protein, at 2,479,994 to 2,480,260
_2165
Pf1N1B4_2166 - Nitrogen regulatory protein P-II, glnK, at 2,480,679 to 2,481,017
_2166
Pf1N1B4_2167 - Ammonium transporter, at 2,481,053 to 2,482,390
_2167
Pf1N1B4_2168 - FIG004064: hypothetical protein, at 2,482,605 to 2,483,030
_2168
Pf1N1B4_2169 - FIG00953808: hypothetical protein, at 2,483,135 to 2,483,458
_2169
Position (kb)
2481
2482
2483 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2480.294 kb on + strand at 2480.294 kb on + strand at 2480.294 kb on + strand at 2480.294 kb on + strand at 2480.294 kb on + strand at 2480.294 kb on + strand at 2480.294 kb on + strand at 2480.295 kb on - strand at 2480.295 kb on - strand at 2480.308 kb on + strand at 2480.308 kb on + strand at 2480.308 kb on + strand at 2480.308 kb on + strand at 2480.308 kb on + strand at 2480.308 kb on + strand at 2480.308 kb on + strand at 2480.308 kb on + strand at 2480.308 kb on + strand at 2480.308 kb on + strand at 2480.308 kb on + strand at 2480.309 kb on - strand at 2480.309 kb on - strand at 2480.310 kb on + strand at 2480.310 kb on + strand at 2480.311 kb on - strand at 2480.453 kb on - strand at 2480.560 kb on - strand at 2480.620 kb on + strand at 2480.620 kb on + strand at 2480.620 kb on + strand at 2481.015 kb on + strand at 2481.016 kb on - strand at 2481.052 kb on + strand at 2481.052 kb on + strand at 2481.052 kb on + strand at 2481.052 kb on + strand at 2481.053 kb on - strand at 2481.078 kb on + strand at 2481.228 kb on - strand, within Pf1N1B4_2167 at 2481.317 kb on + strand, within Pf1N1B4_2167 at 2481.317 kb on + strand, within Pf1N1B4_2167 at 2481.317 kb on + strand, within Pf1N1B4_2167 at 2481.317 kb on + strand, within Pf1N1B4_2167 at 2481.317 kb on + strand, within Pf1N1B4_2167 at 2481.318 kb on - strand, within Pf1N1B4_2167 at 2481.318 kb on - strand, within Pf1N1B4_2167 at 2481.323 kb on + strand, within Pf1N1B4_2167 at 2481.587 kb on + strand at 2481.587 kb on + strand, within Pf1N1B4_2167 at 2481.587 kb on + strand, within Pf1N1B4_2167 at 2481.587 kb on + strand, within Pf1N1B4_2167 at 2481.588 kb on - strand, within Pf1N1B4_2167 at 2481.588 kb on - strand, within Pf1N1B4_2167 at 2481.588 kb on - strand, within Pf1N1B4_2167 at 2481.773 kb on + strand, within Pf1N1B4_2167 at 2481.774 kb on - strand, within Pf1N1B4_2167 at 2481.870 kb on + strand, within Pf1N1B4_2167 at 2481.870 kb on + strand, within Pf1N1B4_2167 at 2481.870 kb on + strand, within Pf1N1B4_2167 at 2481.871 kb on - strand, within Pf1N1B4_2167 at 2481.871 kb on - strand, within Pf1N1B4_2167 at 2482.262 kb on + strand at 2482.262 kb on + strand at 2482.262 kb on + strand at 2482.262 kb on + strand at 2482.262 kb on + strand at 2482.263 kb on - strand at 2482.263 kb on - strand at 2482.263 kb on - strand at 2482.282 kb on + strand at 2482.321 kb on + strand at 2482.388 kb on + strand at 2482.388 kb on + strand at 2482.388 kb on + strand at 2482.388 kb on + strand at 2482.389 kb on - strand at 2482.389 kb on - strand at 2482.389 kb on - strand at 2482.389 kb on - strand at 2482.389 kb on - strand at 2482.389 kb on - strand at 2482.527 kb on - strand at 2482.527 kb on - strand at 2482.542 kb on + strand at 2482.542 kb on + strand at 2482.543 kb on - strand at 2482.543 kb on - strand at 2482.543 kb on - strand at 2482.543 kb on - strand at 2482.543 kb on - strand at 2482.543 kb on - strand at 2482.574 kb on + strand at 2482.574 kb on + strand at 2482.604 kb on + strand at 2482.604 kb on + strand at 2482.604 kb on + strand at 2482.604 kb on + strand at 2482.605 kb on - strand at 2482.605 kb on - strand at 2482.605 kb on - strand at 2482.605 kb on - strand at 2482.605 kb on - strand at 2482.605 kb on - strand at 2482.605 kb on - strand at 2482.605 kb on - strand at 2482.605 kb on - strand at 2482.605 kb on - strand at 2482.605 kb on - strand at 2482.605 kb on - strand at 2482.605 kb on - strand at 2482.605 kb on - strand at 2482.605 kb on - strand at 2482.605 kb on - strand at 2482.605 kb on - strand at 2482.605 kb on - strand at 2482.605 kb on - strand at 2482.605 kb on - strand at 2482.605 kb on - strand at 2482.605 kb on - strand at 2482.673 kb on - strand, within Pf1N1B4_2168 at 2482.673 kb on - strand, within Pf1N1B4_2168 at 2482.673 kb on - strand, within Pf1N1B4_2168 at 2482.673 kb on - strand, within Pf1N1B4_2168 at 2482.673 kb on - strand, within Pf1N1B4_2168 at 2482.729 kb on + strand, within Pf1N1B4_2168 at 2482.736 kb on + strand, within Pf1N1B4_2168 at 2482.737 kb on - strand, within Pf1N1B4_2168 at 2482.737 kb on - strand, within Pf1N1B4_2168 at 2482.737 kb on - strand, within Pf1N1B4_2168 at 2482.737 kb on - strand, within Pf1N1B4_2168 at 2482.737 kb on - strand, within Pf1N1B4_2168 at 2482.737 kb on - strand, within Pf1N1B4_2168 at 2482.737 kb on - strand, within Pf1N1B4_2168 at 2482.737 kb on - strand, within Pf1N1B4_2168 at 2482.737 kb on - strand, within Pf1N1B4_2168 at 2482.737 kb on - strand, within Pf1N1B4_2168 at 2482.737 kb on - strand, within Pf1N1B4_2168 at 2482.737 kb on - strand, within Pf1N1B4_2168 at 2482.737 kb on - strand, within Pf1N1B4_2168 at 2482.781 kb on - strand at 2482.781 kb on - strand, within Pf1N1B4_2168 at 2482.837 kb on - strand, within Pf1N1B4_2168 at 2482.845 kb on - strand, within Pf1N1B4_2168 at 2482.845 kb on - strand, within Pf1N1B4_2168 at 2482.891 kb on + strand, within Pf1N1B4_2168 at 2482.891 kb on + strand, within Pf1N1B4_2168 at 2482.891 kb on + strand at 2482.892 kb on - strand at 2482.892 kb on - strand, within Pf1N1B4_2168 at 2482.892 kb on - strand, within Pf1N1B4_2168 at 2482.892 kb on - strand, within Pf1N1B4_2168 at 2482.892 kb on - strand, within Pf1N1B4_2168 at 2482.892 kb on - strand, within Pf1N1B4_2168 at 2482.892 kb on - strand, within Pf1N1B4_2168 at 2482.892 kb on - strand, within Pf1N1B4_2168 at 2482.959 kb on + strand, within Pf1N1B4_2168 at 2482.960 kb on - strand, within Pf1N1B4_2168 at 2482.960 kb on - strand, within Pf1N1B4_2168 at 2482.960 kb on - strand, within Pf1N1B4_2168 at 2482.960 kb on - strand, within Pf1N1B4_2168 at 2482.960 kb on - strand, within Pf1N1B4_2168 at 2482.995 kb on - strand at 2483.098 kb on - strand at 2483.099 kb on + strand at 2483.099 kb on + strand at 2483.099 kb on + strand at 2483.099 kb on + strand at 2483.099 kb on + strand at 2483.100 kb on - strand at 2483.100 kb on - strand at 2483.100 kb on - strand at 2483.100 kb on - strand at 2483.100 kb on - strand at 2483.100 kb on - strand at 2483.100 kb on - strand at 2483.100 kb on - strand at 2483.100 kb on - strand at 2483.100 kb on - strand at 2483.100 kb on - strand at 2483.100 kb on - strand at 2483.103 kb on + strand at 2483.103 kb on + strand at 2483.103 kb on + strand at 2483.103 kb on + strand at 2483.103 kb on + strand at 2483.103 kb on + strand at 2483.104 kb on - strand at 2483.104 kb on - strand at 2483.104 kb on - strand at 2483.104 kb on - strand at 2483.104 kb on - strand at 2483.104 kb on - strand at 2483.104 kb on - strand at 2483.104 kb on - strand at 2483.104 kb on - strand at 2483.104 kb on - strand at 2483.104 kb on - strand at 2483.129 kb on + strand at 2483.129 kb on + strand at 2483.129 kb on + strand at 2483.129 kb on + strand at 2483.129 kb on + strand at 2483.129 kb on + strand at 2483.129 kb on + strand at 2483.129 kb on + strand at 2483.129 kb on + strand at 2483.129 kb on + strand at 2483.129 kb on + strand at 2483.129 kb on + strand at 2483.130 kb on - strand at 2483.130 kb on - strand at 2483.130 kb on - strand at 2483.130 kb on - strand at 2483.130 kb on - strand at 2483.130 kb on - strand at 2483.130 kb on - strand at 2483.130 kb on - strand at 2483.130 kb on - strand at 2483.172 kb on + strand, within Pf1N1B4_2169 at 2483.172 kb on + strand, within Pf1N1B4_2169 at 2483.172 kb on + strand, within Pf1N1B4_2169 at 2483.172 kb on + strand, within Pf1N1B4_2169 at 2483.172 kb on + strand, within Pf1N1B4_2169 at 2483.173 kb on - strand, within Pf1N1B4_2169 at 2483.173 kb on - strand, within Pf1N1B4_2169 at 2483.173 kb on - strand, within Pf1N1B4_2169 at 2483.173 kb on - strand, within Pf1N1B4_2169
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source nitrite 0.5mM remove 2,480,294 + +2.1 2,480,294 + -0.0 2,480,294 + -0.6 2,480,294 + +1.4 2,480,294 + -0.6 2,480,294 + +0.6 2,480,294 + +1.4 2,480,295 - +0.1 2,480,295 - -2.6 2,480,308 + +0.8 2,480,308 + +1.2 2,480,308 + +0.3 2,480,308 + +0.4 2,480,308 + +0.5 2,480,308 + -0.2 2,480,308 + +1.4 2,480,308 + +0.0 2,480,308 + +1.2 2,480,308 + +0.4 2,480,308 + +0.4 2,480,309 - -2.0 2,480,309 - +0.1 2,480,310 + +0.4 2,480,310 + +0.3 2,480,311 - -0.7 2,480,453 - -0.1 2,480,560 - -0.2 2,480,620 + -0.5 2,480,620 + +0.4 2,480,620 + +0.6 2,481,015 + -0.0 2,481,016 - -0.5 2,481,052 + -0.9 2,481,052 + -0.0 2,481,052 + -0.6 2,481,052 + +0.3 2,481,053 - -0.8 2,481,078 + -1.1 2,481,228 - Pf1N1B4_2167 0.13 +0.2 2,481,317 + Pf1N1B4_2167 0.20 -0.3 2,481,317 + Pf1N1B4_2167 0.20 -2.7 2,481,317 + Pf1N1B4_2167 0.20 -2.9 2,481,317 + Pf1N1B4_2167 0.20 -1.3 2,481,317 + Pf1N1B4_2167 0.20 -1.5 2,481,318 - Pf1N1B4_2167 0.20 -0.7 2,481,318 - Pf1N1B4_2167 0.20 -0.6 2,481,323 + Pf1N1B4_2167 0.20 -2.3 2,481,587 + +1.4 2,481,587 + Pf1N1B4_2167 0.40 -1.3 2,481,587 + Pf1N1B4_2167 0.40 -1.5 2,481,587 + Pf1N1B4_2167 0.40 -0.3 2,481,588 - Pf1N1B4_2167 0.40 -2.8 2,481,588 - Pf1N1B4_2167 0.40 -1.7 2,481,588 - Pf1N1B4_2167 0.40 -1.4 2,481,773 + Pf1N1B4_2167 0.54 +1.1 2,481,774 - Pf1N1B4_2167 0.54 -3.1 2,481,870 + Pf1N1B4_2167 0.61 -3.0 2,481,870 + Pf1N1B4_2167 0.61 -1.9 2,481,870 + Pf1N1B4_2167 0.61 -3.7 2,481,871 - Pf1N1B4_2167 0.61 -1.2 2,481,871 - Pf1N1B4_2167 0.61 -2.3 2,482,262 + -1.6 2,482,262 + -1.6 2,482,262 + -3.0 2,482,262 + -2.0 2,482,262 + +0.1 2,482,263 - -2.0 2,482,263 - -0.4 2,482,263 - -2.8 2,482,282 + -2.2 2,482,321 + -1.0 2,482,388 + -1.1 2,482,388 + -1.2 2,482,388 + -0.6 2,482,388 + +1.2 2,482,389 - -0.6 2,482,389 - +0.5 2,482,389 - -0.6 2,482,389 - -1.1 2,482,389 - -1.9 2,482,389 - -1.9 2,482,527 - -0.6 2,482,527 - +0.3 2,482,542 + +0.6 2,482,542 + -1.0 2,482,543 - -1.3 2,482,543 - +0.9 2,482,543 - -0.6 2,482,543 - -1.2 2,482,543 - +0.2 2,482,543 - +1.0 2,482,574 + +0.5 2,482,574 + +0.7 2,482,604 + +1.9 2,482,604 + -0.5 2,482,604 + +1.4 2,482,604 + -0.6 2,482,605 - +1.0 2,482,605 - +0.7 2,482,605 - -0.4 2,482,605 - -1.5 2,482,605 - -0.1 2,482,605 - -0.8 2,482,605 - +0.8 2,482,605 - +1.7 2,482,605 - +1.0 2,482,605 - +0.4 2,482,605 - -0.4 2,482,605 - -0.5 2,482,605 - -0.7 2,482,605 - +1.4 2,482,605 - -0.1 2,482,605 - -2.7 2,482,605 - +0.4 2,482,605 - -1.9 2,482,605 - +0.5 2,482,605 - -0.3 2,482,605 - -0.6 2,482,605 - -1.5 2,482,673 - Pf1N1B4_2168 0.16 +1.0 2,482,673 - Pf1N1B4_2168 0.16 -1.7 2,482,673 - Pf1N1B4_2168 0.16 -0.4 2,482,673 - Pf1N1B4_2168 0.16 -1.8 2,482,673 - Pf1N1B4_2168 0.16 -0.0 2,482,729 + Pf1N1B4_2168 0.29 +0.0 2,482,736 + Pf1N1B4_2168 0.31 -1.9 2,482,737 - Pf1N1B4_2168 0.31 +0.2 2,482,737 - Pf1N1B4_2168 0.31 +0.2 2,482,737 - Pf1N1B4_2168 0.31 -2.2 2,482,737 - Pf1N1B4_2168 0.31 -2.2 2,482,737 - Pf1N1B4_2168 0.31 +0.9 2,482,737 - Pf1N1B4_2168 0.31 -2.3 2,482,737 - Pf1N1B4_2168 0.31 -0.9 2,482,737 - Pf1N1B4_2168 0.31 +0.2 2,482,737 - Pf1N1B4_2168 0.31 -1.5 2,482,737 - Pf1N1B4_2168 0.31 +0.3 2,482,737 - Pf1N1B4_2168 0.31 -0.5 2,482,737 - Pf1N1B4_2168 0.31 +0.6 2,482,737 - Pf1N1B4_2168 0.31 +0.3 2,482,781 - +0.8 2,482,781 - Pf1N1B4_2168 0.41 -0.9 2,482,837 - Pf1N1B4_2168 0.54 -0.2 2,482,845 - Pf1N1B4_2168 0.56 -1.5 2,482,845 - Pf1N1B4_2168 0.56 -1.1 2,482,891 + Pf1N1B4_2168 0.67 +0.8 2,482,891 + Pf1N1B4_2168 0.67 -0.2 2,482,891 + -2.2 2,482,892 - -2.1 2,482,892 - Pf1N1B4_2168 0.67 +1.2 2,482,892 - Pf1N1B4_2168 0.67 -0.6 2,482,892 - Pf1N1B4_2168 0.67 +1.0 2,482,892 - Pf1N1B4_2168 0.67 -1.2 2,482,892 - Pf1N1B4_2168 0.67 -1.5 2,482,892 - Pf1N1B4_2168 0.67 -0.4 2,482,892 - Pf1N1B4_2168 0.67 +0.3 2,482,959 + Pf1N1B4_2168 0.83 +0.4 2,482,960 - Pf1N1B4_2168 0.83 -3.4 2,482,960 - Pf1N1B4_2168 0.83 +0.4 2,482,960 - Pf1N1B4_2168 0.83 +0.5 2,482,960 - Pf1N1B4_2168 0.83 -0.3 2,482,960 - Pf1N1B4_2168 0.83 +0.1 2,482,995 - +2.0 2,483,098 - +0.8 2,483,099 + -0.5 2,483,099 + +0.5 2,483,099 + +0.4 2,483,099 + -0.2 2,483,099 + +0.7 2,483,100 - -0.7 2,483,100 - +0.5 2,483,100 - -2.0 2,483,100 - -0.1 2,483,100 - +1.4 2,483,100 - +0.2 2,483,100 - +0.8 2,483,100 - -0.3 2,483,100 - +1.6 2,483,100 - -0.2 2,483,100 - -1.5 2,483,100 - -0.0 2,483,103 + -2.7 2,483,103 + +1.0 2,483,103 + +0.6 2,483,103 + -0.3 2,483,103 + -0.8 2,483,103 + -0.6 2,483,104 - -0.6 2,483,104 - -0.3 2,483,104 - -0.8 2,483,104 - -1.5 2,483,104 - +1.1 2,483,104 - -0.5 2,483,104 - -1.1 2,483,104 - -1.7 2,483,104 - -1.0 2,483,104 - -2.0 2,483,104 - +0.2 2,483,129 + -0.6 2,483,129 + -1.9 2,483,129 + -0.2 2,483,129 + -0.5 2,483,129 + -1.4 2,483,129 + +1.1 2,483,129 + +2.0 2,483,129 + -1.7 2,483,129 + -0.2 2,483,129 + +0.4 2,483,129 + +0.7 2,483,129 + -0.2 2,483,130 - -1.0 2,483,130 - -1.7 2,483,130 - -0.3 2,483,130 - +1.1 2,483,130 - -0.7 2,483,130 - -1.0 2,483,130 - -0.7 2,483,130 - -1.3 2,483,130 - +0.3 2,483,172 + Pf1N1B4_2169 0.11 +1.2 2,483,172 + Pf1N1B4_2169 0.11 +0.3 2,483,172 + Pf1N1B4_2169 0.11 -1.9 2,483,172 + Pf1N1B4_2169 0.11 +0.3 2,483,172 + Pf1N1B4_2169 0.11 -1.1 2,483,173 - Pf1N1B4_2169 0.12 -0.8 2,483,173 - Pf1N1B4_2169 0.12 +1.2 2,483,173 - Pf1N1B4_2169 0.12 +1.6 2,483,173 - Pf1N1B4_2169 0.12 -0.6
Or see this region's nucleotide sequence