Experiment: BHIS with Lithocholic 0.4 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0809 and BT0810 are separated by 4 nucleotides BT0810 and BT0811 overlap by 50 nucleotides BT0811 and BT0812 are separated by 191 nucleotides BT0812 and BT0813 are separated by 12 nucleotides
BT0809: BT0809 - hypothetical protein (NCBI ptt file), at 1,001,132 to 1,002,214
BT0809
BT0810: BT0810 - hypothetical protein (NCBI ptt file), at 1,002,219 to 1,002,596
BT0810
BT0811: BT0811 - putative tRNA/rRNA methyltransferase (NCBI ptt file), at 1,002,547 to 1,003,305
BT0811
BT0812: BT0812 - putative transcriptional regulator (NCBI ptt file), at 1,003,497 to 1,003,859
BT0812
BT0813: BT0813 - TonB (NCBI ptt file), at 1,003,872 to 1,005,203
BT0813
Position (kb)
1002
1003
1004 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1001.976 kb on + strand, within BT0809 at 1002.117 kb on + strand at 1002.240 kb on + strand at 1002.514 kb on + strand, within BT0810 at 1002.586 kb on + strand at 1002.587 kb on - strand at 1002.604 kb on + strand at 1002.605 kb on - strand at 1002.641 kb on + strand, within BT0811 at 1002.642 kb on - strand, within BT0811 at 1002.653 kb on + strand, within BT0811 at 1002.749 kb on + strand, within BT0811 at 1002.827 kb on + strand, within BT0811 at 1002.868 kb on + strand, within BT0811 at 1002.868 kb on + strand, within BT0811 at 1002.869 kb on - strand, within BT0811 at 1002.884 kb on + strand, within BT0811 at 1002.886 kb on + strand, within BT0811 at 1002.887 kb on - strand, within BT0811 at 1002.892 kb on + strand, within BT0811 at 1002.912 kb on + strand, within BT0811 at 1003.025 kb on - strand, within BT0811 at 1003.093 kb on + strand, within BT0811 at 1003.235 kb on - strand at 1003.235 kb on - strand at 1003.281 kb on - strand at 1003.301 kb on - strand at 1003.407 kb on + strand at 1003.836 kb on - strand at 1003.858 kb on - strand at 1003.868 kb on - strand at 1003.903 kb on + strand at 1003.937 kb on + strand at 1004.088 kb on - strand, within BT0813 at 1004.183 kb on + strand, within BT0813
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Lithocholic 0.4 mg/ml remove 1,001,976 + BT0809 0.78 -0.4 1,002,117 + -1.0 1,002,240 + -1.6 1,002,514 + BT0810 0.78 -1.2 1,002,586 + -0.4 1,002,587 - -1.8 1,002,604 + -1.4 1,002,605 - +0.8 1,002,641 + BT0811 0.12 -1.2 1,002,642 - BT0811 0.13 -2.8 1,002,653 + BT0811 0.14 +0.6 1,002,749 + BT0811 0.27 +0.2 1,002,827 + BT0811 0.37 -1.4 1,002,868 + BT0811 0.42 -1.8 1,002,868 + BT0811 0.42 -0.6 1,002,869 - BT0811 0.42 -2.3 1,002,884 + BT0811 0.44 -1.6 1,002,886 + BT0811 0.45 -2.0 1,002,887 - BT0811 0.45 -2.3 1,002,892 + BT0811 0.45 +0.2 1,002,912 + BT0811 0.48 -1.8 1,003,025 - BT0811 0.63 -1.6 1,003,093 + BT0811 0.72 +0.2 1,003,235 - -0.7 1,003,235 - -0.4 1,003,281 - -0.2 1,003,301 - +0.8 1,003,407 + +1.2 1,003,836 - -2.0 1,003,858 - -4.1 1,003,868 - +0.2 1,003,903 + -0.8 1,003,937 + +0.2 1,004,088 - BT0813 0.16 +1.2 1,004,183 + BT0813 0.23 -2.7
Or see this region's nucleotide sequence