Experiment: Phaeo_ML1_MIT diversity barseq
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c08820 and PGA1_c08830 are separated by 254 nucleotides PGA1_c08830 and PGA1_c08840 are separated by 33 nucleotides PGA1_c08840 and PGA1_c08850 are separated by 40 nucleotides
PGA1_c08820: PGA1_c08820 - putative oxidoreductase, at 912,215 to 912,889
_c08820
PGA1_c08830: PGA1_c08830 - hypothetical protein, at 913,144 to 913,554
_c08830
PGA1_c08840: PGA1_c08840 - hypothetical protein, at 913,588 to 914,079
_c08840
PGA1_c08850: PGA1_c08850 - secondary thiamine-phosphate synthase enzyme, at 914,120 to 914,536
_c08850
Position (kb) 913
914 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 912.168 kb on - strand at 912.172 kb on + strand at 912.218 kb on + strand at 912.389 kb on - strand, within PGA1_c08820 at 912.496 kb on - strand, within PGA1_c08820 at 912.505 kb on - strand, within PGA1_c08820 at 912.528 kb on + strand, within PGA1_c08820 at 912.543 kb on + strand, within PGA1_c08820 at 912.543 kb on + strand, within PGA1_c08820 at 912.543 kb on + strand, within PGA1_c08820 at 912.543 kb on + strand, within PGA1_c08820 at 912.551 kb on - strand, within PGA1_c08820 at 912.551 kb on - strand, within PGA1_c08820 at 912.551 kb on - strand, within PGA1_c08820 at 912.620 kb on + strand, within PGA1_c08820 at 912.628 kb on - strand, within PGA1_c08820 at 912.730 kb on + strand, within PGA1_c08820 at 912.730 kb on + strand, within PGA1_c08820 at 912.730 kb on + strand, within PGA1_c08820 at 912.738 kb on - strand, within PGA1_c08820 at 912.757 kb on + strand, within PGA1_c08820 at 912.823 kb on - strand at 912.823 kb on - strand at 912.849 kb on - strand at 912.940 kb on - strand at 912.963 kb on + strand at 912.968 kb on + strand at 912.987 kb on + strand at 913.046 kb on + strand at 913.046 kb on - strand at 913.066 kb on - strand at 913.088 kb on + strand at 913.090 kb on + strand at 913.110 kb on - strand at 913.138 kb on + strand at 913.146 kb on - strand at 913.160 kb on - strand at 913.191 kb on - strand, within PGA1_c08830 at 913.191 kb on - strand, within PGA1_c08830 at 913.191 kb on - strand, within PGA1_c08830 at 913.193 kb on + strand, within PGA1_c08830 at 913.251 kb on + strand, within PGA1_c08830 at 913.383 kb on + strand, within PGA1_c08830 at 913.383 kb on + strand, within PGA1_c08830 at 913.391 kb on + strand, within PGA1_c08830 at 913.391 kb on - strand, within PGA1_c08830 at 913.392 kb on - strand, within PGA1_c08830 at 913.427 kb on + strand, within PGA1_c08830 at 913.471 kb on - strand, within PGA1_c08830 at 913.500 kb on + strand, within PGA1_c08830 at 913.513 kb on - strand, within PGA1_c08830 at 913.545 kb on - strand at 913.627 kb on + strand at 913.627 kb on + strand at 913.635 kb on - strand at 913.650 kb on - strand, within PGA1_c08840 at 913.707 kb on + strand, within PGA1_c08840 at 913.715 kb on - strand, within PGA1_c08840 at 913.725 kb on - strand, within PGA1_c08840 at 913.780 kb on - strand, within PGA1_c08840 at 913.855 kb on - strand, within PGA1_c08840 at 913.865 kb on - strand, within PGA1_c08840 at 913.880 kb on - strand, within PGA1_c08840 at 913.880 kb on - strand, within PGA1_c08840 at 913.937 kb on + strand, within PGA1_c08840 at 913.987 kb on + strand, within PGA1_c08840 at 914.016 kb on - strand, within PGA1_c08840 at 914.075 kb on + strand at 914.081 kb on - strand at 914.096 kb on - strand at 914.109 kb on + strand at 914.109 kb on + strand at 914.117 kb on - strand at 914.117 kb on - strand at 914.140 kb on + strand at 914.151 kb on + strand at 914.153 kb on - strand at 914.159 kb on - strand at 914.200 kb on - strand, within PGA1_c08850 at 914.204 kb on + strand, within PGA1_c08850 at 914.209 kb on + strand, within PGA1_c08850 at 914.211 kb on + strand, within PGA1_c08850 at 914.211 kb on + strand, within PGA1_c08850 at 914.219 kb on - strand, within PGA1_c08850 at 914.219 kb on - strand, within PGA1_c08850 at 914.244 kb on + strand, within PGA1_c08850 at 914.395 kb on + strand, within PGA1_c08850 at 914.403 kb on - strand, within PGA1_c08850 at 914.453 kb on + strand, within PGA1_c08850 at 914.461 kb on - strand, within PGA1_c08850 at 914.474 kb on - strand, within PGA1_c08850 at 914.481 kb on - strand, within PGA1_c08850 at 914.508 kb on + strand at 914.508 kb on + strand at 914.508 kb on + strand at 914.508 kb on + strand at 914.508 kb on + strand at 914.508 kb on + strand at 914.508 kb on + strand at 914.508 kb on + strand at 914.508 kb on + strand at 914.508 kb on + strand at 914.508 kb on + strand at 914.508 kb on + strand at 914.508 kb on + strand at 914.508 kb on + strand at 914.508 kb on + strand at 914.508 kb on + strand at 914.508 kb on + strand at 914.516 kb on - strand at 914.516 kb on - strand at 914.516 kb on - strand at 914.516 kb on - strand at 914.516 kb on - strand at 914.516 kb on - strand at 914.516 kb on - strand at 914.530 kb on + strand at 914.538 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Phaeo_ML1_MIT diversity barseq remove 912,168 - -0.4 912,172 + +0.2 912,218 + +0.6 912,389 - PGA1_c08820 0.26 +2.1 912,496 - PGA1_c08820 0.42 +0.2 912,505 - PGA1_c08820 0.43 +0.1 912,528 + PGA1_c08820 0.46 +0.0 912,543 + PGA1_c08820 0.49 -1.0 912,543 + PGA1_c08820 0.49 -0.9 912,543 + PGA1_c08820 0.49 -0.2 912,543 + PGA1_c08820 0.49 +0.0 912,551 - PGA1_c08820 0.50 +1.6 912,551 - PGA1_c08820 0.50 +0.2 912,551 - PGA1_c08820 0.50 -0.3 912,620 + PGA1_c08820 0.60 +0.0 912,628 - PGA1_c08820 0.61 +0.1 912,730 + PGA1_c08820 0.76 -2.0 912,730 + PGA1_c08820 0.76 +0.6 912,730 + PGA1_c08820 0.76 -1.1 912,738 - PGA1_c08820 0.77 +1.1 912,757 + PGA1_c08820 0.80 +1.0 912,823 - +1.6 912,823 - +1.9 912,849 - +0.3 912,940 - +0.4 912,963 + +0.9 912,968 + +0.9 912,987 + +1.7 913,046 + +0.6 913,046 - +0.7 913,066 - -0.7 913,088 + +0.1 913,090 + -0.4 913,110 - -0.3 913,138 + +1.3 913,146 - +1.4 913,160 - +1.1 913,191 - PGA1_c08830 0.11 -0.6 913,191 - PGA1_c08830 0.11 +0.2 913,191 - PGA1_c08830 0.11 -2.1 913,193 + PGA1_c08830 0.12 -0.3 913,251 + PGA1_c08830 0.26 +0.6 913,383 + PGA1_c08830 0.58 -0.7 913,383 + PGA1_c08830 0.58 +0.6 913,391 + PGA1_c08830 0.60 -1.1 913,391 - PGA1_c08830 0.60 +0.3 913,392 - PGA1_c08830 0.60 -1.1 913,427 + PGA1_c08830 0.69 +0.2 913,471 - PGA1_c08830 0.80 -0.4 913,500 + PGA1_c08830 0.87 -1.3 913,513 - PGA1_c08830 0.90 -0.9 913,545 - +2.3 913,627 + +1.8 913,627 + -0.9 913,635 - -0.5 913,650 - PGA1_c08840 0.13 -0.5 913,707 + PGA1_c08840 0.24 -0.6 913,715 - PGA1_c08840 0.26 +3.2 913,725 - PGA1_c08840 0.28 +1.3 913,780 - PGA1_c08840 0.39 +0.6 913,855 - PGA1_c08840 0.54 +1.2 913,865 - PGA1_c08840 0.56 -0.8 913,880 - PGA1_c08840 0.59 +1.0 913,880 - PGA1_c08840 0.59 -0.9 913,937 + PGA1_c08840 0.71 +0.4 913,987 + PGA1_c08840 0.81 +1.0 914,016 - PGA1_c08840 0.87 +0.1 914,075 + -0.4 914,081 - -0.2 914,096 - -0.7 914,109 + -1.0 914,109 + +0.3 914,117 - -0.2 914,117 - +0.6 914,140 + -0.8 914,151 + -0.3 914,153 - +0.4 914,159 - -0.8 914,200 - PGA1_c08850 0.19 -0.8 914,204 + PGA1_c08850 0.20 +0.1 914,209 + PGA1_c08850 0.21 +0.7 914,211 + PGA1_c08850 0.22 +0.3 914,211 + PGA1_c08850 0.22 +1.1 914,219 - PGA1_c08850 0.24 +2.1 914,219 - PGA1_c08850 0.24 +0.1 914,244 + PGA1_c08850 0.30 +1.1 914,395 + PGA1_c08850 0.66 +0.1 914,403 - PGA1_c08850 0.68 -0.9 914,453 + PGA1_c08850 0.80 +1.0 914,461 - PGA1_c08850 0.82 -0.9 914,474 - PGA1_c08850 0.85 +0.4 914,481 - PGA1_c08850 0.87 +0.0 914,508 + -0.9 914,508 + +0.2 914,508 + +0.1 914,508 + -0.3 914,508 + -0.8 914,508 + -0.4 914,508 + +0.9 914,508 + +0.6 914,508 + -0.3 914,508 + -0.4 914,508 + +1.1 914,508 + +0.6 914,508 + +1.1 914,508 + -0.8 914,508 + -0.4 914,508 + -0.2 914,508 + +0.9 914,516 - +0.0 914,516 - +2.9 914,516 - +1.6 914,516 - +1.1 914,516 - +0.9 914,516 - +2.6 914,516 - +0.2 914,530 + +0.2 914,538 - -1.2
Or see this region's nucleotide sequence