Experiment: BHIS with Lithocholic 0.4 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1573 and BT1574 are separated by 99 nucleotides BT1574 and BT1575 are separated by 209 nucleotides
BT1573: BT1573 - conserved hypothetical protein (NCBI ptt file), at 1,936,538 to 1,937,827
BT1573
BT1574: BT1574 - putative outer membrane protein precursor (NCBI ptt file), at 1,937,927 to 1,939,360
BT1574
BT1575: BT1575 - putative dehydrogenase (NCBI ptt file), at 1,939,570 to 1,940,571
BT1575
Position (kb)
1937
1938
1939
1940 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1937.044 kb on - strand, within BT1573 at 1937.061 kb on - strand, within BT1573 at 1937.066 kb on + strand, within BT1573 at 1937.159 kb on + strand, within BT1573 at 1937.159 kb on + strand, within BT1573 at 1937.160 kb on - strand, within BT1573 at 1937.225 kb on - strand, within BT1573 at 1937.241 kb on + strand, within BT1573 at 1937.246 kb on - strand, within BT1573 at 1937.288 kb on + strand, within BT1573 at 1937.289 kb on - strand, within BT1573 at 1937.315 kb on - strand, within BT1573 at 1937.354 kb on - strand, within BT1573 at 1937.580 kb on - strand, within BT1573 at 1937.615 kb on + strand, within BT1573 at 1937.616 kb on - strand, within BT1573 at 1937.649 kb on - strand, within BT1573 at 1937.688 kb on - strand, within BT1573 at 1937.733 kb on + strand at 1937.817 kb on - strand at 1937.834 kb on - strand at 1937.839 kb on + strand at 1937.841 kb on + strand at 1937.853 kb on + strand at 1937.855 kb on + strand at 1937.906 kb on + strand at 1937.906 kb on + strand at 1937.936 kb on - strand at 1937.969 kb on - strand at 1937.976 kb on + strand at 1937.977 kb on - strand at 1938.034 kb on + strand at 1938.034 kb on + strand at 1938.050 kb on - strand at 1938.060 kb on + strand at 1938.071 kb on + strand, within BT1574 at 1938.072 kb on - strand, within BT1574 at 1938.085 kb on + strand, within BT1574 at 1938.121 kb on - strand, within BT1574 at 1938.216 kb on - strand, within BT1574 at 1938.228 kb on - strand, within BT1574 at 1938.320 kb on + strand, within BT1574 at 1938.351 kb on + strand, within BT1574 at 1938.369 kb on - strand, within BT1574 at 1938.373 kb on + strand, within BT1574 at 1938.394 kb on + strand, within BT1574 at 1938.411 kb on + strand, within BT1574 at 1938.411 kb on + strand, within BT1574 at 1938.421 kb on + strand, within BT1574 at 1938.454 kb on + strand, within BT1574 at 1938.455 kb on - strand, within BT1574 at 1938.461 kb on - strand, within BT1574 at 1938.478 kb on + strand, within BT1574 at 1938.495 kb on + strand, within BT1574 at 1938.593 kb on - strand, within BT1574 at 1938.613 kb on + strand, within BT1574 at 1938.694 kb on + strand, within BT1574 at 1938.717 kb on - strand, within BT1574 at 1938.815 kb on - strand, within BT1574 at 1938.817 kb on - strand, within BT1574 at 1938.840 kb on + strand, within BT1574 at 1938.879 kb on + strand, within BT1574 at 1938.879 kb on + strand, within BT1574 at 1938.883 kb on + strand, within BT1574 at 1938.956 kb on - strand, within BT1574 at 1939.029 kb on - strand, within BT1574 at 1939.037 kb on + strand, within BT1574 at 1939.103 kb on - strand, within BT1574 at 1939.167 kb on - strand, within BT1574 at 1939.211 kb on - strand, within BT1574 at 1939.214 kb on - strand, within BT1574 at 1939.286 kb on - strand at 1939.395 kb on + strand at 1939.419 kb on + strand at 1939.419 kb on + strand at 1939.433 kb on + strand at 1939.566 kb on + strand at 1939.664 kb on - strand at 1939.815 kb on + strand, within BT1575 at 1939.816 kb on - strand, within BT1575 at 1939.900 kb on - strand, within BT1575 at 1939.900 kb on - strand, within BT1575 at 1940.357 kb on - strand, within BT1575
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Lithocholic 0.4 mg/ml remove 1,937,044 - BT1573 0.39 +1.1 1,937,061 - BT1573 0.41 -1.8 1,937,066 + BT1573 0.41 -2.4 1,937,159 + BT1573 0.48 -1.2 1,937,159 + BT1573 0.48 +0.1 1,937,160 - BT1573 0.48 +0.8 1,937,225 - BT1573 0.53 +0.2 1,937,241 + BT1573 0.54 -0.8 1,937,246 - BT1573 0.55 -1.4 1,937,288 + BT1573 0.58 -2.1 1,937,289 - BT1573 0.58 -1.2 1,937,315 - BT1573 0.60 -2.1 1,937,354 - BT1573 0.63 +1.7 1,937,580 - BT1573 0.81 -0.9 1,937,615 + BT1573 0.83 +1.1 1,937,616 - BT1573 0.84 -1.1 1,937,649 - BT1573 0.86 -0.5 1,937,688 - BT1573 0.89 +0.2 1,937,733 + -0.3 1,937,817 - -2.1 1,937,834 - -1.2 1,937,839 + +0.7 1,937,841 + -0.9 1,937,853 + -2.1 1,937,855 + -1.7 1,937,906 + +0.5 1,937,906 + -1.3 1,937,936 - +0.0 1,937,969 - -1.7 1,937,976 + -0.5 1,937,977 - -1.2 1,938,034 + -2.2 1,938,034 + -0.5 1,938,050 - -0.5 1,938,060 + -1.1 1,938,071 + BT1574 0.10 -2.3 1,938,072 - BT1574 0.10 -2.8 1,938,085 + BT1574 0.11 -1.9 1,938,121 - BT1574 0.14 -1.9 1,938,216 - BT1574 0.20 +0.6 1,938,228 - BT1574 0.21 +0.1 1,938,320 + BT1574 0.27 -0.9 1,938,351 + BT1574 0.30 -0.9 1,938,369 - BT1574 0.31 -1.2 1,938,373 + BT1574 0.31 +0.1 1,938,394 + BT1574 0.33 -2.4 1,938,411 + BT1574 0.34 -2.2 1,938,411 + BT1574 0.34 +0.5 1,938,421 + BT1574 0.34 -2.1 1,938,454 + BT1574 0.37 -0.5 1,938,455 - BT1574 0.37 -0.9 1,938,461 - BT1574 0.37 -0.9 1,938,478 + BT1574 0.38 -1.2 1,938,495 + BT1574 0.40 -0.5 1,938,593 - BT1574 0.46 -0.5 1,938,613 + BT1574 0.48 -0.1 1,938,694 + BT1574 0.53 +1.1 1,938,717 - BT1574 0.55 -0.6 1,938,815 - BT1574 0.62 -0.9 1,938,817 - BT1574 0.62 -1.2 1,938,840 + BT1574 0.64 -0.5 1,938,879 + BT1574 0.66 -1.9 1,938,879 + BT1574 0.66 -2.1 1,938,883 + BT1574 0.67 -2.8 1,938,956 - BT1574 0.72 -0.9 1,939,029 - BT1574 0.77 -0.9 1,939,037 + BT1574 0.77 -3.4 1,939,103 - BT1574 0.82 -1.5 1,939,167 - BT1574 0.86 -1.9 1,939,211 - BT1574 0.90 -1.6 1,939,214 - BT1574 0.90 -0.9 1,939,286 - -2.2 1,939,395 + -1.2 1,939,419 + -0.5 1,939,419 + +0.6 1,939,433 + -0.5 1,939,566 + -3.1 1,939,664 - -0.9 1,939,815 + BT1575 0.24 -2.7 1,939,816 - BT1575 0.25 -0.9 1,939,900 - BT1575 0.33 -1.4 1,939,900 - BT1575 0.33 -2.2 1,940,357 - BT1575 0.79 -0.5
Or see this region's nucleotide sequence