Experiment: nitrogen source nitrite 0.5mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1022 and Pf1N1B4_1023 are separated by 111 nucleotides Pf1N1B4_1023 and Pf1N1B4_1024 are separated by 12 nucleotides Pf1N1B4_1024 and Pf1N1B4_1025 are separated by 2 nucleotides
Pf1N1B4_1022 - Protein sirB1, at 1,157,813 to 1,158,616
_1022
Pf1N1B4_1023 - Maleylacetoacetate isomerase (EC 5.2.1.2) @ Glutathione S-transferase, zeta (EC 2.5.1.18), at 1,158,728 to 1,159,363
_1023
Pf1N1B4_1024 - Fumarylacetoacetase (EC 3.7.1.2), at 1,159,376 to 1,160,680
_1024
Pf1N1B4_1025 - Homogentisate 1,2-dioxygenase (EC 1.13.11.5), at 1,160,683 to 1,161,987
_1025
Position (kb)
1159
1160
1161 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1158.380 kb on + strand, within Pf1N1B4_1022 at 1158.381 kb on - strand, within Pf1N1B4_1022 at 1158.381 kb on - strand, within Pf1N1B4_1022 at 1158.381 kb on - strand, within Pf1N1B4_1022 at 1158.398 kb on + strand, within Pf1N1B4_1022 at 1158.398 kb on + strand, within Pf1N1B4_1022 at 1158.398 kb on + strand, within Pf1N1B4_1022 at 1158.398 kb on + strand, within Pf1N1B4_1022 at 1158.398 kb on + strand, within Pf1N1B4_1022 at 1158.398 kb on + strand, within Pf1N1B4_1022 at 1158.398 kb on + strand, within Pf1N1B4_1022 at 1158.398 kb on + strand, within Pf1N1B4_1022 at 1158.399 kb on - strand, within Pf1N1B4_1022 at 1158.399 kb on - strand, within Pf1N1B4_1022 at 1158.399 kb on - strand, within Pf1N1B4_1022 at 1158.462 kb on - strand, within Pf1N1B4_1022 at 1158.462 kb on - strand, within Pf1N1B4_1022 at 1158.591 kb on + strand at 1158.592 kb on - strand at 1158.598 kb on + strand at 1158.599 kb on - strand at 1158.711 kb on + strand at 1158.711 kb on + strand at 1158.711 kb on + strand at 1158.711 kb on + strand at 1158.711 kb on + strand at 1158.711 kb on + strand at 1158.712 kb on - strand at 1158.717 kb on + strand at 1158.717 kb on + strand at 1158.717 kb on + strand at 1158.717 kb on + strand at 1158.717 kb on + strand at 1158.717 kb on + strand at 1158.717 kb on + strand at 1158.718 kb on - strand at 1158.718 kb on - strand at 1158.718 kb on - strand at 1158.718 kb on - strand at 1158.718 kb on - strand at 1158.718 kb on - strand at 1158.718 kb on - strand at 1158.832 kb on + strand, within Pf1N1B4_1023 at 1158.832 kb on + strand, within Pf1N1B4_1023 at 1158.833 kb on - strand, within Pf1N1B4_1023 at 1158.833 kb on - strand, within Pf1N1B4_1023 at 1158.833 kb on - strand, within Pf1N1B4_1023 at 1158.833 kb on - strand, within Pf1N1B4_1023 at 1158.833 kb on - strand, within Pf1N1B4_1023 at 1158.852 kb on + strand, within Pf1N1B4_1023 at 1158.852 kb on + strand, within Pf1N1B4_1023 at 1158.852 kb on + strand, within Pf1N1B4_1023 at 1158.853 kb on - strand, within Pf1N1B4_1023 at 1158.853 kb on - strand, within Pf1N1B4_1023 at 1158.853 kb on - strand, within Pf1N1B4_1023 at 1158.853 kb on - strand, within Pf1N1B4_1023 at 1158.870 kb on + strand, within Pf1N1B4_1023 at 1158.870 kb on + strand, within Pf1N1B4_1023 at 1158.870 kb on + strand, within Pf1N1B4_1023 at 1158.870 kb on + strand, within Pf1N1B4_1023 at 1158.871 kb on - strand, within Pf1N1B4_1023 at 1158.871 kb on - strand, within Pf1N1B4_1023 at 1158.871 kb on - strand, within Pf1N1B4_1023 at 1159.140 kb on + strand, within Pf1N1B4_1023 at 1159.141 kb on - strand, within Pf1N1B4_1023 at 1159.141 kb on - strand, within Pf1N1B4_1023 at 1159.227 kb on + strand, within Pf1N1B4_1023 at 1159.227 kb on + strand, within Pf1N1B4_1023 at 1159.228 kb on - strand, within Pf1N1B4_1023 at 1159.228 kb on - strand, within Pf1N1B4_1023 at 1159.287 kb on + strand at 1159.316 kb on - strand at 1159.316 kb on - strand at 1159.345 kb on - strand at 1159.345 kb on - strand at 1159.347 kb on + strand at 1159.347 kb on + strand at 1159.347 kb on + strand at 1159.347 kb on + strand at 1159.347 kb on + strand at 1159.347 kb on + strand at 1159.347 kb on + strand at 1159.347 kb on + strand at 1159.347 kb on + strand at 1159.347 kb on + strand at 1159.347 kb on + strand at 1159.348 kb on - strand at 1159.348 kb on - strand at 1159.348 kb on - strand at 1159.348 kb on - strand at 1159.348 kb on - strand at 1159.351 kb on + strand at 1159.351 kb on + strand at 1159.352 kb on - strand at 1159.352 kb on - strand at 1159.353 kb on + strand at 1159.353 kb on + strand at 1159.353 kb on + strand at 1159.353 kb on + strand at 1159.353 kb on + strand at 1159.354 kb on - strand at 1159.354 kb on - strand at 1159.354 kb on - strand at 1159.354 kb on - strand at 1159.354 kb on - strand at 1159.354 kb on - strand at 1159.354 kb on - strand at 1159.354 kb on - strand at 1159.378 kb on - strand at 1159.487 kb on - strand at 1159.662 kb on + strand, within Pf1N1B4_1024 at 1159.662 kb on + strand, within Pf1N1B4_1024 at 1159.662 kb on + strand, within Pf1N1B4_1024 at 1159.662 kb on + strand, within Pf1N1B4_1024 at 1159.663 kb on - strand, within Pf1N1B4_1024 at 1159.663 kb on - strand, within Pf1N1B4_1024 at 1160.199 kb on + strand, within Pf1N1B4_1024 at 1160.199 kb on + strand, within Pf1N1B4_1024 at 1160.199 kb on + strand, within Pf1N1B4_1024 at 1160.199 kb on + strand, within Pf1N1B4_1024 at 1160.200 kb on - strand, within Pf1N1B4_1024 at 1160.200 kb on - strand, within Pf1N1B4_1024 at 1160.200 kb on - strand, within Pf1N1B4_1024 at 1160.205 kb on + strand, within Pf1N1B4_1024 at 1160.205 kb on + strand, within Pf1N1B4_1024 at 1160.205 kb on + strand, within Pf1N1B4_1024 at 1160.205 kb on + strand, within Pf1N1B4_1024 at 1160.206 kb on - strand, within Pf1N1B4_1024 at 1160.274 kb on + strand, within Pf1N1B4_1024 at 1160.274 kb on + strand, within Pf1N1B4_1024 at 1160.274 kb on + strand, within Pf1N1B4_1024 at 1160.274 kb on + strand, within Pf1N1B4_1024 at 1160.542 kb on + strand, within Pf1N1B4_1024 at 1160.543 kb on - strand, within Pf1N1B4_1024 at 1160.657 kb on - strand at 1160.684 kb on + strand at 1160.735 kb on + strand at 1160.736 kb on - strand at 1160.736 kb on - strand at 1160.830 kb on - strand, within Pf1N1B4_1025 at 1160.948 kb on + strand, within Pf1N1B4_1025 at 1160.949 kb on - strand, within Pf1N1B4_1025 at 1160.949 kb on - strand, within Pf1N1B4_1025 at 1161.147 kb on + strand, within Pf1N1B4_1025 at 1161.167 kb on + strand, within Pf1N1B4_1025 at 1161.167 kb on + strand, within Pf1N1B4_1025 at 1161.167 kb on + strand, within Pf1N1B4_1025 at 1161.168 kb on - strand, within Pf1N1B4_1025 at 1161.168 kb on - strand, within Pf1N1B4_1025 at 1161.168 kb on - strand, within Pf1N1B4_1025 at 1161.238 kb on + strand, within Pf1N1B4_1025 at 1161.238 kb on + strand, within Pf1N1B4_1025 at 1161.239 kb on - strand, within Pf1N1B4_1025 at 1161.259 kb on + strand, within Pf1N1B4_1025 at 1161.259 kb on + strand, within Pf1N1B4_1025 at 1161.290 kb on + strand, within Pf1N1B4_1025 at 1161.291 kb on - strand, within Pf1N1B4_1025 at 1161.336 kb on + strand, within Pf1N1B4_1025 at 1161.395 kb on + strand, within Pf1N1B4_1025 at 1161.395 kb on + strand, within Pf1N1B4_1025 at 1161.395 kb on + strand, within Pf1N1B4_1025 at 1161.585 kb on - strand, within Pf1N1B4_1025 at 1161.588 kb on + strand, within Pf1N1B4_1025 at 1161.588 kb on + strand, within Pf1N1B4_1025 at 1161.588 kb on + strand, within Pf1N1B4_1025 at 1161.588 kb on + strand, within Pf1N1B4_1025 at 1161.588 kb on + strand, within Pf1N1B4_1025 at 1161.589 kb on - strand, within Pf1N1B4_1025 at 1161.589 kb on - strand, within Pf1N1B4_1025 at 1161.590 kb on + strand, within Pf1N1B4_1025 at 1161.590 kb on + strand, within Pf1N1B4_1025 at 1161.590 kb on + strand, within Pf1N1B4_1025 at 1161.590 kb on + strand, within Pf1N1B4_1025 at 1161.590 kb on + strand, within Pf1N1B4_1025 at 1161.590 kb on + strand, within Pf1N1B4_1025 at 1161.590 kb on + strand, within Pf1N1B4_1025 at 1161.590 kb on + strand, within Pf1N1B4_1025 at 1161.590 kb on + strand, within Pf1N1B4_1025 at 1161.590 kb on + strand, within Pf1N1B4_1025 at 1161.591 kb on - strand, within Pf1N1B4_1025 at 1161.591 kb on - strand, within Pf1N1B4_1025 at 1161.591 kb on - strand, within Pf1N1B4_1025 at 1161.591 kb on - strand at 1161.591 kb on - strand, within Pf1N1B4_1025 at 1161.594 kb on + strand, within Pf1N1B4_1025 at 1161.594 kb on + strand, within Pf1N1B4_1025 at 1161.594 kb on + strand, within Pf1N1B4_1025 at 1161.594 kb on + strand, within Pf1N1B4_1025 at 1161.595 kb on - strand, within Pf1N1B4_1025 at 1161.595 kb on - strand, within Pf1N1B4_1025
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source nitrite 0.5mM remove 1,158,380 + Pf1N1B4_1022 0.71 -0.6 1,158,381 - Pf1N1B4_1022 0.71 +0.1 1,158,381 - Pf1N1B4_1022 0.71 -1.2 1,158,381 - Pf1N1B4_1022 0.71 -0.4 1,158,398 + Pf1N1B4_1022 0.73 -0.4 1,158,398 + Pf1N1B4_1022 0.73 +0.6 1,158,398 + Pf1N1B4_1022 0.73 +2.1 1,158,398 + Pf1N1B4_1022 0.73 +0.1 1,158,398 + Pf1N1B4_1022 0.73 -0.1 1,158,398 + Pf1N1B4_1022 0.73 -0.5 1,158,398 + Pf1N1B4_1022 0.73 +0.2 1,158,398 + Pf1N1B4_1022 0.73 +0.7 1,158,399 - Pf1N1B4_1022 0.73 -0.3 1,158,399 - Pf1N1B4_1022 0.73 +2.3 1,158,399 - Pf1N1B4_1022 0.73 -0.7 1,158,462 - Pf1N1B4_1022 0.81 +0.4 1,158,462 - Pf1N1B4_1022 0.81 -0.1 1,158,591 + -1.4 1,158,592 - +0.3 1,158,598 + +0.5 1,158,599 - -1.6 1,158,711 + -1.9 1,158,711 + -0.7 1,158,711 + -0.8 1,158,711 + -0.3 1,158,711 + +1.4 1,158,711 + +0.2 1,158,712 - +2.0 1,158,717 + -0.8 1,158,717 + +0.6 1,158,717 + -1.4 1,158,717 + +0.7 1,158,717 + +2.2 1,158,717 + -0.4 1,158,717 + +1.3 1,158,718 - +0.5 1,158,718 - -1.3 1,158,718 - -0.8 1,158,718 - +0.8 1,158,718 - -0.7 1,158,718 - -0.8 1,158,718 - +1.4 1,158,832 + Pf1N1B4_1023 0.16 -0.2 1,158,832 + Pf1N1B4_1023 0.16 -0.0 1,158,833 - Pf1N1B4_1023 0.17 -2.7 1,158,833 - Pf1N1B4_1023 0.17 -0.4 1,158,833 - Pf1N1B4_1023 0.17 +0.1 1,158,833 - Pf1N1B4_1023 0.17 -0.5 1,158,833 - Pf1N1B4_1023 0.17 -1.0 1,158,852 + Pf1N1B4_1023 0.19 -3.2 1,158,852 + Pf1N1B4_1023 0.19 -1.2 1,158,852 + Pf1N1B4_1023 0.19 -0.1 1,158,853 - Pf1N1B4_1023 0.20 -1.7 1,158,853 - Pf1N1B4_1023 0.20 +0.7 1,158,853 - Pf1N1B4_1023 0.20 -4.7 1,158,853 - Pf1N1B4_1023 0.20 +0.6 1,158,870 + Pf1N1B4_1023 0.22 -0.2 1,158,870 + Pf1N1B4_1023 0.22 -2.6 1,158,870 + Pf1N1B4_1023 0.22 -2.0 1,158,870 + Pf1N1B4_1023 0.22 +1.3 1,158,871 - Pf1N1B4_1023 0.22 -1.0 1,158,871 - Pf1N1B4_1023 0.22 +0.7 1,158,871 - Pf1N1B4_1023 0.22 -0.2 1,159,140 + Pf1N1B4_1023 0.65 -2.7 1,159,141 - Pf1N1B4_1023 0.65 +1.0 1,159,141 - Pf1N1B4_1023 0.65 -0.1 1,159,227 + Pf1N1B4_1023 0.78 -0.5 1,159,227 + Pf1N1B4_1023 0.78 +0.4 1,159,228 - Pf1N1B4_1023 0.79 -0.4 1,159,228 - Pf1N1B4_1023 0.79 -0.2 1,159,287 + -1.5 1,159,316 - -1.7 1,159,316 - -0.0 1,159,345 - -1.4 1,159,345 - +1.0 1,159,347 + +2.2 1,159,347 + +0.3 1,159,347 + +0.6 1,159,347 + -0.7 1,159,347 + +1.5 1,159,347 + +2.4 1,159,347 + +1.1 1,159,347 + -1.4 1,159,347 + +0.2 1,159,347 + +0.6 1,159,347 + -1.5 1,159,348 - +2.0 1,159,348 - -0.2 1,159,348 - -1.8 1,159,348 - +1.0 1,159,348 - -1.4 1,159,351 + -1.4 1,159,351 + -0.2 1,159,352 - +0.1 1,159,352 - -0.8 1,159,353 + +0.1 1,159,353 + +0.4 1,159,353 + -0.9 1,159,353 + +1.1 1,159,353 + +0.1 1,159,354 - -0.4 1,159,354 - -1.7 1,159,354 - +0.1 1,159,354 - +0.4 1,159,354 - -1.0 1,159,354 - -0.4 1,159,354 - +2.5 1,159,354 - +0.4 1,159,378 - -1.0 1,159,487 - -3.1 1,159,662 + Pf1N1B4_1024 0.22 +0.5 1,159,662 + Pf1N1B4_1024 0.22 -2.2 1,159,662 + Pf1N1B4_1024 0.22 -3.1 1,159,662 + Pf1N1B4_1024 0.22 +0.3 1,159,663 - Pf1N1B4_1024 0.22 -1.0 1,159,663 - Pf1N1B4_1024 0.22 -1.4 1,160,199 + Pf1N1B4_1024 0.63 -3.3 1,160,199 + Pf1N1B4_1024 0.63 +2.7 1,160,199 + Pf1N1B4_1024 0.63 +0.4 1,160,199 + Pf1N1B4_1024 0.63 -1.7 1,160,200 - Pf1N1B4_1024 0.63 -3.0 1,160,200 - Pf1N1B4_1024 0.63 -1.6 1,160,200 - Pf1N1B4_1024 0.63 -2.4 1,160,205 + Pf1N1B4_1024 0.64 +0.4 1,160,205 + Pf1N1B4_1024 0.64 -0.7 1,160,205 + Pf1N1B4_1024 0.64 -3.3 1,160,205 + Pf1N1B4_1024 0.64 -0.2 1,160,206 - Pf1N1B4_1024 0.64 -2.3 1,160,274 + Pf1N1B4_1024 0.69 -0.9 1,160,274 + Pf1N1B4_1024 0.69 -1.1 1,160,274 + Pf1N1B4_1024 0.69 -4.0 1,160,274 + Pf1N1B4_1024 0.69 -0.6 1,160,542 + Pf1N1B4_1024 0.89 -1.5 1,160,543 - Pf1N1B4_1024 0.89 -0.3 1,160,657 - -1.2 1,160,684 + -1.4 1,160,735 + -0.3 1,160,736 - +0.6 1,160,736 - +0.6 1,160,830 - Pf1N1B4_1025 0.11 -0.5 1,160,948 + Pf1N1B4_1025 0.20 +1.1 1,160,949 - Pf1N1B4_1025 0.20 +0.8 1,160,949 - Pf1N1B4_1025 0.20 +0.1 1,161,147 + Pf1N1B4_1025 0.36 +0.2 1,161,167 + Pf1N1B4_1025 0.37 +0.2 1,161,167 + Pf1N1B4_1025 0.37 -0.4 1,161,167 + Pf1N1B4_1025 0.37 -0.1 1,161,168 - Pf1N1B4_1025 0.37 -0.2 1,161,168 - Pf1N1B4_1025 0.37 +0.6 1,161,168 - Pf1N1B4_1025 0.37 +1.8 1,161,238 + Pf1N1B4_1025 0.43 -0.6 1,161,238 + Pf1N1B4_1025 0.43 +0.4 1,161,239 - Pf1N1B4_1025 0.43 +0.1 1,161,259 + Pf1N1B4_1025 0.44 +0.6 1,161,259 + Pf1N1B4_1025 0.44 -0.6 1,161,290 + Pf1N1B4_1025 0.47 -1.6 1,161,291 - Pf1N1B4_1025 0.47 +1.2 1,161,336 + Pf1N1B4_1025 0.50 -0.2 1,161,395 + Pf1N1B4_1025 0.55 +0.9 1,161,395 + Pf1N1B4_1025 0.55 +0.7 1,161,395 + Pf1N1B4_1025 0.55 -2.7 1,161,585 - Pf1N1B4_1025 0.69 -0.2 1,161,588 + Pf1N1B4_1025 0.69 +0.3 1,161,588 + Pf1N1B4_1025 0.69 -0.1 1,161,588 + Pf1N1B4_1025 0.69 -0.2 1,161,588 + Pf1N1B4_1025 0.69 -0.2 1,161,588 + Pf1N1B4_1025 0.69 +0.0 1,161,589 - Pf1N1B4_1025 0.69 +0.7 1,161,589 - Pf1N1B4_1025 0.69 +1.2 1,161,590 + Pf1N1B4_1025 0.70 -0.7 1,161,590 + Pf1N1B4_1025 0.70 -0.4 1,161,590 + Pf1N1B4_1025 0.70 +0.7 1,161,590 + Pf1N1B4_1025 0.70 -0.0 1,161,590 + Pf1N1B4_1025 0.70 -0.5 1,161,590 + Pf1N1B4_1025 0.70 +0.3 1,161,590 + Pf1N1B4_1025 0.70 -1.3 1,161,590 + Pf1N1B4_1025 0.70 +1.1 1,161,590 + Pf1N1B4_1025 0.70 +0.4 1,161,590 + Pf1N1B4_1025 0.70 +0.1 1,161,591 - Pf1N1B4_1025 0.70 +1.3 1,161,591 - Pf1N1B4_1025 0.70 +0.9 1,161,591 - Pf1N1B4_1025 0.70 -0.2 1,161,591 - -1.2 1,161,591 - Pf1N1B4_1025 0.70 +0.9 1,161,594 + Pf1N1B4_1025 0.70 -3.5 1,161,594 + Pf1N1B4_1025 0.70 -0.3 1,161,594 + Pf1N1B4_1025 0.70 +0.1 1,161,594 + Pf1N1B4_1025 0.70 -0.3 1,161,595 - Pf1N1B4_1025 0.70 -1.0 1,161,595 - Pf1N1B4_1025 0.70 -1.3
Or see this region's nucleotide sequence