Experiment: BHIS with Lithocholic 0.4 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT3210 and BT3211 are separated by 49 nucleotides BT3211 and BT3212 are separated by 12 nucleotides BT3212 and BT3213 are separated by 52 nucleotides BT3213 and BT3214 are separated by 17 nucleotides
BT3210: BT3210 - cell division protein FtsX (NCBI ptt file), at 4,103,807 to 4,104,688
BT3210
BT3211: BT3211 - conserved hypothetical protein (NCBI ptt file), at 4,104,738 to 4,104,971
BT3211
BT3212: BT3212 - putative bacitracin resistance protein (NCBI ptt file), at 4,104,984 to 4,105,778
BT3212
BT3213: BT3213 - tRNA pseudouridine synthase B (NCBI ptt file), at 4,105,831 to 4,106,553
BT3213
BT3214: BT3214 - S-adenosylmethionine:tRNA ribosyltransferase-isomerase (NCBI ptt file), at 4,106,571 to 4,107,629
BT3214
Position (kb)
4104
4105
4106 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 4104.004 kb on + strand, within BT3210 at 4104.234 kb on + strand, within BT3210 at 4104.285 kb on + strand, within BT3210 at 4104.334 kb on + strand, within BT3210 at 4104.936 kb on + strand, within BT3211 at 4104.936 kb on + strand, within BT3211 at 4104.950 kb on + strand at 4105.793 kb on + strand at 4105.898 kb on + strand at 4105.952 kb on + strand, within BT3213 at 4106.306 kb on + strand, within BT3213 at 4106.306 kb on + strand, within BT3213 at 4106.380 kb on + strand, within BT3213 at 4106.556 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Lithocholic 0.4 mg/ml remove 4,104,004 + BT3210 0.22 -1.8 4,104,234 + BT3210 0.48 -0.6 4,104,285 + BT3210 0.54 -3.5 4,104,334 + BT3210 0.60 +1.4 4,104,936 + BT3211 0.85 +1.1 4,104,936 + BT3211 0.85 +1.4 4,104,950 + -0.9 4,105,793 + -3.3 4,105,898 + +0.2 4,105,952 + BT3213 0.17 -1.2 4,106,306 + BT3213 0.66 +0.4 4,106,306 + BT3213 0.66 +0.4 4,106,380 + BT3213 0.76 -1.6 4,106,556 + -0.2
Or see this region's nucleotide sequence