Experiment: Phaeo_ML1_MIT diversity barseq
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_78p00540 and PGA1_78p00550 are separated by 118 nucleotides PGA1_78p00550 and PGA1_78p00560 overlap by 4 nucleotides PGA1_78p00560 and PGA1_78p00570 are separated by 60 nucleotides
PGA1_78p00540: PGA1_78p00540 - hypothetical protein, at 68,689 to 69,441
_78p00540
PGA1_78p00550: PGA1_78p00550 - putative transcriptional regulator, ArsR family, at 69,560 to 69,922
_78p00550
PGA1_78p00560: PGA1_78p00560 - Uncharacterized conserved protein, at 69,919 to 70,332
_78p00560
PGA1_78p00570: PGA1_78p00570 - hypothetical protein, at 70,393 to 71,322
_78p00570
Position (kb)
69
70
71 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 68.929 kb on - strand, within PGA1_78p00540 at 69.046 kb on + strand, within PGA1_78p00540 at 69.111 kb on - strand, within PGA1_78p00540 at 69.213 kb on + strand, within PGA1_78p00540 at 69.969 kb on - strand, within PGA1_78p00560 at 69.977 kb on + strand, within PGA1_78p00560 at 69.980 kb on - strand, within PGA1_78p00560 at 69.985 kb on - strand, within PGA1_78p00560 at 70.023 kb on + strand, within PGA1_78p00560 at 70.113 kb on + strand, within PGA1_78p00560 at 70.539 kb on + strand, within PGA1_78p00570 at 70.796 kb on - strand, within PGA1_78p00570
Per-strain Table
Position Strand Gene LocusTag Fraction Phaeo_ML1_MIT diversity barseq remove 68,929 - PGA1_78p00540 0.32 +2.6 69,046 + PGA1_78p00540 0.47 +1.3 69,111 - PGA1_78p00540 0.56 +0.6 69,213 + PGA1_78p00540 0.70 +0.3 69,969 - PGA1_78p00560 0.12 +0.7 69,977 + PGA1_78p00560 0.14 -2.3 69,980 - PGA1_78p00560 0.15 -0.9 69,985 - PGA1_78p00560 0.16 +1.3 70,023 + PGA1_78p00560 0.25 -0.8 70,113 + PGA1_78p00560 0.47 -1.1 70,539 + PGA1_78p00570 0.16 +0.9 70,796 - PGA1_78p00570 0.43 -2.1
Or see this region's nucleotide sequence