Experiment: Phaeo_ML1_MIT diversity barseq
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c03180 and PGA1_c03190 are separated by 88 nucleotides PGA1_c03190 and PGA1_c03200 are separated by 8 nucleotides PGA1_c03200 and PGA1_c03210 are separated by 79 nucleotides PGA1_c03210 and PGA1_c03220 overlap by 4 nucleotides PGA1_c03220 and PGA1_c03230 are separated by 59 nucleotides
PGA1_c03180: PGA1_c03180 - hypothetical protein, at 306,909 to 307,394
_c03180
PGA1_c03190: PGA1_c03190 - Bacterial aa3 type cytochrome c oxidase subunit IV., at 307,483 to 307,617
_c03190
PGA1_c03200: PGA1_c03200 - hypothetical protein, at 307,626 to 308,294
_c03200
PGA1_c03210: PGA1_c03210 - branched-chain amino acid transport protein azlD-like protein, at 308,374 to 308,709
_c03210
PGA1_c03220: PGA1_c03220 - branched-chain amino acid transport protein azlC-like protein, at 308,706 to 309,431
_c03220
PGA1_c03230: PGA1_c03230 - putative formate dehydrogenase, subunit FdhD, at 309,491 to 310,393
_c03230
Position (kb) 308
309 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 307.423 kb on + strand at 307.423 kb on + strand at 307.423 kb on + strand at 307.431 kb on - strand at 307.571 kb on - strand, within PGA1_c03190 at 307.619 kb on - strand at 307.700 kb on - strand, within PGA1_c03200 at 307.700 kb on - strand, within PGA1_c03200 at 307.750 kb on - strand, within PGA1_c03200 at 307.843 kb on - strand, within PGA1_c03200 at 307.870 kb on + strand, within PGA1_c03200 at 307.981 kb on + strand, within PGA1_c03200 at 307.989 kb on - strand, within PGA1_c03200 at 308.020 kb on + strand, within PGA1_c03200 at 308.035 kb on - strand, within PGA1_c03200 at 308.054 kb on - strand, within PGA1_c03200 at 308.144 kb on - strand, within PGA1_c03200 at 308.194 kb on - strand, within PGA1_c03200 at 308.198 kb on + strand, within PGA1_c03200 at 308.234 kb on - strand at 308.264 kb on + strand at 308.272 kb on - strand at 308.272 kb on - strand at 308.306 kb on - strand at 308.367 kb on + strand at 308.375 kb on - strand at 308.405 kb on - strand at 308.436 kb on - strand, within PGA1_c03210 at 308.524 kb on + strand, within PGA1_c03210 at 308.527 kb on + strand, within PGA1_c03210 at 308.567 kb on + strand, within PGA1_c03210 at 308.567 kb on - strand, within PGA1_c03210 at 308.575 kb on - strand, within PGA1_c03210 at 308.595 kb on + strand, within PGA1_c03210 at 308.648 kb on - strand, within PGA1_c03210 at 308.675 kb on + strand, within PGA1_c03210 at 308.675 kb on + strand, within PGA1_c03210 at 308.675 kb on + strand, within PGA1_c03210 at 308.675 kb on + strand, within PGA1_c03210 at 308.675 kb on + strand, within PGA1_c03210 at 308.675 kb on + strand, within PGA1_c03210 at 308.683 kb on - strand at 308.683 kb on - strand at 308.683 kb on - strand at 308.683 kb on - strand at 308.683 kb on - strand at 308.686 kb on + strand at 308.693 kb on - strand at 308.693 kb on - strand at 308.718 kb on - strand at 308.718 kb on - strand at 308.754 kb on - strand at 308.756 kb on + strand at 308.761 kb on - strand at 308.789 kb on - strand, within PGA1_c03220 at 308.803 kb on - strand at 308.816 kb on - strand, within PGA1_c03220 at 308.826 kb on - strand, within PGA1_c03220 at 308.826 kb on - strand, within PGA1_c03220 at 308.826 kb on - strand, within PGA1_c03220 at 308.826 kb on - strand, within PGA1_c03220 at 308.826 kb on - strand, within PGA1_c03220 at 308.868 kb on - strand, within PGA1_c03220 at 308.879 kb on + strand, within PGA1_c03220 at 308.879 kb on + strand, within PGA1_c03220 at 308.879 kb on + strand, within PGA1_c03220 at 308.887 kb on - strand, within PGA1_c03220 at 308.957 kb on + strand, within PGA1_c03220 at 308.975 kb on - strand, within PGA1_c03220 at 308.981 kb on - strand, within PGA1_c03220 at 309.005 kb on + strand, within PGA1_c03220 at 309.035 kb on + strand, within PGA1_c03220 at 309.035 kb on + strand, within PGA1_c03220 at 309.035 kb on + strand, within PGA1_c03220 at 309.043 kb on - strand, within PGA1_c03220 at 309.068 kb on + strand, within PGA1_c03220 at 309.068 kb on + strand, within PGA1_c03220 at 309.082 kb on + strand, within PGA1_c03220 at 309.090 kb on - strand, within PGA1_c03220 at 309.154 kb on - strand, within PGA1_c03220 at 309.250 kb on + strand, within PGA1_c03220 at 309.258 kb on - strand, within PGA1_c03220 at 309.258 kb on - strand, within PGA1_c03220 at 309.258 kb on - strand, within PGA1_c03220 at 309.268 kb on - strand, within PGA1_c03220 at 309.280 kb on - strand, within PGA1_c03220 at 309.304 kb on + strand, within PGA1_c03220 at 309.306 kb on - strand, within PGA1_c03220 at 309.459 kb on - strand at 309.460 kb on + strand at 309.460 kb on + strand at 309.468 kb on - strand at 309.468 kb on - strand at 309.468 kb on - strand at 309.468 kb on - strand at 309.515 kb on - strand at 309.520 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Phaeo_ML1_MIT diversity barseq remove 307,423 + +1.0 307,423 + +0.2 307,423 + -0.2 307,431 - +0.9 307,571 - PGA1_c03190 0.65 -0.2 307,619 - +0.3 307,700 - PGA1_c03200 0.11 -0.1 307,700 - PGA1_c03200 0.11 -0.7 307,750 - PGA1_c03200 0.19 -0.9 307,843 - PGA1_c03200 0.32 -0.7 307,870 + PGA1_c03200 0.36 +0.4 307,981 + PGA1_c03200 0.53 -0.3 307,989 - PGA1_c03200 0.54 +0.8 308,020 + PGA1_c03200 0.59 -0.3 308,035 - PGA1_c03200 0.61 -0.0 308,054 - PGA1_c03200 0.64 -0.3 308,144 - PGA1_c03200 0.77 +1.1 308,194 - PGA1_c03200 0.85 -0.5 308,198 + PGA1_c03200 0.86 -0.3 308,234 - -2.6 308,264 + -1.6 308,272 - -0.8 308,272 - -1.3 308,306 - +1.8 308,367 + -0.3 308,375 - -0.6 308,405 - +1.2 308,436 - PGA1_c03210 0.18 -0.5 308,524 + PGA1_c03210 0.45 +1.5 308,527 + PGA1_c03210 0.46 -0.4 308,567 + PGA1_c03210 0.57 +0.2 308,567 - PGA1_c03210 0.57 +0.5 308,575 - PGA1_c03210 0.60 -0.5 308,595 + PGA1_c03210 0.66 +0.3 308,648 - PGA1_c03210 0.82 -1.9 308,675 + PGA1_c03210 0.90 -0.5 308,675 + PGA1_c03210 0.90 -0.3 308,675 + PGA1_c03210 0.90 -0.7 308,675 + PGA1_c03210 0.90 -0.3 308,675 + PGA1_c03210 0.90 -1.3 308,675 + PGA1_c03210 0.90 -0.5 308,683 - +0.3 308,683 - +0.2 308,683 - -1.2 308,683 - -0.1 308,683 - +3.5 308,686 + +0.5 308,693 - -0.5 308,693 - +1.0 308,718 - -0.0 308,718 - -0.2 308,754 - -0.9 308,756 + -0.8 308,761 - -1.0 308,789 - PGA1_c03220 0.11 -0.1 308,803 - +0.5 308,816 - PGA1_c03220 0.15 -0.6 308,826 - PGA1_c03220 0.17 +0.1 308,826 - PGA1_c03220 0.17 -1.1 308,826 - PGA1_c03220 0.17 -0.3 308,826 - PGA1_c03220 0.17 -0.4 308,826 - PGA1_c03220 0.17 +0.2 308,868 - PGA1_c03220 0.22 +0.6 308,879 + PGA1_c03220 0.24 -0.2 308,879 + PGA1_c03220 0.24 +0.1 308,879 + PGA1_c03220 0.24 -0.4 308,887 - PGA1_c03220 0.25 -0.4 308,957 + PGA1_c03220 0.35 -0.4 308,975 - PGA1_c03220 0.37 +1.3 308,981 - PGA1_c03220 0.38 -0.0 309,005 + PGA1_c03220 0.41 +0.5 309,035 + PGA1_c03220 0.45 -0.1 309,035 + PGA1_c03220 0.45 -0.1 309,035 + PGA1_c03220 0.45 -0.6 309,043 - PGA1_c03220 0.46 -0.9 309,068 + PGA1_c03220 0.50 +0.5 309,068 + PGA1_c03220 0.50 -0.1 309,082 + PGA1_c03220 0.52 -0.2 309,090 - PGA1_c03220 0.53 +0.5 309,154 - PGA1_c03220 0.62 -1.0 309,250 + PGA1_c03220 0.75 -0.9 309,258 - PGA1_c03220 0.76 -0.0 309,258 - PGA1_c03220 0.76 +0.3 309,258 - PGA1_c03220 0.76 +0.2 309,268 - PGA1_c03220 0.77 +2.1 309,280 - PGA1_c03220 0.79 -1.0 309,304 + PGA1_c03220 0.82 -0.1 309,306 - PGA1_c03220 0.83 +0.2 309,459 - -2.5 309,460 + -1.6 309,460 + +0.8 309,468 - +0.3 309,468 - +1.7 309,468 - -1.5 309,468 - +0.1 309,515 - +0.2 309,520 - +0.0
Or see this region's nucleotide sequence