Experiment: Phaeo_ML1_MIT diversity barseq
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c09980 and PGA1_c09990 are separated by 93 nucleotides PGA1_c09990 and PGA1_c10000 overlap by 1 nucleotides PGA1_c10000 and PGA1_c10010 are separated by 246 nucleotides
PGA1_c09980: PGA1_c09980 - kynureninase KynU, at 1,031,352 to 1,032,545
_c09980
PGA1_c09990: PGA1_c09990 - Uncharacterized protein conserved in bacteria, at 1,032,639 to 1,033,037
_c09990
PGA1_c10000: PGA1_c10000 - short chain dehydrogenase / reductase, at 1,033,037 to 1,033,789
_c10000
PGA1_c10010: PGA1_c10010 - putative protein SoxH, at 1,034,036 to 1,034,989
_c10010
Position (kb)
1033
1034 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 1032.040 kb on + strand, within PGA1_c09980 at 1032.061 kb on + strand, within PGA1_c09980 at 1032.076 kb on + strand, within PGA1_c09980 at 1032.095 kb on + strand, within PGA1_c09980 at 1032.113 kb on + strand, within PGA1_c09980 at 1032.123 kb on + strand, within PGA1_c09980 at 1032.149 kb on + strand, within PGA1_c09980 at 1032.163 kb on - strand, within PGA1_c09980 at 1032.180 kb on + strand, within PGA1_c09980 at 1032.180 kb on + strand, within PGA1_c09980 at 1032.188 kb on - strand, within PGA1_c09980 at 1032.279 kb on - strand, within PGA1_c09980 at 1032.325 kb on + strand, within PGA1_c09980 at 1032.333 kb on - strand, within PGA1_c09980 at 1032.387 kb on + strand, within PGA1_c09980 at 1032.387 kb on + strand, within PGA1_c09980 at 1032.390 kb on - strand, within PGA1_c09980 at 1032.390 kb on - strand, within PGA1_c09980 at 1032.405 kb on - strand, within PGA1_c09980 at 1032.487 kb on + strand at 1032.487 kb on + strand at 1032.543 kb on + strand at 1032.543 kb on + strand at 1032.545 kb on + strand at 1032.680 kb on + strand, within PGA1_c09990 at 1032.735 kb on + strand, within PGA1_c09990 at 1032.744 kb on - strand, within PGA1_c09990 at 1032.744 kb on - strand, within PGA1_c09990 at 1032.744 kb on - strand, within PGA1_c09990 at 1032.745 kb on + strand, within PGA1_c09990 at 1032.750 kb on + strand, within PGA1_c09990 at 1032.779 kb on + strand, within PGA1_c09990 at 1032.959 kb on + strand, within PGA1_c09990 at 1032.991 kb on - strand, within PGA1_c09990 at 1032.991 kb on - strand, within PGA1_c09990 at 1033.033 kb on - strand at 1033.040 kb on + strand at 1033.040 kb on + strand at 1033.089 kb on - strand at 1033.144 kb on - strand, within PGA1_c10000 at 1033.165 kb on + strand, within PGA1_c10000 at 1033.197 kb on + strand, within PGA1_c10000 at 1033.207 kb on + strand, within PGA1_c10000 at 1033.281 kb on + strand, within PGA1_c10000 at 1033.283 kb on - strand, within PGA1_c10000 at 1033.321 kb on - strand, within PGA1_c10000 at 1033.335 kb on - strand, within PGA1_c10000 at 1033.378 kb on - strand, within PGA1_c10000 at 1033.406 kb on + strand, within PGA1_c10000 at 1033.438 kb on + strand, within PGA1_c10000 at 1033.514 kb on - strand, within PGA1_c10000 at 1033.526 kb on - strand, within PGA1_c10000 at 1033.526 kb on - strand, within PGA1_c10000 at 1033.526 kb on - strand, within PGA1_c10000 at 1033.526 kb on - strand, within PGA1_c10000 at 1033.561 kb on - strand, within PGA1_c10000 at 1033.561 kb on - strand, within PGA1_c10000 at 1033.561 kb on - strand, within PGA1_c10000 at 1033.561 kb on - strand, within PGA1_c10000 at 1033.623 kb on + strand, within PGA1_c10000 at 1033.669 kb on + strand, within PGA1_c10000 at 1033.677 kb on - strand, within PGA1_c10000 at 1033.713 kb on + strand, within PGA1_c10000 at 1033.725 kb on + strand at 1033.799 kb on - strand at 1033.888 kb on - strand at 1033.907 kb on + strand at 1033.921 kb on - strand at 1033.956 kb on - strand at 1034.018 kb on - strand at 1034.071 kb on + strand at 1034.071 kb on + strand at 1034.079 kb on - strand at 1034.127 kb on - strand at 1034.132 kb on - strand, within PGA1_c10010 at 1034.181 kb on - strand, within PGA1_c10010 at 1034.198 kb on + strand, within PGA1_c10010 at 1034.198 kb on + strand, within PGA1_c10010 at 1034.200 kb on - strand, within PGA1_c10010 at 1034.232 kb on + strand, within PGA1_c10010 at 1034.244 kb on + strand, within PGA1_c10010 at 1034.258 kb on + strand, within PGA1_c10010 at 1034.266 kb on - strand, within PGA1_c10010 at 1034.266 kb on - strand, within PGA1_c10010 at 1034.266 kb on - strand, within PGA1_c10010 at 1034.311 kb on + strand, within PGA1_c10010 at 1034.311 kb on + strand, within PGA1_c10010 at 1034.319 kb on - strand, within PGA1_c10010 at 1034.319 kb on - strand, within PGA1_c10010 at 1034.365 kb on + strand, within PGA1_c10010 at 1034.398 kb on + strand, within PGA1_c10010 at 1034.400 kb on + strand, within PGA1_c10010 at 1034.404 kb on + strand, within PGA1_c10010 at 1034.404 kb on + strand, within PGA1_c10010 at 1034.412 kb on - strand, within PGA1_c10010 at 1034.433 kb on + strand, within PGA1_c10010 at 1034.436 kb on + strand, within PGA1_c10010 at 1034.444 kb on - strand, within PGA1_c10010 at 1034.468 kb on + strand, within PGA1_c10010 at 1034.507 kb on - strand, within PGA1_c10010 at 1034.507 kb on - strand, within PGA1_c10010 at 1034.547 kb on + strand, within PGA1_c10010 at 1034.623 kb on + strand, within PGA1_c10010 at 1034.623 kb on + strand, within PGA1_c10010 at 1034.623 kb on + strand, within PGA1_c10010 at 1034.623 kb on + strand, within PGA1_c10010 at 1034.623 kb on + strand, within PGA1_c10010 at 1034.623 kb on + strand, within PGA1_c10010 at 1034.623 kb on + strand, within PGA1_c10010 at 1034.623 kb on + strand, within PGA1_c10010 at 1034.631 kb on - strand, within PGA1_c10010 at 1034.631 kb on - strand, within PGA1_c10010 at 1034.709 kb on - strand, within PGA1_c10010 at 1034.709 kb on - strand, within PGA1_c10010 at 1034.709 kb on - strand, within PGA1_c10010 at 1034.709 kb on - strand, within PGA1_c10010 at 1034.709 kb on - strand, within PGA1_c10010 at 1034.709 kb on - strand, within PGA1_c10010 at 1034.709 kb on - strand, within PGA1_c10010 at 1034.710 kb on - strand, within PGA1_c10010 at 1034.723 kb on - strand, within PGA1_c10010 at 1034.742 kb on - strand, within PGA1_c10010 at 1034.747 kb on - strand, within PGA1_c10010 at 1034.756 kb on - strand, within PGA1_c10010 at 1034.761 kb on - strand, within PGA1_c10010 at 1034.763 kb on + strand, within PGA1_c10010 at 1034.782 kb on - strand, within PGA1_c10010
Per-strain Table
Position Strand Gene LocusTag Fraction Phaeo_ML1_MIT diversity barseq remove 1,032,040 + PGA1_c09980 0.58 +1.9 1,032,061 + PGA1_c09980 0.59 +1.3 1,032,076 + PGA1_c09980 0.61 -0.4 1,032,095 + PGA1_c09980 0.62 +0.6 1,032,113 + PGA1_c09980 0.64 -1.0 1,032,123 + PGA1_c09980 0.65 -0.3 1,032,149 + PGA1_c09980 0.67 -1.0 1,032,163 - PGA1_c09980 0.68 +0.2 1,032,180 + PGA1_c09980 0.69 +4.1 1,032,180 + PGA1_c09980 0.69 +0.4 1,032,188 - PGA1_c09980 0.70 -0.2 1,032,279 - PGA1_c09980 0.78 -1.0 1,032,325 + PGA1_c09980 0.81 -0.7 1,032,333 - PGA1_c09980 0.82 +2.6 1,032,387 + PGA1_c09980 0.87 -0.8 1,032,387 + PGA1_c09980 0.87 -0.4 1,032,390 - PGA1_c09980 0.87 -1.4 1,032,390 - PGA1_c09980 0.87 -0.7 1,032,405 - PGA1_c09980 0.88 -0.9 1,032,487 + +1.0 1,032,487 + +3.4 1,032,543 + +1.6 1,032,543 + +0.3 1,032,545 + -0.7 1,032,680 + PGA1_c09990 0.10 +0.7 1,032,735 + PGA1_c09990 0.24 +1.6 1,032,744 - PGA1_c09990 0.26 -0.6 1,032,744 - PGA1_c09990 0.26 +2.4 1,032,744 - PGA1_c09990 0.26 +2.1 1,032,745 + PGA1_c09990 0.27 +0.6 1,032,750 + PGA1_c09990 0.28 -0.4 1,032,779 + PGA1_c09990 0.35 +0.4 1,032,959 + PGA1_c09990 0.80 -0.4 1,032,991 - PGA1_c09990 0.88 +0.7 1,032,991 - PGA1_c09990 0.88 -0.0 1,033,033 - +0.7 1,033,040 + -0.2 1,033,040 + -0.7 1,033,089 - -1.0 1,033,144 - PGA1_c10000 0.14 +0.3 1,033,165 + PGA1_c10000 0.17 -0.1 1,033,197 + PGA1_c10000 0.21 +0.4 1,033,207 + PGA1_c10000 0.23 -0.9 1,033,281 + PGA1_c10000 0.32 +1.5 1,033,283 - PGA1_c10000 0.33 +0.0 1,033,321 - PGA1_c10000 0.38 -2.6 1,033,335 - PGA1_c10000 0.40 -0.9 1,033,378 - PGA1_c10000 0.45 -0.6 1,033,406 + PGA1_c10000 0.49 -0.4 1,033,438 + PGA1_c10000 0.53 +0.9 1,033,514 - PGA1_c10000 0.63 -0.0 1,033,526 - PGA1_c10000 0.65 +0.8 1,033,526 - PGA1_c10000 0.65 -0.4 1,033,526 - PGA1_c10000 0.65 -0.7 1,033,526 - PGA1_c10000 0.65 -0.0 1,033,561 - PGA1_c10000 0.70 -1.1 1,033,561 - PGA1_c10000 0.70 -0.2 1,033,561 - PGA1_c10000 0.70 -0.1 1,033,561 - PGA1_c10000 0.70 -0.4 1,033,623 + PGA1_c10000 0.78 -0.0 1,033,669 + PGA1_c10000 0.84 +1.6 1,033,677 - PGA1_c10000 0.85 -0.8 1,033,713 + PGA1_c10000 0.90 -0.5 1,033,725 + +0.2 1,033,799 - -0.4 1,033,888 - -0.5 1,033,907 + -0.0 1,033,921 - +0.6 1,033,956 - -0.7 1,034,018 - +0.8 1,034,071 + +1.1 1,034,071 + +1.5 1,034,079 - +1.9 1,034,127 - -1.4 1,034,132 - PGA1_c10010 0.10 +0.8 1,034,181 - PGA1_c10010 0.15 -0.7 1,034,198 + PGA1_c10010 0.17 +1.7 1,034,198 + PGA1_c10010 0.17 -0.2 1,034,200 - PGA1_c10010 0.17 -0.8 1,034,232 + PGA1_c10010 0.21 +0.5 1,034,244 + PGA1_c10010 0.22 +0.4 1,034,258 + PGA1_c10010 0.23 +2.0 1,034,266 - PGA1_c10010 0.24 -0.7 1,034,266 - PGA1_c10010 0.24 +0.6 1,034,266 - PGA1_c10010 0.24 -0.4 1,034,311 + PGA1_c10010 0.29 -0.4 1,034,311 + PGA1_c10010 0.29 -1.0 1,034,319 - PGA1_c10010 0.30 +0.8 1,034,319 - PGA1_c10010 0.30 +0.7 1,034,365 + PGA1_c10010 0.34 -0.3 1,034,398 + PGA1_c10010 0.38 +0.0 1,034,400 + PGA1_c10010 0.38 +0.1 1,034,404 + PGA1_c10010 0.39 +0.4 1,034,404 + PGA1_c10010 0.39 -0.0 1,034,412 - PGA1_c10010 0.39 +1.4 1,034,433 + PGA1_c10010 0.42 +0.1 1,034,436 + PGA1_c10010 0.42 +0.9 1,034,444 - PGA1_c10010 0.43 -0.9 1,034,468 + PGA1_c10010 0.45 +1.6 1,034,507 - PGA1_c10010 0.49 +0.0 1,034,507 - PGA1_c10010 0.49 +0.6 1,034,547 + PGA1_c10010 0.54 -0.1 1,034,623 + PGA1_c10010 0.62 +0.3 1,034,623 + PGA1_c10010 0.62 -0.6 1,034,623 + PGA1_c10010 0.62 +0.5 1,034,623 + PGA1_c10010 0.62 -0.9 1,034,623 + PGA1_c10010 0.62 +0.1 1,034,623 + PGA1_c10010 0.62 -0.1 1,034,623 + PGA1_c10010 0.62 -0.3 1,034,623 + PGA1_c10010 0.62 -0.6 1,034,631 - PGA1_c10010 0.62 +0.6 1,034,631 - PGA1_c10010 0.62 -0.4 1,034,709 - PGA1_c10010 0.71 +1.2 1,034,709 - PGA1_c10010 0.71 -0.4 1,034,709 - PGA1_c10010 0.71 +0.1 1,034,709 - PGA1_c10010 0.71 +0.5 1,034,709 - PGA1_c10010 0.71 +0.3 1,034,709 - PGA1_c10010 0.71 -0.4 1,034,709 - PGA1_c10010 0.71 -0.1 1,034,710 - PGA1_c10010 0.71 -0.4 1,034,723 - PGA1_c10010 0.72 -1.4 1,034,742 - PGA1_c10010 0.74 -0.7 1,034,747 - PGA1_c10010 0.75 -0.6 1,034,756 - PGA1_c10010 0.75 +0.8 1,034,761 - PGA1_c10010 0.76 +0.1 1,034,763 + PGA1_c10010 0.76 -0.4 1,034,782 - PGA1_c10010 0.78 +0.6
Or see this region's nucleotide sequence