Experiment: BHIS with Lithocholic 0.4 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT4413 and BT4414 are separated by 220 nucleotides BT4414 and BT4415 are separated by 14 nucleotides BT4415 and BT4416 are separated by 46 nucleotides BT4416 and BT4417 are separated by 122 nucleotides
BT4413: BT4413 - hypothetical protein (NCBI ptt file), at 5,817,124 to 5,817,561
BT4413
BT4414: BT4414 - hypothetical protein (NCBI ptt file), at 5,817,782 to 5,818,333
BT4414
BT4415: BT4415 - putative decarboxylase beta subunit (NCBI ptt file), at 5,818,348 to 5,819,562
BT4415
BT4416: BT4416 - Cu2+ homeostasis protein CutC (NCBI ptt file), at 5,819,609 to 5,820,358
BT4416
BT4417: BT4417 - HD superfamily hydrolase (NCBI ptt file), at 5,820,481 to 5,822,016
BT4417
Position (kb)
5818
5819
5820 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 5817.424 kb on - strand, within BT4413 at 5817.435 kb on - strand, within BT4413 at 5817.435 kb on - strand, within BT4413 at 5817.457 kb on - strand, within BT4413 at 5817.511 kb on - strand, within BT4413 at 5817.511 kb on - strand at 5817.527 kb on - strand at 5817.527 kb on - strand at 5817.527 kb on - strand at 5817.527 kb on - strand at 5817.529 kb on + strand at 5817.535 kb on + strand at 5817.535 kb on + strand at 5817.537 kb on + strand at 5817.538 kb on - strand at 5817.538 kb on - strand at 5817.555 kb on + strand at 5817.614 kb on - strand at 5817.614 kb on - strand at 5817.617 kb on + strand at 5817.626 kb on + strand at 5817.640 kb on - strand at 5817.642 kb on - strand at 5817.715 kb on + strand at 5817.716 kb on - strand at 5817.833 kb on - strand at 5817.873 kb on - strand, within BT4414 at 5817.873 kb on - strand, within BT4414 at 5818.048 kb on - strand, within BT4414 at 5818.188 kb on - strand, within BT4414 at 5818.236 kb on - strand, within BT4414 at 5818.347 kb on + strand at 5818.458 kb on + strand at 5818.459 kb on - strand at 5818.491 kb on + strand, within BT4415 at 5818.492 kb on - strand, within BT4415 at 5818.561 kb on - strand, within BT4415 at 5818.566 kb on - strand, within BT4415 at 5818.628 kb on + strand, within BT4415 at 5818.674 kb on + strand, within BT4415 at 5818.676 kb on + strand, within BT4415 at 5818.751 kb on + strand, within BT4415 at 5818.763 kb on + strand, within BT4415 at 5818.764 kb on - strand, within BT4415 at 5818.846 kb on - strand, within BT4415 at 5818.970 kb on - strand, within BT4415 at 5818.982 kb on + strand, within BT4415 at 5819.010 kb on + strand, within BT4415 at 5819.010 kb on + strand, within BT4415 at 5819.011 kb on - strand, within BT4415 at 5819.042 kb on - strand, within BT4415 at 5819.172 kb on + strand, within BT4415 at 5819.173 kb on - strand, within BT4415 at 5819.176 kb on + strand, within BT4415 at 5819.218 kb on - strand, within BT4415 at 5819.294 kb on + strand, within BT4415 at 5819.344 kb on + strand, within BT4415 at 5819.354 kb on - strand, within BT4415 at 5819.374 kb on + strand, within BT4415 at 5819.404 kb on - strand, within BT4415 at 5819.412 kb on - strand, within BT4415 at 5819.416 kb on - strand, within BT4415 at 5819.444 kb on + strand at 5819.445 kb on - strand at 5819.496 kb on + strand at 5819.537 kb on - strand at 5819.537 kb on - strand at 5819.538 kb on + strand at 5819.554 kb on + strand at 5819.569 kb on - strand at 5819.569 kb on - strand at 5819.657 kb on + strand at 5819.658 kb on - strand at 5819.684 kb on + strand, within BT4416 at 5819.685 kb on - strand, within BT4416 at 5819.702 kb on + strand, within BT4416 at 5819.833 kb on + strand, within BT4416 at 5819.833 kb on + strand, within BT4416 at 5819.911 kb on + strand, within BT4416 at 5819.918 kb on - strand, within BT4416 at 5819.973 kb on - strand, within BT4416 at 5819.974 kb on + strand, within BT4416 at 5820.150 kb on + strand, within BT4416 at 5820.273 kb on - strand, within BT4416 at 5820.380 kb on - strand at 5820.455 kb on + strand at 5820.475 kb on - strand at 5820.478 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Lithocholic 0.4 mg/ml remove 5,817,424 - BT4413 0.68 -0.5 5,817,435 - BT4413 0.71 -1.9 5,817,435 - BT4413 0.71 -1.9 5,817,457 - BT4413 0.76 -1.9 5,817,511 - BT4413 0.88 -0.1 5,817,511 - -0.5 5,817,527 - +1.1 5,817,527 - +0.3 5,817,527 - -2.1 5,817,527 - -2.1 5,817,529 + -1.2 5,817,535 + -0.2 5,817,535 + -1.9 5,817,537 + -1.9 5,817,538 - -1.5 5,817,538 - -2.3 5,817,555 + -0.7 5,817,614 - -1.9 5,817,614 - -0.9 5,817,617 + +0.1 5,817,626 + -1.8 5,817,640 - +0.9 5,817,642 - -2.2 5,817,715 + -0.3 5,817,716 - -1.4 5,817,833 - -1.7 5,817,873 - BT4414 0.16 -1.3 5,817,873 - BT4414 0.16 -2.3 5,818,048 - BT4414 0.48 -1.5 5,818,188 - BT4414 0.74 +0.4 5,818,236 - BT4414 0.82 -1.2 5,818,347 + -2.1 5,818,458 + -0.1 5,818,459 - +0.1 5,818,491 + BT4415 0.12 -0.3 5,818,492 - BT4415 0.12 -1.5 5,818,561 - BT4415 0.18 -0.5 5,818,566 - BT4415 0.18 -2.5 5,818,628 + BT4415 0.23 +0.4 5,818,674 + BT4415 0.27 -1.7 5,818,676 + BT4415 0.27 -2.1 5,818,751 + BT4415 0.33 -0.4 5,818,763 + BT4415 0.34 -1.5 5,818,764 - BT4415 0.34 -2.2 5,818,846 - BT4415 0.41 -1.7 5,818,970 - BT4415 0.51 -2.1 5,818,982 + BT4415 0.52 -0.9 5,819,010 + BT4415 0.54 -2.6 5,819,010 + BT4415 0.54 -1.2 5,819,011 - BT4415 0.55 -2.3 5,819,042 - BT4415 0.57 -0.9 5,819,172 + BT4415 0.68 -2.9 5,819,173 - BT4415 0.68 -0.6 5,819,176 + BT4415 0.68 -3.4 5,819,218 - BT4415 0.72 -3.1 5,819,294 + BT4415 0.78 -3.2 5,819,344 + BT4415 0.82 -0.9 5,819,354 - BT4415 0.83 -2.7 5,819,374 + BT4415 0.84 -0.7 5,819,404 - BT4415 0.87 -0.9 5,819,412 - BT4415 0.88 -0.7 5,819,416 - BT4415 0.88 +0.7 5,819,444 + -1.5 5,819,445 - -1.2 5,819,496 + -0.9 5,819,537 - -2.8 5,819,537 - -2.8 5,819,538 + -2.1 5,819,554 + -1.7 5,819,569 - -2.7 5,819,569 - -0.4 5,819,657 + -0.9 5,819,658 - -2.6 5,819,684 + BT4416 0.10 -1.5 5,819,685 - BT4416 0.10 +1.1 5,819,702 + BT4416 0.12 +2.4 5,819,833 + BT4416 0.30 -1.2 5,819,833 + BT4416 0.30 +1.2 5,819,911 + BT4416 0.40 -0.2 5,819,918 - BT4416 0.41 -0.9 5,819,973 - BT4416 0.49 +0.8 5,819,974 + BT4416 0.49 -2.3 5,820,150 + BT4416 0.72 -1.2 5,820,273 - BT4416 0.89 +0.1 5,820,380 - -1.7 5,820,455 + -1.2 5,820,475 - +0.2 5,820,478 + +0.4
Or see this region's nucleotide sequence