Strain Fitness in Bacteroides thetaiotaomicron VPI-5482 around BT3214

Experiment: BHIS with Lithocholic 0.4 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBT3212 and BT3213 are separated by 52 nucleotidesBT3213 and BT3214 are separated by 17 nucleotidesBT3214 and BT3215 are separated by 16 nucleotidesBT3215 and BT3216 are separated by 7 nucleotidesBT3216 and BT3217 are separated by 30 nucleotides BT3212: BT3212 - putative bacitracin resistance protein (NCBI ptt file), at 4,104,984 to 4,105,778 BT3212 BT3213: BT3213 - tRNA pseudouridine synthase B (NCBI ptt file), at 4,105,831 to 4,106,553 BT3213 BT3214: BT3214 - S-adenosylmethionine:tRNA ribosyltransferase-isomerase (NCBI ptt file), at 4,106,571 to 4,107,629 BT3214 BT3215: BT3215 - conserved hypothetical protein (NCBI ptt file), at 4,107,646 to 4,107,981 BT3215 BT3216: BT3216 - 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine (NCBI ptt file), at 4,107,989 to 4,108,471 BT3216 BT3217: BT3217 - hypothetical protein (NCBI ptt file), at 4,108,502 to 4,108,693 BT3217 Position (kb) 4106 4107 4108Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 4105.793 kb on + strandat 4105.898 kb on + strandat 4105.952 kb on + strand, within BT3213at 4106.306 kb on + strand, within BT3213at 4106.306 kb on + strand, within BT3213at 4106.380 kb on + strand, within BT3213at 4106.556 kb on + strandat 4107.684 kb on + strand, within BT3215at 4107.684 kb on + strandat 4107.712 kb on + strand, within BT3215at 4107.734 kb on + strand, within BT3215at 4107.907 kb on + strand, within BT3215at 4107.932 kb on + strand, within BT3215at 4107.932 kb on + strand, within BT3215at 4107.974 kb on + strandat 4107.974 kb on + strandat 4108.563 kb on - strand, within BT3217at 4108.571 kb on + strand, within BT3217at 4108.571 kb on + strand, within BT3217at 4108.572 kb on - strand, within BT3217at 4108.583 kb on - strand, within BT3217at 4108.613 kb on + strand, within BT3217at 4108.614 kb on - strand, within BT3217at 4108.624 kb on - strand, within BT3217

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Per-strain Table

Position Strand Gene LocusTag Fraction BHIS with Lithocholic 0.4 mg/ml
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4,105,793 + -3.3
4,105,898 + +0.2
4,105,952 + BT3213 0.17 -1.2
4,106,306 + BT3213 0.66 +0.4
4,106,306 + BT3213 0.66 +0.4
4,106,380 + BT3213 0.76 -1.6
4,106,556 + -0.2
4,107,684 + BT3215 0.11 -0.8
4,107,684 + -1.6
4,107,712 + BT3215 0.20 -0.9
4,107,734 + BT3215 0.26 -0.4
4,107,907 + BT3215 0.78 -0.0
4,107,932 + BT3215 0.85 -0.3
4,107,932 + BT3215 0.85 -2.6
4,107,974 + -1.4
4,107,974 + +1.4
4,108,563 - BT3217 0.32 -2.7
4,108,571 + BT3217 0.36 -1.5
4,108,571 + BT3217 0.36 -0.1
4,108,572 - BT3217 0.36 +2.7
4,108,583 - BT3217 0.42 -0.9
4,108,613 + BT3217 0.58 +0.4
4,108,614 - BT3217 0.58 -1.8
4,108,624 - BT3217 0.64 -1.7

Or see this region's nucleotide sequence