Experiment: Phaeo_ML1_MIT diversity barseq
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c09930 and PGA1_c09950 are separated by 4 nucleotides PGA1_c09950 and PGA1_c09960 are separated by 99 nucleotides PGA1_c09960 and PGA1_c09970 are separated by 38 nucleotides
PGA1_c09930: PGA1_c09930 - isoquinoline 1-oxidoreductase subunit beta, at 1,026,937 to 1,029,177
_c09930
PGA1_c09950: PGA1_c09950 - isoquinoline 1-oxidoreductase subunit alpha, at 1,029,182 to 1,029,646
_c09950
PGA1_c09960: PGA1_c09960 - putative transcriptional regulator, TetR family, at 1,029,746 to 1,030,327
_c09960
PGA1_c09970: PGA1_c09970 - tryptophan 2,3-dioxygenase KynA, at 1,030,366 to 1,031,199
_c09970
Position (kb)
1029
1030
1031 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1028.839 kb on + strand, within PGA1_c09930 at 1028.850 kb on + strand, within PGA1_c09930 at 1028.858 kb on - strand, within PGA1_c09930 at 1028.886 kb on - strand, within PGA1_c09930 at 1028.896 kb on + strand, within PGA1_c09930 at 1028.896 kb on - strand, within PGA1_c09930 at 1028.987 kb on - strand at 1028.990 kb on + strand at 1029.000 kb on + strand at 1029.032 kb on + strand at 1029.034 kb on + strand at 1029.260 kb on + strand, within PGA1_c09950 at 1029.303 kb on - strand, within PGA1_c09950 at 1029.340 kb on - strand, within PGA1_c09950 at 1029.355 kb on - strand, within PGA1_c09950 at 1029.378 kb on + strand, within PGA1_c09950 at 1029.378 kb on + strand, within PGA1_c09950 at 1029.400 kb on + strand, within PGA1_c09950 at 1029.422 kb on - strand, within PGA1_c09950 at 1029.424 kb on + strand, within PGA1_c09950 at 1029.474 kb on - strand, within PGA1_c09950 at 1029.601 kb on + strand at 1029.629 kb on - strand at 1029.910 kb on + strand, within PGA1_c09960 at 1029.917 kb on + strand, within PGA1_c09960 at 1029.945 kb on + strand, within PGA1_c09960 at 1029.965 kb on + strand, within PGA1_c09960 at 1029.981 kb on - strand, within PGA1_c09960 at 1030.123 kb on - strand, within PGA1_c09960 at 1030.202 kb on - strand, within PGA1_c09960 at 1030.248 kb on + strand, within PGA1_c09960 at 1030.322 kb on + strand at 1030.448 kb on - strand at 1030.590 kb on - strand, within PGA1_c09970 at 1030.625 kb on + strand, within PGA1_c09970 at 1030.625 kb on - strand, within PGA1_c09970 at 1030.633 kb on - strand, within PGA1_c09970 at 1030.680 kb on + strand, within PGA1_c09970 at 1030.688 kb on - strand, within PGA1_c09970 at 1030.706 kb on - strand, within PGA1_c09970 at 1030.741 kb on + strand, within PGA1_c09970 at 1030.741 kb on + strand, within PGA1_c09970 at 1030.749 kb on - strand, within PGA1_c09970 at 1030.781 kb on + strand, within PGA1_c09970 at 1030.819 kb on + strand, within PGA1_c09970 at 1030.827 kb on - strand, within PGA1_c09970 at 1030.827 kb on - strand, within PGA1_c09970 at 1030.875 kb on + strand, within PGA1_c09970 at 1030.913 kb on + strand, within PGA1_c09970 at 1030.943 kb on + strand, within PGA1_c09970 at 1030.948 kb on - strand, within PGA1_c09970 at 1030.998 kb on - strand, within PGA1_c09970 at 1031.034 kb on + strand, within PGA1_c09970 at 1031.034 kb on + strand, within PGA1_c09970 at 1031.034 kb on + strand, within PGA1_c09970 at 1031.137 kb on + strand at 1031.145 kb on - strand at 1031.180 kb on + strand at 1031.213 kb on - strand at 1031.227 kb on + strand at 1031.235 kb on - strand at 1031.259 kb on + strand at 1031.303 kb on - strand at 1031.315 kb on + strand at 1031.325 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Phaeo_ML1_MIT diversity barseq remove 1,028,839 + PGA1_c09930 0.85 -0.6 1,028,850 + PGA1_c09930 0.85 -0.8 1,028,858 - PGA1_c09930 0.86 -0.6 1,028,886 - PGA1_c09930 0.87 -0.3 1,028,896 + PGA1_c09930 0.87 -0.3 1,028,896 - PGA1_c09930 0.87 -0.3 1,028,987 - -0.2 1,028,990 + +0.0 1,029,000 + +0.1 1,029,032 + +1.6 1,029,034 + +0.5 1,029,260 + PGA1_c09950 0.17 +1.3 1,029,303 - PGA1_c09950 0.26 +0.6 1,029,340 - PGA1_c09950 0.34 -1.0 1,029,355 - PGA1_c09950 0.37 +0.9 1,029,378 + PGA1_c09950 0.42 +1.2 1,029,378 + PGA1_c09950 0.42 -1.5 1,029,400 + PGA1_c09950 0.47 +0.2 1,029,422 - PGA1_c09950 0.52 -0.7 1,029,424 + PGA1_c09950 0.52 +1.1 1,029,474 - PGA1_c09950 0.63 -0.7 1,029,601 + +0.1 1,029,629 - +1.2 1,029,910 + PGA1_c09960 0.28 +0.2 1,029,917 + PGA1_c09960 0.29 -1.0 1,029,945 + PGA1_c09960 0.34 +0.4 1,029,965 + PGA1_c09960 0.38 -0.1 1,029,981 - PGA1_c09960 0.40 -1.7 1,030,123 - PGA1_c09960 0.65 +0.6 1,030,202 - PGA1_c09960 0.78 +1.9 1,030,248 + PGA1_c09960 0.86 -0.3 1,030,322 + -1.2 1,030,448 - +0.9 1,030,590 - PGA1_c09970 0.27 +3.1 1,030,625 + PGA1_c09970 0.31 -0.8 1,030,625 - PGA1_c09970 0.31 -0.8 1,030,633 - PGA1_c09970 0.32 +1.3 1,030,680 + PGA1_c09970 0.38 -2.0 1,030,688 - PGA1_c09970 0.39 +0.2 1,030,706 - PGA1_c09970 0.41 -0.8 1,030,741 + PGA1_c09970 0.45 -0.5 1,030,741 + PGA1_c09970 0.45 -1.7 1,030,749 - PGA1_c09970 0.46 -2.8 1,030,781 + PGA1_c09970 0.50 -0.3 1,030,819 + PGA1_c09970 0.54 -0.8 1,030,827 - PGA1_c09970 0.55 -0.6 1,030,827 - PGA1_c09970 0.55 +0.2 1,030,875 + PGA1_c09970 0.61 -0.6 1,030,913 + PGA1_c09970 0.66 +0.2 1,030,943 + PGA1_c09970 0.69 -0.2 1,030,948 - PGA1_c09970 0.70 -0.1 1,030,998 - PGA1_c09970 0.76 +0.2 1,031,034 + PGA1_c09970 0.80 -0.2 1,031,034 + PGA1_c09970 0.80 -1.4 1,031,034 + PGA1_c09970 0.80 -0.4 1,031,137 + +0.4 1,031,145 - -0.3 1,031,180 + -0.5 1,031,213 - -1.3 1,031,227 + +0.2 1,031,235 - -0.5 1,031,259 + -0.0 1,031,303 - +1.1 1,031,315 + +1.1 1,031,325 - -1.0
Or see this region's nucleotide sequence