Experiment: Phaeo_ML1_MIT diversity barseq
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c06470 and PGA1_c06480 are separated by 33 nucleotides PGA1_c06480 and PGA1_c06490 are separated by 121 nucleotides PGA1_c06490 and PGA1_c06500 are separated by 9 nucleotides
PGA1_c06470: PGA1_c06470 - glycyl-tRNA synthetase alpha subunit, at 662,267 to 663,196
_c06470
PGA1_c06480: PGA1_c06480 - hypothetical protein, at 663,230 to 663,763
_c06480
PGA1_c06490: PGA1_c06490 - sodium/glutamate symport carrier protein GltS, at 663,885 to 665,093
_c06490
PGA1_c06500: PGA1_c06500 - glycyl-tRNA synthetase beta subunit, at 665,103 to 667,367
_c06500
Position (kb) 663
664
665
666 Strain fitness (log2 ratio) -2
-1
0
1
2 at 663.191 kb on + strand at 663.227 kb on + strand at 663.302 kb on - strand, within PGA1_c06480 at 663.698 kb on + strand, within PGA1_c06480 at 663.698 kb on + strand, within PGA1_c06480 at 663.729 kb on + strand at 663.757 kb on - strand at 663.771 kb on + strand at 663.801 kb on - strand at 663.834 kb on - strand at 663.861 kb on + strand at 663.861 kb on + strand at 663.861 kb on + strand at 663.861 kb on + strand at 663.869 kb on - strand at 663.896 kb on + strand at 663.896 kb on + strand at 663.904 kb on - strand at 663.904 kb on - strand at 663.904 kb on - strand at 663.904 kb on - strand at 663.904 kb on - strand at 663.966 kb on - strand at 663.978 kb on + strand at 663.978 kb on + strand at 664.046 kb on + strand, within PGA1_c06490 at 664.054 kb on + strand, within PGA1_c06490 at 664.062 kb on - strand, within PGA1_c06490 at 664.285 kb on - strand, within PGA1_c06490 at 664.303 kb on - strand, within PGA1_c06490 at 664.350 kb on + strand, within PGA1_c06490 at 664.390 kb on + strand, within PGA1_c06490 at 664.557 kb on - strand, within PGA1_c06490 at 664.605 kb on - strand, within PGA1_c06490 at 664.605 kb on - strand, within PGA1_c06490 at 664.725 kb on - strand, within PGA1_c06490 at 664.787 kb on + strand, within PGA1_c06490 at 664.797 kb on + strand, within PGA1_c06490 at 664.809 kb on + strand, within PGA1_c06490 at 664.809 kb on + strand, within PGA1_c06490 at 664.812 kb on - strand, within PGA1_c06490 at 664.843 kb on - strand, within PGA1_c06490 at 664.843 kb on - strand, within PGA1_c06490 at 664.843 kb on - strand, within PGA1_c06490 at 664.866 kb on - strand, within PGA1_c06490 at 664.933 kb on + strand, within PGA1_c06490 at 664.941 kb on - strand, within PGA1_c06490 at 664.941 kb on - strand, within PGA1_c06490 at 664.985 kb on - strand at 664.990 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Phaeo_ML1_MIT diversity barseq remove 663,191 + +1.2 663,227 + -0.1 663,302 - PGA1_c06480 0.13 -2.4 663,698 + PGA1_c06480 0.88 -0.8 663,698 + PGA1_c06480 0.88 +0.4 663,729 + -0.4 663,757 - -0.5 663,771 + -0.2 663,801 - +0.6 663,834 - -0.4 663,861 + -0.8 663,861 + +0.2 663,861 + -0.4 663,861 + -1.0 663,869 - +1.8 663,896 + -0.9 663,896 + -0.1 663,904 - +0.6 663,904 - +1.2 663,904 - +2.2 663,904 - +1.8 663,904 - -1.0 663,966 - -0.9 663,978 + -0.0 663,978 + -1.0 664,046 + PGA1_c06490 0.13 +0.2 664,054 + PGA1_c06490 0.14 -2.0 664,062 - PGA1_c06490 0.15 -1.0 664,285 - PGA1_c06490 0.33 -0.8 664,303 - PGA1_c06490 0.35 -2.0 664,350 + PGA1_c06490 0.38 +2.0 664,390 + PGA1_c06490 0.42 -1.0 664,557 - PGA1_c06490 0.56 -0.8 664,605 - PGA1_c06490 0.60 -1.5 664,605 - PGA1_c06490 0.60 +1.9 664,725 - PGA1_c06490 0.69 -0.0 664,787 + PGA1_c06490 0.75 -0.3 664,797 + PGA1_c06490 0.75 -1.7 664,809 + PGA1_c06490 0.76 -0.0 664,809 + PGA1_c06490 0.76 -0.2 664,812 - PGA1_c06490 0.77 +0.7 664,843 - PGA1_c06490 0.79 -0.9 664,843 - PGA1_c06490 0.79 +0.2 664,843 - PGA1_c06490 0.79 -0.1 664,866 - PGA1_c06490 0.81 +1.2 664,933 + PGA1_c06490 0.87 -0.4 664,941 - PGA1_c06490 0.87 -1.2 664,941 - PGA1_c06490 0.87 -2.0 664,985 - +0.6 664,990 + -1.0
Or see this region's nucleotide sequence