Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_138

Experiment: nitrogen source nitrite 0.5mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_137 and Pf1N1B4_138 are separated by 269 nucleotidesPf1N1B4_138 and Pf1N1B4_139 overlap by 4 nucleotides Pf1N1B4_137 - Pyoverdine biosynthesis related protein PvdP, at 152,644 to 154,269 _137 Pf1N1B4_138 - Putative dipeptidase, pyoverdin biosynthesis PvdM, at 154,539 to 155,915 _138 Pf1N1B4_139 - Pyoverdin biosynthesis protein PvdN, putative aminotransferase, class V, at 155,912 to 157,198 _139 Position (kb) 154 155 156Strain fitness (log2 ratio) -2 -1 0 1at 153.849 kb on - strand, within Pf1N1B4_137at 153.849 kb on - strand, within Pf1N1B4_137at 153.849 kb on - strand, within Pf1N1B4_137at 153.894 kb on - strand, within Pf1N1B4_137at 153.983 kb on + strand, within Pf1N1B4_137at 154.200 kb on - strandat 154.311 kb on + strandat 154.312 kb on - strandat 154.312 kb on - strandat 154.312 kb on - strandat 154.322 kb on + strandat 154.323 kb on - strandat 154.338 kb on - strandat 154.351 kb on + strandat 154.352 kb on - strandat 154.428 kb on + strandat 154.575 kb on + strandat 154.576 kb on - strandat 154.576 kb on - strandat 155.611 kb on - strandat 155.670 kb on + strand, within Pf1N1B4_138at 155.671 kb on - strand, within Pf1N1B4_138at 156.071 kb on + strand, within Pf1N1B4_139at 156.072 kb on - strand, within Pf1N1B4_139at 156.072 kb on - strand, within Pf1N1B4_139at 156.360 kb on - strand, within Pf1N1B4_139at 156.360 kb on - strand, within Pf1N1B4_139at 156.360 kb on - strand, within Pf1N1B4_139at 156.617 kb on + strand, within Pf1N1B4_139at 156.618 kb on - strand, within Pf1N1B4_139at 156.618 kb on - strand, within Pf1N1B4_139

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Per-strain Table

Position Strand Gene LocusTag Fraction nitrogen source nitrite 0.5mM
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153,849 - Pf1N1B4_137 0.74 +1.4
153,849 - Pf1N1B4_137 0.74 -1.2
153,849 - Pf1N1B4_137 0.74 +0.5
153,894 - Pf1N1B4_137 0.77 -2.4
153,983 + Pf1N1B4_137 0.82 +1.6
154,200 - -2.5
154,311 + -0.1
154,312 - -0.1
154,312 - +0.6
154,312 - -1.6
154,322 + +0.4
154,323 - -2.4
154,338 - +0.8
154,351 + -0.3
154,352 - -1.4
154,428 + -1.4
154,575 + -1.5
154,576 - +0.7
154,576 - -1.9
155,611 - -0.3
155,670 + Pf1N1B4_138 0.82 -0.9
155,671 - Pf1N1B4_138 0.82 -2.4
156,071 + Pf1N1B4_139 0.12 -2.4
156,072 - Pf1N1B4_139 0.12 +1.1
156,072 - Pf1N1B4_139 0.12 -2.4
156,360 - Pf1N1B4_139 0.35 +0.4
156,360 - Pf1N1B4_139 0.35 +0.2
156,360 - Pf1N1B4_139 0.35 -2.0
156,617 + Pf1N1B4_139 0.55 -1.6
156,618 - Pf1N1B4_139 0.55 +0.3
156,618 - Pf1N1B4_139 0.55 -2.2

Or see this region's nucleotide sequence