Experiment: Phaeo_ML1_MIT diversity barseq
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c17780 and PGA1_c17790 are separated by 101 nucleotides PGA1_c17790 and PGA1_c17800 overlap by 4 nucleotides PGA1_c17800 and PGA1_c17810 overlap by 4 nucleotides PGA1_c17810 and PGA1_c17820 are separated by 176 nucleotides
PGA1_c17780: PGA1_c17780 - putative gene transfer agent major tail protein, at 1,860,818 to 1,861,231
_c17780
PGA1_c17790: PGA1_c17790 - putative gene transfer agent protein, at 1,861,333 to 1,861,740
_c17790
PGA1_c17800: PGA1_c17800 - putative gene transfer agent head-tail adaptor, at 1,861,737 to 1,862,108
_c17800
PGA1_c17810: PGA1_c17810 - putative gene transfer agent protein, at 1,862,105 to 1,862,755
_c17810
PGA1_c17820: PGA1_c17820 - gene transfer agent major capsid protein, at 1,862,932 to 1,864,116
_c17820
Position (kb) 1862
1863 Strain fitness (log2 ratio) -2
-1
0
1 at 1861.114 kb on - strand, within PGA1_c17780 at 1861.161 kb on - strand, within PGA1_c17780 at 1861.280 kb on + strand at 1861.280 kb on + strand at 1861.283 kb on - strand at 1861.315 kb on + strand at 1861.398 kb on - strand, within PGA1_c17790 at 1861.436 kb on - strand, within PGA1_c17790 at 1861.669 kb on + strand, within PGA1_c17790 at 1861.693 kb on - strand, within PGA1_c17790 at 1861.722 kb on + strand at 1861.728 kb on + strand at 1861.729 kb on + strand at 1861.736 kb on - strand at 1861.792 kb on + strand, within PGA1_c17800 at 1861.936 kb on + strand, within PGA1_c17800 at 1862.055 kb on + strand, within PGA1_c17800 at 1862.172 kb on - strand, within PGA1_c17810 at 1862.187 kb on + strand, within PGA1_c17810 at 1862.294 kb on + strand, within PGA1_c17810 at 1862.335 kb on - strand, within PGA1_c17810 at 1862.458 kb on + strand, within PGA1_c17810 at 1862.540 kb on + strand, within PGA1_c17810 at 1862.544 kb on - strand, within PGA1_c17810 at 1862.656 kb on - strand, within PGA1_c17810 at 1862.716 kb on - strand at 1862.784 kb on - strand at 1862.784 kb on - strand at 1862.810 kb on + strand at 1862.817 kb on - strand at 1862.876 kb on - strand at 1862.897 kb on + strand at 1863.027 kb on - strand at 1863.062 kb on - strand, within PGA1_c17820 at 1863.081 kb on - strand, within PGA1_c17820 at 1863.105 kb on + strand, within PGA1_c17820 at 1863.113 kb on - strand, within PGA1_c17820 at 1863.131 kb on + strand, within PGA1_c17820 at 1863.251 kb on + strand, within PGA1_c17820 at 1863.251 kb on + strand, within PGA1_c17820 at 1863.302 kb on + strand, within PGA1_c17820 at 1863.451 kb on + strand, within PGA1_c17820 at 1863.498 kb on + strand, within PGA1_c17820 at 1863.506 kb on - strand, within PGA1_c17820 at 1863.551 kb on + strand, within PGA1_c17820 at 1863.551 kb on + strand, within PGA1_c17820 at 1863.559 kb on - strand, within PGA1_c17820 at 1863.654 kb on - strand, within PGA1_c17820 at 1863.678 kb on + strand, within PGA1_c17820 at 1863.693 kb on + strand, within PGA1_c17820 at 1863.734 kb on + strand, within PGA1_c17820 at 1863.734 kb on + strand, within PGA1_c17820
Per-strain Table
Position Strand Gene LocusTag Fraction Phaeo_ML1_MIT diversity barseq remove 1,861,114 - PGA1_c17780 0.71 -1.2 1,861,161 - PGA1_c17780 0.83 +0.6 1,861,280 + -0.8 1,861,280 + +1.3 1,861,283 - -0.3 1,861,315 + -2.3 1,861,398 - PGA1_c17790 0.16 -1.6 1,861,436 - PGA1_c17790 0.25 +0.8 1,861,669 + PGA1_c17790 0.82 +1.3 1,861,693 - PGA1_c17790 0.88 -0.1 1,861,722 + -0.7 1,861,728 + +0.5 1,861,729 + -1.4 1,861,736 - +1.6 1,861,792 + PGA1_c17800 0.15 -0.6 1,861,936 + PGA1_c17800 0.53 -0.3 1,862,055 + PGA1_c17800 0.85 +0.5 1,862,172 - PGA1_c17810 0.10 +0.2 1,862,187 + PGA1_c17810 0.13 +0.9 1,862,294 + PGA1_c17810 0.29 -1.3 1,862,335 - PGA1_c17810 0.35 -1.3 1,862,458 + PGA1_c17810 0.54 -0.6 1,862,540 + PGA1_c17810 0.67 -0.6 1,862,544 - PGA1_c17810 0.67 +0.0 1,862,656 - PGA1_c17810 0.85 -0.3 1,862,716 - +0.4 1,862,784 - -0.4 1,862,784 - -0.4 1,862,810 + -0.1 1,862,817 - +0.6 1,862,876 - -0.3 1,862,897 + +1.2 1,863,027 - +0.5 1,863,062 - PGA1_c17820 0.11 -1.0 1,863,081 - PGA1_c17820 0.13 -0.8 1,863,105 + PGA1_c17820 0.15 +1.6 1,863,113 - PGA1_c17820 0.15 -0.6 1,863,131 + PGA1_c17820 0.17 -0.3 1,863,251 + PGA1_c17820 0.27 +0.5 1,863,251 + PGA1_c17820 0.27 -1.0 1,863,302 + PGA1_c17820 0.31 +0.3 1,863,451 + PGA1_c17820 0.44 +0.1 1,863,498 + PGA1_c17820 0.48 +1.6 1,863,506 - PGA1_c17820 0.48 -1.2 1,863,551 + PGA1_c17820 0.52 -1.7 1,863,551 + PGA1_c17820 0.52 -0.2 1,863,559 - PGA1_c17820 0.53 +0.9 1,863,654 - PGA1_c17820 0.61 +0.3 1,863,678 + PGA1_c17820 0.63 -0.7 1,863,693 + PGA1_c17820 0.64 -0.4 1,863,734 + PGA1_c17820 0.68 +0.4 1,863,734 + PGA1_c17820 0.68 -0.0
Or see this region's nucleotide sequence