Experiment: BHIS with Lithocholic 0.4 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT2279 and BT2280 are separated by 1 nucleotides BT2280 and BT2281 are separated by 186 nucleotides
BT2279: BT2279 - putative ribose phosphate pyrophosphokinase (NCBI ptt file), at 2,859,673 to 2,860,593
BT2279
BT2280: BT2280 - hypothetical protein (NCBI ptt file), at 2,860,595 to 2,860,969
BT2280
BT2281: BT2281 - hypothetical protein (NCBI ptt file), at 2,861,156 to 2,861,428
BT2281
Position (kb)
2860
2861 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2859.664 kb on - strand at 2859.678 kb on - strand at 2859.736 kb on + strand at 2859.792 kb on + strand, within BT2279 at 2859.799 kb on + strand, within BT2279 at 2859.800 kb on - strand, within BT2279 at 2859.857 kb on - strand, within BT2279 at 2859.858 kb on + strand, within BT2279 at 2859.858 kb on + strand, within BT2279 at 2859.858 kb on + strand, within BT2279 at 2859.863 kb on + strand, within BT2279 at 2859.864 kb on - strand, within BT2279 at 2859.915 kb on + strand, within BT2279 at 2859.925 kb on - strand, within BT2279 at 2859.981 kb on + strand, within BT2279 at 2859.981 kb on + strand, within BT2279 at 2859.982 kb on - strand, within BT2279 at 2859.984 kb on - strand, within BT2279 at 2859.984 kb on - strand, within BT2279 at 2859.994 kb on - strand, within BT2279 at 2860.030 kb on + strand, within BT2279 at 2860.081 kb on + strand, within BT2279 at 2860.082 kb on - strand, within BT2279 at 2860.093 kb on + strand, within BT2279 at 2860.152 kb on - strand, within BT2279 at 2860.156 kb on - strand, within BT2279 at 2860.161 kb on + strand, within BT2279 at 2860.466 kb on + strand, within BT2279 at 2860.479 kb on + strand, within BT2279 at 2860.479 kb on + strand, within BT2279 at 2860.668 kb on + strand, within BT2280 at 2860.668 kb on + strand, within BT2280 at 2860.669 kb on - strand, within BT2280 at 2860.810 kb on - strand, within BT2280 at 2860.856 kb on - strand, within BT2280 at 2860.882 kb on - strand, within BT2280 at 2860.912 kb on + strand, within BT2280 at 2860.979 kb on + strand at 2860.980 kb on - strand at 2860.980 kb on - strand at 2860.980 kb on - strand at 2860.982 kb on + strand at 2860.982 kb on + strand at 2861.036 kb on + strand at 2861.059 kb on + strand at 2861.093 kb on + strand at 2861.093 kb on + strand at 2861.094 kb on - strand at 2861.094 kb on - strand at 2861.142 kb on - strand at 2861.142 kb on - strand at 2861.182 kb on + strand at 2861.182 kb on + strand at 2861.186 kb on + strand, within BT2281 at 2861.197 kb on - strand, within BT2281 at 2861.304 kb on - strand, within BT2281 at 2861.342 kb on - strand, within BT2281 at 2861.367 kb on + strand, within BT2281 at 2861.367 kb on + strand, within BT2281 at 2861.428 kb on + strand at 2861.440 kb on + strand at 2861.441 kb on - strand at 2861.444 kb on - strand at 2861.448 kb on + strand at 2861.521 kb on + strand at 2861.536 kb on - strand at 2861.536 kb on - strand at 2861.536 kb on - strand at 2861.536 kb on - strand at 2861.536 kb on - strand at 2861.561 kb on + strand at 2861.617 kb on - strand at 2861.617 kb on - strand at 2861.668 kb on - strand at 2861.668 kb on - strand at 2861.671 kb on + strand at 2861.683 kb on - strand at 2861.715 kb on + strand at 2861.952 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Lithocholic 0.4 mg/ml remove 2,859,664 - -0.1 2,859,678 - -0.9 2,859,736 + -1.1 2,859,792 + BT2279 0.13 -0.5 2,859,799 + BT2279 0.14 -2.8 2,859,800 - BT2279 0.14 +0.4 2,859,857 - BT2279 0.20 -2.1 2,859,858 + BT2279 0.20 -0.7 2,859,858 + BT2279 0.20 -1.5 2,859,858 + BT2279 0.20 -0.1 2,859,863 + BT2279 0.21 -1.5 2,859,864 - BT2279 0.21 -2.5 2,859,915 + BT2279 0.26 -0.9 2,859,925 - BT2279 0.27 +0.1 2,859,981 + BT2279 0.33 +0.3 2,859,981 + BT2279 0.33 -0.7 2,859,982 - BT2279 0.34 -1.7 2,859,984 - BT2279 0.34 +0.3 2,859,984 - BT2279 0.34 -2.1 2,859,994 - BT2279 0.35 +0.3 2,860,030 + BT2279 0.39 -1.1 2,860,081 + BT2279 0.44 -0.7 2,860,082 - BT2279 0.44 -0.5 2,860,093 + BT2279 0.46 -2.6 2,860,152 - BT2279 0.52 -0.7 2,860,156 - BT2279 0.52 -0.3 2,860,161 + BT2279 0.53 -2.2 2,860,466 + BT2279 0.86 -1.3 2,860,479 + BT2279 0.88 +0.5 2,860,479 + BT2279 0.88 -1.0 2,860,668 + BT2280 0.19 +1.3 2,860,668 + BT2280 0.19 -1.9 2,860,669 - BT2280 0.20 +0.0 2,860,810 - BT2280 0.57 -2.1 2,860,856 - BT2280 0.70 +0.3 2,860,882 - BT2280 0.77 -1.5 2,860,912 + BT2280 0.85 -2.1 2,860,979 + -0.5 2,860,980 - +0.0 2,860,980 - +1.0 2,860,980 - -2.1 2,860,982 + -0.2 2,860,982 + -1.4 2,861,036 + -2.2 2,861,059 + +0.5 2,861,093 + +0.3 2,861,093 + -0.2 2,861,094 - +1.3 2,861,094 - -0.3 2,861,142 - +0.1 2,861,142 - -0.7 2,861,182 + +0.7 2,861,182 + +0.6 2,861,186 + BT2281 0.11 -1.7 2,861,197 - BT2281 0.15 +2.3 2,861,304 - BT2281 0.54 -1.8 2,861,342 - BT2281 0.68 -1.1 2,861,367 + BT2281 0.77 +1.3 2,861,367 + BT2281 0.77 -0.5 2,861,428 + -0.7 2,861,440 + +0.0 2,861,441 - -2.3 2,861,444 - +0.3 2,861,448 + +0.1 2,861,521 + -2.9 2,861,536 - +0.7 2,861,536 - -1.7 2,861,536 - -1.1 2,861,536 - -0.7 2,861,536 - -0.3 2,861,561 + -0.5 2,861,617 - -0.3 2,861,617 - +0.2 2,861,668 - -1.3 2,861,668 - -3.4 2,861,671 + -1.1 2,861,683 - -0.3 2,861,715 + -1.1 2,861,952 - +0.3
Or see this region's nucleotide sequence