Experiment: Phaeo_ML1_MIT diversity barseq
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_262p00260 and PGA1_262p00270 are separated by 155 nucleotides PGA1_262p00270 and PGA1_262p00280 are separated by 93 nucleotides PGA1_262p00280 and PGA1_262p00290 are separated by 200 nucleotides PGA1_262p00290 and PGA1_262p00300 are separated by 148 nucleotides
PGA1_262p00260: PGA1_262p00260 - hypothetical protein, at 30,070 to 30,510
_262p00260
PGA1_262p00270: PGA1_262p00270 - transcriptional regulator, GntR family, at 30,666 to 31,334
_262p00270
PGA1_262p00280: PGA1_262p00280 - putative proline racemase, at 31,428 to 32,453
_262p00280
PGA1_262p00290: PGA1_262p00290 - putative transcriptional regulator, at 32,654 to 33,265
_262p00290
PGA1_262p00300: PGA1_262p00300 - ABC transporter, extracellular solute-binding protein, at 33,414 to 35,000
_262p00300
Position (kb) 31
32
33 Strain fitness (log2 ratio) -2
-1
0
1
2 at 30.493 kb on + strand at 30.589 kb on + strand at 30.597 kb on - strand at 30.597 kb on - strand at 30.657 kb on + strand at 30.703 kb on + strand at 30.822 kb on + strand, within PGA1_262p00270 at 30.989 kb on + strand, within PGA1_262p00270 at 31.150 kb on - strand, within PGA1_262p00270 at 31.175 kb on - strand, within PGA1_262p00270 at 31.200 kb on + strand, within PGA1_262p00270 at 31.248 kb on - strand, within PGA1_262p00270 at 31.340 kb on + strand at 31.388 kb on + strand at 31.393 kb on + strand at 31.491 kb on + strand at 31.620 kb on + strand, within PGA1_262p00280 at 31.620 kb on + strand, within PGA1_262p00280 at 31.643 kb on - strand, within PGA1_262p00280 at 31.712 kb on + strand, within PGA1_262p00280 at 31.755 kb on - strand, within PGA1_262p00280 at 31.808 kb on - strand, within PGA1_262p00280 at 31.896 kb on + strand, within PGA1_262p00280 at 31.918 kb on + strand, within PGA1_262p00280 at 31.926 kb on - strand, within PGA1_262p00280 at 32.259 kb on - strand, within PGA1_262p00280 at 32.262 kb on + strand, within PGA1_262p00280 at 32.316 kb on + strand, within PGA1_262p00280 at 32.316 kb on + strand, within PGA1_262p00280 at 32.325 kb on - strand, within PGA1_262p00280 at 32.355 kb on - strand at 32.396 kb on + strand at 32.460 kb on + strand at 32.613 kb on - strand at 32.623 kb on - strand at 32.623 kb on - strand at 32.623 kb on - strand at 32.667 kb on - strand at 32.712 kb on + strand at 32.712 kb on + strand at 32.720 kb on + strand, within PGA1_262p00290 at 32.720 kb on - strand, within PGA1_262p00290 at 32.720 kb on - strand, within PGA1_262p00290 at 32.780 kb on + strand, within PGA1_262p00290 at 33.016 kb on + strand, within PGA1_262p00290 at 33.016 kb on + strand, within PGA1_262p00290 at 33.016 kb on + strand, within PGA1_262p00290 at 33.016 kb on + strand, within PGA1_262p00290 at 33.049 kb on - strand, within PGA1_262p00290 at 33.122 kb on - strand, within PGA1_262p00290 at 33.151 kb on + strand, within PGA1_262p00290 at 33.159 kb on - strand, within PGA1_262p00290 at 33.159 kb on - strand, within PGA1_262p00290 at 33.172 kb on - strand, within PGA1_262p00290 at 33.174 kb on + strand, within PGA1_262p00290 at 33.174 kb on + strand, within PGA1_262p00290 at 33.237 kb on - strand at 33.259 kb on + strand at 33.278 kb on + strand at 33.286 kb on - strand at 33.305 kb on + strand at 33.399 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Phaeo_ML1_MIT diversity barseq remove 30,493 + +0.8 30,589 + -1.0 30,597 - +0.2 30,597 - +0.0 30,657 + +0.5 30,703 + -0.7 30,822 + PGA1_262p00270 0.23 +0.5 30,989 + PGA1_262p00270 0.48 +1.4 31,150 - PGA1_262p00270 0.72 -0.8 31,175 - PGA1_262p00270 0.76 -0.5 31,200 + PGA1_262p00270 0.80 -0.5 31,248 - PGA1_262p00270 0.87 -0.1 31,340 + -0.4 31,388 + +0.2 31,393 + -0.3 31,491 + -1.0 31,620 + PGA1_262p00280 0.19 -0.3 31,620 + PGA1_262p00280 0.19 -2.4 31,643 - PGA1_262p00280 0.21 -0.5 31,712 + PGA1_262p00280 0.28 -1.5 31,755 - PGA1_262p00280 0.32 +0.2 31,808 - PGA1_262p00280 0.37 -0.7 31,896 + PGA1_262p00280 0.46 +0.9 31,918 + PGA1_262p00280 0.48 -0.7 31,926 - PGA1_262p00280 0.49 +1.2 32,259 - PGA1_262p00280 0.81 -1.1 32,262 + PGA1_262p00280 0.81 -0.4 32,316 + PGA1_262p00280 0.87 -0.6 32,316 + PGA1_262p00280 0.87 +0.1 32,325 - PGA1_262p00280 0.87 -0.6 32,355 - +0.1 32,396 + +0.4 32,460 + -1.2 32,613 - -1.8 32,623 - +0.6 32,623 - -0.8 32,623 - -0.3 32,667 - +1.4 32,712 + +2.0 32,712 + +0.4 32,720 + PGA1_262p00290 0.11 +0.3 32,720 - PGA1_262p00290 0.11 +0.1 32,720 - PGA1_262p00290 0.11 +1.2 32,780 + PGA1_262p00290 0.21 +1.3 33,016 + PGA1_262p00290 0.59 +0.3 33,016 + PGA1_262p00290 0.59 +0.6 33,016 + PGA1_262p00290 0.59 +1.2 33,016 + PGA1_262p00290 0.59 +0.4 33,049 - PGA1_262p00290 0.65 +0.7 33,122 - PGA1_262p00290 0.76 +1.4 33,151 + PGA1_262p00290 0.81 +0.1 33,159 - PGA1_262p00290 0.83 -0.7 33,159 - PGA1_262p00290 0.83 +0.4 33,172 - PGA1_262p00290 0.85 +0.5 33,174 + PGA1_262p00290 0.85 +0.7 33,174 + PGA1_262p00290 0.85 -1.2 33,237 - +0.0 33,259 + -0.7 33,278 + -0.3 33,286 - -0.5 33,305 + +0.2 33,399 + +0.4
Or see this region's nucleotide sequence