Experiment: BHIS with Lithocholic 0.4 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT3209 and BT3210 are separated by 3 nucleotides BT3210 and BT3211 are separated by 49 nucleotides BT3211 and BT3212 are separated by 12 nucleotides BT3212 and BT3213 are separated by 52 nucleotides
BT3209: BT3209 - conserved hypothetical protein (NCBI ptt file), at 4,102,964 to 4,103,803
BT3209
BT3210: BT3210 - cell division protein FtsX (NCBI ptt file), at 4,103,807 to 4,104,688
BT3210
BT3211: BT3211 - conserved hypothetical protein (NCBI ptt file), at 4,104,738 to 4,104,971
BT3211
BT3212: BT3212 - putative bacitracin resistance protein (NCBI ptt file), at 4,104,984 to 4,105,778
BT3212
BT3213: BT3213 - tRNA pseudouridine synthase B (NCBI ptt file), at 4,105,831 to 4,106,553
BT3213
Position (kb)
4104
4105 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 4103.825 kb on + strand at 4103.827 kb on + strand at 4103.827 kb on + strand at 4103.848 kb on + strand at 4103.869 kb on + strand at 4103.935 kb on + strand, within BT3210 at 4103.943 kb on + strand, within BT3210 at 4104.004 kb on + strand, within BT3210 at 4104.234 kb on + strand, within BT3210 at 4104.285 kb on + strand, within BT3210 at 4104.334 kb on + strand, within BT3210 at 4104.936 kb on + strand, within BT3211 at 4104.936 kb on + strand, within BT3211 at 4104.950 kb on + strand at 4105.793 kb on + strand at 4105.898 kb on + strand at 4105.952 kb on + strand, within BT3213
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Lithocholic 0.4 mg/ml remove 4,103,825 + +0.5 4,103,827 + -0.2 4,103,827 + +0.6 4,103,848 + -1.1 4,103,869 + +0.2 4,103,935 + BT3210 0.15 +0.4 4,103,943 + BT3210 0.15 -3.1 4,104,004 + BT3210 0.22 -1.8 4,104,234 + BT3210 0.48 -0.6 4,104,285 + BT3210 0.54 -3.5 4,104,334 + BT3210 0.60 +1.4 4,104,936 + BT3211 0.85 +1.1 4,104,936 + BT3211 0.85 +1.4 4,104,950 + -0.9 4,105,793 + -3.3 4,105,898 + +0.2 4,105,952 + BT3213 0.17 -1.2
Or see this region's nucleotide sequence