Experiment: Phaeo_ML1_MIT diversity barseq
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c29670 and PGA1_c29680 are separated by 133 nucleotides PGA1_c29680 and PGA1_c29690 overlap by 4 nucleotides PGA1_c29690 and PGA1_c29700 are separated by 130 nucleotides
PGA1_c29670: PGA1_c29670 - small heat shock protein IbpA, at 3,104,120 to 3,104,578
_c29670
PGA1_c29680: PGA1_c29680 - serine protease-like protein, at 3,104,712 to 3,105,527
_c29680
PGA1_c29690: PGA1_c29690 - serine protease-like protein, at 3,105,524 to 3,106,378
_c29690
PGA1_c29700: PGA1_c29700 - D-lactate dehydrogenase, iron-sulfur subunit GlcF (EC 1.1.99.6) (from data) , at 3,106,509 to 3,107,843
_c29700
Position (kb)
3105
3106
3107 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3104.549 kb on + strand at 3104.557 kb on - strand at 3104.639 kb on - strand at 3104.641 kb on + strand at 3104.641 kb on + strand at 3104.692 kb on - strand at 3104.839 kb on - strand, within PGA1_c29680 at 3104.870 kb on + strand, within PGA1_c29680 at 3104.878 kb on - strand, within PGA1_c29680 at 3104.880 kb on + strand, within PGA1_c29680 at 3104.880 kb on + strand, within PGA1_c29680 at 3104.880 kb on + strand, within PGA1_c29680 at 3104.883 kb on - strand, within PGA1_c29680 at 3104.888 kb on - strand, within PGA1_c29680 at 3104.888 kb on - strand, within PGA1_c29680 at 3104.888 kb on - strand, within PGA1_c29680 at 3104.901 kb on - strand, within PGA1_c29680 at 3104.993 kb on - strand, within PGA1_c29680 at 3105.038 kb on + strand, within PGA1_c29680 at 3105.131 kb on + strand, within PGA1_c29680 at 3105.153 kb on - strand, within PGA1_c29680 at 3105.223 kb on - strand, within PGA1_c29680 at 3105.232 kb on + strand, within PGA1_c29680 at 3105.233 kb on - strand, within PGA1_c29680 at 3105.261 kb on + strand, within PGA1_c29680 at 3105.269 kb on - strand, within PGA1_c29680 at 3105.274 kb on - strand, within PGA1_c29680 at 3105.287 kb on + strand, within PGA1_c29680 at 3105.292 kb on + strand, within PGA1_c29680 at 3105.304 kb on + strand, within PGA1_c29680 at 3105.400 kb on - strand, within PGA1_c29680 at 3105.417 kb on + strand, within PGA1_c29680 at 3105.419 kb on + strand, within PGA1_c29680 at 3105.461 kb on + strand at 3105.478 kb on + strand at 3105.502 kb on + strand at 3105.506 kb on + strand at 3105.506 kb on + strand at 3105.510 kb on - strand at 3105.514 kb on - strand at 3105.538 kb on + strand at 3105.618 kb on + strand, within PGA1_c29690 at 3105.720 kb on + strand, within PGA1_c29690 at 3105.736 kb on + strand, within PGA1_c29690 at 3105.767 kb on + strand, within PGA1_c29690 at 3105.780 kb on + strand, within PGA1_c29690 at 3105.788 kb on - strand, within PGA1_c29690 at 3105.833 kb on - strand, within PGA1_c29690 at 3105.854 kb on - strand, within PGA1_c29690 at 3105.857 kb on + strand, within PGA1_c29690 at 3105.862 kb on + strand, within PGA1_c29690 at 3105.980 kb on + strand, within PGA1_c29690 at 3105.998 kb on - strand, within PGA1_c29690 at 3106.054 kb on - strand, within PGA1_c29690 at 3106.102 kb on - strand, within PGA1_c29690 at 3106.119 kb on - strand, within PGA1_c29690 at 3106.157 kb on - strand, within PGA1_c29690 at 3106.204 kb on + strand, within PGA1_c29690 at 3106.241 kb on - strand, within PGA1_c29690 at 3106.248 kb on + strand, within PGA1_c29690 at 3106.248 kb on + strand, within PGA1_c29690 at 3106.302 kb on + strand at 3106.315 kb on - strand at 3106.322 kb on + strand at 3106.325 kb on - strand at 3106.330 kb on - strand at 3106.389 kb on + strand at 3106.392 kb on - strand at 3106.418 kb on + strand at 3106.470 kb on + strand at 3106.510 kb on + strand at 3106.510 kb on + strand at 3106.549 kb on - strand at 3106.559 kb on - strand at 3106.607 kb on + strand at 3106.625 kb on - strand at 3106.723 kb on + strand, within PGA1_c29700 at 3106.788 kb on - strand, within PGA1_c29700 at 3106.809 kb on - strand, within PGA1_c29700 at 3106.820 kb on - strand, within PGA1_c29700 at 3106.820 kb on - strand, within PGA1_c29700 at 3106.827 kb on + strand, within PGA1_c29700 at 3106.849 kb on + strand, within PGA1_c29700 at 3106.849 kb on + strand, within PGA1_c29700 at 3106.849 kb on + strand, within PGA1_c29700 at 3106.849 kb on + strand, within PGA1_c29700 at 3106.849 kb on + strand, within PGA1_c29700 at 3106.857 kb on - strand, within PGA1_c29700 at 3106.857 kb on - strand, within PGA1_c29700 at 3106.871 kb on + strand, within PGA1_c29700 at 3106.879 kb on - strand, within PGA1_c29700 at 3106.893 kb on + strand, within PGA1_c29700 at 3106.901 kb on - strand, within PGA1_c29700 at 3106.906 kb on + strand, within PGA1_c29700 at 3106.918 kb on + strand, within PGA1_c29700 at 3106.926 kb on - strand, within PGA1_c29700 at 3107.041 kb on - strand, within PGA1_c29700 at 3107.061 kb on - strand, within PGA1_c29700 at 3107.079 kb on - strand, within PGA1_c29700 at 3107.088 kb on + strand, within PGA1_c29700 at 3107.133 kb on + strand, within PGA1_c29700 at 3107.230 kb on - strand, within PGA1_c29700 at 3107.261 kb on - strand, within PGA1_c29700 at 3107.262 kb on + strand, within PGA1_c29700 at 3107.280 kb on + strand, within PGA1_c29700 at 3107.280 kb on + strand, within PGA1_c29700 at 3107.283 kb on - strand, within PGA1_c29700 at 3107.330 kb on + strand, within PGA1_c29700
Per-strain Table
Position Strand Gene LocusTag Fraction Phaeo_ML1_MIT diversity barseq remove 3,104,549 + +0.3 3,104,557 - +0.9 3,104,639 - +1.2 3,104,641 + +1.9 3,104,641 + -1.5 3,104,692 - +0.0 3,104,839 - PGA1_c29680 0.16 -0.2 3,104,870 + PGA1_c29680 0.19 -0.1 3,104,878 - PGA1_c29680 0.20 +0.1 3,104,880 + PGA1_c29680 0.21 +1.8 3,104,880 + PGA1_c29680 0.21 -0.1 3,104,880 + PGA1_c29680 0.21 +1.3 3,104,883 - PGA1_c29680 0.21 +0.5 3,104,888 - PGA1_c29680 0.22 +0.7 3,104,888 - PGA1_c29680 0.22 +1.5 3,104,888 - PGA1_c29680 0.22 -0.3 3,104,901 - PGA1_c29680 0.23 +0.0 3,104,993 - PGA1_c29680 0.34 -0.1 3,105,038 + PGA1_c29680 0.40 +0.2 3,105,131 + PGA1_c29680 0.51 +0.9 3,105,153 - PGA1_c29680 0.54 -1.2 3,105,223 - PGA1_c29680 0.63 -0.7 3,105,232 + PGA1_c29680 0.64 +0.3 3,105,233 - PGA1_c29680 0.64 +0.5 3,105,261 + PGA1_c29680 0.67 +0.9 3,105,269 - PGA1_c29680 0.68 -0.1 3,105,274 - PGA1_c29680 0.69 -0.6 3,105,287 + PGA1_c29680 0.70 +0.3 3,105,292 + PGA1_c29680 0.71 +0.0 3,105,304 + PGA1_c29680 0.73 +1.1 3,105,400 - PGA1_c29680 0.84 +1.2 3,105,417 + PGA1_c29680 0.86 -0.3 3,105,419 + PGA1_c29680 0.87 +0.5 3,105,461 + +0.6 3,105,478 + +1.4 3,105,502 + +0.3 3,105,506 + +0.0 3,105,506 + -0.8 3,105,510 - +1.1 3,105,514 - -0.2 3,105,538 + -0.5 3,105,618 + PGA1_c29690 0.11 -1.0 3,105,720 + PGA1_c29690 0.23 +1.1 3,105,736 + PGA1_c29690 0.25 -1.8 3,105,767 + PGA1_c29690 0.28 -0.7 3,105,780 + PGA1_c29690 0.30 -0.1 3,105,788 - PGA1_c29690 0.31 -0.5 3,105,833 - PGA1_c29690 0.36 -0.7 3,105,854 - PGA1_c29690 0.39 +1.5 3,105,857 + PGA1_c29690 0.39 -1.4 3,105,862 + PGA1_c29690 0.40 +2.9 3,105,980 + PGA1_c29690 0.53 -0.7 3,105,998 - PGA1_c29690 0.55 -0.2 3,106,054 - PGA1_c29690 0.62 -0.1 3,106,102 - PGA1_c29690 0.68 -0.4 3,106,119 - PGA1_c29690 0.70 -0.5 3,106,157 - PGA1_c29690 0.74 -1.4 3,106,204 + PGA1_c29690 0.80 -1.3 3,106,241 - PGA1_c29690 0.84 +2.7 3,106,248 + PGA1_c29690 0.85 +0.5 3,106,248 + PGA1_c29690 0.85 -0.7 3,106,302 + +0.1 3,106,315 - -1.5 3,106,322 + -1.5 3,106,325 - -0.1 3,106,330 - -0.6 3,106,389 + -0.0 3,106,392 - -1.1 3,106,418 + -1.3 3,106,470 + -0.4 3,106,510 + -0.1 3,106,510 + -0.2 3,106,549 - +0.3 3,106,559 - +0.7 3,106,607 + -0.7 3,106,625 - +1.3 3,106,723 + PGA1_c29700 0.16 +0.2 3,106,788 - PGA1_c29700 0.21 +0.0 3,106,809 - PGA1_c29700 0.22 -0.2 3,106,820 - PGA1_c29700 0.23 +1.9 3,106,820 - PGA1_c29700 0.23 -1.4 3,106,827 + PGA1_c29700 0.24 +1.3 3,106,849 + PGA1_c29700 0.25 -3.2 3,106,849 + PGA1_c29700 0.25 +2.9 3,106,849 + PGA1_c29700 0.25 +0.2 3,106,849 + PGA1_c29700 0.25 +1.4 3,106,849 + PGA1_c29700 0.25 -0.6 3,106,857 - PGA1_c29700 0.26 +1.1 3,106,857 - PGA1_c29700 0.26 -0.9 3,106,871 + PGA1_c29700 0.27 +0.3 3,106,879 - PGA1_c29700 0.28 +0.5 3,106,893 + PGA1_c29700 0.29 +0.0 3,106,901 - PGA1_c29700 0.29 -1.1 3,106,906 + PGA1_c29700 0.30 +2.1 3,106,918 + PGA1_c29700 0.31 -0.5 3,106,926 - PGA1_c29700 0.31 +0.9 3,107,041 - PGA1_c29700 0.40 -0.1 3,107,061 - PGA1_c29700 0.41 -0.3 3,107,079 - PGA1_c29700 0.43 +0.1 3,107,088 + PGA1_c29700 0.43 +0.4 3,107,133 + PGA1_c29700 0.47 -0.8 3,107,230 - PGA1_c29700 0.54 -0.3 3,107,261 - PGA1_c29700 0.56 +0.0 3,107,262 + PGA1_c29700 0.56 -1.3 3,107,280 + PGA1_c29700 0.58 +1.3 3,107,280 + PGA1_c29700 0.58 -0.1 3,107,283 - PGA1_c29700 0.58 -1.5 3,107,330 + PGA1_c29700 0.61 -0.6
Or see this region's nucleotide sequence