Experiment: BHIS with Lithocholic 0.4 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT2836 and BT2837 are separated by 3 nucleotides BT2837 and BT2838 are separated by 181 nucleotides
BT2836: BT2836 - 2-oxoglutarate synthase subunit korA (NCBI ptt file), at 3,537,850 to 3,539,700
BT2836
BT2837: BT2837 - 2-oxoglutarate synthase subunit korB (NCBI ptt file), at 3,539,704 to 3,540,714
BT2837
BT2838: BT2838 - conserved hypothetical protein (NCBI ptt file), at 3,540,896 to 3,542,122
BT2838
Position (kb)
3539
3540
3541 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 3538.781 kb on + strand, within BT2836 at 3538.871 kb on + strand, within BT2836 at 3539.054 kb on + strand, within BT2836 at 3539.054 kb on + strand, within BT2836 at 3539.119 kb on + strand at 3539.145 kb on - strand, within BT2836 at 3539.145 kb on - strand, within BT2836 at 3539.185 kb on - strand, within BT2836 at 3539.236 kb on - strand, within BT2836 at 3539.265 kb on + strand, within BT2836 at 3539.353 kb on + strand, within BT2836 at 3539.354 kb on - strand, within BT2836 at 3539.354 kb on - strand, within BT2836 at 3539.354 kb on - strand at 3539.396 kb on + strand at 3539.396 kb on + strand, within BT2836 at 3539.397 kb on - strand, within BT2836 at 3539.405 kb on + strand, within BT2836 at 3539.405 kb on + strand, within BT2836 at 3539.422 kb on + strand, within BT2836 at 3539.422 kb on + strand, within BT2836 at 3539.423 kb on - strand, within BT2836 at 3539.443 kb on - strand, within BT2836 at 3539.495 kb on - strand, within BT2836 at 3539.528 kb on + strand at 3539.531 kb on + strand at 3539.531 kb on + strand at 3539.535 kb on + strand at 3539.535 kb on + strand at 3539.535 kb on + strand at 3539.535 kb on + strand at 3539.536 kb on - strand at 3539.540 kb on - strand at 3539.552 kb on + strand at 3539.553 kb on - strand at 3539.553 kb on - strand at 3539.553 kb on - strand at 3539.556 kb on - strand at 3539.595 kb on + strand at 3539.617 kb on - strand at 3539.656 kb on - strand at 3539.669 kb on + strand at 3539.670 kb on - strand at 3539.670 kb on - strand at 3539.670 kb on - strand at 3539.670 kb on - strand at 3539.670 kb on - strand at 3539.698 kb on + strand at 3539.699 kb on - strand at 3539.699 kb on - strand at 3539.717 kb on + strand at 3539.717 kb on + strand at 3539.721 kb on + strand at 3539.721 kb on + strand at 3539.722 kb on - strand at 3539.722 kb on - strand at 3539.736 kb on + strand at 3539.822 kb on + strand, within BT2837 at 3539.822 kb on + strand, within BT2837 at 3539.822 kb on + strand, within BT2837 at 3539.836 kb on - strand, within BT2837 at 3539.878 kb on + strand, within BT2837 at 3539.881 kb on + strand at 3539.882 kb on - strand, within BT2837 at 3539.885 kb on + strand, within BT2837 at 3539.886 kb on - strand, within BT2837 at 3539.889 kb on + strand, within BT2837 at 3539.889 kb on + strand, within BT2837 at 3539.889 kb on + strand, within BT2837 at 3539.889 kb on + strand at 3539.890 kb on - strand, within BT2837 at 3539.890 kb on - strand, within BT2837 at 3539.893 kb on + strand, within BT2837 at 3539.893 kb on + strand, within BT2837 at 3539.968 kb on - strand, within BT2837 at 3540.075 kb on + strand, within BT2837 at 3540.075 kb on + strand, within BT2837 at 3540.076 kb on - strand, within BT2837 at 3540.076 kb on - strand, within BT2837 at 3540.079 kb on + strand, within BT2837 at 3540.101 kb on - strand, within BT2837 at 3540.146 kb on - strand, within BT2837 at 3540.219 kb on + strand, within BT2837 at 3540.220 kb on - strand, within BT2837 at 3540.220 kb on - strand, within BT2837 at 3540.273 kb on + strand, within BT2837 at 3540.526 kb on - strand, within BT2837 at 3540.526 kb on - strand, within BT2837 at 3540.606 kb on + strand, within BT2837 at 3540.606 kb on + strand, within BT2837 at 3540.606 kb on + strand, within BT2837 at 3540.606 kb on + strand, within BT2837 at 3540.607 kb on - strand, within BT2837 at 3540.610 kb on + strand, within BT2837 at 3540.611 kb on - strand, within BT2837 at 3540.611 kb on - strand, within BT2837 at 3540.661 kb on + strand at 3540.696 kb on + strand at 3540.788 kb on - strand at 3540.798 kb on + strand at 3540.798 kb on + strand at 3540.799 kb on - strand at 3540.839 kb on + strand at 3540.850 kb on + strand at 3540.851 kb on - strand at 3540.851 kb on - strand at 3540.861 kb on - strand at 3540.897 kb on + strand at 3540.898 kb on - strand at 3540.898 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Lithocholic 0.4 mg/ml remove 3,538,781 + BT2836 0.50 +0.3 3,538,871 + BT2836 0.55 -2.3 3,539,054 + BT2836 0.65 -0.8 3,539,054 + BT2836 0.65 -1.8 3,539,119 + -1.0 3,539,145 - BT2836 0.70 +0.3 3,539,145 - BT2836 0.70 -2.0 3,539,185 - BT2836 0.72 -0.7 3,539,236 - BT2836 0.75 -1.0 3,539,265 + BT2836 0.76 -1.2 3,539,353 + BT2836 0.81 -0.2 3,539,354 - BT2836 0.81 -1.6 3,539,354 - BT2836 0.81 -1.8 3,539,354 - +1.3 3,539,396 + -1.0 3,539,396 + BT2836 0.84 -2.1 3,539,397 - BT2836 0.84 -0.8 3,539,405 + BT2836 0.84 -1.2 3,539,405 + BT2836 0.84 -3.0 3,539,422 + BT2836 0.85 -1.4 3,539,422 + BT2836 0.85 -0.7 3,539,423 - BT2836 0.85 -2.8 3,539,443 - BT2836 0.86 -1.7 3,539,495 - BT2836 0.89 -1.7 3,539,528 + -3.1 3,539,531 + -3.5 3,539,531 + -0.2 3,539,535 + -2.7 3,539,535 + +0.3 3,539,535 + -1.0 3,539,535 + +0.3 3,539,536 - -0.1 3,539,540 - -0.7 3,539,552 + -2.7 3,539,553 - -1.1 3,539,553 - +0.3 3,539,553 - -1.2 3,539,556 - -2.9 3,539,595 + -3.6 3,539,617 - +0.3 3,539,656 - +2.9 3,539,669 + -0.1 3,539,670 - -3.0 3,539,670 - -0.7 3,539,670 - -4.2 3,539,670 - -2.1 3,539,670 - -1.4 3,539,698 + -1.5 3,539,699 - -1.1 3,539,699 - +0.3 3,539,717 + +0.1 3,539,717 + -3.5 3,539,721 + +1.3 3,539,721 + -3.3 3,539,722 - -2.8 3,539,722 - -1.8 3,539,736 + -1.7 3,539,822 + BT2837 0.12 -1.8 3,539,822 + BT2837 0.12 -2.1 3,539,822 + BT2837 0.12 -1.5 3,539,836 - BT2837 0.13 -3.2 3,539,878 + BT2837 0.17 -1.8 3,539,881 + +0.3 3,539,882 - BT2837 0.18 +0.3 3,539,885 + BT2837 0.18 +1.3 3,539,886 - BT2837 0.18 -2.0 3,539,889 + BT2837 0.18 -3.1 3,539,889 + BT2837 0.18 -1.9 3,539,889 + BT2837 0.18 -1.7 3,539,889 + +0.3 3,539,890 - BT2837 0.18 -2.5 3,539,890 - BT2837 0.18 -1.8 3,539,893 + BT2837 0.19 -1.8 3,539,893 + BT2837 0.19 -2.1 3,539,968 - BT2837 0.26 -0.7 3,540,075 + BT2837 0.37 -2.8 3,540,075 + BT2837 0.37 -1.0 3,540,076 - BT2837 0.37 -1.7 3,540,076 - BT2837 0.37 -1.0 3,540,079 + BT2837 0.37 -1.2 3,540,101 - BT2837 0.39 -2.1 3,540,146 - BT2837 0.44 -1.2 3,540,219 + BT2837 0.51 -2.9 3,540,220 - BT2837 0.51 -1.2 3,540,220 - BT2837 0.51 -0.8 3,540,273 + BT2837 0.56 -0.7 3,540,526 - BT2837 0.81 -1.8 3,540,526 - BT2837 0.81 -1.0 3,540,606 + BT2837 0.89 -1.8 3,540,606 + BT2837 0.89 -2.8 3,540,606 + BT2837 0.89 +0.0 3,540,606 + BT2837 0.89 -2.8 3,540,607 - BT2837 0.89 +0.3 3,540,610 + BT2837 0.90 -1.6 3,540,611 - BT2837 0.90 -3.4 3,540,611 - BT2837 0.90 -0.7 3,540,661 + -2.1 3,540,696 + -1.7 3,540,788 - +0.5 3,540,798 + -0.8 3,540,798 + +0.4 3,540,799 - -1.5 3,540,839 + -2.1 3,540,850 + -0.1 3,540,851 - -1.0 3,540,851 - -2.1 3,540,861 - -2.2 3,540,897 + +0.3 3,540,898 - -0.2 3,540,898 - -2.6
Or see this region's nucleotide sequence