Strain Fitness in Phaeobacter inhibens DSM 17395 around PGA1_c00030

Experiment: Phaeo_ML1_MIT diversity barseq

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPGA1_c00020 and PGA1_c00030 are separated by 97 nucleotidesPGA1_c00030 and PGA1_c00040 overlap by 4 nucleotidesPGA1_c00040 and PGA1_c00050 are separated by 14 nucleotides PGA1_c00020: PGA1_c00020 - DNA polymerase III subunit beta, at 1,838 to 2,959 _c00020 PGA1_c00030: PGA1_c00030 - DNA replication and repair protein RecF, at 3,057 to 4,154 _c00030 PGA1_c00040: PGA1_c00040 - putative LysE type translocator, at 4,151 to 4,771 _c00040 PGA1_c00050: PGA1_c00050 - putative transcriptional regulator, tetR family, at 4,786 to 5,385 _c00050 Position (kb) 3 4 5Strain fitness (log2 ratio) -2 -1 0 1 2 3at 2.953 kb on + strandat 2.953 kb on + strandat 2.953 kb on + strandat 2.961 kb on - strandat 2.963 kb on + strandat 2.969 kb on + strandat 2.990 kb on + strandat 2.990 kb on + strandat 2.998 kb on - strandat 2.998 kb on - strandat 3.053 kb on + strandat 3.070 kb on - strandat 3.073 kb on + strandat 3.073 kb on + strandat 3.093 kb on + strandat 3.131 kb on + strandat 3.248 kb on + strand, within PGA1_c00030at 3.281 kb on + strand, within PGA1_c00030at 3.289 kb on + strand, within PGA1_c00030at 3.317 kb on - strand, within PGA1_c00030at 3.342 kb on - strand, within PGA1_c00030at 3.386 kb on - strand, within PGA1_c00030at 3.510 kb on + strand, within PGA1_c00030at 3.584 kb on - strand, within PGA1_c00030at 3.640 kb on + strand, within PGA1_c00030at 3.674 kb on - strand, within PGA1_c00030at 3.707 kb on - strand, within PGA1_c00030at 3.732 kb on + strand, within PGA1_c00030at 3.750 kb on - strand, within PGA1_c00030at 3.763 kb on + strand, within PGA1_c00030at 3.799 kb on + strand, within PGA1_c00030at 3.807 kb on - strand, within PGA1_c00030at 3.807 kb on - strand, within PGA1_c00030at 3.831 kb on - strand, within PGA1_c00030at 3.848 kb on + strand, within PGA1_c00030at 3.848 kb on + strand, within PGA1_c00030at 3.897 kb on + strand, within PGA1_c00030at 4.009 kb on - strand, within PGA1_c00030at 4.035 kb on - strand, within PGA1_c00030at 4.046 kb on + strandat 4.066 kb on + strandat 4.066 kb on + strandat 4.066 kb on + strandat 4.066 kb on + strandat 4.074 kb on - strandat 4.074 kb on - strandat 4.074 kb on - strandat 4.098 kb on + strandat 4.103 kb on + strandat 4.111 kb on + strandat 4.114 kb on - strandat 4.132 kb on + strandat 4.195 kb on - strandat 4.212 kb on - strandat 4.235 kb on - strand, within PGA1_c00040at 4.290 kb on - strand, within PGA1_c00040at 4.369 kb on + strand, within PGA1_c00040at 4.402 kb on - strand, within PGA1_c00040at 4.480 kb on + strand, within PGA1_c00040at 4.522 kb on - strand, within PGA1_c00040at 4.580 kb on + strand, within PGA1_c00040at 4.580 kb on + strand, within PGA1_c00040at 4.581 kb on - strand, within PGA1_c00040at 4.656 kb on - strand, within PGA1_c00040at 4.656 kb on - strand, within PGA1_c00040at 4.666 kb on - strand, within PGA1_c00040at 4.671 kb on - strand, within PGA1_c00040at 4.777 kb on + strandat 4.780 kb on - strandat 4.780 kb on - strandat 4.785 kb on + strandat 4.825 kb on + strandat 4.858 kb on + strand, within PGA1_c00050at 4.873 kb on + strand, within PGA1_c00050at 4.913 kb on + strand, within PGA1_c00050at 4.921 kb on - strand, within PGA1_c00050at 4.921 kb on - strand, within PGA1_c00050at 4.950 kb on + strand, within PGA1_c00050at 4.971 kb on + strand, within PGA1_c00050at 4.979 kb on - strand, within PGA1_c00050at 5.031 kb on - strand, within PGA1_c00050at 5.057 kb on - strand, within PGA1_c00050at 5.068 kb on - strand, within PGA1_c00050at 5.103 kb on + strand, within PGA1_c00050at 5.148 kb on - strand, within PGA1_c00050at 5.152 kb on + strand, within PGA1_c00050

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Per-strain Table

Position Strand Gene LocusTag Fraction Phaeo_ML1_MIT diversity barseq
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2,953 + +0.2
2,953 + -0.0
2,953 + -0.3
2,961 - -0.8
2,963 + -0.0
2,969 + +0.1
2,990 + -1.0
2,990 + +0.7
2,998 - -0.2
2,998 - -0.5
3,053 + -1.3
3,070 - -0.5
3,073 + +2.1
3,073 + -0.9
3,093 + +0.2
3,131 + +0.0
3,248 + PGA1_c00030 0.17 +1.1
3,281 + PGA1_c00030 0.20 -1.5
3,289 + PGA1_c00030 0.21 -2.5
3,317 - PGA1_c00030 0.24 +1.8
3,342 - PGA1_c00030 0.26 -1.3
3,386 - PGA1_c00030 0.30 -0.5
3,510 + PGA1_c00030 0.41 -0.8
3,584 - PGA1_c00030 0.48 +0.9
3,640 + PGA1_c00030 0.53 -0.3
3,674 - PGA1_c00030 0.56 +1.5
3,707 - PGA1_c00030 0.59 -0.4
3,732 + PGA1_c00030 0.61 -0.8
3,750 - PGA1_c00030 0.63 -0.5
3,763 + PGA1_c00030 0.64 +0.8
3,799 + PGA1_c00030 0.68 +0.5
3,807 - PGA1_c00030 0.68 +0.5
3,807 - PGA1_c00030 0.68 +1.1
3,831 - PGA1_c00030 0.70 -0.5
3,848 + PGA1_c00030 0.72 -0.5
3,848 + PGA1_c00030 0.72 +0.3
3,897 + PGA1_c00030 0.77 +3.1
4,009 - PGA1_c00030 0.87 +0.5
4,035 - PGA1_c00030 0.89 +1.1
4,046 + -0.0
4,066 + +1.2
4,066 + +0.8
4,066 + -0.3
4,066 + +0.1
4,074 - +2.1
4,074 - +0.2
4,074 - -0.5
4,098 + -0.9
4,103 + +0.2
4,111 + +0.7
4,114 - -0.5
4,132 + +0.8
4,195 - -0.4
4,212 - +0.2
4,235 - PGA1_c00040 0.14 -0.1
4,290 - PGA1_c00040 0.22 -0.0
4,369 + PGA1_c00040 0.35 +0.1
4,402 - PGA1_c00040 0.40 +1.2
4,480 + PGA1_c00040 0.53 +1.1
4,522 - PGA1_c00040 0.60 +0.1
4,580 + PGA1_c00040 0.69 +1.9
4,580 + PGA1_c00040 0.69 -0.5
4,581 - PGA1_c00040 0.69 +0.1
4,656 - PGA1_c00040 0.81 -0.9
4,656 - PGA1_c00040 0.81 +0.0
4,666 - PGA1_c00040 0.83 +1.5
4,671 - PGA1_c00040 0.84 -1.2
4,777 + -0.6
4,780 - -1.0
4,780 - +0.3
4,785 + +0.1
4,825 + +0.5
4,858 + PGA1_c00050 0.12 +0.5
4,873 + PGA1_c00050 0.14 +0.5
4,913 + PGA1_c00050 0.21 +0.5
4,921 - PGA1_c00050 0.23 +0.5
4,921 - PGA1_c00050 0.23 +2.1
4,950 + PGA1_c00050 0.27 +0.5
4,971 + PGA1_c00050 0.31 -1.0
4,979 - PGA1_c00050 0.32 +0.5
5,031 - PGA1_c00050 0.41 +2.1
5,057 - PGA1_c00050 0.45 +1.5
5,068 - PGA1_c00050 0.47 +2.1
5,103 + PGA1_c00050 0.53 +2.1
5,148 - PGA1_c00050 0.60 +0.5
5,152 + PGA1_c00050 0.61 +0.5

Or see this region's nucleotide sequence