Experiment: Phaeo_ML1_MIT diversity barseq
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_262p01410 and PGA1_262p01420 are separated by 631 nucleotides PGA1_262p01420 and PGA1_262p01430 overlap by 4 nucleotides
PGA1_262p01410: PGA1_262p01410 - malate/L-lactate dehydrogenase-like protein, at 152,967 to 153,404
_262p01410
PGA1_262p01420: PGA1_262p01420 - Protein of unknown function (DUF3726)., at 154,036 to 154,686
_262p01420
PGA1_262p01430: PGA1_262p01430 - hypothetical protein, at 154,683 to 156,374
_262p01430
Position (kb) 154
155 Strain fitness (log2 ratio) -2
-1
0
1
2 at 153.193 kb on + strand, within PGA1_262p01410 at 153.201 kb on - strand, within PGA1_262p01410 at 153.342 kb on - strand, within PGA1_262p01410 at 153.367 kb on - strand at 153.369 kb on - strand at 153.648 kb on - strand at 153.691 kb on - strand at 153.749 kb on - strand at 153.749 kb on - strand at 153.949 kb on + strand at 154.006 kb on - strand at 154.110 kb on + strand, within PGA1_262p01420 at 154.469 kb on + strand, within PGA1_262p01420 at 154.697 kb on + strand at 154.743 kb on - strand at 154.839 kb on + strand at 154.995 kb on + strand, within PGA1_262p01430 at 155.002 kb on + strand, within PGA1_262p01430 at 155.171 kb on + strand, within PGA1_262p01430 at 155.174 kb on - strand, within PGA1_262p01430 at 155.174 kb on - strand, within PGA1_262p01430 at 155.261 kb on - strand, within PGA1_262p01430 at 155.316 kb on - strand, within PGA1_262p01430 at 155.350 kb on + strand, within PGA1_262p01430 at 155.369 kb on + strand, within PGA1_262p01430 at 155.377 kb on - strand, within PGA1_262p01430 at 155.479 kb on - strand, within PGA1_262p01430 at 155.509 kb on + strand, within PGA1_262p01430 at 155.517 kb on - strand, within PGA1_262p01430 at 155.527 kb on - strand, within PGA1_262p01430 at 155.678 kb on - strand, within PGA1_262p01430
Per-strain Table
Position Strand Gene LocusTag Fraction Phaeo_ML1_MIT diversity barseq remove 153,193 + PGA1_262p01410 0.52 -0.2 153,201 - PGA1_262p01410 0.53 -0.3 153,342 - PGA1_262p01410 0.86 -0.5 153,367 - +0.6 153,369 - -0.3 153,648 - +1.0 153,691 - -0.8 153,749 - +0.2 153,749 - +2.2 153,949 + +0.6 154,006 - -0.0 154,110 + PGA1_262p01420 0.11 -0.4 154,469 + PGA1_262p01420 0.67 -0.2 154,697 + -0.2 154,743 - -0.1 154,839 + -2.2 154,995 + PGA1_262p01430 0.18 +2.2 155,002 + PGA1_262p01430 0.19 -0.4 155,171 + PGA1_262p01430 0.29 +1.0 155,174 - PGA1_262p01430 0.29 +2.2 155,174 - PGA1_262p01430 0.29 -0.5 155,261 - PGA1_262p01430 0.34 +0.3 155,316 - PGA1_262p01430 0.37 +2.2 155,350 + PGA1_262p01430 0.39 +2.2 155,369 + PGA1_262p01430 0.41 -0.7 155,377 - PGA1_262p01430 0.41 +1.6 155,479 - PGA1_262p01430 0.47 -0.4 155,509 + PGA1_262p01430 0.49 +0.1 155,517 - PGA1_262p01430 0.49 +1.2 155,527 - PGA1_262p01430 0.50 -1.9 155,678 - PGA1_262p01430 0.59 +0.0
Or see this region's nucleotide sequence