Experiment: Phaeo_ML1_MIT diversity barseq
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c06060 and PGA1_c06070 are separated by 19 nucleotides PGA1_c06070 and PGA1_c06080 are separated by 40 nucleotides PGA1_c06080 and PGA1_c06090 are separated by 3 nucleotides PGA1_c06090 and PGA1_c06100 are separated by 10 nucleotides
PGA1_c06060: PGA1_c06060 - Flp pilus assembly protein, protease CpaA, at 620,846 to 621,352
_c06060
PGA1_c06070: PGA1_c06070 - hypothetical protein, at 621,372 to 622,220
_c06070
PGA1_c06080: PGA1_c06080 - hypothetical protein, at 622,261 to 622,863
_c06080
PGA1_c06090: PGA1_c06090 - type II secretion system protein F-like protein, at 622,867 to 623,847
_c06090
PGA1_c06100: PGA1_c06100 - type II secretion system protein F-like protein, at 623,858 to 624,826
_c06100
Position (kb) 622
623 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 621.265 kb on - strand, within PGA1_c06060 at 621.294 kb on - strand, within PGA1_c06060 at 621.494 kb on + strand, within PGA1_c06070 at 621.534 kb on - strand, within PGA1_c06070 at 621.672 kb on - strand, within PGA1_c06070 at 621.674 kb on + strand, within PGA1_c06070 at 621.674 kb on + strand, within PGA1_c06070 at 621.682 kb on - strand, within PGA1_c06070 at 621.725 kb on - strand, within PGA1_c06070 at 621.737 kb on + strand, within PGA1_c06070 at 621.745 kb on - strand, within PGA1_c06070 at 621.745 kb on - strand, within PGA1_c06070 at 621.755 kb on - strand, within PGA1_c06070 at 621.831 kb on - strand, within PGA1_c06070 at 621.844 kb on + strand, within PGA1_c06070 at 621.852 kb on - strand, within PGA1_c06070 at 621.961 kb on - strand, within PGA1_c06070 at 621.966 kb on - strand, within PGA1_c06070 at 621.997 kb on + strand, within PGA1_c06070 at 622.004 kb on + strand, within PGA1_c06070 at 622.005 kb on - strand, within PGA1_c06070 at 622.040 kb on + strand, within PGA1_c06070 at 622.087 kb on - strand, within PGA1_c06070 at 622.155 kb on - strand at 622.172 kb on + strand at 622.230 kb on + strand at 622.235 kb on + strand at 622.245 kb on + strand at 622.253 kb on - strand at 622.253 kb on - strand at 622.355 kb on - strand, within PGA1_c06080 at 622.467 kb on + strand, within PGA1_c06080 at 622.505 kb on + strand, within PGA1_c06080 at 622.535 kb on + strand, within PGA1_c06080 at 622.553 kb on - strand, within PGA1_c06080 at 622.579 kb on - strand, within PGA1_c06080 at 622.601 kb on - strand, within PGA1_c06080 at 622.626 kb on + strand, within PGA1_c06080 at 622.635 kb on - strand, within PGA1_c06080 at 622.721 kb on - strand, within PGA1_c06080 at 622.721 kb on - strand, within PGA1_c06080 at 622.737 kb on - strand, within PGA1_c06080 at 622.844 kb on + strand at 622.895 kb on - strand at 622.900 kb on - strand at 622.905 kb on - strand at 622.918 kb on - strand at 622.941 kb on + strand at 622.949 kb on - strand at 623.007 kb on + strand, within PGA1_c06090 at 623.033 kb on + strand, within PGA1_c06090 at 623.033 kb on + strand, within PGA1_c06090 at 623.033 kb on + strand, within PGA1_c06090 at 623.041 kb on - strand, within PGA1_c06090 at 623.041 kb on - strand, within PGA1_c06090 at 623.041 kb on - strand, within PGA1_c06090 at 623.041 kb on - strand, within PGA1_c06090 at 623.041 kb on - strand, within PGA1_c06090 at 623.128 kb on - strand, within PGA1_c06090 at 623.153 kb on + strand, within PGA1_c06090 at 623.222 kb on - strand, within PGA1_c06090 at 623.244 kb on - strand, within PGA1_c06090 at 623.256 kb on + strand, within PGA1_c06090 at 623.292 kb on - strand, within PGA1_c06090 at 623.374 kb on + strand, within PGA1_c06090 at 623.381 kb on - strand, within PGA1_c06090 at 623.425 kb on + strand, within PGA1_c06090 at 623.456 kb on + strand, within PGA1_c06090 at 623.464 kb on - strand, within PGA1_c06090 at 623.466 kb on + strand, within PGA1_c06090 at 623.474 kb on - strand, within PGA1_c06090 at 623.487 kb on + strand, within PGA1_c06090 at 623.502 kb on + strand, within PGA1_c06090 at 623.567 kb on + strand, within PGA1_c06090 at 623.575 kb on - strand, within PGA1_c06090 at 623.577 kb on + strand, within PGA1_c06090 at 623.577 kb on + strand, within PGA1_c06090 at 623.640 kb on - strand, within PGA1_c06090 at 623.668 kb on + strand, within PGA1_c06090 at 623.671 kb on - strand, within PGA1_c06090 at 623.676 kb on - strand, within PGA1_c06090 at 623.724 kb on + strand, within PGA1_c06090 at 623.798 kb on + strand at 623.808 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Phaeo_ML1_MIT diversity barseq remove 621,265 - PGA1_c06060 0.83 -1.5 621,294 - PGA1_c06060 0.88 +2.5 621,494 + PGA1_c06070 0.14 +0.5 621,534 - PGA1_c06070 0.19 +0.5 621,672 - PGA1_c06070 0.35 +1.7 621,674 + PGA1_c06070 0.36 -0.0 621,674 + PGA1_c06070 0.36 +0.8 621,682 - PGA1_c06070 0.37 +0.4 621,725 - PGA1_c06070 0.42 -0.6 621,737 + PGA1_c06070 0.43 -0.0 621,745 - PGA1_c06070 0.44 +0.8 621,745 - PGA1_c06070 0.44 -0.5 621,755 - PGA1_c06070 0.45 -0.2 621,831 - PGA1_c06070 0.54 +1.0 621,844 + PGA1_c06070 0.56 +0.5 621,852 - PGA1_c06070 0.57 -0.9 621,961 - PGA1_c06070 0.69 -0.4 621,966 - PGA1_c06070 0.70 -1.0 621,997 + PGA1_c06070 0.74 +0.5 622,004 + PGA1_c06070 0.74 -0.8 622,005 - PGA1_c06070 0.75 +0.1 622,040 + PGA1_c06070 0.79 -1.0 622,087 - PGA1_c06070 0.84 -1.8 622,155 - -0.2 622,172 + -0.3 622,230 + -0.3 622,235 + -1.0 622,245 + +1.2 622,253 - -0.1 622,253 - -0.6 622,355 - PGA1_c06080 0.16 +0.5 622,467 + PGA1_c06080 0.34 +0.3 622,505 + PGA1_c06080 0.40 -0.5 622,535 + PGA1_c06080 0.45 -0.3 622,553 - PGA1_c06080 0.48 -1.2 622,579 - PGA1_c06080 0.53 -1.8 622,601 - PGA1_c06080 0.56 -1.0 622,626 + PGA1_c06080 0.61 +0.5 622,635 - PGA1_c06080 0.62 +1.9 622,721 - PGA1_c06080 0.76 +4.1 622,721 - PGA1_c06080 0.76 -1.0 622,737 - PGA1_c06080 0.79 -0.4 622,844 + -1.2 622,895 - +0.3 622,900 - +1.5 622,905 - -0.3 622,918 - -0.1 622,941 + -0.6 622,949 - -0.0 623,007 + PGA1_c06090 0.14 -1.3 623,033 + PGA1_c06090 0.17 -0.8 623,033 + PGA1_c06090 0.17 -1.5 623,033 + PGA1_c06090 0.17 +0.1 623,041 - PGA1_c06090 0.18 -1.3 623,041 - PGA1_c06090 0.18 +0.1 623,041 - PGA1_c06090 0.18 +2.4 623,041 - PGA1_c06090 0.18 -2.0 623,041 - PGA1_c06090 0.18 +0.3 623,128 - PGA1_c06090 0.27 -1.0 623,153 + PGA1_c06090 0.29 -0.9 623,222 - PGA1_c06090 0.36 -1.0 623,244 - PGA1_c06090 0.38 -0.5 623,256 + PGA1_c06090 0.40 -0.7 623,292 - PGA1_c06090 0.43 -0.3 623,374 + PGA1_c06090 0.52 -1.8 623,381 - PGA1_c06090 0.52 +0.2 623,425 + PGA1_c06090 0.57 -0.0 623,456 + PGA1_c06090 0.60 +0.2 623,464 - PGA1_c06090 0.61 -0.3 623,466 + PGA1_c06090 0.61 -1.2 623,474 - PGA1_c06090 0.62 -0.3 623,487 + PGA1_c06090 0.63 -0.4 623,502 + PGA1_c06090 0.65 -1.3 623,567 + PGA1_c06090 0.71 +1.1 623,575 - PGA1_c06090 0.72 +2.9 623,577 + PGA1_c06090 0.72 -1.3 623,577 + PGA1_c06090 0.72 -1.8 623,640 - PGA1_c06090 0.79 +1.5 623,668 + PGA1_c06090 0.82 +0.0 623,671 - PGA1_c06090 0.82 -0.0 623,676 - PGA1_c06090 0.82 +0.1 623,724 + PGA1_c06090 0.87 +0.7 623,798 + -0.7 623,808 - +0.9
Or see this region's nucleotide sequence