Experiment: Phaeo_ML1_MIT diversity barseq
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c06080 and PGA1_c06090 are separated by 3 nucleotides PGA1_c06090 and PGA1_c06100 are separated by 10 nucleotides PGA1_c06100 and PGA1_c06110 overlap by 1 nucleotides
PGA1_c06080: PGA1_c06080 - hypothetical protein, at 622,261 to 622,863
_c06080
PGA1_c06090: PGA1_c06090 - type II secretion system protein F-like protein, at 622,867 to 623,847
_c06090
PGA1_c06100: PGA1_c06100 - type II secretion system protein F-like protein, at 623,858 to 624,826
_c06100
PGA1_c06110: PGA1_c06110 - Flp pilus assembly protein, ATPase CpaF, at 624,826 to 626,271
_c06110
Position (kb) 623
624
625 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 622.895 kb on - strand at 622.900 kb on - strand at 622.905 kb on - strand at 622.918 kb on - strand at 622.941 kb on + strand at 622.949 kb on - strand at 623.007 kb on + strand, within PGA1_c06090 at 623.033 kb on + strand, within PGA1_c06090 at 623.033 kb on + strand, within PGA1_c06090 at 623.033 kb on + strand, within PGA1_c06090 at 623.041 kb on - strand, within PGA1_c06090 at 623.041 kb on - strand, within PGA1_c06090 at 623.041 kb on - strand, within PGA1_c06090 at 623.041 kb on - strand, within PGA1_c06090 at 623.041 kb on - strand, within PGA1_c06090 at 623.128 kb on - strand, within PGA1_c06090 at 623.153 kb on + strand, within PGA1_c06090 at 623.222 kb on - strand, within PGA1_c06090 at 623.244 kb on - strand, within PGA1_c06090 at 623.256 kb on + strand, within PGA1_c06090 at 623.292 kb on - strand, within PGA1_c06090 at 623.374 kb on + strand, within PGA1_c06090 at 623.381 kb on - strand, within PGA1_c06090 at 623.425 kb on + strand, within PGA1_c06090 at 623.456 kb on + strand, within PGA1_c06090 at 623.464 kb on - strand, within PGA1_c06090 at 623.466 kb on + strand, within PGA1_c06090 at 623.474 kb on - strand, within PGA1_c06090 at 623.487 kb on + strand, within PGA1_c06090 at 623.502 kb on + strand, within PGA1_c06090 at 623.567 kb on + strand, within PGA1_c06090 at 623.575 kb on - strand, within PGA1_c06090 at 623.577 kb on + strand, within PGA1_c06090 at 623.577 kb on + strand, within PGA1_c06090 at 623.640 kb on - strand, within PGA1_c06090 at 623.668 kb on + strand, within PGA1_c06090 at 623.671 kb on - strand, within PGA1_c06090 at 623.676 kb on - strand, within PGA1_c06090 at 623.724 kb on + strand, within PGA1_c06090 at 623.798 kb on + strand at 623.808 kb on - strand at 623.904 kb on - strand at 623.940 kb on + strand at 624.031 kb on + strand, within PGA1_c06100 at 624.054 kb on + strand, within PGA1_c06100 at 624.079 kb on + strand, within PGA1_c06100 at 624.099 kb on + strand, within PGA1_c06100 at 624.121 kb on + strand, within PGA1_c06100 at 624.160 kb on + strand, within PGA1_c06100 at 624.168 kb on + strand, within PGA1_c06100 at 624.285 kb on + strand, within PGA1_c06100 at 624.425 kb on + strand, within PGA1_c06100 at 624.478 kb on + strand, within PGA1_c06100 at 624.569 kb on + strand, within PGA1_c06100 at 624.617 kb on - strand, within PGA1_c06100 at 624.727 kb on - strand, within PGA1_c06100 at 624.751 kb on + strand at 624.767 kb on - strand at 624.786 kb on - strand at 624.808 kb on + strand at 624.821 kb on - strand at 624.879 kb on - strand at 625.064 kb on + strand, within PGA1_c06110 at 625.184 kb on + strand, within PGA1_c06110 at 625.283 kb on - strand, within PGA1_c06110 at 625.482 kb on - strand, within PGA1_c06110 at 625.511 kb on - strand, within PGA1_c06110 at 625.559 kb on - strand, within PGA1_c06110 at 625.576 kb on + strand, within PGA1_c06110 at 625.576 kb on + strand, within PGA1_c06110 at 625.586 kb on + strand, within PGA1_c06110 at 625.597 kb on - strand, within PGA1_c06110 at 625.783 kb on + strand, within PGA1_c06110 at 625.795 kb on + strand, within PGA1_c06110
Per-strain Table
Position Strand Gene LocusTag Fraction Phaeo_ML1_MIT diversity barseq remove 622,895 - +0.3 622,900 - +1.5 622,905 - -0.3 622,918 - -0.1 622,941 + -0.6 622,949 - -0.0 623,007 + PGA1_c06090 0.14 -1.3 623,033 + PGA1_c06090 0.17 -0.8 623,033 + PGA1_c06090 0.17 -1.5 623,033 + PGA1_c06090 0.17 +0.1 623,041 - PGA1_c06090 0.18 -1.3 623,041 - PGA1_c06090 0.18 +0.1 623,041 - PGA1_c06090 0.18 +2.4 623,041 - PGA1_c06090 0.18 -2.0 623,041 - PGA1_c06090 0.18 +0.3 623,128 - PGA1_c06090 0.27 -1.0 623,153 + PGA1_c06090 0.29 -0.9 623,222 - PGA1_c06090 0.36 -1.0 623,244 - PGA1_c06090 0.38 -0.5 623,256 + PGA1_c06090 0.40 -0.7 623,292 - PGA1_c06090 0.43 -0.3 623,374 + PGA1_c06090 0.52 -1.8 623,381 - PGA1_c06090 0.52 +0.2 623,425 + PGA1_c06090 0.57 -0.0 623,456 + PGA1_c06090 0.60 +0.2 623,464 - PGA1_c06090 0.61 -0.3 623,466 + PGA1_c06090 0.61 -1.2 623,474 - PGA1_c06090 0.62 -0.3 623,487 + PGA1_c06090 0.63 -0.4 623,502 + PGA1_c06090 0.65 -1.3 623,567 + PGA1_c06090 0.71 +1.1 623,575 - PGA1_c06090 0.72 +2.9 623,577 + PGA1_c06090 0.72 -1.3 623,577 + PGA1_c06090 0.72 -1.8 623,640 - PGA1_c06090 0.79 +1.5 623,668 + PGA1_c06090 0.82 +0.0 623,671 - PGA1_c06090 0.82 -0.0 623,676 - PGA1_c06090 0.82 +0.1 623,724 + PGA1_c06090 0.87 +0.7 623,798 + -0.7 623,808 - +0.9 623,904 - -2.5 623,940 + -1.5 624,031 + PGA1_c06100 0.18 -0.3 624,054 + PGA1_c06100 0.20 +0.5 624,079 + PGA1_c06100 0.23 -0.8 624,099 + PGA1_c06100 0.25 -0.1 624,121 + PGA1_c06100 0.27 -1.0 624,160 + PGA1_c06100 0.31 -0.2 624,168 + PGA1_c06100 0.32 -1.5 624,285 + PGA1_c06100 0.44 +2.0 624,425 + PGA1_c06100 0.59 -0.6 624,478 + PGA1_c06100 0.64 +0.0 624,569 + PGA1_c06100 0.73 -1.6 624,617 - PGA1_c06100 0.78 -0.4 624,727 - PGA1_c06100 0.90 +0.5 624,751 + +0.2 624,767 - -0.3 624,786 - +0.0 624,808 + +1.0 624,821 - -1.2 624,879 - -1.5 625,064 + PGA1_c06110 0.16 +0.2 625,184 + PGA1_c06110 0.25 -0.7 625,283 - PGA1_c06110 0.32 -0.2 625,482 - PGA1_c06110 0.45 +1.1 625,511 - PGA1_c06110 0.47 +1.1 625,559 - PGA1_c06110 0.51 +1.2 625,576 + PGA1_c06110 0.52 +1.5 625,576 + PGA1_c06110 0.52 -1.3 625,586 + PGA1_c06110 0.53 -0.3 625,597 - PGA1_c06110 0.53 +0.8 625,783 + PGA1_c06110 0.66 -0.5 625,795 + PGA1_c06110 0.67 +0.5
Or see this region's nucleotide sequence