Experiment: Phaeo_ML1_MIT diversity barseq
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c26710 and PGA1_c26720 are separated by 98 nucleotides PGA1_c26720 and PGA1_c26730 are separated by 2 nucleotides PGA1_c26730 and PGA1_c26740 are separated by 12 nucleotides
PGA1_c26710: PGA1_c26710 - glutathione synthetase GshB, at 2,785,944 to 2,786,876
_c26710
PGA1_c26720: PGA1_c26720 - Predicted endonuclease distantly related to archaeal Holliday junction resolvase, at 2,786,975 to 2,787,379
_c26720
PGA1_c26730: PGA1_c26730 - probable S-adenosylmethionine-dependent methyltransferase, YraL family, at 2,787,382 to 2,788,242
_c26730
PGA1_c26740: PGA1_c26740 - ABC-type branched-chain amino acid transport systems, periplasmic component, at 2,788,255 to 2,789,499
_c26740
Position (kb)
2787
2788
2789 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2786.896 kb on - strand at 2786.973 kb on + strand at 2786.973 kb on + strand at 2786.978 kb on + strand at 2786.981 kb on - strand at 2786.986 kb on - strand at 2786.991 kb on - strand at 2787.012 kb on - strand at 2787.108 kb on + strand, within PGA1_c26720 at 2787.123 kb on + strand, within PGA1_c26720 at 2787.123 kb on + strand, within PGA1_c26720 at 2787.123 kb on + strand, within PGA1_c26720 at 2787.141 kb on + strand, within PGA1_c26720 at 2787.169 kb on - strand, within PGA1_c26720 at 2787.265 kb on + strand, within PGA1_c26720 at 2787.751 kb on + strand, within PGA1_c26730 at 2787.874 kb on + strand, within PGA1_c26730 at 2787.895 kb on - strand, within PGA1_c26730 at 2787.970 kb on + strand, within PGA1_c26730 at 2787.970 kb on + strand, within PGA1_c26730 at 2787.997 kb on - strand, within PGA1_c26730 at 2788.012 kb on - strand, within PGA1_c26730 at 2788.028 kb on + strand, within PGA1_c26730 at 2788.031 kb on - strand, within PGA1_c26730 at 2788.072 kb on - strand, within PGA1_c26730 at 2788.112 kb on - strand, within PGA1_c26730 at 2788.166 kb on - strand at 2788.181 kb on + strand at 2788.181 kb on + strand at 2788.279 kb on + strand at 2789.190 kb on + strand, within PGA1_c26740
Per-strain Table
Position Strand Gene LocusTag Fraction Phaeo_ML1_MIT diversity barseq remove 2,786,896 - +0.1 2,786,973 + -1.1 2,786,973 + +0.1 2,786,978 + -0.0 2,786,981 - +0.9 2,786,986 - +2.1 2,786,991 - +1.2 2,787,012 - -0.3 2,787,108 + PGA1_c26720 0.33 +0.9 2,787,123 + PGA1_c26720 0.37 +1.6 2,787,123 + PGA1_c26720 0.37 +0.4 2,787,123 + PGA1_c26720 0.37 +0.9 2,787,141 + PGA1_c26720 0.41 -1.4 2,787,169 - PGA1_c26720 0.48 -0.4 2,787,265 + PGA1_c26720 0.72 -0.0 2,787,751 + PGA1_c26730 0.43 +0.2 2,787,874 + PGA1_c26730 0.57 -0.4 2,787,895 - PGA1_c26730 0.60 -0.5 2,787,970 + PGA1_c26730 0.68 -0.9 2,787,970 + PGA1_c26730 0.68 -0.1 2,787,997 - PGA1_c26730 0.71 +0.7 2,788,012 - PGA1_c26730 0.73 -0.5 2,788,028 + PGA1_c26730 0.75 -1.8 2,788,031 - PGA1_c26730 0.75 -0.9 2,788,072 - PGA1_c26730 0.80 +0.1 2,788,112 - PGA1_c26730 0.85 -0.4 2,788,166 - +0.9 2,788,181 + +0.3 2,788,181 + -0.4 2,788,279 + +1.3 2,789,190 + PGA1_c26740 0.75 +0.6
Or see this region's nucleotide sequence